Results for “single”

Converts a double-precision Simulink system or subsystem to single precision using DataTypeWorkflow.Single (Fixed-Point Designer). The single conversion replaces all user-specified double-precision data types, as well as output data types that compile to double precision, with single-precision data types. Use this skill when converting Simulink systems to single precision, reducing memory usage of a Simulink system, optimizing for embedded targets. Do NOT use for standalone MATLAB .m code single conversion.

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Find and work with ENCODE single-cell genomics data including scRNA-seq and scATAC-seq. Use when the user asks about single-cell experiments, cell type resolution, clustering from ENCODE data, deconvolution of bulk signals using single-cell references, or comparing single-cell vs bulk profiles. Covers platform differences (10X Chromium, Smart-seq2, Drop-seq), quality limitations of single-cell data, multimodal integration (RNA+ATAC), and cross-study reproducibility concerns. Also use for cell type annotation, gene detection limits, dropout artifacts, and single-cell data structure in ENCODE.

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Check single-cell RNA-seq data quality, filter low-quality cells using median absolute deviation, a measure of spread, and compare plots before and after filtering. Outputs include filtered data and the original dataset with quality annotations.

• Assess single-cell RNA-seq data quality
• Filter low-quality cells and rarely detected genes
• Compare quality plots before and after filtering

Prepare single-cell or single-nucleus RNA-seq analysis by checking inputs and selecting workflows that turn FASTQ sequencing files into count matrices. Route quality checks, cell annotation, clustering, and UMAP visualization to the embedded downstream skill.

• Start single-cell RNA-seq analysis from FASTQ files
• Choose a workflow for generating count matrices
• Route existing matrices to quality checks and cell annotation

Use scGPT to convert single-cell gene expression data into numerical representations of cells and genes. These representations support clustering, data integration, cell-type annotation, and analyses of gene perturbations or regulatory relationships.

• Creating cell representations from AnnData
• Preparing single-cell data for clustering and integration
• Annotating cell types with or without fine-tuning

Analyze single-cell RNA-seq data with Scanpy, from quality checks and normalization to cell grouping, exploratory marker genes, and plots. Bundled scripts also prepare sample-level count summaries and support conversion of R objects to h5ad.

• Check data quality and normalize single-cell measurements
• Visualize cell groups with UMAP or t-SNE
• Explore marker genes and review cell-type labels

Analyze single-cell data with scvi-tools, using deep learning to combine datasets, correct differences between experiments, and transfer cell labels. Model guides and reusable scripts cover RNA, protein, chromatin accessibility, spatial data, and downstream analysis.

• Integrate single-cell RNA datasets and correct batch effects
• Analyze paired RNA and protein or RNA and ATAC measurements
• Transfer cell-type labels from reference data

Convert long Markdown specifications, reports, and plans into a single HTML document with a sticky table of contents, search, section tracking, and code-copy buttons. Apply previously configured brand colors and layout styles.

• Turn long specifications into navigable HTML documents
• Add search and a table of contents to reports
• Apply consistent brand styling to technical documents

Build or review quality control workflows for single-cell and single-nucleus RNA sequencing data. Select filters from observed data, detect doublets, assign cell types, and create UMAP plots that summarize cell relationships, while recording analysis decisions and outputs.

• Choosing quality filters from dataset distributions
• Detecting doublets and checking ambient RNA
• Assigning cell types using references or marker genes

Extract details from TikTok video URLs, including captions, author profiles, view and engagement counts, music, and hashtags. Process individual posts or a list of URLs, with image links available for slideshow posts.

• Check views and likes for a specific video
• Archive TikTok post metadata
• Collect details from multiple video URLs

Configure dependency injection in .NET MAUI, a way to supply objects with the services they need. Covers service registration, instance lifetimes, page and ViewModel constructors, Shell navigation, platform implementations, and test substitutions.

