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Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Resolve the architecture of focal oncogene amplifications — extrachromosomal DNA (ecDNA), breakage-fusion-bridge (BFB) cycles, homogeneously staining regions (HSR), and linear amplification — from whole-genome sequencing with AmpliconArchitect, the AmpliconSuite pipeline, and AmpliconClassifier. Covers copy-number seed selection, breakpoint-graph reconstruction, balanced-flow optimization, ecDNA classification, and the limits of depth-only amplification calls. Use when a focal amplification needs structural characterization, when distinguishing ecDNA from chromosomal amplification, suspecting ecDNA-driven oncogene amplification or therapy resistance, or selecting copy-number seeds for amplicon reconstruction.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Generates an app icon for macOS or iOS — a fast CoreGraphics placeholder and/or flat layered source art to finish in Icon Composer (the Liquid Glass / iOS 26+ standard). Produces appearance variants (light/dark/tinted) and installs into the asset catalog. Use when the user wants to create, generate, iterate, or update an app icon.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Workaround for Claude Code filtering BMP PUA Unicode (U+E000-U+F8FF). Supplementary PUA Nerd Font icons like 󰊤 󱃾 󰁹 (U+F0000+, e.g. nf-md-github, nf-md-kubernetes, nf-md-battery) can be written directly. BMP PUA icons (Powerline, Font Awesome, Devicons) require placeholder syntax like {{ U+E0A0 }} or {{ nf-fa-star }} (without spaces), which hooks auto-convert. Invoke when reading or writing Starship configs, tmux themes, shell prompts, or statuslines.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Generate web assets including favicons, app icons (PWA), and social media meta images (Open Graph) for Facebook, Twitter, WhatsApp, and LinkedIn. Use when users need icons, favicons, social sharing images, or Open Graph images from logos or text slogans. Handles image resizing, text-to-image generation, and provides proper HTML meta tags.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Resolve the architecture of focal oncogene amplifications — extrachromosomal DNA (ecDNA), breakage-fusion-bridge (BFB) cycles, homogeneously staining regions (HSR), and linear amplification — from whole-genome sequencing with AmpliconArchitect, the AmpliconSuite pipeline, and AmpliconClassifier. Covers copy-number seed selection, breakpoint-graph reconstruction, balanced-flow optimization, ecDNA classification, and the limits of depth-only amplification calls. Use when a focal amplification needs structural characterization, when distinguishing ecDNA from chromosomal amplification, suspecting ecDNA-driven oncogene amplification or therapy resistance, or selecting copy-number seeds for amplicon reconstruction.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Amplicon sequence variant (ASV) inference from 16S rRNA or ITS amplicon sequencing using DADA2. Covers quality filtering, error learning, denoising, and chimera removal. Use when processing demultiplexed amplicon FASTQ files to generate an ASV table for downstream analysis.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Run the viz pipeline to render icons from existing glyphs. Entry point for the viz subproject covering palette generation, data building, manifest creation, and icon rendering for skills, agents, and teams. Always use build.sh as the pipeline entry point — never call Rscript directly.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Detect missing glyphs, icons, and HD variants by comparing registries against glyph mapping files, icon directories, and manifests. Reports gaps for skills, agents, and teams across all palettes.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

为 OpenClaw 配置硅基流动 (SiliconFlow) 作为模型源。SiliconFlow 是国内领先的 AI 模型推理平台，提供 98+ 个 chat 模型，包含多个免费模型（Qwen3-8B、DeepSeek-R1-8B 等）。使用标准 OpenAI 协议（openai-completions）。包含 provider 注册、模型定义、别名配置、fallback 链接入和验证的完整流程。当管理员说想"加硅基流动"、"配 SiliconFlow"、"接入 SF 模型"、"加 Kimi"、"加 Qwen3"、"加免费模型"、"接入 DeepSeek V3.2"时使用此 skill。

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Resolve the architecture of focal oncogene amplifications — extrachromosomal DNA (ecDNA), breakage-fusion-bridge (BFB) cycles, homogeneously staining regions (HSR), and linear amplification — from whole-genome sequencing with AmpliconArchitect, the AmpliconSuite pipeline, and AmpliconClassifier. Covers copy-number seed selection, breakpoint-graph reconstruction, balanced-flow optimization, ecDNA classification, and the limits of depth-only amplification calls. Use when a focal amplification needs structural characterization, when distinguishing ecDNA from chromosomal amplification, suspecting ecDNA-driven oncogene amplification or therapy resistance, or selecting copy-number seeds for amplicon reconstruction.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Design engineering principles for making interfaces feel polished. Use when building UI components, reviewing frontend code, implementing animations, hover states, shadows, borders, typography, icons, micro-interactions, enter/exit animations, or any visual detail work. Supports quick and full review modes. Triggers on UI polish, design details, "make it feel better", "feels off", stagger animations, border radius, optical alignment, font smoothing, tabular numbers, image outlines, box shadows, icons, icon stroke weight, icon states, motion restraint.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

豆包 / 微信输入法个人词库：识别、导出、导入、备份；纠错优化； 搜索并生成行业/专业/定制词库（并入归档 industry-dict）。 不走官方导入页。搜索优先 argo，没有就走通用搜索兜底。本技能只出查询、抽词、改表、写回副本。 生态定位：直接操作豆包 / 微信输入法词库文件层（usr.dat / WeType LevelDB）的 agent skill， 文件层识别、批量导出、槽位感知注入、整表替换消错、行业词库造词后直接 apply； 格式互转工具和语音输入插件都不做词库管理。 触发：个人词库、词库导出、词库导入、反向注入、行业词库、专业词库、定制词库、 生成行业词库、做词库、行业术语、industry-dict、拼写纠正、豆包词库、 微信输入法词库、WeType、usr.dat、ime-lexicon、/ime-lexicon

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Resolve the architecture of focal oncogene amplifications — extrachromosomal DNA (ecDNA), breakage-fusion-bridge (BFB) cycles, homogeneously staining regions (HSR), and linear amplification — from whole-genome sequencing with AmpliconArchitect, the AmpliconSuite pipeline, and AmpliconClassifier. Covers copy-number seed selection, breakpoint-graph reconstruction, balanced-flow optimization, ecDNA classification, and the limits of depth-only amplification calls. Use when a focal amplification needs structural characterization, when distinguishing ecDNA from chromosomal amplification, suspecting ecDNA-driven oncogene amplification or therapy resistance, or selecting copy-number seeds for amplicon reconstruction.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Resolve the architecture of focal oncogene amplifications — extrachromosomal DNA (ecDNA), breakage-fusion-bridge (BFB) cycles, homogeneously staining regions (HSR), and linear amplification — from whole-genome sequencing with AmpliconArchitect, the AmpliconSuite pipeline, and AmpliconClassifier. Covers copy-number seed selection, breakpoint-graph reconstruction, balanced-flow optimization, ecDNA classification, and the limits of depth-only amplification calls. Use when a focal amplification needs structural characterization, when distinguishing ecDNA from chromosomal amplification, suspecting ecDNA-driven oncogene amplification or therapy resistance, or selecting copy-number seeds for amplicon reconstruction.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.

Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.

한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.