「ssl」の検索結果

End-to-end CLIP-seq pipeline from FASTQ to ENCODE-compliant binding sites, single-nucleotide crosslink maps, annotation, motifs, and (optionally) differential binding. Use when running the full Yeo lab eCLIP / iCLIP / iCLIP2 / iCLIP3 / irCLIP / PAR-CLIP analysis with SMInput control, protocol-specific UMI extraction, ENCODE STAR parameters, CLIPper or Skipper peak calling with stringent log2 FC and -log10 p thresholds, IDR rescue and self-consistency QC, and downstream motif registration with mCross or PEKA.

日本語の概要は準備中です。原文の説明を表示しています。

Preprocess CLIP-seq reads (eCLIP, iCLIP, iCLIP2, iCLIP3, irCLIP, PAR-CLIP, FLASH) with protocol-specific UMI extraction, adapter trimming, length filtering, and post-alignment PCR-duplicate collapse. Use when raw CLIP FASTQ must be turned into deduplicated, crosslink-preserving BAM input for peak calling; choosing between two-pass and single-pass adapter trimming; deciding minimum read length; or mapping UMI patterns to specific eCLIP/iCLIP/iCLIP2/iCLIP3 library preps.

日本語の概要は準備中です。原文の説明を表示しています。

Call protein-RNA binding sites from CLIP-seq BAM with CLIPper, PureCLIP, Skipper, Piranha, omniCLIP, CTK, CLAM, or Paraclu. Use when choosing between coverage-based, HMM-based, beta-binomial window-based, and crosslink-site-based peak callers; applying ENCODE eCLIP thresholds (log2 IP/SMInput >= 3, -log10 p >= 3); deciding when SMInput is mandatory; or reconciling peak-set discordance between callers for the same RBP.

日本語の概要は準備中です。原文の説明を表示しています。

Annotate CLIP-seq peaks or crosslink sites to RNA features (5'UTR, CDS, 3'UTR, intron, splice junction, snoRNA, tRNA, ncRNA, repeat elements) with ChIPseeker, RCAS, RBP-Maps (Yeo splicing regulatory maps), and bedtools, applying feature-priority hierarchies, transcript-context resolution, and metagene aggregation. Use when characterizing where in transcripts an RBP binds, comparing peak distribution across regions, generating splicing-regulatory maps relative to alternative-splicing events, or distinguishing exonic vs intronic vs UTR binding.

日本語の概要は準備中です。原文の説明を表示しています。

Dokku is a self-hosted Platform-as-a-Service built on Docker that provides Heroku-like git-push deployment on your own infrastructure. With 32,000+ GitHub stars, it supports buildpacks, Dockerfiles, custom domains, SSL via Let's Encrypt, and a rich plugin ecosystem for databases, caching, and storage.

日本語の概要は準備中です。原文の説明を表示しています。

Turn a text prompt, a photo, or a live video clip into hand-made-looking p5.js animation — pencil handwriting that writes itself, photos repainted as brushstroke art with paint-on animation and camera moves, and short videos (iPhone Live Photos included) repainted frame-by-frame as a living painting. Trigger on: "handwritten text animation", "paint this photo", "painted video", "sketch effect", "turn my live photo into a painting", "write-on effect". Everything is 100% code: p5.brush strokes rendered headlessly, no image models.

日本語の概要は準備中です。原文の説明を表示しています。

Grill the user relentlessly about a plan, decision, or idea. Use when the user wants to stress-test their thinking, or uses any 'grill' trigger phrases.

日本語の概要は準備中です。原文の説明を表示しています。

Censys search engine for internet-connected hosts, TLS certificates, and web properties. Use when: certificate transparency monitoring, finding hosts by certificate fingerprint, alternative to Shodan for TLS/SSL analysis, discovering hosts running specific services, or tracking infrastructure changes via cert issuance.

日本語の概要は準備中です。原文の説明を表示しています。

What makes founders succeed? Paul Graham has observed thousands of founders and distilled patterns. Relentlessly resourceful. Fierce nerds. Earnest. These traits matter more than credentials or connections. This skill synthesizes PG's essays on founder character into actionable guidance for developing the traits that predict success. Use when "keywords, contexts, " mentioned.

日本語の概要は準備中です。原文の説明を表示しています。

Expert in designing and implementing visceral, satisfying combat systems. Masters hitbox/hurtbox design, frame data, combo systems, enemy archetypes, damage feedback, and the invisible craft that makes players feel powerful. Draws from fighting games, character action games (DMC, Bayonetta), and Souls-like design to create combat that is readable, responsive, and endlessly replayable. Use when "combat system, combat design, hitbox, hurtbox, frame data, hitstop, screen shake, input buffer, coyote time, i-frames, invincibility frames, combo system, attack cancel, recovery frames, punishment window, souls-like combat, action game combat, fighting game, damage feedback, enemy design, boss design, attack telegraph, parry system, stamina system, poise system, weapon feel, game feel combat, melee combat, combat juice, combat, action-game, fighting-game, hitbox, frame-data, game-feel, souls-like, character-action, melee, enemy-design, boss-design, combo, parry, i-frames" mentioned.