• Register services, Pages, and ViewModels
• Choose shared or freshly created service instances
• Connect Shell navigation with constructor injection

Predict structures of protein, nucleic acid, and small-molecule complexes with ESMFold2, using single sequences or aligned related sequences. The skill also covers ESMC models for numerical sequence representations, mutation scoring, and predictions of contacts between residues.

• Predicting complex structures from single sequences
• Assessing designed binders with ESMFold2-Fast
• Predicting structures using aligned related sequences

Configure Vite with vite-plugin-singlefile for mandatory single-file HTML bundling of MCP Apps. All assets (JS, CSS, images, fonts) must be inlined into a single HTML file for sandboxed iframe compatibility.

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Analyzes single-cell pooled CRISPR screens (Perturb-seq, CROP-seq, Perturb-CITE-seq, ECCITE-seq, multiome) where each cell carries an sgRNA and a scRNA-seq / surface-protein / chromatin readout. Covers experimental design (direct-capture Perturb-seq Dixit 2016 vs CROP-seq 3'UTR-barcoded Datlinger 2017 vs ECCITE-seq vs Multiome), MOI for sgRNA assignment, escaper-cell filtering (Mixscape, Papalexi 2021), SCEPTRE NB GLM + permutation for low-MOI (Barry 2024 Genome Biol 25:124), the Pertpy framework, factor decomposition, genome-scale Perturb-seq (Replogle 2022 Cell, 2.5M cells), and per-perturbation single-cell DE. Use when running a single-cell CRISPR screen, choosing direct-capture vs CROP-seq architecture, filtering escaper cells, performing single-cell DE, integrating Perturb-seq with pathway analysis, scaling to GW CRISPRi via Replogle protocol, or analyzing multi-omics screens.

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Cross-framework enhancement overlay for choosing a multi-agent topology BEFORE writing any agent. A binary-question rubric — is single-agent + tools enough? do agents need to know about each other? does the output need one voice? — maps the answer to single-agent / supervisor / swarm / sequential / hierarchical. Activates when a coder agent is tempted to "split the work into roles" or reaches for a multi-agent framework. Encodes the *selection rubric* that the per-framework skills assume but never surface. Search keywords: when to use multi-agent, single vs multi agent, do I need multiple agents, supervisor vs swarm, multi-agent vs single agent, agent team design.

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Nexscope Ozon product detail query: fetches the complete detail of a single Ozon product by SKU, returning title, price (RUB), rating, review count, QA count, total and daily average sales within the stats window, revenue, stock, category ranking, daily sales trend, brand, seller, fulfillment method (FBO/FBS/OZON), weight, and listing time/days/months. Use for single product deep analysis, competitor product teardown, Ozon product selection assessment, listing diagnosis, sales trend and category ranking tracking. Trigger when the user mentions Ozon product detail, Ozon single product analysis, Ozon SKU query, competitor product data, Ozon sales trend, Ozon category ranking, Ozon stock, Ozon listing time, Nexscope Ozon product search, Ozon product detail, Ozon SKU lookup, single product analysis, competitor product teardown, Ozon sales trend, category rank. Also trigger when the intent is to view detailed data of an Ozon product, even without explicitly mentioning Nexscope.

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Evaluates single-asset GP-led transactions with stand-alone asset valuation, financing structure, and rolling LP vs cashing out analysis. Use when analyzing single-asset CVs, evaluating trophy asset transactions, or structuring single-asset rolls.

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Guide for integrating CellxGene Census single-cell data with ENCODE bulk experiments. Use when users need cell-type-specific expression context for ENCODE regulatory data, want to deconvolve bulk ENCODE signals, or validate regulatory elements at single-cell resolution. Trigger on: CellxGene, single-cell atlas, cell type expression, Census, cell type specificity, single-cell context, scRNA-seq atlas.

