4,477 件 ・ 関連度順
概要と使いどころ
End-to-end CRISPR experiment design from target selection to delivery-ready constructs. Covers guide RNA design, off-target assessment, and specialized editing strategies including knockouts, base editing, and HDR knockins. Use when designing complete CRISPR editing experiments for gene knockout, correction, or tagging.
日本語の概要は準備中です。原文の説明を表示しています。
Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Cell Reports 36:109597), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.
日本語の概要は準備中です。原文の説明を表示しています。
Clinical variant interpretation using ClinVar, ACMG guidelines, and pathogenicity predictors. Prioritize variants for diagnostic and research applications. Use when interpreting clinical significance of variants.
日本語の概要は準備中です。原文の説明を表示しています。
Design guides for cytosine and adenine base editing using editing window optimization and BE-Hive outcome prediction. Select optimal positions for C-to-T or A-to-G conversions without double-strand breaks. Use when designing base editor experiments for precise nucleotide changes.
日本語の概要は準備中です。原文の説明を表示しています。
Proteina-Complexa flow-based protein backbone generation with fold-conditioned sampling guidance. Use this skill when: (1) Generating de novo protein backbones with hierarchical fold conditioning, (2) Exploring long-chain backbone generation beyond standard diffusion baselines, (3) Using NVIDIA Proteina-style flow matching workflows for controllable backbone design, (4) Comparing flow-based backbone generation against RFdiffusion or BoltzGen, (5) Prototyping fold-guided backbone campaigns before sequence design. This skill is based on the public NVIDIA Digital Bio Proteina project and uses "Proteina-Complexa" as the BioClaw-facing skill label. For sequence design after backbone generation, use proteinmpnn or solublempnn. For QC thresholds, use protein-design-qc.
日本語の概要は準備中です。原文の説明を表示しています。
Automated and marker-guided single-cell cell type annotation using CellTypist, marker review, reference transfer, and confidence-aware label curation.
日本語の概要は準備中です。原文の説明を表示しています。
Structure retrieval, confidence-aware AlphaFold DB usage, coordinate download, PAE and pLDDT interpretation, and structure-guided biological annotation.
日本語の概要は準備中です。原文の説明を表示しています。
End-to-end protein design pipeline guide across preparation, generation, validation, and filtering. Use this skill when: (1) Starting a new protein design project, (2) Need step-by-step workflow guidance, (3) Understanding the full design pipeline, (4) Planning compute resources and timelines, (5) Integrating multiple design tools. For tool selection, use binder-design-tool-selection. For QC thresholds, use protein-design-qc.
日本語の概要は準備中です。原文の説明を表示しています。
codeck entry point. Scans local files for materials, shows pipeline dashboard with diagnostic intelligence, guides user to the next step. Use when the user says "codeck", "new deck", "make a presentation", "make a deck", "new slides", "build a presentation", "export", "speech", "script", or wants to start or continue a presentation project. /codeck is the user-facing entry; legacy sub-skills are internal modules.
日本語の概要は準備中です。原文の説明を表示しています。
Guide human-centered design processes using empathy-driven methodologies. Use when the user says "lets run design thinking" or "I want to apply design thinking"
日本語の概要は準備中です。原文の説明を表示しています。
Guide for creating high-quality MCP (Model Context Protocol) servers that enable LLMs to interact with external services through well-designed tools. Use when building MCP servers to integrate external APIs or services, whether in Python (FastMCP) or Node/TypeScript (MCP SDK).
日本語の概要は準備中です。原文の説明を表示しています。
Comprehensive guidelines for Obsidian.md plugin development including ESLint rules from eslint-plugin-obsidianmd v0.4.2, TypeScript best practices, memory management, API usage (requestUrl vs fetch), UI/UX standards, popout window compatibility, community.obsidian.md submission process, and Scorecard optimization. Use when working with Obsidian plugins, main.ts files, manifest.json, Plugin class, MarkdownView, TFile, vault operations, or any Obsidian API development.
日本語の概要は準備中です。原文の説明を表示しています。
Sequenced launcher that bootstraps QA on a project with no QA in place. Chains qa-project-context → test-strategy → test-planning in one guided run, then points you at automation. Use when: "set up QA on a new project," "QA from scratch," "no QA exists yet," "/qa-start." Not for: onboarding a QA engineer to an existing team with existing tests — use qa-project-bootstrap. Not for: "which skill do I use" with no clear match — use qa-do. Related: qa-project-context, test-strategy, test-planning, qa-project-bootstrap, qa-do.
