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Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.

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Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

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Writing style guide for technical content, social media, blog posts, READMEs, git commits, and developer documentation. Optimized to avoid AI detection patterns. Use when writing any content beyond code.

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入力画像を、カメラ・構図・人物配置を保った白黒ラフ絵コンテへ変換する。動画制作の構図資料を画像から作る場合に使う。

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docs (editable docs people share and comment on; the default for any document, named as a doc or not: a document, report, proposal, resume, cover letter, letter, contract, policy, form, template, worksheet, essay, handbook, guide, how-to, cheat sheet, SOP or other writing to keep, share, collaborate on, send, submit, print or sign; a doc exports to Word, PDF, Markdown or Google Docs, so needing a file to send, attach, upload, submit or print is no reason to pick Word, and a file nobody asked for is a doc, not Word; a plan, comparison, summary or notes asked in chat stays in chat; a pasted claude.ai artifact link may be a doc: check with docs tools first; Word or another file format named, tracked changes wanted, or a .docx to change or use as a template → that format's skill): making one → if no docs-connector instructions are in context, call its `guide` (topic.instructions) first; then create the doc (headings only, no body) before any search, file read or plan, even with files attached.

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This skill enforces Sentry's blog writing standards across every post — whether you're helping an engineer write their first blog post or a marketer draft a product announcement.

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Generate user-facing App Store release notes from git history since the last tag.

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Extract and update the EcoSIM output-variable catalog from Fortran history-field registration calls such as hist_addfld1d and hist_addfld2d. Use only when explicitly asked to update, regenerate, refresh, or rebuild the list of EcoSIM output variables from HistDataType.F90 or related F90 source; do not invoke for ordinary EcoSIM output analysis unless the variable list itself must be updated.

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Generate a "Journey Into [Project]" narrative report analyzing a project's entire development history from claude-mem's timeline. Use when asked for a timeline report, project history analysis, development journey, or full project report.

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An immersive, interactive, exhibit-style interface that blends storytelling, animation, and gamified elements to create a playful, experience-driven journey. The entire app sits on a single continuous brand-colored canvas (deep green)

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Narrative-driven design using visuals, copy, and interaction to guide users through engaging, emotionally resonant journeys.

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A playful, energetic, cartoonesque interface inspired by friendly children's-book illustrations — warm cream backgrounds, big bold custom display typography, saturated brand color blocks, thick black outlines, generously rounded shapes

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Monta peças verticais 9:16 — story, reels, shorts, TikTok — respeitando as áreas seguras da interface. Use quando o usuário disser "story", "reels", "shorts", "vertical", "9:16", "TikTok", "capa de reels", "template de story". A restrição dominante aqui é a UI do app, que cobre topo, base e lateral.

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Create, revise, or resolve applicability for SDD Composy user stories (US/SC) from an approved PRD and domain evidence, stopping at the human gate. Use after the PRD is approved — 'gerar as histórias de usuário', 'cenários de aceite', 'stories for prd-SLUG', 'is stories NOT_APPLICABLE here'. Do NOT use before the PRD is approved, for the TechSpec, or for user-story writing outside SDD.

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Monta peça única de feed — quote card, card de dado, capa, anúncio estático, imagem de link. Use quando o usuário disser "post", "card", "peça do feed", "imagem do post", "arte para o LinkedIn", "criativo estático", "quote card", "card de dado". Para sequência ver carousel-builder; para vertical ver story-reels.

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Inspect, rebuild, or verify the append-only SDD Composy semantic trace (events.jsonl) and its derived projection (trace.json). Use for 'o que aconteceu nessa task', 'histórico do fluxo', 'verificar o trace', 'rebuild the trace projection'. Do NOT use for a status overview (sdd-status), evidence manifests (sdd-evidence), or to edit trace history.

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Use when the user wants to evolve the features of a PRD roadmap with user stories — 'refinar as histórias da feature', 'escrever histórias de usuário', 'critérios de aceite', 'regras de negócio da feature', 'deixar a feature pronta', 'user stories for FT01' — one feature at a time, interviewing the human until each story meets the Definition of Ready: persona, value, INVEST, CA (Gherkin, happy and error paths) and RN from the PRD. Do NOT use to create the roadmap (prd-roadmap) or to change requirements without stories (prd-refine).

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Route supported legacy PWDEV Code command intent to canonical PWDEV Flow skills. Use when the user mentions old /pwdev-code:* commands, asks for PWDEV Code compatibility, or wants to adopt an existing .planning workspace without losing history.

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Inventory, archive, and summarize PWDEV Flow project history safely. Use when the user asks to find stale artifacts, archive completed work, clean the Flow workspace without deletion, or generate a changelog from verified history.

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Estrutura narrativa para copy — PAS, BAB, AIDA, PASTOR, Jornada do Herói e Golden Circle. Use quando o usuário disser "storytelling", "contar a história", "narrativa da marca", "o texto está sem fio condutor", "case de sucesso", ou quando a página precisar de narrativa em vez de argumento listado.

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Generate comprehensive changelogs from Git history, commit messages, pull requests, and issue tracking for release documentation. Use when preparing releases, documenting changes, creating release notes, categorizing commits, generating version summaries, maintaining project history, or automating changelog updates.

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Weekly engineering retrospective. Analyzes commit history, work patterns, and code quality metrics with persistent history and trend tracking. Team-aware with per-person contributions, praise, and growth areas. Use when asked for weekly retro, what shipped this week, or engineering retrospective.

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Weekly engineering retrospective. Analyzes commit history, work patterns, and code quality metrics with persistent history and trend tracking. Team-aware: breaks down per-person contributions with praise and growth areas. Use when asked to "weekly retro", "what did we ship", or "engineering retrospective". Proactively suggest at the end of a work week or sprint. (gstack)

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[BETA] Expert assistance for the Adobe I/O CLI plugin `@adobe/aio-cli-plugin-aem-rde` — the `aio aem rde` / `aio aem:rde` command tree used to deploy, inspect, log-tail, snapshot, and troubleshoot AEM Rapid Development Environments (RDEs). Activate ONLY when the user explicitly references RDE concepts: 'AEM RDE', 'Rapid Development Environment', `aio aem rde`, `aio aem:rde`, `aem-rde`, RDE snapshots, RDE deploy/install, `rde install`, `rde inspect`, `rde status`, `rde history`, `rde reset`, the `@adobe/aio-cli-plugin-aem-rde` package, or Cloud Manager program/environment configuration that is specifically for an RDE environment. Do NOT activate on generic AEMaaCS phrases like 'deploy to AEM Cloud', 'push my bundle', 'tail the publish log', 'cloud sandbox', or unqualified 'dispatcher-config / frontend / env-config deployments' — those belong to Cloud Manager pipelines, not RDE. This skill is in beta. Verify all outputs before applying them to production projects.

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