'normalization' 검색 결과

Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Query and download from NCBI Gene Expression Omnibus (GEO) and EMBL-EBI's BioStudies/ArrayExpress mirror. Use when finding expression datasets, navigating SuperSeries vs SubSeries, choosing between series-matrix (submitter-normalized) and raw supplementary files, downloading via GEOparse (Python) or GEOquery (R/Bioconductor), linking GEO to SRA for raw reads, or distinguishing GSE/GSM/GPL/GDS record types. Encodes the SuperSeries trap, the series-matrix normalization-trust caveat, GEOmetadb deprecation, ArrayExpress migration to BioStudies, and processed-vs-raw decision matrix.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Identifies super-enhancers from H3K27ac, MED1, or BRD4 ChIP-seq using ROSE, ROSE2, LILY, HOMER -style super, and ENCODE dELS cross-referencing. Handles peak stitching parameters, ranking choices, hockey-stick inflection, marker choice (H3K27ac vs MED1/BRD4), and cross-condition comparison with spike-in normalization. Constructs core regulatory circuitry (Saint-Andre 2016) from SE-encoded TFs. Use when identifying cell-identity / cancer-associated regulatory domains, comparing super-enhancers between conditions, identifying master transcription factor networks, or predicting BET-inhibitor responsiveness.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Visualizes ChIP-seq data using deepTools (computeMatrix, plotHeatmap, plotProfile, bamCoverage, bamCompare), pyGenomeTracks (modern INI-driven track plots), Gviz (R browser-style), EnrichedHeatmap (ComplexHeatmap-based), ChIPseeker tag heatmaps, and IGV batch screenshots. Handles bigWig normalization choices (CPM, BPM, RPGC, spike-in scaled), bamCompare operations (log2 ratio, subtract, SES), k-means clustering of heatmaps for biological subgrouping, and spike-in-scaled tracks for global-shift experiments. Use when generating publication-quality ChIP-seq signal heatmaps, profile plots, genome-browser tracks, or comparing samples visually.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Analyzes CUT&RUN (Skene Henikoff 2017) and CUT&Tag (Kaya-Okur 2019) chromatin profiling data. Handles SEACR vs MACS2 peak calling (with the btaf375 2025 benchmark guidance), pA-MNase vs pA-Tn5 vs pAG-Tn5 chimera differences, E. coli spike-in carryover normalization, IgG-only control logic (no input), characteristic fragment-size signatures (25-75 bp for CUT&Tag), and lower depth requirements (5M reads typical vs 25M for ChIP). Use when calling peaks from CUT&RUN/CUT&Tag, scaling by E. coli spike-in carryover, choosing SEACR norm mode, or comparing CUT&RUN/Tag results to traditional ChIP.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Standardizes molecular structures using ChEMBL chembl_structure_pipeline and RDKit rdMolStandardize covering sanitization, salt/solvent stripping, neutralization, tautomer canonicalization, stereochemistry standardization, mixture handling, and isotope normalization. Explicitly compares ChEMBL pipeline, canSARchem, and PubChem standardization choices. Use when preparing libraries for QSAR training, joining datasets across sources, deduplicating compound collections, or building canonical compound registries.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Quantifies alternative splicing as PSI (percent spliced in) from RNA-seq using rMATS-turbo (BAM-based event), SUPPA2 (TPM-based event), MAJIQ V3 (LSV-based Bayesian), leafcutter (annotation-free intron clusters), VAST-TOOLS (cross-species with microexon support), Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage), or IRFinder-S (intron retention coverage-aware). Distinguishes the five canonical event classes (SE, A5SS, A3SS, MXE, RI), special classes (microexons, exitrons, AFE/ALE), intron retention subtypes (canonical RI vs detained introns), and applies effective-length normalization. Use when measuring splice-site usage or isoform inclusion ratios from short-read RNA-seq.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Identify differentially accessible chromatin regions across conditions using DiffBind, csaw, DESeq2, or edgeR. Use when comparing ATAC-seq accessibility between treatment groups, choosing between consensus-peak vs sliding-window approaches, picking the correct normalization (full library vs reads-in-peaks), correcting batch with SVA/RUVseq, or interpreting log2FC and FDR thresholds in a chromatin context.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Generates Read Model/Projection for PHP 8.5. Creates optimized query models for CQRS read side with projections and denormalization. Includes unit tests.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Expert database architect specializing in data layer design from scratch, technology selection, schema modeling, and scalable database architectures. Masters SQL/NoSQL/TimeSeries database selection, normalization strategies, migration planning, and performance-first design. Handles both greenfield architectures and re-architecture of existing systems. Use PROACTIVELY for database architecture, technology selection, or data modeling decisions.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Builds automated threat intelligence feed integration pipelines connecting STIX/TAXII feeds, open-source threat intel, and commercial TI platforms into SIEM and security tools for real-time IOC matching and alerting. Use when SOC teams need to operationalize threat intelligence by automating feed ingestion, normalization, scoring, and distribution to detection systems.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Reference for producing Adobe Document Authoring (DA, da.live) and Edge Delivery Services (EDS, aka aem.live/Helix) compatible content. Use whenever generating HTML for DA upload, uploading media binaries to DA, publishing to aem.live, or driving the DA admin API (auth, source PUT, preview/publish). Covers block HTML format (canonical div-class form and accepted table alternate), section structure, page/section metadata, icons, links, images, default content, document skeleton constraints, block cell content normalization, the DA Source API contract, IMS auth, media storage, supported formats, Media Bus vs Content Bus delivery, and silent-failure rules that corrupt content.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Hunting skill for cache poison vulnerabilities. Built from 10 public bug bounty reports including X-Forwarded-Host poisoning, X-HTTP-Method-Override / GCS cache, reflected→stored XSS via cache, classic Omer-Gil Web Cache Deception, Cloudflare Cache Deception Armor bypass, session-token cache deception, Akamai hop-by-hop smuggling → server-side edge poisoning, and Kettle's 2024 path-normalization WCD against Cloudflare/Fastly/GCP. Host/X-Forwarded-Host injection that reaches app logic (reset-link poisoning, routing SSRF, OAuth issuer) is owned by hunt-host-header; this skill owns the case where the poisoned response is CACHED and served to other users. Use when hunting cache poisoning, Web Cache Deception, CDN-fronted apps.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.