Results for “replicate”

Reverse-engineer raw materials (Sparse idea, Dense idea, experimental log) from an existing AI research paper to build a benchmark case for evaluating paper-writing pipelines. Replicates the PaperWritingBench dataset construction procedure from arXiv:2604.05018 §3 / App. C. TRIGGER when the user asks to "build a benchmark case from this paper", "reverse-engineer raw materials", or "evaluate my pipeline against PaperWritingBench".

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把一个目标/主题/文章/截图/数据转成有观点、有节奏、有视觉约束的单文件 HTML 演示（网页 PPT、科技战报、产品拆解、行业观察、路演/发布会 deck、社媒可传播长图）。**7 套各锚定公认设计体系的视觉人格**——技术崇高派 Technical Sublime（工程冷光·技术战报/算力）/ 构成主义 Constructivism（宣言/号召）/ 信息·数据设计（Tufte·数据/图表）/ 判断震慑极简 Luxury（高端品牌/判断）/ De Stijl 新造型（标准/秩序）/ 编辑主义 Editorial（衬线·深度观察）/ 演示禅 Zen（暗墨·思想/品牌 keynote）——用户可指定，否则按内容自动判选并透明告知。token + validator 机检（P0/P1/P2）+ 32 维审计**机器强制设计一致性**；反伪造（不造数据/logo/截图/来源·示意标注）；字体本地子集；图按角色路由（默认 CSS/SVG·要图先探测生图环境）。Generates single-file HTML decks with 7 design-system-anchored visual personas, a semantic layout grammar, locked design tokens, evidence-grading, machine validation (P0/P1/P2 + 32-dimension audit), an anti-fabrication discipline, and image-generation environment detection. Use when the user wants a presentation / deck / slides / 网页 PPT / 战报 / 产品拆解 / 行业观察 / 路演 / 发布会 / 社媒长图, or asks to turn an article / screenshot / dataset into slides. Do NOT use for large data-table training material, strict legal/medical/financial compliance documents, multi-author collaborative PPT editing, plain-text summaries, requests to replicate a brand's official keynote visuals, or any request to fabricate realistic-looking data / fake logos / fake screenshots / fake sources.

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"Apply the Resource-Based View (Barney, 1991) and VRIO framework to evaluate whether a firm's resources and capabilities confer sustained competitive advantage. Use this skill when the user needs to assess internal resources for strategic value, determine if a competitive edge is sustainable, audit resource portfolios for VRIO criteria, or when they ask 'what makes our advantage sustainable', 'which resources matter most', or 'can competitors replicate this'.".

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Before designing, training, or auditing ANY model that replicates human-annotated labels, audit the annotation protocol's INPUT — the exact document/evidence the human labelers consulted — and give the model that same input. Use when: (1) designing a classifier/LLM extractor whose target is a hand-coded label set, (2) a label-replication model shows low recall concentrated in a label subset and the diagnosis on offer is "the label's information is not in the features", (3) reviewers propose construct splits (e.g. "designation vs record-evident"), adjudication sittings, or per-domain stop rules to explain residual disagreement with gold, (4) validating an extraction pipeline against labels transcribed from a source document. Symptom of the underlying failure: elaborate theory accumulates to explain why gold is "partially unpredictable" when the model was simply never shown the document the annotators read.

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Generates networking boilerplate adapted to the project's chosen networking package (NGO, Mirror, Photon). Use when setting up multiplayer systems, syncing state, writing RPCs, building lobbies, or designing an authority matrix. Triggers on requests like "add multiplayer", "sync player health", "create an RPC", "set up lobbies", "make this networked", "add co-op", "replicate this across clients", "spawn networked objects", "handle player disconnect", "create a matchmaking system", or any task involving network state synchronization, remote procedure calls, or client-server architecture. Always reads ProjectConfig.yaml -> networking before generating code. If networking is "none", do NOT generate networking code.

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Access ENCODE uniform analysis pipelines, generate user-specific Nextflow/WDL pipelines, manage compute resources, and integrate with cloud platforms. Use when the user wants to understand ENCODE pipelines, run pipelines on their own data, generate custom Nextflow workflows from ENCODE pipeline code, check compute requirements (CPU/GPU/memory), run pipelines in background, or integrate with Google Cloud, AWS, or other cloud platforms. Also use when the user asks about ENCODE pipeline outputs, processing standards, software versions, or wants to replicate ENCODE processing. Covers local execution, HPC, and cloud deployment with resource-aware scheduling. Use this skill for ANY pipeline execution, workflow generation, or compute resource management task involving ENCODE data.

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Pixel-faithful clone of any web UI into the user's existing stack, using whatever sources are available — a screenshot alone, a live URL, raw HTML/CSS, or any combination. Use this skill whenever the user wants to recreate, match, replicate, or "clone" a design from a screenshot, image, URL, Figma export, or HTML dump. Trigger on phrases like "clone this", "match this design", "build this from screenshot", "recreate this page", "make it look like this", "rebuild this UI", "copy this layout", or any time the user provides a visual reference and asks for a faithful implementation. Do not undertrigger — even if the user just drops a screenshot without explicit phrasing, this skill applies.

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Structures biological experiments so inference is valid by construction, covering Fisher's principles (randomization, replication, local control), the experimental-vs-observational unit distinction and pseudoreplication (Hurlbert 1984; Lazic 2018), randomization mechanics (complete, restricted, stratified, rerandomization, run-order), blocking layouts (randomized complete block, Latin square, incomplete block), factorial designs and interactions, and the split-plot/nested error strata hidden inside multi-batch genomics. Use when deciding the experimental unit and what counts as a replicate, planning randomization and run order, choosing a blocked/factorial/split-plot/nested layout, avoiding pseudoreplication in cell-culture or animal studies, or specifying the random-effects structure of the analysis model. For assigning samples to sequencing batches/lanes/plates and batch-effect correction see experimental-design/batch-design; for regulated clinical-trial randomization see clinical-biostatistics.

