Skip to content
cccskills
FreePublished on GitHub

bioconductor-clusterprofiler

This package supports functional characteristics of both coding and non-coding genomics data for thousands of species with up-to-date gene annotation. It provides a univeral interface for gene functional annotation from a variety of sources

The English summary is being prepared. Showing the original description.

See how to install

Files included (1)

  • SKILL.md4.0 KB

SKILL.md (original)

Review the instructions the agent will receive before you install.

clusterProfiler

Dependencies & Environment

Package-intrinsic requirements from the Bioconductor landing page — reproduce in any R environment.

  • Version: 4.20.0 · Bioconductor: 3.23 · R: ≥ 4.6
  • Imports: aisdk, AnnotationDbi, dplyr, enrichit, enrichplot, ggplot2, GO.db, GOSemSim, gson, httr, igraph, jsonlite, magrittr, plyr, qvalue, rlang, tidyr, yulab.utils
  • Install: BiocManager::install("clusterProfiler")

When to Use

  • Performing Over-Representation Analysis or Gene Set Enrichment Analysis.
  • Comparing biological themes among gene clusters.
  • Visualizing functional profiles of genomic coordinates (supported by ChIPseeker), genes, and gene clusters.
  • Querying Gene Ontology annotations online via AnnotationHub or KEGG Pathway and Module data.

When NOT to Use

  • For purely interactive web-based enrichment without an R environment (use web portals like DAVID directly).
  • When analyzing species not supported by online databases (unless providing customized user annotations).

Data Requirements

  • Genomic coordinates, gene lists, or gene clusters.
  • Annotations from supported ontologies/pathways (e.g., Disease Ontology, DisGeNET, Gene Ontology, KEGG, Reactome, Molecular Signatures Database) or customized user ontologies.

Key Parameters

  • No specific parameters are detailed in the provided vignette text.

Best Practices

  • Utilize the package's built-in visualization functions such as barplot, cnetplot, dotplot, emapplot, gseaplot, goplot, and upsetplot to interpret enrichment results.
  • When querying Gene Ontology, use AnnotationHub to support many species with online annotation queries.
  • Provide a reproducible example when posting bugs to the GitHub issue tracker.

Common Pitfalls

  • Failing to find answers to common problems because the user did not visit the clusterProfiler homepage documentation first.
  • Posting questions to the Bioconductor support site without tagging the post with clusterProfiler, leading to delayed responses.
  • Attempting to analyze unsupported species without supplying a customized ontology or user annotation.

Alternatives

  • DOSE: Specifically focused on Disease Ontology and Network of Cancer Gene enrichment.
  • ReactomePA: Specifically tailored for Reactome Pathway analysis.
  • goseq: Alternative for GO enrichment that explicitly corrects for RNA-seq transcript length bias.

Citations

  • G Yu, LG Wang, Y Han, QY He. clusterProfiler: an R package for comparing biological themes among gene clusters. OMICS: A Journal of Integrative Biology 2012, 16(5):284-287. doi: 10.1089/omi.2011.0118.

References

<!-- biomate-cta -->

Run this on BioMate

This skill is the knowledge layer — when, why, and how to use clusterprofiler. To run this analysis on your own data with managed compute, automated QC, and reproducible outputs, use BioMate — free to start.

▶ Open clusterprofiler on BioMate →

Reviews

No reviews yet. Tell others what you thought.

Comments

No login needed. Share how it worked for you, ask a question, or note where it did not run.

No comments yet. Be the first to write one.

Comments are published right away. Do not include personal information such as email addresses or phone numbers. Up to 2 URLs.

0 / 1,000 characters

This form is protected by reCAPTCHA, and the Google Privacy Policy and Terms of Service apply.

More skills from this repository

The package contains functions for exploratory oligonucleotide array analysis. The dependence on tkWidgets only concerns few convenience functions. 'affy' is fully functional without it.

The English summary is being prepared. Showing the original description.

bioMate-AI/biomate-bioconductor-kb797Updated Jun 21, 2026

This tool uses the label-tree function from HyPhy to annotate a phylogenetic tree. It allows users to select a subset of leaves using either a regular expression or a list of sequence names, and then apply a specified label to these selecte

The English summary is being prepared. Showing the original description.

bioMate-AI/biomate-bioconductor-kb797Updated Jun 21, 2026

This package provides a client for the Bioconductor AnnotationHub web resource. The AnnotationHub web resource provides a central location where genomic files (e.g., VCF, bed, wig) and other resources from standard locations (e.g., UCSC, En

The English summary is being prepared. Showing the original description.

bioMate-AI/biomate-bioconductor-kb797Updated Jun 21, 2026

apeglm provides Bayesian shrinkage estimators for effect sizes for a variety of GLM models, using approximation of the posterior for individual coefficients.

The English summary is being prepared. Showing the original description.

bioMate-AI/biomate-bioconductor-kb797Updated Jun 21, 2026

ASURAT is a software for single-cell data analysis. Using ASURAT, one can simultaneously perform unsupervised clustering and biological interpretation in terms of cell type, disease, biological process, and signaling pathway activity. Inputting a single-cell RNA-seq data and knowledge-based databases, such as Cell Ontology, Gene Ontology, KEGG, etc., ASURAT transforms gene expression tables into original multivariate tables, termed sign-by-sample matrices (SSMs).

The English summary is being prepared. Showing the original description.

bioMate-AI/biomate-bioconductor-kb797Updated Jun 21, 2026

AUCell allows to identify cells with active gene sets (e.g. signatures, gene modules...) in single-cell RNA-seq data. AUCell uses the "Area Under the Curve" (AUC) to calculate whether a critical subset of the input gene set is enriched with

The English summary is being prepared. Showing the original description.

bioMate-AI/biomate-bioconductor-kb797Updated Jun 21, 2026

See all skills by bioMate-AI

Report a problem with this skill