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Classify constitutional (germline) copy number variants for clinical reporting using the 2019 ACMG/ClinGen technical standards points-based framework, with ClassifyCNV and AnnotSV for semi-automated scoring. Covers the separate copy-number-loss and copy-number-gain rubrics, the five-tier classification, ClinGen haploinsufficiency/triplosensitivity and dosage-sensitive regions, de novo and segregation evidence, and population-frequency benign evidence. Use when assigning pathogenic/likely-pathogenic/VUS/likely-benign/benign to a constitutional CNV, scoring a CNV against ACMG/ClinGen criteria, or distinguishing the automatable evidence from the case-specific evidence requiring manual input.

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Set up worker environment and per-worker state for parallel pools. Use when code needs paths, environment variables, database connections, loaded libraries, or expensive objects available on workers before parfor/parfeval runs. Teaches parallel.pool.Constant, parfevalOnAll, and parpool name-value pairs. Also use when refactoring existing code that uses spmd for side-effect setup (an anti-pattern). Triggers: worker setup, pool constant, per-worker state, non-serializable, loadlibrary on workers, database connection parfor, addpath workers, spmd before parfor, worker environment, reduce parfor overhead, parfor setup, resource creation in parallel loop, cannot serialize error, undefined function or variable on workers error, load data per worker, reduce data transfer, parallelize setup, improve parallel code.

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Inspects and configures the web application firewall (WAF) in front of a Power Pages production site. Lists the current state, recommends enabling protection when it is off, and walks the user through adding, updating, or removing custom rules — IP blocks, country blocks, path blocks, and rate limits. Use when the user wants to turn on WAF, block traffic by IP or country, rate-limit login or signup pages, protect pages from brute-force attempts, restrict access to specific paths, review the current firewall configuration, or asks "is my site protected against bots / common web attacks?" — even if they say "add rate limit" or "protect login page" without mentioning "firewall" or "WAF".

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Use when lighting or rendering in Unreal Engine 5.8: physical light units and exposure (EV100, lux, lumens), Lumen GI and reflections, MegaLights, virtual shadow maps, sky, fog and clouds, post process and color grading, the path tracer, or Movie Render Graph stills. Triggers: "Lumen is noisy", "MegaLights noise", "the interior is too dark", "light leaks", neon signs, a night or dusk scene, "fireflies", "path tracer vs Lumen", "render a still", EXR settings.

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Use when building gameplay systems in Godot 4.7: enemy AI that patrols, chases and attacks, state machines, behavior trees (LimboAI), NavigationAgent3D pathfinding and avoidance for 100 to 1000 enemies, 'agents stack up' or 'path is empty', hitbox and hurtbox combat, fast bullets going through walls, save and load, inventory, quests, dialogue (Dialogue Manager), input remapping, procedural dungeons, or measuring agent counts and frame time headless.

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Architecting cross-tool conversion flows that match audience and stage. Landing page to lead magnet to nurture sequence to offer to advanced funnels. Honest about silo-funnels (every tool standalone), kitchen-sink-funnels (every audience squeezed through one path), and matched-funnels (architecture matched to audience-and-stage) patterns. Triggers on funnel design, conversion architecture, marketing funnel, growth funnel, lifecycle architecture, nurture sequence design, multi-tool funnel orchestration. Also triggers when the team's growth tools are working individually but not together, when audience segments share one nurture path, or when a funnel is being architected from scratch.

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Analyze event logs, clickstreams, user paths, product funnels, retention, behavioral segments, transition graphs, step matrices, sequence patterns, and customer journeys using Retentioneering. Use when the user provides CSV, Parquet, pandas, or database event data containing user, event, and timestamp columns, or asks why users convert, churn, loop, abandon a flow, or follow particular product paths. Do not use for qualitative journey-mapping workshops or aggregate website traffic without user-level event sequences.

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Computational pathology and whole slide image analysis

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Build dependency network and critical path from a matter plan. Produce an interactive Gantt, flag near-critical tasks, and run what-if cascade scenarios when delays occur — showing programme impact and drafting communications. Produces filtered workstream or jurisdiction views for local counsel. Trigger on: 'build a timeline', 'Gantt chart', 'critical path', 'what if X is delayed', 'what moves if', 'schedule impact', 'how does this affect the programme', 'when do we finish', 'can we still close on time', 'if we miss this deadline', 'run a what-if', 'visualise the plan', 'Germany timeline', 'what does the Employment workstream look like', 'timeline for local counsel', 'just show me the [workstream] tasks'.

