3.3만건 · 관련도순
Detect transcription factor binding footprints in ATAC-seq using TOBIAS, HINT-ATAC, Wellington, or scprinter. Use when identifying bound TF sites within accessible regions, correcting Tn5 insertion bias before footprinting, choosing between cleavage-based and aggregate-based footprinters, or comparing differential TF activity between conditions.
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Process and analyze single-cell ATAC-seq data with Signac, ArchR, SnapATAC2, or Cell Ranger ATAC. Use when handling 10X scATAC or 10X Multiome (paired RNA+ATAC) data, performing per-cell QC, choosing between ArchR/Signac/SnapATAC2 ecosystems, building per-cluster consensus peaksets, integrating with paired scRNA-seq, doublet detection (AMULET vs ArchR vs scDblFinder), or running pseudobulk differential accessibility per cluster.
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Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.
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Audit required bioinformatics command-line tools against Lmod or Environment Modules on Linux HPC clusters, resolve executable and version requirements, and reproducibly deploy missing software through a site-approved EasyBuild, Spack, Bioconda/micromamba, Apptainer, binary, or source-build workflow. Use when Codex must inspect module availability, diagnose a missing or wrong-version bioinformatics tool, install scientific software on Slurm/PBS clusters, publish or validate modulefiles, or produce an auditable and rollback-ready cluster software deployment.
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Quantifies alternative splicing as PSI (percent spliced in) from RNA-seq using rMATS-turbo (BAM-based event), SUPPA2 (TPM-based event), MAJIQ V3 (LSV-based Bayesian), leafcutter (annotation-free intron clusters), VAST-TOOLS (cross-species with microexon support), Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage), or IRFinder-S (intron retention coverage-aware). Distinguishes the five canonical event classes (SE, A5SS, A3SS, MXE, RI), special classes (microexons, exitrons, AFE/ALE), intron retention subtypes (canonical RI vs detained introns), and applies effective-length normalization. Use when measuring splice-site usage or isoform inclusion ratios from short-read RNA-seq.
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Run build, deploy, inspect, and fix loops for .NET MAUI apps that already have MAUI DevFlow integrated. USE FOR: launching MAUI apps, selecting devices or emulators, waiting for or recovering agent connections, broker/port/adb connectivity issues, visual tree inspection, screenshots, UI interaction, Blazor WebView CDP debugging, reading DevFlow logs, and iterative app debugging. DO NOT USE FOR: first-time DevFlow package setup (use maui-devflow-onboard), or generic desktop automation unrelated to MAUI. INVOKES: maui devflow CLI, dotnet CLI, Android adb/android tools, and Apple simctl tools.
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Use when a driver or carrier asks about trucker mental health — depression, isolation, stress, fatigue, suicide risk, addiction, EAP programs, or wellness benefits. Covers evidence-based programs, hotlines, carrier-side EAP design, and how mental health affects safety and retention.
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Use this skill when the user asks about weigh-station bypass services — Drivewyze, PrePass, BestPass, NORPASS, OOIDA toll savings — how they work, eligibility criteria, costs, and how they tie into CSA scores. Reference each provider's coverage area + pricing.
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Use when a carrier asks which KPIs to track, how to build a fleet dashboard, what targets to set, cadence for reviewing metrics (daily / weekly / monthly / quarterly), and how to operationalize data into actions. Covers safety, operations, financial, driver, and customer KPIs.
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Use this skill when the user asks about trucking insurance — types of coverage (commercial auto, cargo, occupational accident, workers comp, bobtail/non-trucking, MCS-90 endorsement), federal minimums (49 CFR 387), what each covers, owner-operator vs company-driver implications, and how to read a Certificate of Insurance (COI). Cite 49 CFR 387.
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Use this skill when the user asks about CMV roadside inspection levels (Level I through Level VIII), what each level inspects, average inspection times, CVSA decal eligibility, what triggers a level upgrade, and how to coach drivers through a roadside inspection. Reference CVSA North American Standard Inspection Program.
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Use this skill when the user asks about refrigerated (reefer) trucking — cold chain compliance, FDA temperature recording requirements, Sanitary Transportation Rule (STR / 21 CFR 1.900-1.934), commodity-specific temperature ranges, refrigeration unit operation, temperature recorder calibration, and how a load is challenged if temperature deviates. Cite 21 CFR Part 1 Subpart Q.
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Use when a carrier asks how to spec a new tractor — engine choice (X15 vs DD16 vs PACCAR MX), transmission (manual vs automated manual vs automatic), axle ratio, sleeper size, fuel tanks, aerodynamic options, electronic features, warranty, fleet-spec vs premium-spec tradeoffs, and how spec choices affect resale value.
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Use this skill when the user asks how to build + manage direct shipper relationships — moving from spot market to dedicated lanes, customer scoring + credit assessment, contract terms, dedicated capacity agreements, KPI reporting, and how to handle customer disputes. Reference standard customer-carrier contract terms.
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Use this skill when the user asks about CMV oilfield operations — the 24-hour restart exemption (49 CFR 395.1(d)), oilfield-specific HOS rules, hot oil hauling, specialty equipment (winch trucks, kill trucks, frac sand haulers, water haulers), Texas Railroad Commission permitting, oilfield insurance specifics, and how oilfield operations differ from standard OTR trucking. Cite 49 CFR 395.1(d).
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Use this skill when the user asks about workers compensation for trucking — required by state law, classification of drivers, premium calculation (rate × experience modifier × payroll), Common Claim types in trucking, returning injured drivers to work, claims management, premium reduction strategies, and the difference between WC and Occupational Accident insurance for owner-operators. Reference state WC laws + IFTA / fleet insurance.
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Use when a carrier or driver asks about winter trucking, snow chain requirements, hot-weather precautions, summer tire failures, fuel additive use (anti-gel), idle management in extreme temperatures, weight restrictions in spring thaw, or how seasonal demand patterns affect freight rates and capacity.
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Use this skill to develop the capability to investigate crashes internally. Covers what to look for, when to escalate to outside experts, and documentation standards.
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Use this skill when the user asks about the financial differences between being an owner-operator vs company driver — gross pay, expenses, net pay, capital requirements, risk tolerance, lifestyle, and how to evaluate the path. Reference industry pay surveys.
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Use this skill to understand when electronic records are acceptable to FMCSA inspectors. Covers PDF, original-format, and search-ability requirements.
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Use this skill to understand the 6 levels of FMCSA roadside inspections and what each covers. Helps drivers know what to expect and helps carriers respond to inspection types.
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Use this skill when the user asks about Household Goods (HHG) movers — 49 CFR 375 + 376 consumer protection rules, FMCSA HHG-specific authority, mover certification, binding vs non-binding estimates, dispute resolution, weight ticket requirements, valuation coverage, and how HHG operations differ from general freight. Cite 49 CFR 375.
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Use this skill when the user asks about US-Mexico cross-border CMV operations — long-haul cross-border program, B-1 visas for Mexican drivers, FMCSA OP-1(MX) authority, US-Mexico border commercial zones, USMCA / NAFTA implications, customs brokerage (C-TPAT, FAST), and how a US carrier handles cross-border loads. Reference 49 CFR 365.501-507.
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Use this skill when a driver wants to use a CMV for personal travel after duty hours. Covers what qualifies as legitimate personal conveyance vs. work-related driving, FMCSA guidance on lodging/meals/home travel, and documentation requirements.
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