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Calculates statistical power for high-dimensional genomics experiments (bulk RNA-seq, scRNA-seq, ATAC-seq, ChIP-seq, methylation, proteomics) under negative-binomial count models using RNASeqPower, PROPER, and simulation via powsimR, distinguishing per-gene from marginal (transcriptome-wide) power, the role of mean expression and dispersion, and the sequencing-depth-versus-replicate tradeoff. Covers simulation as the honest default for overdispersed counts, FDR-aware average power versus single-test power, observed/post-hoc power as an anti-pattern, and the winner's-curse / Type-S / Type-M consequences of underpowering. Use when planning replicate number for a sequencing experiment, deciding whether to add depth or samples, choosing closed-form versus simulation power, estimating power from pilot dispersions, or justifying replication in a grant. For clinical-trial power see clinical-biostatistics/power-and-sample-size; for the inverse sample-size question see experimental-design/sample-size.

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Plot per-group distributions of continuous data using boxplots, violins, beeswarms, quasirandom jitter, and raincloud plots with sample-size honesty (Weissgerber 2015), KDE-bandwidth awareness, and N-aware encoding choices. Use when comparing distributions across a small number of groups — expression per cluster, biomarker per arm, scores per condition — and the bar-of-mean default is misleading.

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Build forest plots (HR, OR, RR, beta-coefficient summaries with CIs) and funnel plots (meta-analysis publication-bias diagnostics) using forestplot, metafor, ggforest, and MendelianRandomization with proper axis-scaling, summary-diamond placement, subgroup nesting, and Egger / trim-and-fill asymmetry tests. Use when summarizing effects across subgroups, trials, or instruments — meta-analysis, Mendelian randomization, subgroup HRs.

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Retrieve records from NCBI databases using Biopython Bio.Entrez (EFetch, ESummary). Use when downloading sequences, fetching GenBank/GenPept records, getting document summaries, parsing nested XML, navigating GI deprecation, choosing between rettype+retmode combinations, and parsing into Biopython SeqRecord/SwissProt objects. Covers nucleotide, protein, gene, pubmed, sra, gds, taxonomy, snp, clinvar.

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Implement Swift Codable models for JSON and property-list encoding and decoding with JSONDecoder, JSONEncoder, CodingKeys, and custom init(from:) or encode(to:). Use when parsing API responses, remapping keys, flattening nested JSON, handling date or data decoding strategies, decoding heterogeneous arrays, or integrating Codable with URLSession, SwiftData, or UserDefaults.

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Apply modern Swift language patterns and idioms for non-concurrency, non-SwiftUI code. Covers if/switch expressions (Swift 5.9+), typed throws (Swift 6+), result builders, property wrappers, opaque and existential types (some vs any), guard patterns, Never type, Regex builders (Swift 5.7+), basic Codable shaping (CodingKeys, custom decoding, nested containers), modern collection APIs (count(where:), contains(where:), replacing()), basic FormatStyle usage, and string interpolation patterns. Use when writing core Swift code involving generics, protocols, enums, closures, or modern language features; route deep Codable to swift-codable, detailed formatting/localization to swift-formatstyle, and API naming to swift-api-design-guidelines.

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Microstrip, stripline, CPW, differential pairs, and crosstalk analysis for impedance-controlled PCB interconnects. TRIGGER: user asks to design or analyze a transmission line (microstrip, stripline, CPW, coplanar, differential pair), extract RLGC or per-unit-length parameters, compute trace impedance, analyze a PCB trace cross-section, or perform crosstalk/coupling analysis. Invoke BEFORE writing code — preferred over RF Toolbox analytical functions (txlineMicrostrip, txlineStripline, txlineCPW). SKIP: EM simulation/S-parameter extraction of an existing component (use matlab-analyze-em), material/stackup definition only (use matlab-manage-pcb-material), building custom non-catalog geometry (use matlab-assemble-pcb-layout), optimization sweeps (use matlab-optimize-pcb-design).

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AWS CloudFormation infrastructure as code for stack management. Use when writing templates, deploying stacks, managing drift, troubleshooting deployments, or organizing infrastructure with nested stacks.

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Deploy AWS resources with CloudFormation templates. Create stacks, use nested stacks, and implement drift detection. Use when deploying AWS-native IaC.

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Discover and load all relevant context for the current task. Lists feature group nested files with full paths, loads process/context/ files by domain routing. Called at the start of every agent session.

