「ept」の検索結果

Validates an approved plan across 8 dimensions before build — incomplete acceptance criteria, missing dependencies, unresolved risks. Use after Gate 1, before Test.

日本語の概要は準備中です。原文の説明を表示しています。

Use when negotiating a file-based sprint contract between the generator (developer) and evaluator before code is written. Translates a product-level spec into testable acceptance criteria with rubric tie-ins. Triggers on /mk:sprint-contract, "draft a sprint contract", "negotiate scope for sprint", or before any harness-driven sprint kicks off.

日本語の概要は準備中です。原文の説明を表示しています。

Read-only spec analysis on a Confluence page (+children): requirements, acceptance criteria, gaps, user stories report. NOT page CRUD (mk:confluence-page) or ticket scoring (mk:planning-engine).

日本語の概要は準備中です。原文の説明を表示しています。

Triage reviewer comments on a GitHub PR with receiving-code-review discipline — verify each against the codebase, then accept/push-back/clarify, and optionally reply in-thread. Use when responding to received PR feedback. NOT for giving a review (see mk:review-pr); NOT for implementing the fixes (hand off to mk:fix).

日本語の概要は準備中です。原文の説明を表示しています。

Fast parallel codebase scouting. Spawns multiple Explore subagents to search directories simultaneously, returning a consolidated file map with architecture fingerprint, complexity estimates, and routing suggestions. Use before planning, debugging, or any task spanning multiple directories. NOT for reading a single known file in depth (use Read directly); NOT for semantic find-usages or go-to-definition.

日本語の概要は準備中です。原文の説明を表示しています。

Use when refining a draft user prompt before sending it to a coding agent. Decomposes goal/context/constraints/acceptance/output-format, detects ambiguity and model-coupled framing, then emits a model-agnostic rewrite. Supports --analyze, --score, and --deep. NOT for prompts from scratch (mk:brainstorming), plans/reviews (mk:elicit), implementation plans (mk:plan-creator), or general codebase scouting (mk:scout).

日本語の概要は準備中です。原文の説明を表示しています。

YC Office Hours — product validation for a new product, feature, or side-project concept. Startup mode tests demand; Builder mode develops an unvalidated product idea. Use when the user asks whether something is worth building or has an idea whose problem/value is not yet validated. Proactively suggest when the user describes a new product idea or is exploring whether something is worth building — before any code is written. Use BEFORE a plan exists. For reviewing an existing plan, use /mk:plan-ceo-review.

日本語の概要は準備中です。原文の説明を表示しています。

Deep spec analysis on a Confluence page. Reads page (+ children) via the confluence-as wrapper, produces a structured Spec Research Report with requirements, acceptance criteria, gaps, ambiguities, and suggested user stories for human review. Read-only — does NOT create Jira tickets. Triggers: 'analyze spec for PAGE-ID', 'extract requirements from confluence page', 'spec research on URL'. NOT for raw page CRUD (mk:confluence-page); NOT for ticket complexity scoring (mk:planning-engine).

日本語の概要は準備中です。原文の説明を表示しています。

Experimental behavioral evaluator. Drives a running artifact via browser/curl/CLI and records rubric evidence; the runner is not yet a fully implemented automated evaluation system.

日本語の概要は準備中です。原文の説明を表示しています。

Develop, challenge, and document Chisel processor microarchitecture designs before implementation. Use when discussing or writing design documents for processor pipelines, queues, issue logic, rename, ROB, LSU, caches, MSHRs, forwarding, wakeup, replay, flush, privilege, exceptions, or other cycle-accurate hardware mechanisms; when converting design conversations into stable Markdown specifications; or when reviewing a proposed Chisel CPU design for correctness, timing, area, verification cost, and cross-document consistency.

日本語の概要は準備中です。原文の説明を表示しています。

Rapidly generate up to 25 distinct ad creative hypotheses for testing. Produces a matrix of angles (pain, benefit, social proof, urgency, curiosity, comparison) x formats (static, carousel hook, UGC-style, bold-text overlay) with headline, visual prompt, and rationale for each, then generates the visuals with any image tool. Use when the user wants "ad variations", "ad concepts", "creative testing batch", "give me 25 ads", or paid-social creative ideation. Part of the Creative Production set.

