'r2' 검색 결과

Use when the user asks to "QA my conversion tracking before launch", "check my UTMs / pixel / event firing", "set up a tracking pre-flight", or "set the dedup rule so Meta and Google stop double-counting"; builds and fixes the measurement plumbing — conversion-event firing, UTM hygiene, cross-platform dedup rules, attribution-window alignment, and offline/iOS-ATT modeled-gap flags — as a pre-flight checklist plus a UTM/event-spec builder. Not for scoring R1/R2 — that is a scored veto in ad-account-auditor; not for account structure — use campaign-architect. 付费广告转化追踪QA/UTM规范/跨平台去重

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Provider (v6.x) state management for Flutter using ChangeNotifier and InheritedWidget with MVVM architecture pattern. Use this skill when implementing Provider-based state management, using Consumer/Consumer2 widgets for targeted rebuilds, accessing state with context.read/context.watch/context.select, setting up ChangeNotifierProvider or StateNotifierProvider, configuring MultiProvider for multiple providers, implementing ProxyProvider for dependent state, using Selector for optimized rebuilds, migrating from StatefulWidget to reactive state, maintaining legacy Provider codebases, debugging provider not found errors, preventing memory leaks with proper ChangeNotifier disposal, fixing context access issues in initState, or resolving .value constructor anti-patterns. Essential for apps with shared state across widgets (shopping carts, authentication, global settings, form state, theme management), when teaching state management fundamentals, or when debugging memory leaks and resource cleanup in Provider-based applications.

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Expert in Cloudflare Workers and the Edge Computing ecosystem. Covers Wrangler, KV, D1, Durable Objects, and R2 storage.

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Expert in Cloudflare Workers and the Edge Computing ecosystem. Covers Wrangler, KV, D1, Durable Objects, and R2 storage.

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Manual testing + bug filing for Enterprise / SaaS products. Parses a Feishu test doc (scope + repo + bitable URL), observes staging via claude-in-chrome, captures DOM/console/network/screenshot evidence, then dual-tracks each finding — Feishu Bitable record (with attachments) + GitHub issue (<REPO>#<ISSUE_NUMBER> format — English-primary, Chinese collapsed, R2-hosted screenshots, matching type label). Enforces dedup across bitable + GitHub issues + PRs, and mandatory P0/P1/P2 priority prompt when unclear. Trigger whenever the user says "test and report", "test and report side-by-side", "start system testing", "experience staging", "collaborate on bug filing", "collect feedback", a shared Feishu testing doc, a bitable URL + finding, or any UX/bug needing both bitable + GitHub tracking. Do NOT use for writing product code, PR review, CI debugging, or general "how do I use this product" questions — those go to normal dev / PR-review / support flows.

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Use Cloudflare KV, R2 and D1 from a Worker. Binding config, Worker code, cf commands, local vs remote data, D1 migrations. Use when adding or changing storage bindings or schemas.

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Look up current Cloudflare documentation (Workers, Pages, KV, R2, D1, Queues, DNS, WAF, platform APIs) through Cloudflare's official remote MCP server. Use when answering Cloudflare questions or writing Cloudflare config.

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Cloudflare-first platform selection. Decision trees for Workers, D1, R2, KV, DO, Queues, Vectorize, Containers, Sandboxes, Flagship, Agent Memory, Workflows v2. Default stack, override conditions, auth, data patterns, reliability.

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MANDATORY deploy after every code change. Typecheck → deploy → purge CDN → E2E on production → visual verify → fix-forward loop. Workers Builds native CI/CD, D1 Time Travel PIT recovery, D1→R2 long-term backups, wrangler rollback, wrangler secrets management, structured observability, cross-browser smoke tests, rollback procedures, and GitHub auto-configuration.

