Builds production-ready REST API endpoints with validation, error handling, authentication, and documentation. Follows best practices for security and scalability.
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Builds production-ready REST API endpoints with validation, error handling, authentication, and documentation. Follows best practices for security and scalability.
日本語の概要は準備中です。原文の説明を表示しています。
Design spec with 98 rules for building CLI tools that AI agents can safely use. Covers structured JSON output, error handling, input contracts, safety guardrails, exit codes, and agent self-description.
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Assigns GO terms, Pfam/InterPro domains, KEGG orthologs, EC numbers, and product names to predicted proteins using eggNOG-mapper (orthology), InterProScan (domain signatures), and KofamScan (KEGG), routing specialized functions to dbCAN/antiSMASH/AMRFinderPlus/SignalP. Covers the orthology-vs-domain-vs-homology paradigms, the annotation-error percolation cascade, domain-presence-is-not-function, GO IEA circularity in enrichment, evidence tiering, and bit-score/coverage thresholds. Use when adding functional annotation to predicted genes, choosing between eggNOG-mapper and InterProScan, or judging how much to trust a functional label.
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Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.
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Evaluates genome assembly quality across the three orthogonal axes - contiguity (QUAST auN/NG50/NGx, not bare N50), completeness (BUSCO/compleasm gene-space plus Merqury k-mer completeness), and correctness (reference-free Merqury QV, Inspector/CRAQ structural errors, asmgene false-duplication/collapse). Covers why N50 is the most-gamed metric, why QV measured on the polishing reads is circular, distinguishing uncollapsed haplotigs from real WGD, and the EBP/VGP 6.C.Q40 standard. Use when judging whether an assembly is good enough to annotate or publish, comparing assemblers, diagnosing a fragmented or duplicated assembly, or assessing a phased diploid assembly.
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Predicts protein-coding gene structures (exons, introns, UTRs) in eukaryotic genomes with BRAKER3 (RNA-seq + protein evidence), BRAKER1/BRAKER2, GALBA (protein-only), Funannotate (fungi), GeMoMa (homology projection), or Helixer/Tiberius (deep-learning ab initio). Covers the evidence-first tool decision, mandatory soft-masking, the training-set-quality-dominates principle, OrthoDB clade-partition selection, the one-isoform-per-locus and missing-UTR traps, merge/split errors, and reference bias against orphan genes. Use when annotating a newly assembled eukaryotic genome, choosing a gene-prediction pipeline based on available evidence, or diagnosing a poor annotation.
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Assembles haplotype-resolved diploid and telomere-to-telomere (T2T) genomes from PacBio HiFi reads with hifiasm (HiFi-only, Hi-C, or trio phasing) and verkko (HiFi + ultralong ONT for T2T), extracting contigs from GFA and routing phasing QC to k-mer/trio metrics. Covers why a primary assembly is a haplotype mosaic that exists in no cell, partial-vs-full phasing (the .bp. vs .dip. filename convention), the purge-default trap on inbred samples, the --hom-cov coverage-estimate alarm, and verkko-vs-hifiasm for T2T. Use when assembling a diploid eukaryote from HiFi, phasing haplotypes with parents (trio) or Hi-C, deciding whether to chase T2T, or diagnosing switch errors invisible to N50/BUSCO/QV.
日本語の概要は準備中です。原文の説明を表示しています。
Simulate transcription factor perturbation effects on cell state in silico with CellOracle and Dynamo, and predict transcriptional responses to genetic perturbations with GEARS, scGen, and CPA. Covers the direction-not-magnitude principle, local-linear validity, the GRN/velocity error it inherits, baseline discipline (mean and additive baselines), and the validation gap. Use when predicting TF knockout or overexpression effects, ranking driver TFs for fate transitions, or planning perturbation experiments. For GRN construction see multiomics-grn; for experimental Perturb-seq see single-cell/perturb-seq.