日本語の概要は準備中です。原文の説明を表示しています。

Apply or adapt a dual-theme liquid glass UI board for premium dashboards, workspace launchers, settings surfaces, design-system previews, and glossy interface concepts. Use when Codex should build or restyle interface work with frosted translucent panels, aurora glow accents, pill controls, circular glass actions, blurred color blooms, and a polished light-plus-dark material system.

日本語の概要は準備中です。原文の説明を表示しています。

Research complex questions and synthesize relevant evidence into a clear, cited answer. Useful for competitive and market analysis, company research, prospect discovery, enrichment, historical comparisons, and evaluating conflicting claims. Adapt the depth and format to the question; use when a focused search or single-page read is insufficient.

日本語の概要は準備中です。原文の説明を表示しています。

Identifies differential m6A methylation between conditions from MeRIP-seq paired IP/input data using exomePeak2 (GC-bias-aware differential via its bam_ip/bam_input control + bam_treated_ip/bam_treated_input treatment arms), QNB beta-binomial, MeTDiff HMM, and RADAR, plus the paired-symmetric edgeR/DESeq2-on-peak-counts route when batch/lot covariates need fixed-effect handling that exomePeak2's API does not accept. Covers paired vs unpaired vs interaction designs, batch confounding and per-lot meta-analysis, the stoichiometry-vs-expression-vs-IP-efficiency confound, and effect-size filtering against under-powered N=2 designs. Use when comparing m6A across two or more conditions, choosing between exomePeak2/QNB/RADAR/MeTDiff for a design, handling batch confounding when exomePeak2's API is too rigid, distinguishing real hyper/hypo-methylation from expression shifts, applying effect-size thresholds, or planning orthogonal stoichiometry validation (GLORI/SAC-seq/m6Anet mod_ratio).

日本語の概要は準備中です。原文の説明を表示しています。

Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.

日本語の概要は準備中です。原文の説明を表示しています。

Call germline SNPs and indels with GATK HaplotypeCaller and the GVCF joint-genotyping workflow. Covers the local-reassembly + PairHMM mechanism (why HC beats pileup callers on indels), the -ERC GVCF reference-confidence model and <NON_REF> allele, BQSR-vs-DRAGSTR and --dragen-mode error modeling, allele-specific (AS_) annotations, and edge cases (ploidy, Mutect2 mitochondria mode, sex chromosomes/PAR, contamination gating). Use when deciding whether to use HaplotypeCaller vs a pileup or DRAGEN caller, whether BQSR still earns its place, whether to call per-sample GVCFs for a cohort, or how to handle non-diploid, mitochondrial, sex-chromosome, or contaminated samples. Not for post-calling filtering depth (see variant-calling/filtering-best-practices) or cohort joint-genotyping scaling (see variant-calling/joint-calling).

日本語の概要は準備中です。原文の説明を表示しています。

Designs genomics experiments so technical nuisance variation (batch, lane, plate, flow cell, operator, reagent lot, processing day) is balanced against the biological variable of interest and therefore estimable rather than confounded, using constrained sample-to-batch assignment (designit, OSAT), the confounder/mediator/collider distinction, and the principle that no post-hoc correction recovers a fully confounded design. Covers detecting hidden batches with surrogate variable analysis, a decision table for downstream correction (ComBat-seq, RUVSeq, SVA) whose execution is deferred to differential-expression/batch-correction, and reproducibility metadata. Use when assigning samples to sequencing batches/lanes/plates, avoiding batch-condition confounding, deciding whether a design is salvageable by correction, choosing a correction method, or estimating the number of hidden batches. For the experimental unit, randomization, and blocking concepts see experimental-design/randomization-blocking.

日本語の概要は準備中です。原文の説明を表示しています。

Detects acquired antimicrobial-resistance determinants and chromosomal point-mutation resistance in bacterial assemblies using AMRFinderPlus, ResFinder 4.0 (acquired + PointFinder), CARD-RGI, abritAMR, staramr, and species-specific callers (TB-Profiler, Mykrobe). Harmonises cross-tool output via hAMRonization, contextualises determinants with mobile-genetic-element annotation (MOB-suite, PlasmidFinder, MobileElementFinder, ICEberg), predicts phenotype against EUCAST or CLSI breakpoints, and translates calls into WHO GLASS reporting categories. Use when screening clinical or surveillance isolates for AMR, distinguishing acquired vs intrinsic vs point-mutation resistance, calling rpoB / katG / pncA / gyrA / mgrB mutations, reconciling AMRFinderPlus vs RGI vs ResFinder disagreement, contextualising carbapenemases or mcr alleles on plasmids, predicting susceptibility from genotype against the WHO Mtb 2nd-edition catalogue, or building a hAMRonized multi-lab AMR surveillance pipeline.