日本語の概要は準備中です。原文の説明を表示しています。
Use when tutoring a learner through MATLAB debugging, error interpretation, failed tests, incorrect outputs, array-shape problems, indexing mistakes, function argument issues, or code repair practice. Use for guided debugging sessions, debugging drills, teach-the-agent critique, and evidence-based MATLAB troubleshooting.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill for guided MBSE work in MATLAB — starting a new project, resuming work mid-workflow on an existing project, or answering orientation questions about how the MBSE skills fit together. Trigger when the user says they want to create, start, or set up a new MBSE project; work on a model-based systems engineering / RFLPV project; or asks which skill covers which phase. Walks through phases one at a time — propose → approve → generate → run → confirm. Use proactively whenever someone mentions starting or continuing an MBSE project.
日本語の概要は準備中です。原文の説明を表示しています。
Use when prompting a learner to complete hands-on MATLAB coding exercises, guided practice, debugging drills, code tracing, small MATLAB projects, or MATLAB-script assessment during tutoring. Use when the tutor should create a complete runnable MATLAB script, execute it through MATLAB tools, compare the produced outputs with expected outputs, and evaluate MATLAB programming style.
日本語の概要は準備中です。原文の説明を表示しています。
Core agent-browser usage guide. Read this before running any agent-browser commands. Covers the snapshot-and-ref workflow, navigating pages, interacting with elements (click, fill, type, select), extracting text and data, taking screenshots, managing tabs, handling forms and auth, waiting for content, running multiple browser sessions in parallel, and troubleshooting common failures. Use when the user asks to interact with a website, fill a form, click something, extract data, take a screenshot, log into a site, test a web app, or automate any browser task.
日本語の概要は準備中です。原文の説明を表示しています。
Guides God Committee members through executing interventions. Use for repairs, rollbacks, and emergency actions. Triggers on: intervention, repair, rollback, emergency action.
日本語の概要は準備中です。原文の説明を表示しています。
Builds project visions through interactive guided conversation. Use when users have vague ideas needing structure. Triggers on: build vision, I have an idea, start new project, new idea.
日本語の概要は準備中です。原文の説明を表示しています。
Guides God Committee members through consensus-building for collective decisions. Use for proposals, voting, and disagreement resolution. Triggers on: consensus, voting, proposal, committee decision.
日本語の概要は準備中です。原文の説明を表示しています。
Guides parallel exploration of multiple implementation approaches using git worktrees. Use when facing decisions with multiple valid paths. Triggers on: explore options, compare approaches, parallel exploration.
日本語の概要は準備中です。原文の説明を表示しています。
Document development environment requirements for team consistency and AI agent understanding during PRD v0.6 Architecture. Triggers on requests to define environment setup, document tooling, create dev setup guide, or when user asks "what tools do I need?", "environment setup", "dev environment", "CLI requirements", "project setup", "onboarding setup". Consumes TECH- (stack selections), ARC- (architecture decisions). Outputs ENV- entries for development, CI/CD, and infrastructure environments. Feeds v0.7 Build Execution.
日本語の概要は準備中です。原文の説明を表示しています。
Surface risks through guided questioning, helping users consider pivots, constraints, and prioritization during PRD v0.5 Red Team Review. Triggers on requests to identify risks, stress-test the idea, perform red team review, or when user asks "what could go wrong?", "identify risks", "red team", "risk assessment", "challenge assumptions", "stress test the idea". Consumes all prior IDs (CFD-, BR-, FEA-, PER-, UJ-, SCR-) as interview context. Outputs RISK- entries with owner decisions and mitigations. Feeds v0.5 Technical Stack Selection.
日本語の概要は準備中です。原文の説明を表示しています。
Detect template version drift and guide migration to the latest template version. Compares current repo against the template, identifies what's outdated, and automates safe updates while protecting product-specific content. Triggers on requests to sync with template, update template version, check for template drift, or when user asks "sync template", "update to v3", "template drift", "check template version".
日本語の概要は準備中です。原文の説明を表示しています。