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Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.

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Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.

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Identify differentially bound regions across CLIP-seq conditions (knockdown vs control, treatment vs vehicle, disease vs healthy) using DEWSeq (sliding-window DESeq2), Flipper (Skipper-downstream), ASpeak, edgeR, or limma-voom. Use when computing condition-level changes in RBP binding intensity, choosing peak-level vs window-level vs crosslink-level testing, designing replicate experiments, or distinguishing biological binding shifts from technical confounders.

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Calls ChIP-seq peaks with MACS3, MACS2, HOMER, or SPP across narrow (TF) and broad (histone) modes. Handles input control matching, fragment-size modeling vs --nomodel, effective genome size, ENCODE-style IDR vs naive overlap, hyper-ChIPable artifacts, and aligner-specific shifts. Use when calling peaks from ChIP-seq alignments, choosing between narrow vs broad mode for a histone mark, deciding model vs nomodel for low-depth data, applying ENCODE pseudoreplicate IDR, or reconciling MACS vs HOMER vs SPP results.

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Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.

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Build a differential-ready consensus peakset from per-replicate ATAC-seq peaks using iterative overlap removal, fixed-width re-centering, and majority-rule overlap. Use when generating a stable peak coordinate system for downstream differential accessibility, ML feature engineering, cross-sample comparison, or fixed-width peak counts; covers Corces 2018 iterative overlap (501 bp), DiffBind summit re-centering, and ENCODE consistency rules.

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ATAC-seq library quality control -- TSS enrichment, FRiP, fragment-size periodicity, library complexity (NRF/PBC1/PBC2), mitochondrial fraction, and ENCODE 4 thresholds. Use when assessing whether an ATAC-seq library passes ENCODE acceptance criteria, diagnosing transposition artefacts, comparing Omni-ATAC vs standard prep quality, or selecting which replicates to drop before peak calling.

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Treats a ctDNA assay as a molecule-counting experiment at the Poisson edge and builds its analytical-validation case the measurement-science way. Covers the genome-equivalent currency (~330 haploid copies/ng), the lambda = input_GE x VAF sampling ceiling (lambda>=3 for ~95% detection), the error-suppression ladder (raw NGS ~1e-3 -> single-strand UMI ~1e-4/1e-5 -> duplex <1e-7), the CLSI EP17 LoB/LoD/LoD95/LoQ framework, the per-locus-vs-panel-integrated LoD distinction that lets bespoke MRD reach ppm, contrived/SEQC2 reference standards, and honest LoD reporting conditioned on input mass + consensus depth + replicate detection rate. Use when stating or trusting a sensitivity claim, designing a dilution-series validation, deciding how many genome equivalents are needed at a target VAF, choosing a single-locus vs panel-integrated LoD, or auditing a "detects 0.1% VAF" claim.

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Copies or adapts features from external repos/apps into this project. Triggers on 'copy this from', 'replicate'. NOT for packing this project (mk:pack); NOT for URL fetches (mk:web-to-markdown).

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Implement replicated appearance data, GenericVisualizer mappings, sprite layers, overlays, and client visual systems.

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AI image generation with OpenAI GPT Image 2, Azure OpenAI, Google, OpenRouter, DashScope, Z.AI GLM-Image, MiniMax, Jimeng, Seedream, Replicate and Agnes APIs. Supports text-to-image, reference images, aspect ratios, and batch generation from saved prompt files. Sequential by default; use batch parallel generation when the user already has multiple prompts or wants stable multi-image throughput. Use when user asks to generate, create, or draw images.

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Perform DCSync attacks to replicate Active Directory credentials and establish domain persistence by extracting KRBTGT, Domain Admin, and service account hashes for Golden Ticket creation.

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Operating Rojo — the filesystem-to-Roblox-Studio sync tool for professional Roblox development in VS Code / Claude Code instead of the Studio editor. Use this skill whenever Rojo is involved: `rojo serve`/`rojo build`, writing or debugging `default.project.json`, rokit/rokit.toml and tool versions (Rojo, Lune, Wally), nested vs. flat path mapping (ReplicatedStorage.Project.shared), connect/port/sync problems, or when a Roblox project skeleton needs to be created. Also trigger on "rojo connect not working", "scripts end up in the wrong place in Studio", "how do I map src/ to Studio", "port 34872 in use", "ModuleScript vs Script in Rojo".

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Create and process videos using AI generation (Runway, Pika, Replicate, Sora, Veo) and FFmpeg processing. MCP bridge provides frame extraction (extract_video_frames); the Design Stack media worker handles full FFmpeg processing (resize, trim, compress, GIF, format conversion). Covers social-media-optimized video output. Use when generating AI videos, extracting frames/thumbnails, transcoding videos, creating GIFs from clips, or preparing video for social platforms.

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Perform DCSync attacks to replicate Active Directory credentials and establish domain persistence by extracting KRBTGT, Domain Admin, and service account hashes for Golden Ticket creation.

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Copy a feature flag from one PostHog project to one or more target projects in the same organization. Use when the user wants to duplicate a flag, promote a flag from staging to production, sync flags across projects, or replicate a flag configuration in a different workspace. Covers cohort remapping, scheduled-change handling, encrypted payloads, and the safe defaults (disabled in target, no scheduled changes).

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