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Use this skill for Node.js backend AI via @cloudbase/node-sdk (>=3.16.0) — cloud functions, CloudRun, Express/Koa/NestJS, serverless APIs, scheduled jobs, LLM proxies, agent orchestration. The only SDK supporting image generation (ai.createImageModel + generateImage). Text via ai.createModel with groups cloudbase, hunyuan-exp, or custom-*; model ids (e.g. deepseek-v4-flash, glm-5, kimi-k2.6) go in the `model` field of generateText/streamText. MUST run two-step preflight before code — see body. NOT for browser/Web (use ai-model-web) or Mini Program (use ai-model-wechat).

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Designing in-product tours, tooltips, and contextual help that teach product capabilities without becoming friction. Trigger logic, tour architecture, contextual placement, completion tracking. Honest about tooltip-spam (visual noise that users develop blindness to), one-and-done (help invisible at the moment of need), and contextual-when-needed (surfaces help at the moment friction occurs) patterns. Triggers on product tour, in-product tooltip, contextual help, walkthrough, feature tour, hint system, in-app guidance, tour platform. Also triggers when feature adoption is low, when users miss key product capabilities, or when an in-product help system is being scoped for the first time.

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Designing forms with multiple steps, progress indicators, conditional logic, and save-and-resume mechanics. The discipline of breaking complex data collection into stages that respect cognitive load while maintaining completion intent. Honest about kitchen-sink-single-page (overwhelms before the user starts), progress-theater (steps without genuine staging), and genuinely-staged (each step earns its own page) patterns. Triggers on multi-step form, multi-page form, form wizard, signup wizard, lead form, application form, intake form, configurator, onboarding form. Also triggers when a long form is converting poorly, when an audience is dropping off mid-form, or when a multi-step form is being scoped for the first time.

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The Jobs-to-be-Done framework as applied product methodology. Job statements, struggling moments, hire and fire criteria, the difference between feature-thinking and job-thinking. Honest about where JTBD adds clarity (discovery, prioritization, positioning) and where it becomes performative ritual (job-statement workshops that do not drive decisions, persona-theater disguised as JTBD). Triggers on jobs-to-be-done, JTBD, job statements, struggling moments, hire criteria, fire criteria, switch triggers, functional emotional social jobs, outcome-driven innovation. Also triggers when a team is over-relying on feature-request lists or persona archetypes that do not drive product decisions, when a positioning conversation needs the framing JTBD provides, or when discovery is producing outputs that do not connect to product strategy.

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Designing quizzes, personality assessments, and recommendation tools that segment users into actionable categories rather than generating clicks for clicks' sake. Question architecture, scoring algorithms, result categorization, recommendation mapping, lead capture integration. Honest about clickbait-quiz (engagement only), vanity-result (entertaining, not useful), and actionable-segmentation (genuine categorization that drives next-step recommendations) patterns. Triggers on quiz, assessment, personality test, recommendation tool, scorecard, diagnostic, fit evaluator, what-type-of-X-are-you, persona quiz. Also triggers when an audience needs a categorization-driven lead magnet, when a vanity quiz is producing engagement but no qualified leads, or when an assessment is being scoped for the first time.

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Designing lead magnets that earn the email. The discipline of building gated content (ebooks, templates, checklists, swipe files, mini-courses, free tools) that delivers genuine standalone value while qualifying the lead and warming them for what comes next. Honest about thin-bait (overpromises, underdelivers), kitchen-sink-resource (everything, helps with nothing), and earned-value-magnet (delivers standalone value while qualifying the lead). Triggers on lead magnet, gated content, opt-in offer, ebook, checklist, template, swipe file, mini-course, free tool, content upgrade, freebie, opt-in, list-building offer. Also triggers when an audience is being asked for an email and the offer attached to that ask needs design discipline, when previous lead magnets converted but did not produce qualified leads, or when a lead magnet is being scoped for the first time.

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Designing first-run product onboarding wizards that get users to the ah-ha moment without overwhelming them. Step architecture, progressive disclosure, escape hatches, completion incentives, drop-off measurement. Honest about tutorial-overload (dump everything upfront), skip-friendly-empty (skipped onboarding leads to abandoned product), and earned-progressive-disclosure (right things at the right moments) patterns. Triggers on onboarding wizard, product onboarding, first-run experience, signup flow, activation flow, FRX, time-to-value, ah-ha moment design. Also triggers when activation rates are low, when users skip onboarding and never return, when onboarding flows are being scoped for the first time, or when audience research shows users not finding key features.

