Results for “detection”

Performs structure-based virtual screening using AutoDock Vina, SMINA, GNINA (CNN scoring), and DiffDock-L hybrid workflows with explicit choice rules across rigid vs flexible docking, cross-docking vs self-docking, binding-site detection (P2Rank, fpocket), receptor preparation (PDB2PQR, PROPKA), ligand preparation (meeko, OpenBabel), and ultralarge-library screening (ZINC22, Enamine REAL). Use when screening chemical libraries against a protein target to find candidate binders, ranking docking poses, or selecting a docking workflow for a specific scenario.

The English summary is being prepared. Showing the original description.

Detect and adjust for horizontal pleiotropy in two-sample Mendelian randomization by distinguishing uncorrelated (UHP) from correlated (CHP) pleiotropy and choosing among Egger, MR-PRESSO, MR-RAPS, CAUSE, LHC-MR, LCV, MR-Clust, MR-Mix, and contamination-mixture methods. Use when validating an MR causal claim, running the STROBE-MR sensitivity battery, suspecting a shared heritable confounder, working under weak-instrument or polygenic-exposure regimes, or reconciling discordant estimates across robust methods.

The English summary is being prepared. Showing the original description.

Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

The English summary is being prepared. Showing the original description.

Predicts whether a DNA variant alters mRNA splicing using sequence-based deep-learning tools — SpliceAI (10kb context dilated CNN, clinical default), Pangolin (multi-tissue), MMSplice (modular per-region CNN with calibrated ΔPSI), SpliceTransformer/TrASPr (tissue-aware transformers), SpliceVault (empirical 300K-RNA lookup of likely mis-splicing outcomes), CADD-Splice (composite score). Applies the ClinGen SVI 2023 framework for ACMG/AMP variant interpretation (PVS1, PP3, BP4 evidence codes), HGVS splicing nomenclature (c.123+1G>A, c.123-3T>G, r.spl?), extended-window scoring for deep-intronic pseudoexons, tissue-specific predictions, branchpoint variant detection (BPHunter, LaBranchoR), and splice-switching ASO design. Use when interpreting splice impact of clinical variants, prioritizing VUS, identifying deep-intronic pathogenic variants, or designing ASOs.

The English summary is being prepared. Showing the original description.

Align protein structures using Foldseek 3Di, TM-align, US-align, DALI, or Foldmason for structural MSA. Predict, score, and superpose backbone coordinates when sequence identity is below the twilight zone or remote-homology detection is required. Use when sequence MSA fails (<25% identity), when the dark proteome is the target, when AlphaFoldDB / ESM Atlas search is needed, or when structural superposition is the goal.

The English summary is being prepared. Showing the original description.

Process and analyze single-cell ATAC-seq data with Signac, ArchR, SnapATAC2, or Cell Ranger ATAC. Use when handling 10X scATAC or 10X Multiome (paired RNA+ATAC) data, performing per-cell QC, choosing between ArchR/Signac/SnapATAC2 ecosystems, building per-cluster consensus peaksets, integrating with paired scRNA-seq, doublet detection (AMULET vs ArchR vs scDblFinder), or running pseudobulk differential accessibility per cluster.

The English summary is being prepared. Showing the original description.

Use this skill when the user asks about driver fatigue management — circadian rhythm impact on driving, recognizing fatigue, fatigue risk management programs (FRMS), night driving safety, microsleep recognition, drowsiness detection technology, and how HOS rules relate to actual fatigue (and why 11-hour driving is risky even when legal). Reference FMCSA Fatigue Management research.

The English summary is being prepared. Showing the original description.

Build face recognition systems with InsightFace, ArcFace, enrollment pipelines, HDBSCAN clustering, and privacy-compliant architecture. Activate on: face recognition, face enrollment, face clustering, identity verification, facial search. NOT for: general object detection (computer-vision-pipeline), emotion analysis (ai-engineer).

The English summary is being prepared. Showing the original description.

Use for sensemaking, frame revision, anomaly handling, questioning strategy, and ambiguity diagnosis with the Data/Frame model before action is chosen. NOT for deterministic classification, settled execution planning, or situations where the frame is already stable.

The English summary is being prepared. Showing the original description.

Validates agent outputs against expected schemas and quality criteria. Reports structural and content checks within the declared evidence scope. Activate on 'validate output', 'output validation', 'schema validation', 'check output', 'output quality'. NOT for confidence scoring (use dag-confidence-scorer) or hallucination detection (use dag-hallucination-detector).

