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Assigns GO terms, Pfam/InterPro domains, KEGG orthologs, EC numbers, and product names to predicted proteins using eggNOG-mapper (orthology), InterProScan (domain signatures), and KofamScan (KEGG), routing specialized functions to dbCAN/antiSMASH/AMRFinderPlus/SignalP. Covers the orthology-vs-domain-vs-homology paradigms, the annotation-error percolation cascade, domain-presence-is-not-function, GO IEA circularity in enrichment, evidence tiering, and bit-score/coverage thresholds. Use when adding functional annotation to predicted genes, choosing between eggNOG-mapper and InterProScan, or judging how much to trust a functional label.

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Evaluates genome assembly quality across the three orthogonal axes - contiguity (QUAST auN/NG50/NGx, not bare N50), completeness (BUSCO/compleasm gene-space plus Merqury k-mer completeness), and correctness (reference-free Merqury QV, Inspector/CRAQ structural errors, asmgene false-duplication/collapse). Covers why N50 is the most-gamed metric, why QV measured on the polishing reads is circular, distinguishing uncollapsed haplotigs from real WGD, and the EBP/VGP 6.C.Q40 standard. Use when judging whether an assembly is good enough to annotate or publish, comparing assemblers, diagnosing a fragmented or duplicated assembly, or assessing a phased diploid assembly.

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Structures biological experiments so inference is valid by construction, covering Fisher's principles (randomization, replication, local control), the experimental-vs-observational unit distinction and pseudoreplication (Hurlbert 1984; Lazic 2018), randomization mechanics (complete, restricted, stratified, rerandomization, run-order), blocking layouts (randomized complete block, Latin square, incomplete block), factorial designs and interactions, and the split-plot/nested error strata hidden inside multi-batch genomics. Use when deciding the experimental unit and what counts as a replicate, planning randomization and run order, choosing a blocked/factorial/split-plot/nested layout, avoiding pseudoreplication in cell-culture or animal studies, or specifying the random-effects structure of the analysis model. For assigning samples to sequencing batches/lanes/plates and batch-effect correction see experimental-design/batch-design; for regulated clinical-trial randomization see clinical-biostatistics.

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Simulate transcription factor perturbation effects on cell state in silico with CellOracle and Dynamo, and predict transcriptional responses to genetic perturbations with GEARS, scGen, and CPA. Covers the direction-not-magnitude principle, local-linear validity, the GRN/velocity error it inherits, baseline discipline (mean and additive baselines), and the validation gap. Use when predicting TF knockout or overexpression effects, ranking driver TFs for fate transitions, or planning perturbation experiments. For GRN construction see multiomics-grn; for experimental Perturb-seq see single-cell/perturb-seq.

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Detect positive (diversifying / episodic / pervasive) selection using codon dN/dS frameworks. Implements PAML codeml site models (M0/M1a/M2a/M7/M8/M8a), branch models, branch-site model A (Zhang 2005), and HyPhy methods (BUSTED, BUSTED-S, BUSTED-MH, BUSTED-PH, MEME, FEL, FUBAR, aBSREL, SLAC, RELAX, GARD, FUBAR-MH). Includes McDonald-Kreitman framework (asymptotic alpha, impMKT, polyDFE, DFE-alpha, GRAPES) for within-species + divergence inference, RERconverge for trait-correlated rate shifts, CSUBST for convergent substitution, and PhyloAcc for accelerated noncoding evolution. Use when testing adaptive evolution at codons, branches, or full gene; running GARD recombination pre-screen; controlling alignment-error and gBGC false positives; reconciling PAML vs HyPhy results; or performing genome-scale selection scans.

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Provides REST API design standards and best practices for Spring Boot projects. Use when creating or reviewing REST endpoints, DTOs, error handling, pagination, security headers, HATEOAS and architecture patterns.

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FastAPI conventions for REST APIs: routers, Pydantic v2 schemas, dependency injection, error handling, pagination, and project structure. Trigger: When writing any router, endpoint, schema, or dependency in a FastAPI project.

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Use when generating REST controllers, response wrappers, DTOs, error handlers, or any HTTP-facing code. Defines response envelope, HTTP status mapping, pagination, and versioning.

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Create Convex queries, mutations, and actions with proper validation, authentication, and error handling. Use when implementing new API endpoints.