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When a solution seems impossible and the path forward is blocked. Refuses to take no for an answer: classifies what kind of "no" you hit, then runs lateral-thinking techniques (assumption autopsy, inversion, first principles, constraint toggling, analogical transfer, and more) until the wall cracks or is proven load-bearing. Generates at least 10 angles before evaluating any. AGENTS: use this on yourself when an API doesn't support what you need, an approach has failed twice, or a "that's not possible" sentence is forming — run the fast path BEFORE reporting a dead end. Archives walls to ~/.config/makerskills/unstuck/archive/. Sits upstream of decide. Triggers on "/unstuck," "I'm stuck," "this seems impossible," "we hit a wall," "there's no way to," "they said no," "dead end," "out of options," "I've tried everything," "work around this."

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Measure and shrink the always-loaded context of a Claude Code harness (CLAUDE.md + rules without paths frontmatter) back under budget — migrate narrative to reference files, convert rules to path-scoped or skill-triggered loading, and verify zero governance loss with deterministic preservation checks. Use on "harness diet", "context diet", "rules diet", "CLAUDE.md too long", "always-load budget", "trim my rules", "context bloat", "하네스 다이어트", "룰 다이어트", "컨텍스트 다이어트", "always-load 줄여줘". Not for one-off prose editing or project docs cleanup.

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Present project-design pathway options and orchestrate regenerative projects using backwards design, PBL, SEEDS, compassionate systems action, or civic/service pathways.

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Design structured perspective-taking activities with anti-projection guardrails. Develops genuine understanding of complexity across history, social sciences, and literature — not performed empathy.

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Present pathway options and orchestrate place-based curriculum design from a local place, curriculum requirement, or community issue. Use when place should become a primary text for learning.

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Expert blueprint for tower defense games (Bloons TD, Kingdom Rush, Fieldrunners) covering wave management, tower targeting logic, path algorithms, economy balance, and mazing mechanics. Use when building TD, lane defense, or tower placement strategy games. Keywords tower defense, wave spawner, pathfinding, targeting priority, mazing, NavigationServer baking.

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Expert blueprint for real-time strategy games including unit selection (drag box, shift-add), command systems (move, attack, gather), pathfinding (NavigationAgent2D with RVO avoidance), fog of war (SubViewport mask shader), resource economy (gather/build loop), and AI opponents (behavior trees, utility AI). Use for base-building RTS or tactical combat games. Trigger keywords: RTS, unit_selection, command_system, fog_of_war, pathfinding_RVO, resource_economy, command_queue.

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Expert patterns for RPG/action ability systems including cooldown strategies, combo systems, ability chaining, skill trees with prerequisites, upgrade paths, and resource management. Use when implementing unlockable abilities, character progression, or complex skill systems. Trigger keywords: PlayerAbility, AbilityManager, cooldown, SkillTree, SkillNode, prerequisites, can_use, execute, ComboSystem, ability_chain, global_cooldown, charge_system, upgrade_path.

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This package provides the visualization of bayesian network inferred from gene expression data. The networks are based on enrichment analysis results inferred from packages including clusterProfiler and ReactomePA. The networks between pathways and genes inside the pathways can be inferred and visualized.

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Reactome is a free, open-source, curated and peer-reviewed pathway database. Their goal is to provide intuitive bioinformatics tools for the visualization, interpretation and analysis of pathway knowledge.

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Design the optimal funnel from click to conversion. Trigger on "funnel", "conversion path", "booked calls".

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Design the complete free-to-premium value ladder for affiliate promotions. Triggers on: "value ladder", "customer journey", "upsell path", "ascension model", "free to paid funnel", "tripwire offer", "upsell strategy", "downsell", "product ladder", "price ladder", "customer ascension", "funnel architecture", "map my funnel", "design my funnel stages", "monetization path".

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Use when you need to refactor Java code for high performance — including memory/allocation reduction, CPU hot-path optimization, and syntax/API/control-flow improvements. This should trigger for requests such as Review Java code for high performance; Optimize Java hot path; Reduce Java allocations; Improve Java latency/throughput. Part of Plinth Toolkit

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Query the ClinPGx (formerly PharmGKB) REST API plus the CPIC PostgREST companion API for pharmacogenomic clinical annotations, CPIC/DPWG dosing guidelines, gene-drug pairs, variant-drug associations, FDA/EMA drug labels, and PGx pathways. Two-host architecture: api.clinpgx.org for annotation records, api.cpicpgx.org for genotype→recommendation lookups. No auth. For germline pathogenicity use clinvar-database; for somatic cancer PGx use cosmic-database or opentargets-database; for drug bioactivity use chembl-database-bioactivity.

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Query Reactome pathways via REST: pathway queries, entity lookup, keyword search, gene list enrichment, hierarchy, cross-refs. Content + Analysis services. Python wrapper: reactome2py. For KEGG use kegg-database; for PPIs use string-database-ppi.

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