Results for “engineer”

Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.

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Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

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Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.

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Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.

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Build a differential-ready consensus peakset from per-replicate ATAC-seq peaks using iterative overlap removal, fixed-width re-centering, and majority-rule overlap. Use when generating a stable peak coordinate system for downstream differential accessibility, ML feature engineering, cross-sample comparison, or fixed-width peak counts; covers Corces 2018 iterative overlap (501 bp), DiffBind summit re-centering, and ENCODE consistency rules.

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Generates Spring Boot 3.x configurations, creates REST controllers, implements Spring Security 6 authentication flows, sets up Spring Data JPA repositories, and configures reactive WebFlux endpoints. Use when building Spring Boot 3.x applications, microservices, or reactive Java applications; invoke for Spring Data JPA, Spring Security 6, WebFlux, Spring Cloud integration, Java REST API design, or Microservices Java architecture.

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You are a senior backend engineer operating production-grade services under strict architectural and reliability constraints. Use when routes, controllers, services, repositories, express middleware, or prisma database access.

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This skill enforces Sentry's blog writing standards across every post — whether you're helping an engineer write their first blog post or a marketer draft a product announcement.

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Senior embedded software engineer specializing in firmware and driver development for ARM Cortex-M microcontrollers (Teensy, STM32, nRF52, SAMD).

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Core UI/UX engineering skill for building highly interactive, spatial, weightless, and glassmorphism-based web interfaces using GSAP and 3D CSS.

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Systematic improvement of existing agents through performance analysis, prompt engineering, and continuous iteration.

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Build production-ready LLM applications, advanced RAG systems, and intelligent agents. Implements vector search, multimodal AI, agent orchestration, and enterprise AI integrations.

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Forces the laziest solution that actually works, simplest, shortest, most minimal. Channels a senior dev who has been paged at 3am for a factory class that wrapped one function: question whether the task needs to exist at all, reach for the standard library before custom code, native platform features before dependencies, one line before fifty. Supports intensity levels: lite, full, ultra. The default is full. Use on ANY coding task: writing, adding, refactoring, fixing, reviewing, or designing code, and choosing libraries or dependencies. Also use whenever the user says "ponytail", "be lazy", "lazy mode", "simplest solution", "minimal solution", "yagni", "do less", or "shortest path", or complains about over-engineering, bloat, boilerplate, or unnecessary dependencies. Do NOT use for non-coding requests such as general knowledge, prose, translation, summaries, recipes.

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CI/CD e automação — GitHub Actions, Terraform, OpenTofu, Ansible, n8n. Use quando o usuário disser "pipeline", "CI/CD", "GitHub Actions", "workflow", "terraform", "ansible", "n8n", "automatizar", "deploy automático", "IaC".

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Confiabilidade da plataforma — SLO, error budget, capacidade, resiliência, ponto único de falha e revisão de prontidão. Use quando o usuário disser "SLA", "SLO", "disponibilidade", "resiliência", "aguenta?", "single point of failure", "production readiness", "quanto de folga temos".

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Proxmox VE e PBS — cluster, quorum, Ceph, LXC, VM, HA, replicação, storage e backup. Use quando o usuário disser "proxmox", "PVE", "ceph", "LXC", "container LXC", "VM não sobe", "cluster sem quorum", "PBS", "backup da VM". Sem MCP nem CLI padrão instalada — opera via SSH no nó ou API REST.

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Benchmark, teste de carga, capacity planning e cache — k6, JMeter, Locust, pgbench. Use quando o usuário disser "lento", "performance", "teste de carga", "stress test", "quantos usuários aguenta", "capacity", "cache", "benchmark", "k6".

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React and Next.js performance optimization guidelines from Vercel Engineering. This skill should be used when writing, reviewing, or refactoring React/Next.js code to ensure optimal performance patterns. Triggers on tasks involving React components, Next.js pages, data fetching, bundle optimization, or performance improvements.

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When the user wants to plan, evaluate, or build a free tool for marketing purposes — lead generation, SEO value, or brand awareness. Also use when the user mentions "engineering as marketing," "free tool," "marketing tool," "calculator," "generator," "interactive tool," "lead gen tool," "build a tool for leads," "free resource," "ROI calculator," "grader tool," "audit tool," "should I build a free tool," or "tools for lead gen." Use this whenever someone wants to build something useful and give it away to attract leads or earn links. For downloadable content lead magnets (ebooks, checklists, templates), see lead-magnets.

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React and Next.js performance optimization guidelines from Vercel Engineering. This skill should be used when writing, reviewing, or refactoring React/Next.js code to ensure optimal performance patterns. Triggers on tasks involving React components, Next.js pages, data fetching, bundle optimization, or performance improvements.

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Route code cleanup, maintainability, React diagnostics, and over-engineering review to focused review modes. Use when auditing code quality or asking for a particular review lens.

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Sets up an `## Agent skills` block in AGENTS.md/CLAUDE.md and `docs/agents/` so the engineering skills know this repo's issue tracker (GitHub or local markdown), triage label vocabulary, and domain doc layout. Run before first use of `to-issues`, `to-prd`, `triage`, `diagnose`, `tdd`, `improve-codebase-architecture`, or `zoom-out` — or if those skills appear to be missing context about the issue tracker, triage labels, or domain docs.

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Forces the laziest solution that actually works, simplest, shortest, most minimal. Channels a senior dev who has seen everything: question whether the task needs to exist at all (YAGNI), reach for the standard library before custom code, native platform features before dependencies, one line before fifty. Supports intensity levels: lite, full (default), ultra. Use on ANY coding task: writing, adding, refactoring, fixing, reviewing, or designing code, and choosing libraries or dependencies. Also use whenever the user says "ponytail", "be lazy", "lazy mode", "simplest solution", "minimal solution", "yagni", "do less", or "shortest path", or complains about over-engineering, bloat, boilerplate, or unnecessary dependencies. Do NOT use for non-coding requests (general knowledge, prose, translation, summaries, recipes).

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React and Next.js performance optimization guidelines from Vercel Engineering. This skill should be used when writing, reviewing, or refactoring React/Next.js code to ensure optimal performance patterns. Triggers on tasks involving React components, Next.js pages, data fetching, bundle optimization, or performance improvements.

The English summary is being prepared. Showing the original description.