日本語の概要は準備中です。原文の説明を表示しています。

Retrieve records from NCBI databases using Biopython Bio.Entrez (EFetch, ESummary). Use when downloading sequences, fetching GenBank/GenPept records, getting document summaries, parsing nested XML, navigating GI deprecation, choosing between rettype+retmode combinations, and parsing into Biopython SeqRecord/SwissProt objects. Covers nucleotide, protein, gene, pubmed, sra, gds, taxonomy, snp, clinvar.

日本語の概要は準備中です。原文の説明を表示しています。

Select colormaps and qualitative palettes for scientific figures using perceptual-uniformity, color-vision-deficiency safety, and luminance-monotonicity criteria. Covers Crameri scientific colormaps, viridis/cividis/magma, Okabe-Ito categorical, ColorBrewer, and the rainbow/jet critique. Use when choosing palettes for heatmaps, scatter, networks, or any encoding where color carries quantitative or categorical meaning.

日本語の概要は準備中です。原文の説明を表示しています。

Performs differential expression on bulk RNA-seq count data with edgeR's negative-binomial GLM and quasi-likelihood F-test framework. Covers DGEList construction, filterByExpr, TMM/TMMwsp normalization, robust dispersion estimation, glmQLFit/glmQLFTest, TREAT for magnitude-bounded hypotheses, contrasts via no-intercept designs, voom and voomWithQualityWeights for heterogeneous samples, and the edgeR v4 bias-corrected APL changes. Use when running bulk DE with edgeR, choosing edgeR over DESeq2 (small n, transcript DE via catchSalmon, large samples), needing TREAT for a fold-change-threshold hypothesis, troubleshooting v3-to-v4 reproducibility, building paired or interaction designs, or handling library-quality heterogeneity.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes single-cell pooled CRISPR screens (Perturb-seq, CROP-seq, Perturb-CITE-seq, ECCITE-seq, multiome) where each cell carries an sgRNA and a scRNA-seq / surface-protein / chromatin readout. Covers experimental design (direct-capture Perturb-seq Dixit 2016 vs CROP-seq 3'UTR-barcoded Datlinger 2017 vs ECCITE-seq vs Multiome), MOI for sgRNA assignment, escaper-cell filtering (Mixscape, Papalexi 2021), SCEPTRE NB GLM + permutation for low-MOI (Barry 2024 Genome Biol 25:124), the Pertpy framework, factor decomposition, genome-scale Perturb-seq (Replogle 2022 Cell, 2.5M cells), and per-perturbation single-cell DE. Use when running a single-cell CRISPR screen, choosing direct-capture vs CROP-seq architecture, filtering escaper cells, performing single-cell DE, integrating Perturb-seq with pathway analysis, scaling to GW CRISPRi via Replogle protocol, or analyzing multi-omics screens.

日本語の概要は準備中です。原文の説明を表示しています。

Visualize copy number profiles, segments, allele-specific tracks, and cohort patterns from CNVkit, GATK, ASCAT, FACETS, Sequenza, and other callers. Covers genome-wide and per-chromosome log2 scatter plots, B-allele-frequency/minor-allele-fraction tracks, ideograms, cohort heatmaps, circos views, and caller-native plots. Use when creating publication CNV figures, choosing which plot answers a given question, diagnosing a wrong diploid baseline visually, displaying loss of heterozygosity, or deciding what depth-only plots cannot reveal.

日本語の概要は準備中です。原文の説明を表示しています。

Generate consensus FASTA sequences by applying VCF variants onto a reference with bcftools consensus, or build viral/amplicon consensus with iVar. Use when reconstructing a sample-specific reference or haplotype, deciding -H haplotype vs IUPAC vs all-ALT projection, masking no-coverage sites so a consensus does not manufacture false reference calls, or setting iVar min-depth/min-frequency policy for surveillance genomes.

日本語の概要は準備中です。原文の説明を表示しています。