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Analyzes single-cell pooled CRISPR screens (Perturb-seq, CROP-seq, Perturb-CITE-seq, ECCITE-seq, multiome) where each cell carries an sgRNA and a scRNA-seq / surface-protein / chromatin readout. Covers experimental design (direct-capture Perturb-seq Dixit 2016 vs CROP-seq 3'UTR-barcoded Datlinger 2017 vs ECCITE-seq vs Multiome), MOI for sgRNA assignment, escaper-cell filtering (Mixscape, Papalexi 2021), SCEPTRE NB GLM + permutation for low-MOI (Barry 2024 Genome Biol 25:124), the Pertpy framework, factor decomposition, genome-scale Perturb-seq (Replogle 2022 Cell, 2.5M cells), and per-perturbation single-cell DE. Use when running a single-cell CRISPR screen, choosing direct-capture vs CROP-seq architecture, filtering escaper cells, performing single-cell DE, integrating Perturb-seq with pathway analysis, scaling to GW CRISPRi via Replogle protocol, or analyzing multi-omics screens.

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Maps query single-cell data onto reference atlases and transfers cell-type labels using scArches surgery (scVI/scANVI), Symphony, Azimuth, CellTypist, scPoli, popV, and foundation models, with explicit out-of-distribution and label-transfer uncertainty. Use when annotating new single-cell datasets against a pre-trained reference, deciding which mapping method fits, or judging whether transferred labels are trustworthy. For de novo clustering and manual annotation see single-cell/cell-annotation; for batch integration without a reference see single-cell/batch-integration.

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Analyzes single-cell pooled CRISPR screens (Perturb-seq, CROP-seq, Perturb-CITE-seq, ECCITE-seq, multiome) where each cell carries an sgRNA and a scRNA-seq / surface-protein / chromatin readout. Covers experimental design (direct-capture Perturb-seq Dixit 2016 vs CROP-seq 3'UTR-barcoded Datlinger 2017 vs ECCITE-seq vs Multiome), MOI for sgRNA assignment, escaper-cell filtering (Mixscape, Papalexi 2021), SCEPTRE NB GLM + permutation for low-MOI (Barry 2024 Genome Biol 25:124), the Pertpy framework, factor decomposition, genome-scale Perturb-seq (Replogle 2022 Cell, 2.5M cells), and per-perturbation single-cell DE. Use when running a single-cell CRISPR screen, choosing direct-capture vs CROP-seq architecture, filtering escaper cells, performing single-cell DE, integrating Perturb-seq with pathway analysis, scaling to GW CRISPRi via Replogle protocol, or analyzing multi-omics screens.

The English summary is being prepared. Showing the original description.

Analyzes single-cell pooled CRISPR screens (Perturb-seq, CROP-seq, Perturb-CITE-seq, ECCITE-seq, multiome) where each cell carries an sgRNA and a scRNA-seq / surface-protein / chromatin readout. Covers experimental design (direct-capture Perturb-seq Dixit 2016 vs CROP-seq 3'UTR-barcoded Datlinger 2017 vs ECCITE-seq vs Multiome), MOI for sgRNA assignment, escaper-cell filtering (Mixscape, Papalexi 2021), SCEPTRE NB GLM + permutation for low-MOI (Barry 2024 Genome Biol 25:124), the Pertpy framework, factor decomposition, genome-scale Perturb-seq (Replogle 2022 Cell, 2.5M cells), and per-perturbation single-cell DE. Use when running a single-cell CRISPR screen, choosing direct-capture vs CROP-seq architecture, filtering escaper cells, performing single-cell DE, integrating Perturb-seq with pathway analysis, scaling to GW CRISPRi via Replogle protocol, or analyzing multi-omics screens.

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Build a trading analysis dashboard as a single HTML file, with dense market panels and interactive charts. Include light and dark themes, Live/Demo controls, chart focus views, and a keyboard command menu for navigating the interface.

• Prototyping a financial analysis terminal
• Presenting a dashboard with dense market panels
• Demonstrating chart tooltips and focus views

Create a paper-like one-page document for a white paper or studio introduction. The skill combines a warm parchment background, ink-blue accents, and language-specific serif fonts in a single HTML file, supporting English, Japanese, and Simplified Chinese.

• Create a studio introduction page
• Present a white paper as a single web page
• Build a Japanese one-pager with key metrics