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Architecting cross-tool conversion flows that match audience and stage. Landing page to lead magnet to nurture sequence to offer to advanced funnels. Honest about silo-funnels (every tool standalone), kitchen-sink-funnels (every audience squeezed through one path), and matched-funnels (architecture matched to audience-and-stage) patterns. Triggers on funnel design, conversion architecture, marketing funnel, growth funnel, lifecycle architecture, nurture sequence design, multi-tool funnel orchestration. Also triggers when the team's growth tools are working individually but not together, when audience segments share one nurture path, or when a funnel is being architected from scratch.

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Designing free-to-paid conversion flows for SaaS products. Trigger moments, paywall design, value demonstration, upsell vs downsell, win-back flows, churn prevention. Honest about paywall-everywhere (gates everything aggressively), free-forever-trap (no upgrade path surfaces), and value-triggered-upgrade (paywall surfaces at moments of demonstrated value) patterns. Triggers on upgrade flow, paywall, free-to-paid, freemium conversion, trial conversion, plan upgrade, subscription upgrade, win-back flow, churn prevention. Also triggers when free-to-paid conversion is low, when paywalls are blocking the wrong moments, or when upgrade flows are being scoped for the first time.

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Designing build-your-own product configurators (Tesla-style, custom-pricing, plan-builders) with constraint logic, real-time pricing, validation, and save-and-share mechanics. Honest about infinite-options (decision paralysis), canned-bundles-only (no real customization), and guided-configuration (smart defaults plus meaningful constraints plus escape hatches) patterns. Triggers on configurator design, build-your-own, custom configuration, plan builder, product customizer, configuration tool. Also triggers when users abandon mid-configuration, when configurator conversion is poor, or when a configurator is being scoped for the first time.

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Designing meeting schedulers and booking experiences that qualify leads, set up calls well, and convert at higher rates than a generic Calendly link. Availability logic, qualification gating, prep automation, follow-up sequencing. Honest about any-time-friction (no qualification, just a booking link), interrogation-gate (so much qualification it scares users off), and qualified-fast-path (just enough qualification to set up the call well) patterns. Triggers on scheduler design, meeting booking, demo scheduling, sales call scheduling, calendar tool, booking page, qualification flow. Also triggers when sales team complains about cold demos, when booking conversion is poor, or when scheduler is being scoped for the first time.

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How humans and AI compose in content workflows. Where AI legitimately participates, where humans must own, hybrid workflow patterns, voice ownership preservation, the AI slop problem, disclosure and transparency, team calibration, and the ethics of intellectually honest AI-assisted content production. Triggers on AI content workflow, AI-assisted writing, hybrid content production, AI in editorial, AI slop, AI disclosure, AI usage policy, AI content ethics, voice preservation with AI, team AI calibration. Also triggers when content feels generic despite quality tools, when team AI usage has drifted into inconsistency, or when a regulated or trust-sensitive context requires explicit AI policy.

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Designing conversational flows for website chatbots and AI agents. Intent recognition architecture, branching logic, fallback handling, escalation to human, conversation analytics. Honest about scripted-bot (rigid trees, fail edge cases), hallucinating-bot (LLM without structure, makes things up), and structured-guided-conversation (LLM-powered with intent architecture and fallback discipline) patterns. Distinguishes chatbot DESIGN (this skill) from chatbot IMPLEMENTATION (engineering and platform work). Triggers on chatbot, conversational AI, AI agent, chat widget, intent design, conversational flow, bot escalation, LLM grounding. Also triggers when a chatbot is hallucinating, when a scripted bot is failing edge cases, or when a chatbot is being scoped for the first time.

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Designing interactive calculators (ROI calculators, pricing estimators, savings projections, mortgage calculators, custom assessments) that deliver real decision-support value while serving as lead magnets and qualified-traffic generators. Honest about vanity-calculator (no real value), lead-trap (hides the answer behind email), and transparent-decision-tool (gives the result and earns the email through tiered value) patterns. Triggers on calculator, ROI calculator, pricing estimator, savings calculator, custom calculator, interactive tool, decision tool, financial calculator. Also triggers when an audience needs a calculation-driven lead magnet, when a vanity calculator is producing leads but no qualified ones, or when a calculator is being scoped for the first time.

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