Results for “annotation”

Gets actor and level 3D bounding boxes and projects them into a camera image as 2D boxes — the dataset-annotation workflow. Lists box geometry, draws 3D boxes in the world via debug, or captures one camera frame and writes an annotated PNG plus a JSON of 2D boxes for matching actors. Use when the user asks to "get bounding boxes", "draw boxes around the cars", "project boxes onto the camera", or "generate a detection dataset / annotations".

The English summary is being prepared. Showing the original description.

Parses, queries, converts, and extracts from GTF and GFF3 gene-model annotation files - walking the gene/transcript/exon/CDS hierarchy with gffutils (queryable SQLite DB), converting formats and extracting transcript/CDS/protein FASTA with gffread, slurping to dataframes with gtfparse/pyranges, and sanitizing malformed files with AGAT. Covers the 1-based-inclusive vs 0-based BED coordinate conversion (start-1 only), deriving implicit features (introns/UTRs/TSS), phase-not-frame, the stop-codon-in-or-out-of-CDS convention, and the chr1-vs-1 seqid and gene-ID-version mismatches that silently produce all-zero count matrices and dropped joins. Use when extracting features or sequences from an annotation, converting GTF<->GFF3 or GTF->BED, traversing the gene tree, or diagnosing a coordinate/provenance mismatch upstream of counting or DE.

The English summary is being prepared. Showing the original description.

Predicts protein-coding gene structures (exons, introns, UTRs) in eukaryotic genomes with BRAKER3 (RNA-seq + protein evidence), BRAKER1/BRAKER2, GALBA (protein-only), Funannotate (fungi), GeMoMa (homology projection), or Helixer/Tiberius (deep-learning ab initio). Covers the evidence-first tool decision, mandatory soft-masking, the training-set-quality-dominates principle, OrthoDB clade-partition selection, the one-isoform-per-locus and missing-UTR traps, merge/split errors, and reference bias against orphan genes. Use when annotating a newly assembled eukaryotic genome, choosing a gene-prediction pipeline based on available evidence, or diagnosing a poor annotation.

The English summary is being prepared. Showing the original description.

Discovers, classifies, and masks repetitive elements and transposable elements with RepeatModeler2 (de novo family library), RepeatMasker (masking against a library), EDTA (plant/structural TEs), or EarlGrey (auto-curating wrapper), and quantifies TE expression from RNA-seq with TEtranscripts/SQuIRE. Covers de-novo-library-as-curation-project, soft-vs-hard masking, the domesticated-gene over-masking massacre, Dfam-vs-RepBase, TE classification (Class I/II, family-vs-copy), Kimura repeat landscapes, LAI, and the RNA-seq multimapping problem. Use when masking repeats before gene prediction, building a TE library for a non-model genome, or analyzing transposable-element content or expression.

The English summary is being prepared. Showing the original description.

Model gene-family birth-death dynamics across a species tree using CAFE5 (Mendes et al 2020 Bioinformatics 36:5516 gamma-distributed rate categories), CAFE5-error (annotation-error-aware), Count (Csurös 2010 ancestral state reconstruction), BadiRate (Librado 2012 likelihood + parsimony), DupliPHY-Family, and ALE/AleRax (for per-family DTL; see [[gene-tree-species-tree-reconciliation]]). Test lineage-specific gene-family expansions and contractions, distinguish biological dynamics from annotation artifacts, account for assembly fragmentation, identify functional enrichment in expanded / contracted families. Use when correlating gene-family changes with phenotype evolution, ranking lineages by adaptive gene-family-rate shifts, post-WGD dosage-balance analysis, or building Birth-death models from OrthoFinder presence/absence matrices.

The English summary is being prepared. Showing the original description.

Annotate copy number variant segments with overlapping genes, dosage-sensitivity scores, cancer driver databases, population frequencies, and clinical-variant content. Covers bedtools/pybedtools interval intersection, AnnotSV comprehensive annotation and ranking, ClinGen haploinsufficiency/triplosensitivity scoring, gnomAD-SV/DGV frequency filtering, COSMIC Cancer Gene Census, and ClinVar overlap. Use when interpreting which genes a CNV affects, distinguishing the driver gene of a focal event from passengers, filtering against population CNVs, separating whole-gene from partial-gene overlap, or preparing CNVs for clinical classification.

