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Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

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Defines cell populations in flow and spectral cytometry through manual gates (rectangle, polygon, quadrant, boolean) and reproducible automated gating (openCyto gating templates, flowDensity data-driven thresholds, flowClust model-based gates), organized as a hierarchical GatingSet (flowWorkspace) and round-tripped with FlowJo via CytoML. Covers the canonical gate order (time -> debris -> singlets -> live -> lineage), FMO-vs-isotype boundary setting, gate-order dependence and recompute semantics, rare-event/MRD gating, and per-population statistics. Use when building a gating strategy, automating a manual FlowJo scheme across samples, choosing manual vs data-driven gates, or extracting population frequencies.

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Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

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Use when integrating LLMs, chat clients, embeddings, RAG pipelines, or AI agents into Spring Boot. Covers Spring AI ChatClient, prompt templates, embeddings, vector stores, and structured output. Use when user mentions Spring AI, LLM, ChatGPT, Claude, RAG, embeddings.

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Use when building MCP (Model Context Protocol) servers in Java/Spring Boot. Covers tool registration, resource exposure, prompt templates, and production deployment using the official MCP Java SDK. Use when user mentions MCP, AI agent integration, or tool calling.

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Build static sites with Nitro CLI - a Python static site generator using nitro-ui for programmatic HTML generation instead of templates

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Use this skill whenever the user wants to create, read, edit, or manipulate Word documents (.docx) or Word templates (.dotx). Triggers include: any mention of Microsoft Word Documents, such as 'Word doc', 'word document', '.docx', '.dotx', 'microsoft doc'. Also use when extracting or reorganizing content from .docx or .dotx files, inserting or replacing images in documents, find-and-replace in Word files, working with tracked changes or comments, or converting content into a polished Word document. If the user asks for a deliverable as a Word or .docx file (to download, email or print), use this skill. However, if they ask for a document, page, report, memo, or notes WITHOUT naming a file format and the session offers Claude's own dedicated document or page skill or connector, use that instead, even if they will email or print it. Do NOT use for PDFs, spreadsheets, Google Docs, or coding unrelated to document generation.

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Genera tickets profesionales de soporte para Meta Business Suite / WhatsApp Business API optimizados para recibir prioridad URGENTE 24hr resolution. Úsala SIEMPRE que el usuario mencione crear un ticket a Meta, escalar un caso WABA, apelar un ban o restricción, reportar un false positive, abrir caso de soporte WhatsApp Business, problema de onboarding, rechazo de template, errores de Cloud API, OTP delivery, verification stuck, BM restricted, display name rejection, o cualquier issue de una WhatsApp Business Account. También activa cuando el usuario dice frases como "ayúdame a redactar un caso para Meta", "necesito reportar esto a soporte", "hay que escalar esto a WhatsApp", "ticket para Meta", o describe un problema de una cuenta de cliente de Vambe sin pedir ticket explícitamente pero claramente necesita uno. También activa ante preguntas del tipo "¿cómo apelar esto?" o "¿qué hago si Meta dice X?" relacionadas con WABA, BM o verificación.

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Use this skill any time a .pptx or .potx file is involved in any way — as input, output, or both. This includes: creating slide decks, pitch decks, or presentations as PowerPoint (.pptx) files; reading, parsing, or extracting text from any .pptx or .potx file (even if the extracted content will be used elsewhere, like in an email, summary, or creating a different type of slide deck); editing, modifying, or updating existing presentations; combining or splitting slide files; working with templates (.potx), layouts, speaker notes, or comments. Trigger whenever the user asks for a PowerPoint or .pptx file, or references a .pptx or .potx filename, regardless of what they plan to do with the content afterward. However, when the user asks for a deck, slides, a slide deck, or a presentation without naming a file format, default to using a dedicated slide-deck artifact type or a separate slides skill if this session offers one; otherwise, use this skill.

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Map observed site vegetation, species lists, land-cover descriptions, or plant management PFTs to EcoSIM vegetation codes using templates/ecosim_pftpar_20260303.nc.cdl. Use when choosing or validating EcoSIM pft_type values, pfts entries, pfts_short categories, pfts_long plant type meanings, or the combined short-code plus Koppen climate numerical code used by plant management inputs.