The English summary is being prepared. Showing the original description.

Synthesizes evidence-linked feedback from validation results, confidence evidence, and iteration triggers. Proposes bounded revision guidance for a stated acceptance failure. Activate on 'synthesize feedback', 'improvement suggestions', 'actionable feedback', 'iteration guidance', 'feedback generation'. NOT for iteration detection (use dag-iteration-detector) or convergence tracking (use dag-convergence-monitor).

The English summary is being prepared. Showing the original description.

Analyze hierarchical DAG-like systems by separating undirected substrate from ordering metadata, then classifying diamonds, mixers, shortcuts, and hidden cycle structure. Use for workflow architecture, citation or genealogy DAG comparison, and information-flow diagnosis. NOT for ordinary cycle detection in arbitrary graphs, one-node runtime debugging, or systems with no real ordering constraint.

The English summary is being prepared. Showing the original description.

Analyze undirected cycle structure within a valid DAG using the source paper's orientation and contraction method. Use for cycle-basis analysis with explicit graph and reduction choices. NOT for directed feedback loops, workflow deadlocks, or runtime resilience claims.

The English summary is being prepared. Showing the original description.

Handle mental health crisis situations in AI coaching safely. Use when implementing crisis detection, safety protocols, emergency escalation, or suicide prevention features. Activates for crisis keywords, safety planning, hotline integration, and risk assessment.

The English summary is being prepared. Showing the original description.

Comprehensive SEO analysis for any website or business type. Full site audits, single-page analysis, technical SEO (crawlability, indexability, Core Web Vitals with INP), schema markup, content quality (E-E-A-T), image optimization, sitemap analysis, and GEO for AI Overviews/ChatGPT/Perplexity. Industry detection for SaaS, e-commerce, local, publishers, agencies. Triggers on: SEO, audit, schema, Core Web Vitals, sitemap, E-E-A-T, AI Overviews, GEO, technical SEO, content quality, page speed.

The English summary is being prepared. Showing the original description.

Backlink profile analysis: referring domains, anchor text distribution, toxic link detection, competitor gap analysis. Works with free APIs (Moz, Bing Webmaster, Common Crawl) and DataForSEO extension. Use when user says backlinks, link profile, referring domains, anchor text, toxic links, link gap, link building, disavow, or backlink audit.

The English summary is being prepared. Showing the original description.

The Shape System — the content_ir kind registry for structured content. Use when adding, activating, or rendering a kind/shape, writing `__kind` JSON, kind schemas or samples, XML-tag/fence detection surfaces, kind components, workflow node input_kind/output_kind, or binding agent output to a kind.

The English summary is being prepared. Showing the original description.

Three.js interaction - raycasting, controls, mouse/touch input, object selection. Use when handling user input, implementing click detection, adding camera controls, or creating interactive 3D experiences.

The English summary is being prepared. Showing the original description.

Gives coding agents temporal awareness by reading the session transcript the host already writes, so the agent stops guessing about time and stops repeating commands that just failed. Activates whenever an agent reasons about recency, elapsed time, retry windows, deploy cooldowns, memory or file staleness, idle detection, or any "when / how long ago / how long did that take" question. Required for autonomous, parallel, or long-running agents.

The English summary is being prepared. Showing the original description.

Expert writing for technical content, social media, and developer documentation. Use when asked to write tweets, LinkedIn posts, blog posts, READMEs, commit messages, feature announcements, or any written content. Optimized to avoid AI detection patterns.

The English summary is being prepared. Showing the original description.

Writing style guide for technical content, social media, blog posts, READMEs, git commits, and developer documentation. Optimized to avoid AI detection patterns. Use when writing any content beyond code.

The English summary is being prepared. Showing the original description.

PSR-1 and PSR-12 coding standards knowledge base for PHP 8.5 projects. Provides quick reference for basic coding standard and extended coding style with detection patterns, examples, and antipattern identification. Use for code style audits and compliance reviews.

The English summary is being prepared. Showing the original description.

SOLID principles knowledge base for PHP 8.5 projects. Provides quick reference for SRP, OCP, LSP, ISP, DIP with detection patterns, PHP examples, and antipattern identification. Use for architecture audits and code quality reviews.

The English summary is being prepared. Showing the original description.

[61] VALIDATE. Comprehensive code quality check combining ESLint, TypeScript compilation, and unused code detection. Runs full lint suite with detailed error reporting and fix suggestions. Use before commits, after major changes, or when ensuring code quality standards.

The English summary is being prepared. Showing the original description.