日本語の概要は準備中です。原文の説明を表示しています。

Grill the user relentlessly about a plan, decision, or idea. Use when the user wants to stress-test their thinking, or uses any 'grill' trigger phrases.

日本語の概要は準備中です。原文の説明を表示しています。

This skill should be used when the user asks to "make a video with Remotion", "create a programmatic/data-driven video in React", "render an MP4/GIF from code", "animate with useCurrentFrame/interpolate/spring", "sync video to audio/beats", or "render a templated video per record headlessly". Covers building React compositions and rendering them via CLI or @remotion/renderer.

日本語の概要は準備中です。原文の説明を表示しています。

Help user buy a domain, configure DNS, set up SSL. Guide every click.

日本語の概要は準備中です。原文の説明を表示しています。

Final go-live checklist. Is it accessible? SSL working? Mobile friendly? Show user their live URL.

日本語の概要は準備中です。原文の説明を表示しています。

Interview the user relentlessly about a plan or design until reaching shared understanding, resolving each branch of the decision tree. Use when user wants to stress-test a plan, get grilled on their design, or mentions "grill me".

日本語の概要は準備中です。原文の説明を表示しています。

Craft Cloud — Pixel & Tonic's serverless hosting platform for Craft CMS. Covers craft-cloud.yaml configuration, the Build → Migrate → Release deploy pipeline, the craftcms/cloud extension package, edge image transforms via Cloudflare, edge static caching with cache.rules + ESI, Cloud-managed S3 filesystem, MySQL 8 / Postgres 15 databases (no MariaDB, no tablePrefix), Console-based command runner and scheduled cron (once-per-hour minimum), auto-handled queue jobs, custom domains and SSL, preview environments per branch, Cloud limitations (ephemeral filesystem, no SSH, no .htaccess, no built-in mail), plugin development requirements for Cloud compatibility, and self-hosted → Cloud migration.

日本語の概要は準備中です。原文の説明を表示しています。

Grill the user relentlessly about a plan, decision, or idea. Use when the user wants to stress-test their thinking, or uses any 'grill' trigger phrases.

日本語の概要は準備中です。原文の説明を表示しています。

OpenStack backup operations skill for protecting cloud infrastructure through systematic backup strategies and disaster recovery procedures. Covers database backups (MariaDB full and incremental with mariabackup), configuration backups (globals.yml, inventory, Fernet keys), volume snapshots (Cinder LVM snapshots), image exports (Glance), instance snapshots (Nova), backup encryption (GPG/OpenSSL), retention policies (daily/weekly/monthly rotation), restore procedures (database point-in-time recovery, service rebuild), RPO/RTO planning, and disaster recovery drills. Use when planning backup strategy, scheduling automated backups, testing restore procedures, or executing disaster recovery.

日本語の概要は準備中です。原文の説明を表示しています。

Deploy containerized applications to AWS EC2 and expose them publicly via Cloudflare Tunnel with automatic HTTPS. Eliminates need for load balancers, SSL certificates, or public inbound ports.

日本語の概要は準備中です。原文の説明を表示しています。

Synthesize multiple independent structured review verdicts into one lossless deduplicated punch list. Use when findings from several review perspectives must be merged without inventing, dropping, or changing source verdicts.

日本語の概要は準備中です。原文の説明を表示しています。

Manage Alibaba Cloud Redis (Tair / R-KVStore) using the @alicloud/r-kvstore20150101 TypeScript SDK. Use when working with Redis or Tair instances, accounts, backups, security (whitelist/SSL/TDE/audit), parameters, monitoring, cluster scaling, direct connection, Tair Custom instances, and resource tagging. Covers all 157 APIs of the R-KVStore 20150101 version.

日本語の概要は準備中です。原文の説明を表示しています。

Manage Alibaba Cloud DNS (Alidns) using the @alicloud/alidns20150109 TypeScript SDK. Use when working with DNS resolution on Alibaba Cloud, including domain management, DNS record CRUD (A, AAAA, CNAME, MX, TXT, SRV, CAA, etc.), DNS load balancing (DNSSLB), custom resolution lines, DNSSEC, domain groups, batch operations, Cloud GTM (Global Traffic Manager), DNS GTM, GTM Classic, recursive DNS, DNS cache, Public DNS (PDNS), DNS over HTTPS (DoH), ISP cache flush, domain statistics, and resource tagging. Covers all 234 APIs of the Alidns 20150109 version.

日本語の概要は準備中です。原文の説明を表示しています。

Manage Alibaba Cloud CDN using the @alicloud/cdn20180510 TypeScript SDK. Use when working with CDN domain acceleration, domain configuration, SSL certificates, cache refresh/prefetch, real-time monitoring, traffic analysis, log management, usage/billing, IP tools, Function Compute triggers, delivery tasks, and resource tagging. Covers all 168 APIs of the CDN 20180510 version.

日本語の概要は準備中です。原文の説明を表示しています。

Challenge a thesis by playing "Devil's Advocate" (Latin: "Advocatus Diaboli"). Use when the user wants a thesis or statement relentlessly challenged or criticised.

日本語の概要は準備中です。原文の説明を表示しています。