Results for “replicate”

Publication-figure overlay for draw.io. Use instead of drawio whenever the diagram is for a paper, thesis, dissertation, journal, conference, IEEE/ACM submission, manuscript, camera-ready, Word/LaTeX figure, or other publication. Applies venue, figure-type, color, caption/legend, formula, and paper-readability gates for architecture, workflow, roadmap, network-topology, and replicated paper figures.

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Create, edit, replicate, import, and export draw.io diagrams with an offline YAML-first workflow: architecture, network topologies, flowcharts, UML/ER, org charts, Mermaid/CSV conversion, existing .drawio bundles, style presets, themes, and non-publication formula diagrams. For publication figures (paper, thesis, IEEE, camera-ready) use drawio-academic-skills instead.

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Convert image references into spatial hypotheses, camera constraints, construction routes, material evidence, and measurable Blender reconstruction gates. Use whenever a user asks to replicate, recreate, match, clone, or reconstruct a scene, environment, object, material, lighting setup, or shot from one or more images, especially when room connectivity, hidden space, perspective, visible-frame fidelity, material identity, aging, wetness, or photographic treatment matters.

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Extract the design of a screenshot, website URL or Figma file into a design.md with tokens, components, layout and brand rules, or copy one element (navbar, card, illustration) into an element.md with a rebuild or image-generation prompt. Use when the user wants to document, replicate, audit or reverse-engineer a visual design: its design system, palette, typography or style.

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Think and work like an expert Molecular Biologist. Use when a task calls for Molecular Biologist judgment. Reasons from central-dogma sequence flow, binding affinity (Kd/Km/kcat), gene regulation, and biological-versus-technical replicate structure through MIQE-compliant RT-qPCR, ddPCR, Western/flow/microscopy, CRISPR editing with rescue, and IWGAV antibody validation while treating off-target reagent effects, batch effects, mycoplasma and cell-line misidentification, and toxicity-driven artifacts as first-class failure modes.

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Think and work like an expert Crop Scientist. Use when a task calls for Crop Scientist judgment. Reasons from genotype-by-environment-by-management interaction, yield-component partitioning, and phenology-gated critical periods through MET stability analysis (AMMI, Finlay-Wilkinson, GGE biplots), mixed models (ASReml-R, lme4), N-response curves (quadratic-plateau, MRTN), and crop models (APSIM, DSSAT) while treating pseudo-replicated subsamples, single-site yield champions, uncorrected harvest moisture, and weather-during-anthesis confounding as first-class failure modes.

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Think and work like an expert Community Ecologist. Use when a task calls for Community Ecologist judgment. Reasons from Vellend's four processes and Chesson stabilizing/equalizing coexistence through PERMANOVA/betadisper, betapart turnover–nestedness, Gotelli SIM9/C-score null models, and vegan/entropart/picante pipelines while treating compositional closure, dispersion heterogeneity, and pseudoreplicated quadrats as first-class failure modes.

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Think and work like an expert Animal Nutritionist. Use when a task calls for Animal Nutritionist judgment. Reasons from NASEM/AAFCO requirements, SID amino acids and ideal protein, CNCPS MP/NE balance, and NANP feed libraries through NIR-wet chemistry validation, ileal vs fecal digestibility, pen-structured mixed models, and ARRIVE reporting while treating intake confounds, NIR calibration drift, acidosis/sorting, and pseudoreplicated pens as first-class failure modes.

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Compare evaluated runs in a retort experiment along factor dimensions. Surfaces effects of each factor, aggregates across replicates, and highlights cells that diverge qualitatively — complementing (not replacing) retort's ANOVA analysis.

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Production guidance for international BytePlus Seed Speech text-to-speech. Use for selecting TTS 1.0 versus 2.0, bidirectional or unidirectional streaming, current voices and languages, prompt/prosody controls, subtitle timing validation, billing and concurrency, authorized replicated voices, privacy, error handling, and output QA. Do not use for mainland-China Volcengine Doubao Speech endpoints.

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Select, integrate, and operate multi-model video-generation gateways — hosted inference aggregators such as fal.ai, Replicate, and WaveSpeed that expose many third-party video (and video-adjacent audio) models behind one account, one API surface, and one bill. Use when deciding whether to route video generation through a gateway versus a direct model-provider API; when comparing gateways on catalog, async/queue job design, webhooks, pricing, schema discovery, and version pinning; and when operating gateway workloads in production — spend controls, retries, cross-gateway failover, content- safety differences, data retention, and commercial-rights passthrough of the underlying model. Not for direct model-provider APIs, local/self-hosted inference, model training/fine-tuning, or image-only gateway use.

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Select, integrate, and operate multi-model image-generation gateways with model-specific schema discovery, version policy, asynchronous jobs, webhooks, spend approval, safe inputs and artifacts, data-governance review, and billing observability. Use for comparing or building against fal.ai, Replicate, or Together AI image APIs, including controlled failover; do not use for direct model-provider APIs, local inference, training, dedicated endpoint provisioning, video generation, or general image editing.

