'rescue' 검색 결과

Turns Hi-C/Micro-C FASTQ into a deduplicated, filtered .pairs file with pairtools and decides whether the library worked. Covers the bwa mem -SP5M / bwa-mem2 / chromap --preset hic alignment idiom (mates mapped as independent single-end reads), pairtools parse vs parse2 and the walks-policy choice (5unique pairwise vs all for Pore-C/Micro-C concatemers), pair-type classification (keep UU and rescued UC), dedup (PCR vs optical/by-tile), select by pair_type/MAPQ/distance, restriction-fragment handling (restrict, Arima dual-enzyme, Micro-C/DNase fragment-free), and allele-specific phasing (pairtools phase to two coolers). The library-QC decision uses % long-range cis as the one-number quality metric, trans as the noise floor, orientation balance as fragment-map-free dangling-end/self-circle QC, and % duplicates as a complexity proxy. Use when processing Hi-C/Micro-C/Omni-C reads into pairs, judging library quality, handling multi-enzyme or restriction-agnostic protocols, or generating allele-specific contacts.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Comprehensive quality control for CLIP-seq libraries (eCLIP, iCLIP, iCLIP2, PAR-CLIP) covering library complexity (preseq), FRiP, IDR replicate reproducibility, read-distribution metagene, SMInput vs IgG control rationale, rRNA / snoRNA contamination, fragment-length distribution, and ENCODE-compliance thresholds. Use when assessing whether a CLIP library passed, deciding lenient vs stringent peak thresholds, comparing replicates with IDR rescue and self-consistency ratios, or distinguishing failed IP from over-amplified library.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Nat Commun 12:1302), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Triage an outage, loss of stakeholder trust or a failing engagement direction. Use for urgent recovery; distinguish diagnosis from authorized production mitigation.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

CEO/founder-mode plan review. Four modes: scope expansion, selective expansion, hold scope, scope reduction. 10 review sections from architecture through long-term trajectory. Nuclear scope challenge, dream state mapping, error and rescue registry. Optional cross-model outside voice.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Detect and recover text that was accidentally typed with the Taiwanese Bopomofo / Zhuyin (注音) keyboard while the input method (IME) was stuck in English/ASCII mode — what Taiwanese users call 注音亂碼 or 注音文 — producing meaningless-looking letter+digit sequences such as "su3cl3a8", "cl3", "g4", "2k7", "vu,4", or such garbled runs embedded in an otherwise normal request (e.g. "help me fix su3cl3a8", "看看這個 PR qk4ru.6", "幫我改 su3cl3a8xk7"). Use this skill whenever a user message contains short ASCII tokens that look like keyboard gibberish, do not form real English words, and cannot be understood normally — especially tokens with digits interspersed among letters (tone keys 3/4/6/7 or vowel keys 8/9/0), which are a strong sign of Mandarin phonetic keystrokes typed in the wrong input mode. Run scripts/decode_bopomofo.py to deterministically map the keystrokes back to 注音 symbols with a confidence score, then interpret the intended Traditional Chinese in context and continue the user's original request without making them retype. Do NOT trigger on genuine technical strings — code, file paths, URLs, git hashes, CLI flags, package names, version numbers — the script's exclusion rules and confidence tiers already filter these out. Especially valuable for Taiwanese developers who forget to switch input methods when prompting AI coding agents.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Create original short videos using the 威胁接力反转｜救援改写层级并重启追逐 Creative DNA for MiniMax H3 or Seedance 2.0. Use when the user wants this causal, camera, motion, rhythm, or payoff structure with new subjects and surfaces.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Create original short videos using the 多功能救援线｜跨裂隙送补给再撤离 Creative DNA for MiniMax H3 or Seedance 2.0. Use when the user wants this causal, camera, motion, rhythm, or payoff structure with new subjects and surfaces.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Think and work like an expert Molecular Biologist. Use when a task calls for Molecular Biologist judgment. Reasons from central-dogma sequence flow, binding affinity (Kd/Km/kcat), gene regulation, and biological-versus-technical replicate structure through MIQE-compliant RT-qPCR, ddPCR, Western/flow/microscopy, CRISPR editing with rescue, and IWGAV antibody validation while treating off-target reagent effects, batch effects, mycoplasma and cell-line misidentification, and toxicity-driven artifacts as first-class failure modes.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Think and work like an expert Cell Biologist. Use when a task calls for Cell Biologist judgment. Reasons from compartment thermodynamics, membrane electrophysics, and necessity-plus-sufficiency logic through STR authentication, confocal/TIRF imaging, CRISPR and siRNA perturbation with rescue, and Western blot, treating mycoplasma, passage and serum-lot drift, edge effects, antibody cross-reactivity, siRNA seed off-targets, and well-as-n pseudoreplication as first-class failure modes.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Comprehensive quality control for CLIP-seq libraries (eCLIP, iCLIP, iCLIP2, PAR-CLIP) covering library complexity (preseq), FRiP, IDR replicate reproducibility, read-distribution metagene, SMInput vs IgG control rationale, rRNA / snoRNA contamination, fragment-length distribution, and ENCODE-compliance thresholds. Use when assessing whether a CLIP library passed, deciding lenient vs stringent peak thresholds, comparing replicates with IDR rescue and self-consistency ratios, or distinguishing failed IP from over-amplified library.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Cell Reports 36:109597), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

