's3' 검색 결과

End-to-end ATAC-seq workflow from FASTQ files to differential accessibility and TF footprinting. Covers alignment, peak calling with MACS3, QC metrics, and optional TOBIAS footprinting. Use when running end-to-end ATAC-seq analysis from FASTQ to differential accessibility.

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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.

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Calls ChIP-seq peaks with MACS3, MACS2, HOMER, or SPP across narrow (TF) and broad (histone) modes. Handles input control matching, fragment-size modeling vs --nomodel, effective genome size, ENCODE-style IDR vs naive overlap, hyper-ChIPable artifacts, and aligner-specific shifts. Use when calling peaks from ChIP-seq alignments, choosing between narrow vs broad mode for a histone mark, deciding model vs nomodel for low-depth data, applying ENCODE pseudoreplicate IDR, or reconciling MACS vs HOMER vs SPP results.

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Map nucleosome center positions, occupancy, and fuzziness from ATAC-seq fragment-size patterns using NucleoATAC, ATACseqQC, DANPOS3, or scprinter. Use when characterizing nucleosome organization at promoters and enhancers, calling +1/-1 nucleosomes flanking NFRs, generating V-plots for chromatin structure visualization, or comparing nucleosome positioning between conditions.

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Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.

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Provide comprehensive techniques for penetration testing AWS cloud enprojectnments. Covers IAM enumeration, privilege escalation, SSRF to metadata endpoint, S3 bucket exploitation, Lambda code extraction, and persistence techniques for red team operations.

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Analisa custo AWS e identifica desperdício em EC2, RDS, S3, EKS, EBS, Elastic IP, NAT Gateway e tráfego. Use quando o usuário disser "custo", "fatura", "está caro", "FinOps", "reduzir gasto AWS", "Cost Explorer", "otimizar custo". Quase todo leitura — seguro por natureza.

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Arquitetura e diagnóstico AWS — VPC, EC2, ECS, EKS, ALB, NLB, RDS, ElastiCache, S3, CloudFront, Route53, IAM, WAF, GuardDuty. Use quando o usuário disser "AWS", "VPC", "ALB", "RDS", "revisar a arquitetura", "Well-Architected", "subnet", "security group", "IAM", ou citar qualquer serviço AWS. Leitura livre; qualquer mudança passa pelo portão de confirmação.

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Backup, restore, snapshot, PITR, retenção e plano de recuperação de desastre — S3, Glacier, RDS, PBS, pg_dump, WAL. Use quando o usuário disser "backup", "restore", "restaurar", "snapshot", "PITR", "desastre", "DR", "RTO", "RPO", "perdemos dado".

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Provide comprehensive techniques for penetration testing AWS cloud environments. Covers IAM enumeration, privilege escalation, SSRF to metadata endpoint, S3 bucket exploitation, Lambda code extraction, and persistence techniques for red team operations.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Calls ChIP-seq peaks with MACS3, MACS2, HOMER, or SPP across narrow (TF) and broad (histone) modes. Handles input control matching, fragment-size modeling vs --nomodel, effective genome size, ENCODE-style IDR vs naive overlap, hyper-ChIPable artifacts, and aligner-specific shifts. Use when calling peaks from ChIP-seq alignments, choosing between narrow vs broad mode for a histone mark, deciding model vs nomodel for low-depth data, applying ENCODE pseudoreplicate IDR, or reconciling MACS vs HOMER vs SPP results.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Map nucleosome center positions, occupancy, and fuzziness from ATAC-seq fragment-size patterns using NucleoATAC, ATACseqQC, DANPOS3, or scprinter. Use when characterizing nucleosome organization at promoters and enhancers, calling +1/-1 nucleosomes flanking NFRs, generating V-plots for chromatin structure visualization, or comparing nucleosome positioning between conditions.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.

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Answers plain-word questions from a repo's CSV, JSON, Parquet and YAML files, or a .duckdb file, with DuckDB, and shows the SQL it ran. Read-only. Trigger to query, count, join, filter or summarise data files, including analytics CSVs exported from a video platform; to build a Parquet or DuckDB cache; to check no .duckdb, .wal or .parquet cache is committed or stale; when a query hits a locked database file; or say duckrunner (ask, cache, check, refresh). Needs the user-installed duckdb Python package; never a database server. DuckDB docs, S3 and MotherDuck are the duckdb-skills plugin's; outside research researchscribe's.

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Provide comprehensive techniques for penetration testing AWS cloud environments. Covers IAM enumeration, privilege escalation, SSRF to metadata endpoint, S3 bucket exploitation, Lambda code extraction, and persistence techniques for red team operations.

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Detects and prevents code injection attacks targeting serverless functions (AWS Lambda, Azure Functions, Google Cloud Functions) through event source poisoning, malicious layer injection, runtime command execution, and IAM privilege escalation via function modification. The analyst combines static analysis of function code, CloudTrail event correlation, runtime behavior monitoring, and IAM policy auditing to identify injection vectors across the expanded serverless attack surface including API Gateway, S3, SQS, DynamoDB Streams, and CloudWatch event triggers. Activates for requests involving Lambda security assessment, serverless injection detection, function event poisoning analysis, or serverless privilege escalation investigation.

