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Operate Gas City through its own doors: Mayor, doctor and native run state. Use when: Gas City is selected or a gc run looks stuck.

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Scaffold a minimal local Deep Agent in TypeScript by following the official quickstart, using provider-native web search instead of Tavily. Use when the user wants to quickly build or try a Deep Agent locally.

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Scaffold a minimal local Deep Agent in Python by following the official quickstart, using provider-native web search instead of Tavily. Use when the user wants to quickly build or try a Deep Agent locally.

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INVOKE THIS SKILL when using the langgraph CLI to scaffold, develop, build, or deploy LangGraph applications. Covers langgraph new, dev, build, up, deploy, and langgraph.json configuration.

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Inspect an agent repository and optional traces, interview the user, write reviewed Task Specs, build and audit Harbor tasks, and bootstrap reusable project World Knowledge Skills. Use for agent evals, benchmark design, Task generation, controlled Environments, synthetic data, Verifiers, Harbor runs, calibration, or continuous benchmark maintenance.

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INVOKE THIS SKILL when writing ANY LangGraph code. Covers StateGraph, state schemas, nodes, edges, Command, Send, invoke, streaming, and error handling.

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INVOKE THIS SKILL when your Deep Agent needs memory, persistence, or filesystem access. Covers StateBackend (ephemeral), StoreBackend (persistent), FilesystemMiddleware, and CompositeBackend for routing.

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Scaffold a minimal local LangGraph agent in Python by following the official quickstart. Use when the user wants to quickly build or try a LangGraph agent locally.

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INVOKE THIS SKILL when building, testing, or deploying Managed Deep Agents in LangSmith. Walks a user through their first agent end to end — interviewing them about what they want to build, mapping it onto what MDA can actually do, then scaffolding and deploying it. Covers the file-based project layout; define_deep_agent / defineDeepAgent; instructions, skills, memory, identity, tools, MCP servers, connections, middleware, sandboxes, schedules, channels, and evals; the mda CLI; GitHub deployments; and Context Hub.

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INVOKE THIS SKILL when building ANY Deep Agents application. Covers create_deep_agent(), harness architecture, SKILL.md format, and configuration options.

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INVOKE THIS SKILL when using subagents, task planning, or human approval in Deep Agents. Covers SubAgentMiddleware, TodoList for planning, and HITL interrupts.

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INVOKE THIS SKILL when implementing human-in-the-loop patterns, pausing for approval, or handling errors in LangGraph. Covers interrupt(), Command(resume=...), approval/validation workflows, and the 4-tier error handling strategy.

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INVOKE THIS SKILL when your LangGraph needs to persist state, remember conversations, travel through history, or configure subgraph checkpointer scoping. Covers checkpointers, thread_id, time travel, Store, and subgraph persistence modes.

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Scaffold a minimal local LangGraph agent in TypeScript by following the official quickstart. Use when the user wants to quickly build or try a LangGraph agent locally.

日本語の概要は準備中です。原文の説明を表示しています。

Dispatches many independent items in parallel: create a table, fan out to subagents, aggregate results. One row = one unit of work.

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Assemble a creator picture-in-picture product-listicle ad from a config — the creator stays FULL-FRAME the whole beat (voice plus lips generated together per beat, no separate VO, no cut to a full-frame product shot), and on each product beat three persistent overlays ride on top for the WHOLE beat — a title pill top-center, the DEMO in a rounded PiP window top-right (the brand's real UGC clip MUTED, or for a no-UGC brand the product's own autocropped UI still / screen-recording sized to fill the window), and a bottom product card (rounded thumbnail plus 'N · CATEGORY' small-caps plus product NAME in a serif face). Hook plus CTA beats are the creator full-frame with the title pill only. Assembly builds ONE full-1080x1920 transparent overlay PNG per beat, overlays it on the creator clip (cover-scaled to 1080x1920) for the whole beat keeping the native audio, concats all beats, then burns captions LAST as timed PIL PNG overlays (this ffmpeg has no libass) timed deterministically from the known per-beat script. This is the FREE deterministic assembly stage (overlay-PNG build plus cover-scale composite plus concat plus PIL-PNG caption burn); the creator anchor and the N native talking clips come from create-image-fal (Seedream v5 Pro) and create-video-fal (Seedance 2.0). Use for the creator-pip-listicle format.

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Generates, normalizes, and converts bedGraph signal tracks (4-column chrom/start/end/value, 0-based half-open) with bedtools genomecov, deepTools bamCoverage/bamCompare/bigwigCompare, bedtools unionbedg, and UCSC bedGraphToBigWig. Covers why a raw coverage bedGraph is not comparable across samples until normalized, the CPM/RPKM/BPM/RPGC normalization menu and the conserved-total assumption that makes them wrong under a global perturbation, the strict sorted-non-overlapping-chrom.sizes bedGraphToBigWig contract that silently corrupts a bigWig, effective-genome-size selection, and bin-size aliasing. Use when building or normalizing a coverage/signal track from a BAM, comparing tracks across samples or conditions, converting bedGraph to a browser-ready bigWig, or diagnosing a track that looks plausible but reports wrong heights.