End-to-end CLIP-seq pipeline from FASTQ to ENCODE-compliant binding sites, single-nucleotide crosslink maps, annotation, motifs, and (optionally) differential binding. Use when running the full Yeo lab eCLIP / iCLIP / iCLIP2 / iCLIP3 / irCLIP / PAR-CLIP analysis with SMInput control, protocol-specific UMI extraction, ENCODE STAR parameters, CLIPper or Skipper peak calling with stringent log2 FC and -log10 p thresholds, IDR rescue and self-consistency QC, and downstream motif registration with mCross or PEKA.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

CRE Capital Markets and Debt Maturity suite - 8 specialist skills for maturity diagnostics, refinance proceeds gaps, extensions, workouts, rescue capital, term sheet comparison, CMBS special servicing readiness, lender updates, and recap IC memos.

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Incremental refactorer. Use when refactoring ONE module at a time within a rescue workflow after safeguard has set up safety nets — never as a standalone refactor for greenfield code. Applies proven patterns: Strangler Fig, Branch by Abstraction, Expand-Migrate-Contract.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Act as a project-management copilot (PM/PMO) for the delivery loop: charters, plans and schedules, estimates, risk registers, status reports, scope changes, stakeholder communication, meeting actions, lessons learned, and portfolio roll-ups, in a per-project `_project/` workspace. Use whenever the user wants to start, plan, track, rescue, or report on a project: "kick off this project", "make a project plan / timeline / WBS", "what date can I commit to", "build a risk register", "run a pre-mortem", "write the weekly status report", "the deadline slipped, what now", "turn this meeting into action items", "review my project portfolio", in any language ("quản lý dự án", "gestion de projet", "プロジェクト管理", "项目管理"), even when they never say "project management". Also use whenever a `_project/` directory exists and the user asks about schedule, deadline, risk, status, scope, budget, or stakeholders. Not for what to build (product-manager) or company-level bets (strategy-board).

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Churn risk identification and intervention — scans health signals for at-risk accounts, classifies risk level (CRITICAL/HIGH/MEDIUM), and produces an intervention sequence per risk type. Use when asked to "find at-risk accounts", "who might churn", "build a churn prevention plan", "identify churn signals", or "rescue this account".

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Manage WezTerm mux servers for AI agent swarms on high-RAM servers (512GB+). Use when wezterm mux unresponsive, agent sessions need rescue, tuning scrollback, or configuring persistent remote sessions.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Foundation obedience training for dogs — sit, stay, come, heel, and down using positive reinforcement and marker training. Covers timing, reward hierarchy, session structure, distraction proofing, and common handler errors. Use when a new puppy (8+ weeks) is ready for foundation training, an adult dog lacks reliable basic commands, a rescue dog needs to learn the household's command vocabulary, before advancing to complex behaviors or off-leash work, or when existing commands have degraded and need re-establishing.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Delegate a bounded write-capable investigation or implementation task to Kimi through the companion runtime. Use only when explicitly invoked or when the user clearly asks to hand off a substantial fix to Kimi.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Use when the user says greenfield this or rescue this codebase, names a field (dark, red, blue, or brown), or diagnoses a subsystem. Not for specs: use to-spec. Not for remote or irreversible changes.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Diagnose and repair LaTeX build failures in local projects or supplied logs. Make minimal source fixes, preserve scientific content and reference keys, and report actual build evidence and unresolved errors.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.