The English summary is being prepared. Showing the original description.

Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

The English summary is being prepared. Showing the original description.

Discovers, classifies, and masks repetitive elements and transposable elements with RepeatModeler2 (de novo family library), RepeatMasker (masking against a library), EDTA (plant/structural TEs), or EarlGrey (auto-curating wrapper), and quantifies TE expression from RNA-seq with TEtranscripts/SQuIRE. Covers de-novo-library-as-curation-project, soft-vs-hard masking, the domesticated-gene over-masking massacre, Dfam-vs-RepBase, TE classification (Class I/II, family-vs-copy), Kimura repeat landscapes, LAI, and the RNA-seq multimapping problem. Use when masking repeats before gene prediction, building a TE library for a non-model genome, or analyzing transposable-element content or expression.

The English summary is being prepared. Showing the original description.

Parses, queries, converts, and extracts from GTF and GFF3 gene-model annotation files - walking the gene/transcript/exon/CDS hierarchy with gffutils (queryable SQLite DB), converting formats and extracting transcript/CDS/protein FASTA with gffread, slurping to dataframes with gtfparse/pyranges, and sanitizing malformed files with AGAT. Covers the 1-based-inclusive vs 0-based BED coordinate conversion (start-1 only), deriving implicit features (introns/UTRs/TSS), phase-not-frame, the stop-codon-in-or-out-of-CDS convention, and the chr1-vs-1 seqid and gene-ID-version mismatches that silently produce all-zero count matrices and dropped joins. Use when extracting features or sequences from an annotation, converting GTF<->GFF3 or GTF->BED, traversing the gene tree, or diagnosing a coordinate/provenance mismatch upstream of counting or DE.

The English summary is being prepared. Showing the original description.

Predicts protein-coding gene structures (exons, introns, UTRs) in eukaryotic genomes with BRAKER3 (RNA-seq + protein evidence), BRAKER1/BRAKER2, GALBA (protein-only), Funannotate (fungi), GeMoMa (homology projection), or Helixer/Tiberius (deep-learning ab initio). Covers the evidence-first tool decision, mandatory soft-masking, the training-set-quality-dominates principle, OrthoDB clade-partition selection, the one-isoform-per-locus and missing-UTR traps, merge/split errors, and reference bias against orphan genes. Use when annotating a newly assembled eukaryotic genome, choosing a gene-prediction pipeline based on available evidence, or diagnosing a poor annotation.

The English summary is being prepared. Showing the original description.

Annotate copy number variant segments with overlapping genes, dosage-sensitivity scores, cancer driver databases, population frequencies, and clinical-variant content. Covers bedtools/pybedtools interval intersection, AnnotSV comprehensive annotation and ranking, ClinGen haploinsufficiency/triplosensitivity scoring, gnomAD-SV/DGV frequency filtering, COSMIC Cancer Gene Census, and ClinVar overlap. Use when interpreting which genes a CNV affects, distinguishing the driver gene of a focal event from passengers, filtering against population CNVs, separating whole-gene from partial-gene overlap, or preparing CNVs for clinical classification.

The English summary is being prepared. Showing the original description.

Model gene-family birth-death dynamics across a species tree using CAFE5 (Mendes et al 2020 Bioinformatics 36:5516 gamma-distributed rate categories), CAFE5-error (annotation-error-aware), Count (Csurös 2010 ancestral state reconstruction), BadiRate (Librado 2012 likelihood + parsimony), DupliPHY-Family, and ALE/AleRax (for per-family DTL; see [[gene-tree-species-tree-reconciliation]]). Test lineage-specific gene-family expansions and contractions, distinguish biological dynamics from annotation artifacts, account for assembly fragmentation, identify functional enrichment in expanded / contracted families. Use when correlating gene-family changes with phenotype evolution, ranking lineages by adaptive gene-family-rate shifts, post-WGD dosage-balance analysis, or building Birth-death models from OrthoFinder presence/absence matrices.