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Decision-grade statistical analysis for metabolomics intensity tables. Covers transformation and scaling (Pareto vs unit-variance as a hidden hypothesis), unsupervised structure (PCA/HCA for QC), permutation-validated PLS-DA/OPLS-DA (R2 vs Q2, double CV, VIP as heuristic), univariate testing (Welch/Mann-Whitney/ANOVA/LMM with covariate adjustment), and dependence-aware multiple testing. Use when testing which metabolites differ, building or validating a discriminant model, choosing a scaling, or correcting many correlated tests. For sample-wise normalization/drift correction see metabolomics/normalization-qc; for ML classifiers and selection-inside-CV leakage see machine-learning/biomarker-discovery and machine-learning/model-validation; for pathway interpretation see metabolomics/pathway-mapping; for design/power/multiplicity regime see experimental-design/multiple-testing.

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Predicts protein-coding gene structures (exons, introns, UTRs) in eukaryotic genomes with BRAKER3 (RNA-seq + protein evidence), BRAKER1/BRAKER2, GALBA (protein-only), Funannotate (fungi), GeMoMa (homology projection), or Helixer/Tiberius (deep-learning ab initio). Covers the evidence-first tool decision, mandatory soft-masking, the training-set-quality-dominates principle, OrthoDB clade-partition selection, the one-isoform-per-locus and missing-UTR traps, merge/split errors, and reference bias against orphan genes. Use when annotating a newly assembled eukaryotic genome, choosing a gene-prediction pipeline based on available evidence, or diagnosing a poor annotation.

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Discovers, classifies, and masks repetitive elements and transposable elements with RepeatModeler2 (de novo family library), RepeatMasker (masking against a library), EDTA (plant/structural TEs), or EarlGrey (auto-curating wrapper), and quantifies TE expression from RNA-seq with TEtranscripts/SQuIRE. Covers de-novo-library-as-curation-project, soft-vs-hard masking, the domesticated-gene over-masking massacre, Dfam-vs-RepBase, TE classification (Class I/II, family-vs-copy), Kimura repeat landscapes, LAI, and the RNA-seq multimapping problem. Use when masking repeats before gene prediction, building a TE library for a non-model genome, or analyzing transposable-element content or expression.

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Corrects the gene-independent copy-number artifact in CRISPR-Cas9 screens (Aguirre 2016 / Munoz 2016 Cancer Discov) where amplified loci appear essential from DNA-damage burden of simultaneous cuts. Covers the p53-dependent G2-arrest mechanism, CRISPRcleanR (Iorio 2018) unsupervised pre-hoc correction, CERES (Meyers 2017) joint CN + gene-effect model, Chronos (Dempster 2021) DepMap-standard population-dynamics + CN model with lowest residual bias, the decision tree by data availability, the Spearman LFC-vs-CN diagnostic, focal-amplification examples (ERBB2 in HER2+, MYC in colorectal, FGFR1 in head and neck), and CRISPRi/a alternatives that bypass the artifact. Use when screening cancer cell lines, diagnosing essentiality at amplified loci, choosing CRISPRcleanR / CERES / Chronos, deciding whether CN correction is needed before MAGeCK / BAGEL2 / drugZ, or switching from Cas9 to CRISPRi.

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Detects aberrant splicing in single rare-disease patients vs a control panel using FRASER 2.0 (Bioconductor; Beta-binomial autoencoder on Intron Jaccard Index, default delta cutoff 0.1, q hyperparameter), OUTRIDER (gene-level outlier expression via autoencoder denoising), LeafcutterMD (Dirichlet-multinomial outlier mode of LeafCutter for annotation-free junctions), and DROP (Snakemake pipeline integrating FRASER2 + OUTRIDER + monoallelic expression for clinical diagnostics). The statistical model is fundamentally different from differential splicing — single-sample-vs-cohort outlier detection rather than two-group comparison. Standard tool in EU rare-disease (Solve-RD) and NIH UDN programs. Use when applying RNA-seq to undiagnosed Mendelian disease, validating predicted splice variants in clinical samples, or detecting cryptic splicing in disease tissue.