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Build a differential-ready consensus peakset from per-replicate ATAC-seq peaks using iterative overlap removal, fixed-width re-centering, and majority-rule overlap. Use when generating a stable peak coordinate system for downstream differential accessibility, ML feature engineering, cross-sample comparison, or fixed-width peak counts; covers Corces 2018 iterative overlap (501 bp), DiffBind summit re-centering, and ENCODE consistency rules.

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Identify differentially bound regions across CLIP-seq conditions (knockdown vs control, treatment vs vehicle, disease vs healthy) using DEWSeq (sliding-window DESeq2), Flipper (Skipper-downstream), ASpeak, edgeR, or limma-voom. Use when computing condition-level changes in RBP binding intensity, choosing peak-level vs window-level vs crosslink-level testing, designing replicate experiments, or distinguishing biological binding shifts from technical confounders.

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End-to-end pooled and single-cell CRISPR screen analysis from FASTQ to hit genes. Orchestrates library design QC, guide counting, six-stage screen QC (plasmid Gini, replicate Pearson, CEGv2 PR-AUC, copy-number artifact), method-appropriate hit calling across MAGeCK RRA/MLE, BAGEL2, drugZ, JACKS, and Chronos, cancer-cell-line copy-number correction (CRISPRcleanR / Chronos), batch correction for multi-batch screens, and the specialized branches for combinatorial paralog screens, single-cell Perturb-seq, base-editor variant-function screens, prime-editor screens, and in vivo bottleneck-aware screens. Use when analyzing any pooled CRISPR screen end-to-end, choosing the correct hit-calling method by experimental design, integrating copy-number correction into the pipeline, or branching the workflow for single-cell, combinatorial, base-editor, prime-editor, or in vivo variants.

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Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.

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ATAC-seq library quality control -- TSS enrichment, FRiP, fragment-size periodicity, library complexity (NRF/PBC1/PBC2), mitochondrial fraction, and ENCODE 4 thresholds. Use when assessing whether an ATAC-seq library passes ENCODE acceptance criteria, diagnosing transposition artefacts, comparing Omni-ATAC vs standard prep quality, or selecting which replicates to drop before peak calling.

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Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.

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Generates paste-ready Power Apps Canvas App YAML. Invoke when the user wants to replicate a UI mockup, improve an existing Canvas app screen, or build a new screen from a text description. Also invoke when the user asks to "improve", "redesign", or "generate YAML" for a Canvas app screen.

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Use when the user asks for Superloopy clone or asks to clone, rebuild, reverse-engineer, replicate, or copy a website or page into a Superloopy-governed implementation. Triggers on "loopy clone", target URLs plus requests such as "clone this site", "rebuild this page", "make a copy of this website", "pixel-perfect clone", or "AI website clone". Requires browser automation and records component specs, assets, implementation, build output, and visual QA as Superloopy evidence.

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爆款拆解复刻：把抖音/TikTok 爆款带货视频（链接、转写或口述结构）拆成 8 维结构与 5-beat 节拍，再映射到你的商品，输出拆解表 + 3 个复刻角度 + 可渲染 storyboard.json。Use when the user says 爆款, 拆解, 复刻, 对标, 仿拍, viral remake, breakdown, swipe file, copy structure, 竞品视频, remake a TikTok/Douyin product video, or wants to replicate a viral ecommerce short's structure with their own product.

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Distributed consensus algorithms and logical time for cloud and multi-node systems. Covers Lamport clocks, vector clocks, FLP impossibility, Paxos (basic, multi, fast), Raft, Viewstamped Replication, Byzantine fault tolerance basics, quorum reads/writes (N/R/W), leader election, and TLA+ specification style. Use when designing replicated state machines, picking a consensus protocol, reasoning about split-brain and quorum loss, or writing formal specs for distributed coordination.

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Ultimate Design System Intelligence. Manage, extract, replicate, and improve UI design systems. Extracts design tokens visually from existing sites (Harvester v5), manages multi-page tokens, and utilizes MCP servers like Google Stitch and Pencil.dev to generate complete, consistent design systems (Shadcn UI, Halo, Lunaris, Nitro). Use when the user asks to "extract design from a URL", "create a Shadcn design system", "analyze design tokens", "build a design system for", or "copy the UI style of".

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视频逆向复刻 — 分析参考视频(FFmpeg帧提取+Vision LLM) + 生成复刻Seedance 2.0 Prompt + 4种素材替换模式。支持单个和批量。Use when: '复刻这个视频', '分析爆款视频', 'replicate this video', '视频逆向', '反编译视频', '批量分析视频'. Do NOT use for creating from scratch — use fashion-video-creator instead.

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