The English summary is being prepared. Showing the original description.

Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

The English summary is being prepared. Showing the original description.

Use this skill when a driver needs to edit their own ELD record. Covers what edits are allowed, what edits trigger annotations, the unassigned-driving-time procedure, and when carriers must accept or reject driver edits.

The English summary is being prepared. Showing the original description.

Analyze development-set disagreements between human annotations and one Coval text LLM judge, then propose a focused prompt revision. Use coval-calibrate-metric for independent trust measurements.

The English summary is being prepared. Showing the original description.

Adds accessibility annotations (ARIA labels, roles, alt text) to make web content accessible. Use when user asks to "add accessibility", "make accessible", "add aria labels", "wcag compliance", or "screen reader support".

The English summary is being prepared. Showing the original description.

Complete guide for AutoFixture advanced customization techniques. Use when you need to customize AutoFixture builders or handle special type test data generation rules. Covers DataAnnotations automatic integration, ISpecimenBuilder implementation, priority management. Includes DateTime/numeric range builders, generic design, and fluent extension methods. Keywords: autofixture customization, autofixture customize, autofixture customization, specimen builder, ISpecimenBuilder, RandomDateTimeSequenceGenerator, NumericRangeBuilder, DataAnnotations autofixture, fixture.Customizations, Insert(0), custom builder, NoSpecimen, generic builder

The English summary is being prepared. Showing the original description.

[BETA] AEM Cloud Service expert skill — migrate javax.inject.@Inject fields in Sling Model (@Model) classes to injector-specific annotations (@ValueMapValue / @OSGiService / @SlingObject). Self-discoverable (scan for @Model classes with field-level @Inject); the replacement is chosen deterministically from each field's declared type. Use for "fix @Inject in HeroModel.java", "modernize my Sling Models", or when scanning an AEM project for @Inject misuse. Detailed decision table, companion-annotation handling, import management, and file-level skip policy are in recipe.md. This skill is in beta. Verify all outputs before applying them to production projects.

The English summary is being prepared. Showing the original description.

Design and review Java database code using MyBatis and MyBatis-Spring. Covers mapping query results to objects, safe SQL parameters, transactions, pagination, batch writes, and investigating slow or unnecessarily repeated queries.

• Designing mapper interfaces and XML mappings
• Reviewing dynamic SQL and parameter safety
• Checking transactions across multiple updates

Create a desktop landing page wireframe, a simple layout made of grey boxes, with numbered markers and a matching specification panel. The result shows page structure alongside short implementation and user experience notes.

• Planning a desktop landing page layout
• Sharing implementation notes with developers
• Reviewing navigation, hero, and feature placement

Create a single HTML wireframe for a desktop dashboard using grey blocks, placeholder text bars, and crossed image boxes. Review navigation, information hierarchy, and panel placement before choosing final colors, copy, or imagery.

• Reviewing a desktop dashboard layout
• Checking navigation and information hierarchy
• Planning KPI, chart, and table placement

Choose an approach for map animations in Remotion, from satellite image overlays to globe views and terrain flythroughs. The skill compares five techniques and directs the agent to the implementation guide for the intended shot.

• Animate overlays on a static satellite image
• Create globe views or show 3D buildings
• Choose a map approach without an API key

Find biological pathways and functions enriched in gene lists or ranked gene data. Choose an appropriate statistical method, align gene identifiers, define comparison backgrounds, and turn results into interpretable tables and plots with multiple-testing corrections.

• Finding enriched pathways among differentially expressed genes
• Running GSEA on a complete ranked gene list
• Calculating gene-set scores for individual samples or cells

Create scientific charts in Python with detailed control over axes, colors, labels, legends, and layout. Arrange multiple plots in one figure and export PNG, PDF, or SVG files for papers and presentations.

• Create charts from experimental data
• Arrange multiple plots in one figure
• Adjust colors, labels, and annotations