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This skill teaches security teams how to deploy and operationalize Amazon GuardDuty for continuous threat detection across AWS accounts and workloads. It covers enabling protection plans for S3, EKS, EC2 runtime monitoring, and Lambda, interpreting finding severity levels, and building automated response workflows using EventBridge and Lambda.

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Detect abnormal access patterns in AWS S3, GCS, and Azure Blob Storage by analyzing CloudTrail Data Events, GCS audit logs, and Azure Storage Analytics. Identifies after-hours bulk downloads, access from new IP addresses, unusual API calls (GetObject spikes), and potential data exfiltration using statistical baselines and time-series anomaly detection.

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Hunting skill for subdomain takeover vulnerabilities. Includes modern provider fingerprints — Microsoft Azure DevOps `cloudapp.azure.com` regional-pool re-issue (1-click OAuth ATO via wildcard `reply_to`, Binary Security), Zendesk help-desk takeover → email interception → password reset chain (0xprial writeup), Vercel `cname.vercel-dns.com` deleted-project takeover, plus general Fastly CDN service re-attach and S3 dangling-bucket cookie-scope techniques. Use when hunting subdomain takeover — emphasis on ATO-chain primitives (OAuth `redirect_uri`, cookie-domain, email DNS).

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Complete bug bounty workflow — recon (subdomain enumeration, asset discovery, fingerprinting, HackerOne scope, source code audit), pre-hunt learning (disclosed reports, tech stack research, mind maps, threat modeling), vulnerability hunting (IDOR, SSRF, XSS, auth bypass, CSRF, race conditions, SQLi, XXE, file upload, business logic, GraphQL, HTTP smuggling, cache poisoning, OAuth, timing side-channels, OIDC, SSTI, subdomain takeover, cloud misconfig, ATO chains, agentic AI), LLM/AI security testing (chatbot IDOR, prompt injection, indirect injection, ASCII smuggling, exfil channels, RCE via code tools, system prompt extraction, ASI01-ASI10), A-to-B bug chaining (IDOR→auth bypass, SSRF→cloud metadata, XSS→ATO, open redirect→OAuth theft, S3→bundle→secret→OAuth), bypass tables (SSRF IP bypass, open redirect bypass, file upload bypass), language-specific grep (JS prototype pollution, Python pickle, PHP type juggling, Go template.HTML, Ruby YAML.load, Rust unwrap), and reporting (7-Question Gate, 4 validation ...

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Dense methodology covering DNS exfiltration (dnscat2, iodine, dns2tcp), HTTPS tunneling (domain fronting, CDN abuse, legitimate service channels), ICMP tunneling (icmpsh, ptunnel-ng), cloud storage dead drops (S3 presigned URLs, Azure Blob SAS tokens, GCS signed URLs), email-based exfil (SMTP, EWS, draft method), steganography (image, audio, document metadata), encoding/encryption (base64 chunking, XOR, AES), covert channels (custom protocol tunneling, HTTP header encoding, timing channels), and data staging (compression, splitting, encryption). Tools: dnscat2, iodine, dns2tcp, PacketWhisper, chisel, stunnel, icmpsh, ptunnel-ng, steghide, zsteg, OpenStego. MITRE ATT&CK: T1048 (Exfiltration Over Alternative Protocol), T1041 (Exfiltration Over C2 Channel), T1567 (Exfiltration Over Web Service), T1029 (Scheduled Transfer), T1030 (Data Transfer Size Limits), T1132 (Data Encoding), T1001 (Data Obfuscation). Use when planning or executing data exfiltration during authorized red team engagements or post-exploita...

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curl -s 'https://TARGET-NAME.s3.amazonaws.com/?max-keys=10'

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Cloud security attack methodology covering AWS, Azure, and GCP. Includes credential harvesting (IMDS, ~/.aws, env vars, leaked CI secrets, instance roles), enumeration with cloud-specific tools (pacu, ScoutSuite, Prowler, ROADtools, gcp_enum), privilege escalation paths (IAM PassRole, AssumeRole chains, Lambda/Functions privilege flips, Azure Owner-on-self, GCP serviceAccountTokenCreator), persistence techniques (IAM user/key creation, AAD app registration, GCP svc account key creation, EventBridge/Logic Apps backdoors), data exfiltration (S3/Blob/GCS, snapshot share, RDS/CosmosDB/Cloud SQL exfil), cloud-native lateral movement (cross-account assume, Azure AD multi-tenant, GCP project hierarchy), serverless attacks (Lambda env vars, layer hijack, Step Functions), Kubernetes-on-cloud (EKS/AKS/GKE-specific paths to node and AWS metadata), and CSPM evasion (CloudTrail blind spots, GuardDuty mute, Sentinel rule shaping). Use when the engagement scope is cloud accounts, when you've stolen cloud credentials, or...

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