Results for “cfd”

Apply Rob Fitzpatrick's Mom Test discipline to customer interviews during PRD v1.0 Market Adoption. Triggers on requests to interview customers, run discovery calls, validate ideas with users, or when user asks "how do I interview customers?", "Mom Test", "Rob Fitzpatrick", "customer interview", "user research without lies", "talking to humans", "discovery conversation". Outputs CFD-* discovery entries with Mom Test-calibrated confidence.

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Systematically harvest short testimonials from customers via NPS, milestones, post-purchase, and email during PRD v1.0 Market Adoption. Triggers on requests to collect testimonials, set up social proof harvest, gather customer quotes, or when user asks "how do we get testimonials?", "social proof harvest", "NPS responses", "testimonial wall", "customer quotes", "post-purchase survey", "milestone follow-up". Outputs CFD-TST-* testimonial entries and GTM-TST-* placement assets.

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Generates simulation inputs across fidelity levels — OpenFOAM CFD cases, ngspice netlists, interactive React artifacts, and FreeCAD FEM setups — from verified engineering calculations.

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Design and analyze engineering experiments or simulations using baseline cases, hypothesis-driven DOE, traceable data processing, defensible plots, uncertainty, and validation. Use for CFD, laboratory data, ML workflows, parameter studies, or research code.

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Apply source-aware mechanical-engineering judgment to research, analysis, coding, writing, teaching, research identity, and release work. Use for thermal-fluid systems, heat transfer, fluid mechanics, thermodynamics, HVAC, energy systems, turbomachinery, piping, multiphase flow, experiments, correlations, CFD, reduced-order models, AI/ML, uncertainty, engineering datasets, literature reviews, citations, manuscripts, reviewer revisions, Overleaf packages, figures, proposals, research software, reproducibility, public releases, engineering teaching materials, or a research-project logo and visual identity.

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Analyze thermal-fluid engineering systems, experiments, CFD, and correlations with explicit assumptions, validity limits, uncertainty, and mechanism-based interpretation. Use for heat transfer, fluid mechanics, phase change, HVAC, energy systems, turbomachinery, piping, or thermal management.

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Analyze carbon accounting and emissions tracking software for Scope 1/2/3 calculation accuracy, GHG Protocol compliance, offset verification, supply chain emissions, reporting standards (CDP, TCFD, GRI, SASB, SEC), reduction target tracking, and audit trail integrity..

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Detects somatic mutations in circulating tumor DNA, treating low-VAF detection as a signal-versus-noise problem set by error suppression and molecules sampled, not by the choice of caller. Distinguishes de novo CALLING (scanning a panel for unknown variants, bounded by per-locus error and multiple testing) from tumor-informed DETECTION (tracking a pre-specified variant set, where panel integration reaches single-ppm). Covers VarDict and Mutect2 for de novo calling, UMI-aware callers, and a pysam-based known-variant VAF tracker, with matched-WBC subtraction as the mandatory defense against clonal hematopoiesis (the dominant false positive). Use when calling or tracking tumor mutations from plasma cfDNA, setting a VAF threshold, or deciding whether a low-VAF call is tumor versus CHIP.

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Designs pooled sgRNA libraries for CRISPR knockout, interference (CRISPRi), activation (CRISPRa), Cas12a multiplex, base-editor, and prime-editor screens. Covers on-target scoring (Rule Set 2, Azimuth, DeepSpCas9, CRISPRon), off-target scoring (CFD, MIT), TSS-relative positioning for CRISPRi/a (Horlbeck, Dolcetto, Calabrese), PAM-variant chemistries, control-guide composition, oligo cloning architecture, and library QC. Use when choosing a genome-wide library (GeCKOv2 vs Avana vs Brunello vs TKOv3 vs Inzolia), designing a focused or paralog-focused custom library, picking CRISPRi vs CRISPRa TSS windows, deciding control-guide proportions, or diagnosing library skew and dropout in a freshly cloned pool.

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Knowledge base from the GAO Agile Assessment Guide (GAO-24-105506, 2023). Use for assessing and managing Agile software development in federal programs: the Agile Manifesto and framework family (Scrum, Kanban, XP, SAFe, DA, DSDM, Lean, DevOps); the three adoption perspectives (team dynamics, program operations, organization environment); requirements decomposition (epics, features, user stories, INVEST, MoSCoW, definition of done); Agile-aware federal contracting (FAR, modular contracting, SOO/PWS/SOW, product owner vs. COR); program monitoring and control (WBS, cost, schedule, EVM at the feature level); Agile metrics (velocity, lead/cycle time, CFD, burn charts); and the auditor's key-question bank. Oversight- and acquisition-oriented: it tells you how to *assess and govern* Agile programs, not how to code. Thin on hands-on engineering practice, deep cost/schedule checklists (see gao-cost, gao-schedule), and any non-US-federal context.

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Computational fluid dynamics — Navier-Stokes solvers, lid-driven cavity, channel flow, vortex methods, turbulence statistics, drag/lift computation. Spectral and finite-difference methods for incompressible and compressible flows.

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Patterns for loading PDE simulation datasets (PDEBench, PhiFlow, JAX-CFD) from HDF5 files. Handles layout detection (single tensor vs separate variables), spatial/temporal downsampling, multi-variable systems, HuggingFace and DaRUS data sources, and efficient PyTorch DataLoader creation. Use when preparing PDE data for neural operator training.

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Cell-free DNA analysis pipeline from plasma sequencing to tumor monitoring. Preprocesses cfDNA reads, analyzes fragment patterns, estimates tumor fraction from sWGS, and optionally detects mutations from targeted panels. Use when analyzing liquid biopsy samples for cancer detection or monitoring.

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Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.

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Designs pooled sgRNA libraries for CRISPR knockout, interference (CRISPRi), activation (CRISPRa), Cas12a multiplex, base-editor, and prime-editor screens. Covers on-target scoring (Rule Set 2, Azimuth, DeepSpCas9, CRISPRon), off-target scoring (CFD, MIT), TSS-relative positioning for CRISPRi/a (Horlbeck, Dolcetto, Calabrese), PAM-variant chemistries, control-guide composition, oligo cloning architecture, and library QC. Use when choosing a genome-wide library (GeCKOv2 vs Avana vs Brunello vs TKOv3 vs Inzolia), designing a focused or paralog-focused custom library, picking CRISPRi vs CRISPRa TSS windows, deciding control-guide proportions, or diagnosing library skew and dropout in a freshly cloned pool.

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Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.

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Designs pooled sgRNA libraries for CRISPR knockout, interference (CRISPRi), activation (CRISPRa), Cas12a multiplex, base-editor, and prime-editor screens. Covers on-target scoring (Rule Set 2, Azimuth, DeepSpCas9, CRISPRon), off-target scoring (CFD, MIT), TSS-relative positioning for CRISPRi/a (Horlbeck, Dolcetto, Calabrese), PAM-variant chemistries, control-guide composition, oligo cloning architecture, and library QC. Use when choosing a genome-wide library (GeCKOv2 vs Avana vs Brunello vs TKOv3 vs Inzolia), designing a focused or paralog-focused custom library, picking CRISPRi vs CRISPRa TSS windows, deciding control-guide proportions, or diagnosing library skew and dropout in a freshly cloned pool.

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