62건 · 관련도순
Build recommendation systems with collaborative filtering, matrix factorization, hybrid approaches. Use for product recommendations, personalization, or encountering cold start, sparsity, quality evaluation issues.
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All-in-one read preprocessing with fastp including adapter trimming, quality filtering, deduplication, base correction, and HTML report generation. Use when preprocessing Illumina data and wanting a single fast tool instead of separate Cutadapt, Trimmomatic, and FastQC steps.
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Preprocesses cell-free DNA sequencing data including adapter trimming, alignment optimized for short fragments, and UMI-aware duplicate removal using fgbio. Applies cfDNA-specific quality thresholds and fragment length filtering. Use when processing plasma cfDNA sequencing data before downstream analysis.
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Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller. Covers model selection, GPU acceleration, modified base detection, and quality filtering. Use when processing raw Nanopore data before alignment. Note: Guppy is deprecated; use Dorado for all new analyses.
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Amplicon sequence variant (ASV) inference from 16S rRNA or ITS amplicon sequencing using DADA2. Covers quality filtering, error learning, denoising, and chimera removal. Use when processing demultiplexed amplicon FASTQ files to generate an ASV table for downstream analysis.
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Birding by sight and sound — gestalt, song and call vocabulary, behavioral and habitat clues, and the discipline of producing eBird-grade records. Covers the vocabulary of bird field marks, the primary categories of vocalization, the habits of habitat-filtering, and the protocol for submitting records to a citizen-science database without degrading data quality. Use when the task is bird-specific observation or record-keeping.
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Audit, classify, and selectively forget stored memories. Covers memory enumeration and classification by type/age/access frequency, staleness detection for outdated references, fidelity checks using external anchors, a decision tree for selective withdrawal, tombstoned deaccession so removed content stays recoverable, counter-memory inoculation for failed strategies that would otherwise be re-derived, preemptive filtering rules for what should never become memories, and an audit trail so forgetting itself is reviewable. Use when memory has grown large and uncurated, when project state has shifted significantly since memories were written, when retrieval quality has degraded, or as periodic maintenance alongside manage-memory.
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Purify water from wild sources using boiling, filtration, and chemical methods. Covers source assessment and priority ranking, sediment pre-filtering, method selection (boiling, chemical, UV, filter), altitude-adjusted boiling procedure, chemical treatment dosages, and safe storage practices. Use when needing drinking water in a wilderness setting, when available water sources are of unknown quality, in an emergency survival situation where dehydration is a risk, or when making water safe for cooking or wound cleaning.
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Conduct rigorous cross-study meta-analysis of scRNA-seq data from ENCODE, integrating multiple single-cell transcriptomic datasets for a tissue/cell type. Use when the user wants to answer "what cell types exist in my tissue and what genes define them?" by combining scRNA-seq data across donors, labs, and platforms. Follows the Mawla et al. 2019 framework for assessing cross-study reproducibility, TIN-based quality filtering, and detection-limit-aware interpretation. Handles batch correction (Harmony/Seurat), dropout awareness, cross-contamination artifacts, and platform-specific biases. Use this skill for ANY scRNA-seq integration task, cross-dataset comparison, cell atlas construction, or reproducibility assessment involving ENCODE single-cell data.
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Calculates tumor mutational burden from WES/WGS/panel data with Friends of Cancer Research harmonization equations, per-assay calibration (FDA 10/Mb = 7.8 TSO500 = 8.4 OncomineTML), synonymous/indel/germline filtering, hypermutator tiering, blood TMB, and integration with HLA-LOH and neoantigen quality (Luksza 2017 fitness). Use when assessing ICI eligibility under tumor-specific cutoffs (McGrail 2021), comparing tissue vs bTMB, or auditing TMB-H reporting against ESMO 2024 and FDA pembrolizumab pan-tumor 2020.
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Curação de dados acelerada por GPU para treinamento de LLM. Suporta texto/imagem/vídeo/áudio. Recursos incluem deduplicação fuzzy (16× mais rápida), filtragem de qualidade (30+ heurísticas), deduplicação semântica, redação de PII, detecção NSFW. Escala em GPUs com RAPIDS. Use para preparar datasets de treinamento de alta qualidade, limpar dados web ou deduplicar grandes corpora.
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MUST USE when the user provides a photo ZIP, photo folder, or still-image event material and asks for photo selection, album selection, highlights, digest photos, curation, or quality filtering. Use this instead of analyze-footage when the source is primarily still photos and the requested work is selecting images rather than editing or video planning.
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Calculates tumor mutational burden from WES/WGS/panel data with Friends of Cancer Research harmonization equations, per-assay calibration (FDA 10/Mb = 7.8 TSO500 = 8.4 OncomineTML), synonymous/indel/germline filtering, hypermutator tiering, blood TMB, and integration with HLA-LOH and neoantigen quality (Luksza 2017 fitness). Use when assessing ICI eligibility under tumor-specific cutoffs (McGrail 2021), comparing tissue vs bTMB, or auditing TMB-H reporting against ESMO 2024 and FDA pembrolizumab pan-tumor 2020.
한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.
Calculates tumor mutational burden from WES/WGS/panel data with Friends of Cancer Research harmonization equations, per-assay calibration (FDA 10/Mb = 7.8 TSO500 = 8.4 OncomineTML), synonymous/indel/germline filtering, hypermutator tiering, blood TMB, and integration with HLA-LOH and neoantigen quality (Luksza 2017 fitness). Use when assessing ICI eligibility under tumor-specific cutoffs (McGrail 2021), comparing tissue vs bTMB, or auditing TMB-H reporting against ESMO 2024 and FDA pembrolizumab pan-tumor 2020.
한국어 요약은 준비 중입니다. 원문 설명을 표시하고 있습니다.