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bioconductor-miasim

Microbiome time series simulation with generalized Lotka-Volterra model, Self-Organized Instability (SOI), and other models. Hubbell's Neutral model is used to determine the abundance matrix. The resulting abundance matrix is applied to (Tree)SummarizedExperiment objects.

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miaSim

Workflows

Standard Workflow

library(miaSim)

# 1. Generate species interaction matrix
A_normal <- powerlawA(n_species = 4, alpha = 3)

# 2. Simulate time series using generalized Lotka-Volterra (gLV) model
tse_glv <- simulateGLV(
  n_species = 4, 
  A = A_normal, 
  t_start = 0, 
  t_store = 1000, 
  stochastic = FALSE, 
  norm = FALSE
)

# 3. Simulate community using Hubbell Neutral model
tse_hubbell <- simulateHubbell(
  n_species = 8, 
  M = 10, 
  carrying_capacity = 1000, 
  k_events = 50, 
  migration_p = 0.02, 
  t_end = 100
)

Input/Output Note: Inputs are ecological parameters (species count, interaction matrices, carrying capacity); outputs are simulated abundance matrices stored in TreeSummarizedExperiment objects.

When to Use

  • Simulating microbiome species abundance matrices and time series under varying ecological assumptions.
  • Modeling population dynamics using the generalized Lotka-Volterra model (simulateGLV) or its discrete version, the Ricker model (simulateRicker).
  • Simulating neutral community dynamics with migration, births, and deaths using Hubbell's Neutral model (simulateHubbell).
  • Generating species interaction matrices with specific distributional assumptions using powerlawA or randomA.

When NOT to Use

  • For fitting ecological models to real-world experimental data (use parameter estimation packages instead).
  • For simulating non-microbial networks that do not fit into the TreeSummarizedExperiment data container.

Data Requirements

  • Input parameters such as interaction matrices generated by powerlawA or randomA.
  • Outputs are structured as TreeSummarizedExperiment objects containing simulated abundance matrices.

Key Parameters

  • n_species: Number of species to include in the simulation or interaction matrix.
  • alpha: Power-law exponent parameter used in powerlawA.
  • diagonal: Diagonal values of the interaction matrix in randomA.
  • connectance: Connectance of the interaction matrix in randomA.
  • carrying_capacity: Carrying capacity of the community in simulateHubbell and simulateSOI.
  • migration_p: Migration probability in simulateHubbell and simulateHubbellRates.
  • t_end: End time of the simulation.
  • norm: Logical indicating whether to normalize the simulated abundances.

Best Practices

  • Ensure the number of species specified in the interaction matrix matches the number of species used in the simulation models.
  • Generate interaction matrices using powerlawA or randomA before running models like GLV, SOI, or Ricker.
  • Use randomE to generate production and consumption rate matrices specifically for the consumer-resource model (simulateConsumerResource).

Common Pitfalls

  • Specifying a different number of species in the interaction matrix than in the simulation model, which causes dimension mismatch errors. Ensure n_species matches the dimensions of A.

Alternatives

  • micodymora: A Python package for microbiome simulation.

Citations

  • Gao et al. 2023 (Case studies on microbiome simulation).

References

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