'dj' 검색 결과

Performs differential expression on bulk RNA-seq count data with DESeq2's negative-binomial GLM, Wald and LRT testing, apeglm/ashr/normal LFC shrinkage, independent filtering, Cook's outlier handling, VST/rlog transforms, and design formulas including paired, batch, and interaction terms. Use when running bulk DE, choosing DESeq2 over edgeR or limma-voom, building a paired or interaction design, applying LFC shrinkage for ranking or GSEA, choosing Wald vs LRT, troubleshooting padj=NA, picking VST vs rlog, importing salmon/kallisto via tximport, or analyzing prokaryotic RNA-seq.

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Add p-value brackets, significance asterisks, and effect-size annotations to distribution plots using ggpubr, ggsignif, and statannotations with correct test selection (parametric vs non-parametric vs paired), multiple-testing adjustment, and rendering of negative results. Use when a boxplot/violin/raincloud needs in-figure statistical comparisons between groups.

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Build volcano and MA plots from differential-expression / association results with LFC shrinkage, FDR-adjusted thresholds, sensible label placement, and axis-truncation conventions. Covers EnhancedVolcano, ggplot2, matplotlib, and the apeglm/ashr/normal shrinkage decision. Use when visualizing differential-expression results (RNA-seq, ChIP-seq, ATAC-seq, proteomics) or any per-feature effect-size + p-value table.

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Prepares statistical reports for clinical trials following CONSORT 2025, SPIRIT 2025, ICH E9(R1) estimands, and FDA 2023 covariate adjustment guidance. Covers Table 1 generation, analysis populations (ITT/FAS/PP/Safety), the 5 ICH E9(R1) intercurrent-event strategies, MMRM under MAR (mmrm), reference-based MI (rbmi J2R/CR/CIR), Permutt tipping-point sensitivity, and Rubin's-rules vs frequentist variance debate. Use when preparing regulatory submissions, defining estimands, or implementing missing-data sensitivity analyses.

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Detect and adjust for horizontal pleiotropy in two-sample Mendelian randomization by distinguishing uncorrelated (UHP) from correlated (CHP) pleiotropy and choosing among Egger, MR-PRESSO, MR-RAPS, CAUSE, LHC-MR, LCV, MR-Clust, MR-Mix, and contamination-mixture methods. Use when validating an MR causal claim, running the STROBE-MR sensitivity battery, suspecting a shared heritable confounder, working under weak-instrument or polygenic-exposure regimes, or reconciling discordant estimates across robust methods.

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Computes and interprets treatment effect measures (OR, RR, RD, HR, NNT) with calibrated confidence intervals (Wilson, Newcombe, Miettinen-Nurminen, MOVER, profile likelihood, Bender NNT) and reports marginal vs conditional estimands per FDA 2023 covariate adjustment guidance. Use when reporting treatment effects in confirmatory trials, comparing effect sizes across studies, or constructing forest plots.

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Performs logistic regression for clinical trial outcomes (binary, ordinal, multinomial) with marginal-vs-conditional estimand reporting per FDA 2023 covariate adjustment guidance, g-computation/standardisation for marginal effects, modified Poisson for RR, Brant test for proportional odds, Firth penalty for separation, and Hauck-Donner detection. Use when modeling binary or ordinal endpoints in confirmatory or exploratory clinical trials.

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Implements missing-data sensitivity analyses for confirmatory clinical trials including MMRM under MAR (with Kenward-Roger correction), reference-based multiple imputation (J2R, CR, CIR, LMCF per Carpenter-Roger 2013), Permutt delta-adjustment / tipping-point analysis, pattern-mixture identifying restrictions (CCMV, NCMV, ACMV), and the Cro vs Bartlett variance debate. Use when handling missing primary or secondary endpoint data in regulatory submissions following NRC 2010 and ICH E9(R1).

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Annotates ChIP-seq peaks to genomic features, nearest genes, ENCODE candidate cis-regulatory elements (cCREs), and regulatory domains. Uses ChIPseeker (R), HOMER annotatePeaks.pl (CLI), pyranges (Python), GREAT/rGREAT (regulatory domain gene-set enrichment), ChIP-Enrich (locus-length-adjusted), ENCODE SCREEN cCRE classification (PLS/pELS/dELS/CTCF-only/DNase-H3K4me3), and ENCODE-rE2G for cell-type-specific enhancer-gene linking. Handles nearest-TSS vs host-gene ambiguity, promoter window definition, and feature priority. Use when assigning genomic context to peaks, linking enhancer peaks to target genes, classifying peaks against ENCODE cCRE registry, or running gene-set enrichment on peak-associated genes.

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Use this skill when the user asks about CMV maintenance program requirements under 49 CFR 396.3 — what records must be kept, retention periods (12-18 months on vehicle file + 1 year after disposal), what counts as systematic inspection/repair/maintenance, preventive maintenance scheduling, brake adjustment records, and what auditors look for in vehicle files. Cite 49 CFR 396.3 + 396.21.

