702건 · 관련도순
Read, write, and convert multiple sequence alignment files using Biopython Bio.AlignIO. Supports Clustal, PHYLIP, Stockholm, FASTA, Nexus, and other alignment formats for phylogenetics and conservation analysis. Use when reading, writing, or converting alignment file formats.
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Parse and analyze multiple sequence alignments using Biopython. Extract sequences, identify conserved regions, analyze gaps, work with annotations, and manipulate alignment data for downstream analysis. Use when parsing or manipulating multiple sequence alignments.
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Simple Preference Optimization for LLM alignment. Reference-free alternative to DPO with better performance (+6.4 points on AlpacaEval 2.0). No reference model needed, more efficient than DPO. Use for preference alignment when want simpler, faster training than DPO/PPO.
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Helps build and review NeuroKit2 research workflows for physiological recordings such as ECG, respiration, and skin conductance. Covers preprocessing, time alignment, event analysis, heart rate variability, and complexity measures, while recording methods and checking data quality.
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Convert single-plane, single-channel two-photon TIFF images and timestamped behavioral position CSV files into NWB, a neuroscience data format. Preserve metadata, check clock alignment against acquisition evidence, and report format validation findings and source-to-output comparisons.
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Add or revise scene narration and speech-linked captions in the active iPolloWork video. Use configured voices, actual audio files, and measured alignment to adjust timing while preserving music, sound effects, and previous speech until replacement succeeds.
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Perform pairwise sequence alignment using Biopython Bio.Align.PairwiseAligner. Use when comparing two sequences, finding optimal alignments, scoring similarity, and identifying local or global matches between DNA, RNA, or protein sequences.
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Calculate alignment statistics including sequence identity, conservation scores, substitution matrices, and similarity metrics. Use when comparing alignment quality, measuring sequence divergence, and analyzing evolutionary patterns.
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Aligns learning material to the standard it claims — choosing the right framework for the grade and subject, citing standards by code rather than reproducing their text, and telling a real alignment from a decorative one. Use this to align a worksheet, lesson or workbook to Common Core, NGSS or a state framework, to check an alignment claim before publishing, or to decide what may lawfully be printed on the cover.
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Build whole-genome alignments using Progressive Cactus (Armstrong 2020 reference-free clade-level WGA), Minigraph-Cactus (Hickey 2024 pangenome-aware), LASTZ chain/net (UCSC pipeline), MUMmer4 (Marçais 2018 pairwise), minimap2 -x asm5/10/20 (Li 2018 fast pairwise), AnchorWave (Song 2022 WGD-aware), and Mauve / progressiveMauve (bacterial). Operates the HAL toolkit (Hickey 2013) for downstream extraction including halSynteny, halLiftover, halBranchMutations, and hal2maf. Use when constructing multi-species alignments for comparative-annotation projection (TOGA), synteny detection, conservation analyses (phyloP / PhastCons), or pangenome graph construction; selecting between reference-free (Cactus) and reference-anchored (LASTZ chains/nets) approaches; tuning sensitivity for closely vs distantly related genomes; or producing HAL files for genome-wide downstream tools.
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Calculate alignment statistics including sequence identity, conservation scores, substitution matrices, and similarity metrics. Use when comparing alignment quality, measuring sequence divergence, and analyzing evolutionary patterns.
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View, convert, and understand SAM/BAM/CRAM alignment files using samtools and pysam. Use when inspecting alignments, converting between formats, or understanding alignment file structure.
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Filter alignments by flags, mapping quality, and regions using samtools view and pysam. Use when extracting specific reads, removing low-quality alignments, or subsetting to target regions.
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Perform pairwise sequence alignment using Biopython Bio.Align.PairwiseAligner. Use when comparing two sequences, finding optimal alignments, scoring similarity, and identifying local or global matches between DNA, RNA, or protein sequences.