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Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Predicts protein-coding gene structures (exons, introns, UTRs) in eukaryotic genomes with BRAKER3 (RNA-seq + protein evidence), BRAKER1/BRAKER2, GALBA (protein-only), Funannotate (fungi), GeMoMa (homology projection), or Helixer/Tiberius (deep-learning ab initio). Covers the evidence-first tool decision, mandatory soft-masking, the training-set-quality-dominates principle, OrthoDB clade-partition selection, the one-isoform-per-locus and missing-UTR traps, merge/split errors, and reference bias against orphan genes. Use when annotating a newly assembled eukaryotic genome, choosing a gene-prediction pipeline based on available evidence, or diagnosing a poor annotation.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Discovers, classifies, and masks repetitive elements and transposable elements with RepeatModeler2 (de novo family library), RepeatMasker (masking against a library), EDTA (plant/structural TEs), or EarlGrey (auto-curating wrapper), and quantifies TE expression from RNA-seq with TEtranscripts/SQuIRE. Covers de-novo-library-as-curation-project, soft-vs-hard masking, the domesticated-gene over-masking massacre, Dfam-vs-RepBase, TE classification (Class I/II, family-vs-copy), Kimura repeat landscapes, LAI, and the RNA-seq multimapping problem. Use when masking repeats before gene prediction, building a TE library for a non-model genome, or analyzing transposable-element content or expression.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Corrects the gene-independent copy-number artifact in CRISPR-Cas9 screens (Aguirre 2016 / Munoz 2016 Cancer Discov) where amplified loci appear essential from DNA-damage burden of simultaneous cuts. Covers the p53-dependent G2-arrest mechanism, CRISPRcleanR (Iorio 2018) unsupervised pre-hoc correction, CERES (Meyers 2017) joint CN + gene-effect model, Chronos (Dempster 2021) DepMap-standard population-dynamics + CN model with lowest residual bias, the decision tree by data availability, the Spearman LFC-vs-CN diagnostic, focal-amplification examples (ERBB2 in HER2+, MYC in colorectal, FGFR1 in head and neck), and CRISPRi/a alternatives that bypass the artifact. Use when screening cancer cell lines, diagnosing essentiality at amplified loci, choosing CRISPRcleanR / CERES / Chronos, deciding whether CN correction is needed before MAGeCK / BAGEL2 / drugZ, or switching from Cas9 to CRISPRi.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Detects aberrant splicing in single rare-disease patients vs a control panel using FRASER 2.0 (Bioconductor; Beta-binomial autoencoder on Intron Jaccard Index, default delta cutoff 0.1, q hyperparameter), OUTRIDER (gene-level outlier expression via autoencoder denoising), LeafcutterMD (Dirichlet-multinomial outlier mode of LeafCutter for annotation-free junctions), and DROP (Snakemake pipeline integrating FRASER2 + OUTRIDER + monoallelic expression for clinical diagnostics). The statistical model is fundamentally different from differential splicing — single-sample-vs-cohort outlier detection rather than two-group comparison. Standard tool in EU rare-disease (Solve-RD) and NIH UDN programs. Use when applying RNA-seq to undiagnosed Mendelian disease, validating predicted splice variants in clinical samples, or detecting cryptic splicing in disease tissue.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Comprehensive Cloudflare platform skill covering Workers, Pages, storage (KV, D1, R2), AI (Workers AI, Vectorize, Agents SDK), feature flags (Flagship), networking (Tunnel, Spectrum), security (WAF, DDoS), and infrastructure-as-code (Terraform, Pulumi). Use for any Cloudflare development task. Biases towards retrieval from Cloudflare docs over pre-trained knowledge.

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Generates PSR-20 Clock implementation for PHP 8.5. Creates ClockInterface implementations including SystemClock, FrozenClock, and OffsetClock for time abstraction and testing. Includes unit tests.

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12306-specific knowledge for booking train tickets via the Android app. Covers UC WebView virtual list behavior, proven booking flow, and common pitfalls. Uses appium-android-adb bridge OR direct uiautomator2 for all interaction.

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使用 wrangler CLI 操作 Cloudflare。当用户提到 Cloudflare、CF、Workers、Pages、R2、D1、KV、CDN、DNS、WAF 等时触发此技能。

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