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Run convex-doctor static analysis, interpret findings, and fix issues across security, performance, correctness, schema, and architecture categories. Use when running convex-doctor, fixing convex-doctor warnings or errors, improving the convex-doctor score, or when asked about Convex code quality, static analysis, or linting Convex functions.

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Genera tickets profesionales de soporte para Meta Business Suite / WhatsApp Business API optimizados para recibir prioridad URGENTE 24hr resolution. Úsala SIEMPRE que el usuario mencione crear un ticket a Meta, escalar un caso WABA, apelar un ban o restricción, reportar un false positive, abrir caso de soporte WhatsApp Business, problema de onboarding, rechazo de template, errores de Cloud API, OTP delivery, verification stuck, BM restricted, display name rejection, o cualquier issue de una WhatsApp Business Account. También activa cuando el usuario dice frases como "ayúdame a redactar un caso para Meta", "necesito reportar esto a soporte", "hay que escalar esto a WhatsApp", "ticket para Meta", o describe un problema de una cuenta de cliente de Vambe sin pedir ticket explícitamente pero claramente necesita uno. También activa ante preguntas del tipo "¿cómo apelar esto?" o "¿qué hago si Meta dice X?" relacionadas con WABA, BM o verificación.

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Find root cause of errors, crashes, regressions, failing tests before any fix. Not for code review or new features.

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Revisa cambios de performance de queries ClickHouse y Postgres (vambe-datascience, CRM, réplicas) contra los errores y aprendizajes de la ronda

日本語の概要は準備中です。原文の説明を表示しています。

Bash/Linux terminal patterns. Critical commands, piping, error handling, scripting. Use when working on macOS or Linux systems.

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Write copy following Sentry brand guidelines. Use when writing UI text, error messages, empty states, onboarding flows, 404 pages, documentation, marketing copy, or any user-facing content. Covers both Plain Speech (default) and Sentry Voice tones.

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Bash scripting workflow for creating production-ready shell scripts with defensive patterns, error handling, and testing.

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Modern Angular UI patterns for loading states, error handling, and data display. Use when building UI components, handling async data, or managing component states.

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Builds production-ready REST API endpoints with validation, error handling, authentication, and documentation. Follows best practices for security and scalability.

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Design spec with 98 rules for building CLI tools that AI agents can safely use. Covers structured JSON output, error handling, input contracts, safety guardrails, exit codes, and agent self-description.

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Tools are how AI agents interact with the world. A well-designed tool is the difference between an agent that works and one that hallucinates, fails silently, or costs 10x more tokens than necessary. This skill covers tool design from schema to error handling.

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Convert AmeriFlux ERA5 half-hourly meteorological CSV files into EcoSIM hourly climate forcing NetCDF files, compare generated forcing against overlapping measured tower meteorology when a FULLSET file is available, and report large biases or errors. Use when preparing or validating EcoSIM climate inputs for AmeriFlux sites, checking ERA5 against in situ records, diagnosing forcing discrepancies, or writing climate-quality reports. For non-AmeriFlux paper sites that need a fresh Copernicus CDS ERA5 point download by longitude/latitude, use era5-cds-point-download first.

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Access the Firebase Emulator Suite (Firestore, Auth, Functions, Hub) via REST and Admin SDK without trial-and-error. Use this skill whenever you read/write/query/clear Firestore Emulator data, create test users or obtain ID tokens from the Auth Emulator, verify Functions triggers, seed/export emulator data, or hit unexpected 403/404 from an emulator endpoint. Consult it BEFORE curling any emulator port — it encodes the auth header and host-resolution rules that otherwise cause retries.

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Premium pre-launch waitlist page template — dark charcoal canvas with subtle vignette, drifting portrait video-thumbnail cards, fade-up cascades, glass nav, live-updating join counter, single email form with success/error states. Built for creative-tool / AI / niche-SaaS invite-only launches with hushed exclusive tone, not loud growth-marketing.

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Manages Cargo.lock file updates and resolves --locked flag issues in CI/CD. Triggers when user mentions: - "cargo test --locked failed" - "cannot update the lock file" - "Cargo.lock is out of date" - "PR failed with --locked error" - "fix Cargo.lock"

日本語の概要は準備中です。原文の説明を表示しています。