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Grounded in first principles, rigorously examine and refine a user's plan, task, decision, goal, strategy, proposal, or idea through structured, progressively deeper questioning, in order to bridge the gap between the User and the Agent. Use when the user explicitly requests grilling, challenge, pressure-testing, cross-examination, red-team review, pre-mortem analysis, or a decision audit. The goal is to uncover unclear objectives, hidden assumptions, contradictions, weak evidence, missing information, overlooked constraints, dependencies, risks, trade-offs, failure modes, and misalignment between intended outcomes and likely real-world results. Begin by establishing a shared understanding of the user's actual intent, goals, constraints, and success criteria. Ask focused, high-leverage questions rather than broad or repetitive ones. Adapt each question based on previous answers, probing deeper where uncertainty, unsupported assumptions, or strategic weaknesses remain. Regularly summarize the current understanding of the user's intent and position to confirm alignment, expose misunderstandings, and refine the problem or goal definition. Distinguish facts, assumptions, hypotheses, and unknowns. Challenge reasoning rigorously while remaining constructive, respectful, and solution-oriented. Continue until the reasoning is internally coherent, evidence-aware, constraint-conscious, risk-assessed, and translated into a clearer, more actionable goal, decision, or plan. Ultimately, achieving the super-alignment.
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Filter alignments by flags, mapping quality, and regions using samtools view and pysam. Use when extracting specific reads, removing low-quality alignments, or subsetting to target regions.
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Workflow for read alignment, sorting, indexing, mapping statistics, and downstream-ready alignment artifacts.
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Yoga practice with an emphasis on alignment, props, and staged progression as the Iyengar lineage teaches it, alongside enough context about the broader lineage landscape (Krishnamacharya's three students, modern Hatha, Ashtanga vinyasa, restorative, Yin, and chair yoga) that a routing agent can place a user correctly before giving instruction. Covers asana families, alignment heuristics, prop use, sequencing, and the non-negotiable injury-prevention rules. Use for any query about yoga postures, home practice design, teacher-training-level questions, or whether a given pose is safe for a given body.
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Build whole-genome alignments using Progressive Cactus (Armstrong 2020 reference-free clade-level WGA), Minigraph-Cactus (Hickey 2024 pangenome-aware), LASTZ chain/net (UCSC pipeline), MUMmer4 (Marçais 2018 pairwise), minimap2 -x asm5/10/20 (Li 2018 fast pairwise), AnchorWave (Song 2022 WGD-aware), and Mauve / progressiveMauve (bacterial). Operates the HAL toolkit (Hickey 2013) for downstream extraction including halSynteny, halLiftover, halBranchMutations, and hal2maf. Use when constructing multi-species alignments for comparative-annotation projection (TOGA), synteny detection, conservation analyses (phyloP / PhastCons), or pangenome graph construction; selecting between reference-free (Cactus) and reference-anchored (LASTZ chains/nets) approaches; tuning sensitivity for closely vs distantly related genomes; or producing HAL files for genome-wide downstream tools.
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Calculate alignment statistics including sequence identity, conservation scores, substitution matrices, and similarity metrics. Use when comparing alignment quality, measuring sequence divergence, and analyzing evolutionary patterns.
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Filter alignments by flags, mapping quality, and regions using samtools view and pysam. Use when extracting specific reads, removing low-quality alignments, or subsetting to target regions.
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Perform pairwise sequence alignment using Biopython Bio.Align.PairwiseAligner. Use when comparing two sequences, finding optimal alignments, scoring similarity, and identifying local or global matches between DNA, RNA, or protein sequences.
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Update docs, playbooks, shims, generated docs, and alignment checks so public instructions route to the right canonical sources.
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Build whole-genome alignments using Progressive Cactus (Armstrong 2020 reference-free clade-level WGA), Minigraph-Cactus (Hickey 2024 pangenome-aware), LASTZ chain/net (UCSC pipeline), MUMmer4 (Marçais 2018 pairwise), minimap2 -x asm5/10/20 (Li 2018 fast pairwise), AnchorWave (Song 2022 WGD-aware), and Mauve / progressiveMauve (bacterial). Operates the HAL toolkit (Hickey 2013) for downstream extraction including halSynteny, halLiftover, halBranchMutations, and hal2maf. Use when constructing multi-species alignments for comparative-annotation projection (TOGA), synteny detection, conservation analyses (phyloP / PhastCons), or pangenome graph construction; selecting between reference-free (Cactus) and reference-anchored (LASTZ chains/nets) approaches; tuning sensitivity for closely vs distantly related genomes; or producing HAL files for genome-wide downstream tools.
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