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Extract dominant-component soil profile variables for EcoSIM grid or template inputs from gSSURGO, with FAO HWSD v2.0 fallback when gSSURGO data are unavailable or incomplete. Use when deriving soil depth, bulk density, field capacity, wilting point, hydraulic conductivity, texture, rock fraction, pH, CEC, or soil organic carbon; or when converting EcoSIM grid NetCDF files to editable Excel workbooks and back.

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投行 strategic-alternatives pitch book — trading comps / precedent transactions / valuation football field / DCF sensitivity / 战略选项矩阵 / 流程建议。改写 assets/template.html 保留 IB-specific chrome / disclosure bands / source labels。用于 Board / sell-side 讨论材料。**不是** VC 融资 deck。工作流改自 Anthropic financial-services Pitch Agent (Apache-2.0)。

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Chinese-first AI image-generation tool landing template — TwelveMei-style centered hero with floating icon-island nav, FlyFlowerSong serif display headline, textured pill CTAs, glass canvas mockup of the tool itself, monochrome ink palette. Built for Chinese creative-tool / generative-image / AI-工具 product pages aimed at designers and editors, not loud growth-marketing.

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Long-form Chinese article / blog-post template — two voices: a wildstay-style lifestyle launch piece with editorial grid + oversized display type, and a WeChat-公众号-style serif beauty/longform with measured prose and quiet hairlines.

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Chinese-first internal-tool / ops dashboard template — light-mode Linear-grade layout with serif headlines, tabular Inter numerals, KPI hero, pulse bars, progress rings, team-presence grid, activity timeline, and CJK-aware typography.

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Notebook-collage weekly digest template — spiral-bound reporter's notebook aesthetic with cobalt tape, mint folder tab, binder clip, receipt block, ruled baseline grid; built for AI-product / team / design weekly digests delivered as a downloadable high-res page.

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Premium calm-tech SaaS landing template — bilingual (CN/EN) literary voice over a dark forest-night palette with warm gold accent, Cormorant + Noto Serif SC, Three.js procedural forest hero, station-based scrolling narrative, custom cursor. Built for ambient-AI / creative-tool / philosophical SaaS, not loud commercial launch.

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Premium "digital e-guide" template family — two patterns sharing a metadata-rich, editorial-archive identity: an infinite 3D-canvas portfolio (case/) for designer 作品集, and a 12-slide horizontal field-notes carousel (social-carousel.html) for long-form knowledge series.

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Hand-drawn paper-wireframe template — white paper background, monochromatic ink palette, SVG turbulence "wobble" filters for pen-quiver authenticity, handwritten fonts (Caveat / Kalam / Ma Shan Zheng / Long Cang), wavy dividers, sketch-rect overlays, marginalia annotations. For PRD illustrations, IA flow docs, low-fidelity layout exploration, "draft / not final" stakeholder communication. Three skeletons (single-page editorial / app multi-screen flow / IA sitemap) — pick one up front based on the artifact, do NOT inherit only the brush strokes.

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Chinese-market SaaS pricing page template — soft sky-blue glass-morphism, 4-tier card grid with one elevated "recommended" tier, monthly/annual toggle, FAQ accordion, ¥ pricing with Inter numerals, careful CJK linebreaking, ICP filing footer.

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小红书 (XHS) 3:4 vertical cover template — two voices: spring/lifestyle soft watercolor cover with Songti + ink-green accents, and bold editorial typography cover with cobalt-blue bands + folded-paper interior. Despite the "ppt" prefix in the path, these are fixed-image XHS covers, not slide decks.

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Premium pre-launch waitlist page template — dark charcoal canvas with subtle vignette, drifting portrait video-thumbnail cards, fade-up cascades, glass nav, live-updating join counter, single email form with success/error states. Built for creative-tool / AI / niche-SaaS invite-only launches with hushed exclusive tone, not loud growth-marketing.

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Single-track / single-episode music-player mockup template — glassmorphic iOS-Music style player card with ambient album-art wash, for showcasing one song, one podcast episode, or one audio jingle as a shareable interactive surface.

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Team operations dashboard template — Linear/Asana-clean hybrid with sprint status hero, 4-segment progress bar, workstream cards, chronological activity stream with colored event badges, right-rail with today's schedule + team-presence + upcoming milestones. For team status snapshots, OKR reviews, "team this week" all-hands views.

日本語の概要は準備中です。原文の説明を表示しています。