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Use this skill when the user asks about driver ergonomics — back/neck/shoulder injury prevention, seat adjustment + lumbar support, entering and exiting cab safely, load handling techniques, vibration reduction, stretching protocols, and how ergonomics affects workers compensation costs in trucking. Reference OSHA + ATA ergonomics research.

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Use this skill when the user asks about accident claims management — first-notice-of-loss procedures, working with insurance adjusters, claim severity classification, subrogation, claim closure timelines, and how to influence claim outcomes. Different from accident-investigation-playbook (which covers facts); this covers the insurance + claim side.

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Threat-models protocols where authority, scarce resources, bonds, bounties, escrow, reputation, ordering, adjudication, or settlement create strategic incentives. Use to test attack utility, conservation, collusion, Sybil resistance, oracle capture, griefing, custody, liquidity, and residual risk. NOT for choosing payment or bond amounts without evidence, smart-contract code audit, legal or employment classification, DeFi trading, admitting workers, or settling claims.

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Analyze a Coval accent testing report from runs across different speaker accents. Use when a user provides a Coval report URL, report export, run IDs, screenshots, or metric summary and wants evidence-backed next steps such as prompt changes, STT/confirmation adjustments, accent-robust routing, or expanded accent coverage.

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Analyze a Coval audio-quality testing report from runs across different voice, speaking-style, volume, interruption, and background-noise scenarios. Use when a user provides a Coval report URL, report export, run IDs, screenshots, or metric summary and wants evidence-backed next steps such as prompt changes, tool handling fixes, STT/TTS adjustments, trace setup, or expanded audio-scenario coverage.

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Redux state for agent execution and firing agent shortcuts. Use when editing features/agents/redux/, building agent UI, creating a conversation, touching assembleRequest or NDJSON stream state, wiring agent-state selectors, adding a per-conversation capability, or triggering a shortcut from a button, menu, or code (useShortcutTrigger, launchAgentExecution).

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ABP Framework cross-cutting patterns including authorization, background jobs, distributed events, multi-tenancy, and module configuration. Use when: (1) defining permissions, (2) creating background jobs, (3) publishing/handling distributed events, (4) configuring modules.

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Database migration best practices for schema changes, data migrations, rollbacks, and zero-downtime deployments across PostgreSQL, MySQL, and common ORMs (Prisma, Drizzle, Django, TypeORM, golang-migrate).

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Provides bidirectional translation (Chinese/English), grammar checking, and sentence polishing. Supports specific tense constraints, context-aware tone adjustment, and natural expression optimization. Outputs direct text without formatting.

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Complete color manipulation and green screen effects system. PROACTIVELY activate for: (1) Green screen/chromakey removal, (2) Color grading with LUTs, (3) Color correction (levels, curves, white balance), (4) Colorkey/color removal effects, (5) Hue/saturation manipulation, (6) Color space conversions (BT.709, BT.2020, HDR), (7) Color isolation effects, (8) Teal and orange look, (9) Vintage/film looks, (10) Color keying for transparency. Provides: chromakey and colorkey filters, LUT application (lut3d), curves and levels adjustment, color balance, selective color manipulation, color space handling, HDR tone mapping, professional color grading chains.

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A股技术分析工具包 — K线形态识别、技术指标计算、关键点位分析、资金流向解读。支持MACD/KDJ/RSI/均线系统/布林带，自动标注支撑位与阻力位，适合AI agent辅助操盘决策。

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You are a Python project architecture expert specializing in scaffolding production-ready Python applications. Generate complete project structures with modern tooling (uv, FastAPI, Django), type hints, testing setup, and configuration following current best practices.

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Complete guide for upgrading xUnit 2.9.x to 3.x. Use when upgrading xUnit v2 to v3 or understanding xUnit v3 new features and breaking changes. Covers package updates, async void fixes, IAsyncLifetime adjustments. Includes new features: Assert.Skip, Explicit Tests, Matrix Theory, Assembly Fixtures. Keywords: xunit upgrade, xunit v3, xunit 3.x, xunit migration, xunit upgrade, xunit.v3, OutputType Exe, IAsyncLifetime v3, Assert.Skip, SkipUnless, SkipWhen, Explicit attribute, MatrixTheoryData, AssemblyFixture, breaking changes

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Apply consistent photo adjustments across a set of images so they look like they were edited together. Use this skill whenever the user says "make my photos look cohesive", "give all these the same style", "apply a warm and golden feel to all of these", "make this cinematic", "match the look across my photos", "edit all my travel photos the same way", "batch edit these", "make these consistent", "fix my phone photos", or uploads a folder of photos and wants a unified, polished result. Also triggers for requests like "apply a preset to all of these", "make these look professional", or "they were shot in mixed lighting — can you fix them all". Outputs direct final image URLs plus an in-chat preview grid and optional Firefly Board link. Access: 🔐 Signed-In required | Gen AI: ❌

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