「asm」の検索結果

Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.

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Annotates bacterial and archaeal genomes (isolates, MAGs, plasmids) with Bakta (active versioned databases, NCBI-compliant output) or Prokka (legacy), producing GFF3/GenBank/EMBL/FASTA with INSDC locus tags. Covers Bakta-vs-Prokka-vs-PGAP-vs-DFAST choice, light-vs-full database tiers, translation-table selection (11/4/25), archaeal and leaderless-gene caveats, the small-ORF blind spot, pseudogene-vs-phase-variation, the pangenome re-annotation trap, and submission compliance. Use when annotating a newly assembled prokaryotic genome, choosing an annotation tool, re-annotating a collection for pangenomics, or preparing annotations for NCBI/DDBJ submission.

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Profiles a genome from raw reads BEFORE assembly with a k-mer spectrum (KMC or Jellyfish histogram), then models it with GenomeScope2 to estimate genome size, heterozygosity, repeat content, and ploidy, and Smudgeplot to infer ploidy from heterozygous k-mer pairs (diploid AB vs triploid AAB vs tetraploid AABB). Covers choosing k via Merqury best_k.sh, the k-mer-coverage vs sequencing-coverage confusion, reading het/repeat/contamination/organelle peaks, why noisy ONT must not be used for counting, and how the estimate becomes the NG50 denominator, the Flye -g value, the hifiasm --hom-cov/purge setting, and the 1.5-2x-too-big haplotig sanity check. Use when starting any de novo assembly, deciding whether short reads can work, estimating genome size for an unknown organism, diagnosing ploidy, or sanity-checking an assembly's size against expectation.

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Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.

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Build whole-genome alignments using Progressive Cactus (Armstrong 2020 reference-free clade-level WGA), Minigraph-Cactus (Hickey 2024 pangenome-aware), LASTZ chain/net (UCSC pipeline), MUMmer4 (Marçais 2018 pairwise), minimap2 -x asm5/10/20 (Li 2018 fast pairwise), AnchorWave (Song 2022 WGD-aware), and Mauve / progressiveMauve (bacterial). Operates the HAL toolkit (Hickey 2013) for downstream extraction including halSynteny, halLiftover, halBranchMutations, and hal2maf. Use when constructing multi-species alignments for comparative-annotation projection (TOGA), synteny detection, conservation analyses (phyloP / PhastCons), or pangenome graph construction; selecting between reference-free (Cactus) and reference-anchored (LASTZ chains/nets) approaches; tuning sensitivity for closely vs distantly related genomes; or producing HAL files for genome-wide downstream tools.

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Runs cis-pQTL Mendelian randomization for drug-target validation using UKB-PPP (Olink), deCODE (SomaScan), Fenland, INTERVAL, ARIC, and FinnGen-PPP proteomes plus colocalization triangulation, phenome-wide on-target adverse-effect scans, cross-platform Olink/SomaScan replication, and PAV (protein-altering variant) sensitivity. Use when nominating or de-risking a drug target from plasma-proteome GWAS, mimicking pharmacological inhibition via cis-pQTL instruments, separating shared-causal from LD-confounded signal under the Schmidt 2020 cis-MR framework, screening on-target adverse phenotypes pheWAS-style, or producing publication-grade STROBE-MR plus PP.H4 evidence for a target gene.

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Maxim food and beverage video — engineered for craving and order behavior via Sensory Anchoring + Mirror Neurons + ASMR Neural Pathway. Food videos that don't just look appetizing — they trigger physiological buying response.

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Adds a complete blog feature to an existing Blazor WebAssembly Static Web App with Azure Functions backend and Azure File Share for markdown storage. Use when implementing blog functionality in .NET Blazor WASM projects with Azure infrastructure. Includes post listing, detail pages, markdown rendering, Azure Storage integration.

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Deploys Uno Platform apps. Per-target guidance for WASM, iOS, Android, macOS, Windows, Linux.

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Tests Uno Platform apps. Playwright for WASM, platform-specific patterns, runtime heads.

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Hunt Microsoft SharePoint Server (2013/2016/2019/Subscription Edition) on-prem farms — anonymous endpoint enumeration, version disclosure, legacy SOAP login bypass (Authentication.asmx), ToolShell precondition chain (CVE-2025-53770), SafeControl reflection enumeration via Picker.aspx, NTLM Type-2 AD topology disclosure, custom-branding module discovery, EoL farm permanent-CVE-window exploitation, FormDigest anonymous issuance, file-extension blocklist NOT-an-oracle pattern, custom-zone Forms auth bridging on-prem AD. Use when target has SharePoint headers (SPRequestGuid, X-MS-InvokeApp, X-SharePointHealthScore, MicrosoftSharePointTeamServices) or paths (/_layouts/15/, /_vti_bin/, /_api/, /_catalogs/).

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Discover a single-page-app's hidden backend API from its public JS bundle, then test that API for broken access control / missing authentication. One of the highest-yield web plays in modern recon — SPAs ship their entire backend route map to the browser, and the API behind them is frequently missing the auth middleware the login page implies. Built from an authorized engagement where this play found an unauthenticated financial API that an ASM scan reporting hundreds of 'Criticals' completely missed. Use whenever a target serves a JS-heavy SPA (React/Vue/Angular/Next), an 'app'/'console'/'dashboard'/'portal' subdomain, or any `*api*` host shows up in recon. Leaked build artifacts (source maps / .env / .git / asset-manifest) are owned by hunt-source-leak; API version-inventory and behavioral diffing by hunt-shadow-api; this skill owns mapping a live SPA's backend routes from its JS bundle and testing them for broken access control / missing auth.

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Hunt ASP.NET-specific surface — ViewState deserialization (signed-only vs encrypted), machineKey recovery, dual-parser MAC-bypass anti-pattern, request-validator bypass, trace.axd/elmah.axd disclosure, load-balanced ViewState cross-node failures, SafeControl enumeration via reflection, customErrors mode=Off stack-trace leaks, classic Webforms .aspx/.asmx/.svc surface. Built for ASP.NET Webforms + WCF + SharePoint farms.

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Cryptographic security for agentic systems — zero-trust agent networking, signed message envelopes (JWS/JWE), capability-based security (ocaps), Merkle tree audit trails, WASM sandboxing, and formal verification. Covers CLI dev tool security, mTLS between agents, permission boundaries (least privilege for AI agents), and supply chain security for skills/plugins. Activate on: "agent security", "zero trust agents", "secure agent communication", "capability-based security", "ocap", "signed messages between agents", "agent audit trail", "sandbox agent execution", "agent permissions", "mTLS agents", "cryptographic verification", "agent supply chain", "OWASP agentic", "prove agent did X", "tamper-proof agent logs". NOT for: application-level SAST scanning (use security-auditor), network firewall rules (use infrastructure), SOC2/HIPAA compliance (organizational), or prompt injection defense (use prompt-engineer).

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