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Extracts cfDNA fragmentomics features (DELFI genome-wide short/long ratios, WPS nucleosome positioning, Griffin GC-corrected accessibility profiles, end-motifs/MDS, OCF) for cancer detection and tissue-of-origin from plasma WGS. Centers on the nuclease-footprint reframe (every feature re-reads one nucleosome object), the mandatory GC correction, and the cross-protocol non-comparability that breaks naive classifiers. Runs FinaleToolkit (real CLI/Python, MIT) and the Griffin Snakemake pipeline; DELFI is a method, not a package. Use when deriving fragment-based signal from cfDNA, choosing a feature family for detection vs subtyping, or diagnosing why a fragmentomic model failed validation.

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Normalizes and transforms RNA-seq count matrices for DE, visualization, clustering, and ML. Covers between-sample (TMM, TMMwsp, RLE/median-of-ratios, upper quartile), within-sample (TPM, FPKM/RPKM), variance-stabilizing (VST, rlog, log-CPM), GC-content correction (cqn, EDASeq), and single-cell (scran deconvolution, scanpy normalize_total). Encodes the composition-bias rationale, the "most genes not DE" assumption and its catastrophic failure modes (MYC amplification, apoptosis, viral host shutoff, prokaryotic stress), the "lengthScaledTPM is not TPM" naming trap, the "TPM is not for DE" rule, the blind=TRUE vs FALSE decision, ERCC spike-in normalization (SBN), and the single-cell zero-inflation breakdown of TMM/RLE. Use when choosing or applying normalization, debugging shifted-MA-plot diagnostics, handling zero-heavy single-cell data, or correcting GC bias.

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Stores and operates on sparse expression matrices for single-cell and large bulk RNA-seq, covering dgCMatrix/dgRMatrix/dgTMatrix when-each-is-fast, the dgCMatrix (CSC, R) <-> CSR (Python) implicit transpose, AnnData (cells-rows) <-> SingleCellExperiment (cells-cols) orientation flip, HDF5/h5ad vs Zarr cloud-native shift, HDF5SummarizedExperiment + DelayedArray for out-of-memory bulk, scanpy backed mode for large h5ad, the ~10-15% density crossover where dense beats sparse, 10X format proliferation (MTX vs CellRanger H5 vs h5ad), the dense-conversion memory blow-up, and Dask + Zarr for consortium-scale matrices. Use when choosing sparse format, working with single-cell-sized matrices, importing/exporting 10X, debugging R/Python interop transposes, processing matrices too large for RAM, or building cloud-native pipelines.

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Build weighted gene co-expression networks to identify modules of co-regulated genes, relate them to phenotypes, and find hub genes using WGCNA, hdWGCNA, MEGENA, CEMiTool, and Gaussian graphical models. Covers signed-network choice, soft-threshold selection, module preservation, and the marginal-vs-partial-correlation distinction. Use when finding co-expression modules, identifying hub genes, relating gene networks to clinical or experimental traits, or building single-cell co-expression networks. For directed TF-target inference see scenic-regulons and grn-inference; for condition rewiring see differential-networks.

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Detect horizontal gene transfer (HGT / LGT) using compositional methods (GC%, codon usage, tetranucleotide z-scores via SIGI-HMM, AlienHunter, IslandViewer 4, IslandPath-DIMOB), phylogenetic-incongruence methods (AvP, HGTphyloDetect, ALE / GeneRax / AleRax reconciliation, RANGER-DTL), and BLAST-distribution methods (HGTector v2, DarkHorse, Alien Index). Use when screening prokaryote genomes for genomic islands and HGT events, distinguishing HGT from incomplete lineage sorting / differential gene loss / hybridization, mapping donor lineages via phylogenetic placement, separating eukaryotic HGT from contamination, ruling out gBGC as a false signal, or quantifying DTL rates with ALE/GeneRax on bacterial trees.

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Call copy number variants with the GATK best-practices workflows — the somatic CNV pipeline (CollectReadCounts, DenoiseReadCounts with tangent normalization, ModelSegments, CallCopyRatioSegments) and the germline GATK-gCNV pipeline (DetermineGermlineContigPloidy, GermlineCNVCaller cohort/case mode, PostprocessGermlineCNVCalls). Covers panel-of-normals construction, AnnotateIntervals/FilterIntervals, allelic-count integration, and QS-based filtering. Use when integrating CNV calling into a GATK variant pipeline, calling rare germline CNVs from an exome cohort, deciding between the somatic and germline GATK workflows, or diagnosing why tangent normalization removed a real event or why gCNV output has low precision.

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Bridges UIKit and SwiftUI with UIViewRepresentable, UIViewControllerRepresentable, UIHostingController, UIHostingConfiguration, coordinator delegates, and UIKit automatic observation tracking for shared @Observable state. Use when wrapping UIKit-only or third-party UIKit views/controllers in SwiftUI, embedding SwiftUI in UIKit, integrating mail/share/document/PDF/text-view surfaces, or migrating UIKit apps to SwiftUI incrementally.

日本語の概要は準備中です。原文の説明を表示しています。