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Structures biological experiments so inference is valid by construction, covering Fisher's principles (randomization, replication, local control), the experimental-vs-observational unit distinction and pseudoreplication (Hurlbert 1984; Lazic 2018), randomization mechanics (complete, restricted, stratified, rerandomization, run-order), blocking layouts (randomized complete block, Latin square, incomplete block), factorial designs and interactions, and the split-plot/nested error strata hidden inside multi-batch genomics. Use when deciding the experimental unit and what counts as a replicate, planning randomization and run order, choosing a blocked/factorial/split-plot/nested layout, avoiding pseudoreplication in cell-culture or animal studies, or specifying the random-effects structure of the analysis model. For assigning samples to sequencing batches/lanes/plates and batch-effect correction see experimental-design/batch-design; for regulated clinical-trial randomization see clinical-biostatistics.
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Detect positive (diversifying / episodic / pervasive) selection using codon dN/dS frameworks. Implements PAML codeml site models (M0/M1a/M2a/M7/M8/M8a), branch models, branch-site model A (Zhang 2005), and HyPhy methods (BUSTED, BUSTED-S, BUSTED-MH, BUSTED-PH, MEME, FEL, FUBAR, aBSREL, SLAC, RELAX, GARD, FUBAR-MH). Includes McDonald-Kreitman framework (asymptotic alpha, impMKT, polyDFE, DFE-alpha, GRAPES) for within-species + divergence inference, RERconverge for trait-correlated rate shifts, CSUBST for convergent substitution, and PhyloAcc for accelerated noncoding evolution. Use when testing adaptive evolution at codons, branches, or full gene; running GARD recombination pre-screen; controlling alignment-error and gBGC false positives; reconciling PAML vs HyPhy results; or performing genome-scale selection scans.
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Use this skill when the user asks about driver discipline — when to coach vs warn vs suspend vs terminate, progressive discipline frameworks, at-will employment in trucking, documenting violations, separating "willful violation" from "human error," and how to handle a driver who refuses corrective action. Reference state employment law + ATA SMP.
日本語の概要は準備中です。原文の説明を表示しています。
Expert debugger for Next.js + Cloudflare Workers + Supabase stacks. Systematic troubleshooting for auth, caching, workers, RLS, CORS, and build issues. Activate on: 'debug', 'not working', 'error', 'broken', '500', '401', '403', 'cache issue', 'RLS', 'CORS'. NOT for: feature development (use language skills), architecture design (use system-architect).
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### 1. The Fault/Error/Failure Hierarchy NOT for unrelated tasks outside this domain.
日本語の概要は準備中です。原文の説明を表示しています。
Designing HTTP cache headers that work correctly across browsers, CDNs, and shared proxies — `Cache-Control` directives per RFC 9111, `stale-while-revalidate` and `stale-if-error` per RFC 5861, the Vary header for varying responses, and surrogate keys for tag-based purging. Grounded in IETF RFCs and Cloudflare/Fastly docs. NOT for service-worker or browser-only caching, Redis/memcached application caching, or edge-function compute.
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Interprets GitHub Actions run status and logs, diagnoses CI failure patterns, and suggests targeted fixes. Handles dependency install failures, test timeouts, build OOM, flaky tests, and workflow misconfigurations. Activate on: 'CI failing', 'build broken', 'workflow error', 'GitHub Actions debug', 'flaky CI', 'pipeline timeout', 'CI red'. NOT for: writing new workflows from scratch (use github-actions-pipeline-builder), CI caching strategy (use ci-cache-optimizer), deployment orchestration (use devops-automator).
日本語の概要は準備中です。原文の説明を表示しています。
Use when designing or fixing a Content Security Policy on a real site, choosing between nonce-based and hash-based CSP, adding strict-dynamic, debugging "Refused to execute inline script" errors, deploying CSP in report-only mode first, configuring report-to / report-uri, or auditing an existing policy for unsafe-inline / unsafe-eval / wildcards. Triggers: "CSP blocks legitimate inline script", strict-dynamic, nonce-{RANDOM}, sha256-{HASH}, object-src none, base-uri none, frame-ancestors, Trusted Types, X-Content-Security-Policy obsolete, report-only vs enforced. NOT for general HTTP security headers (HSTS, COOP/COEP), Trusted Types deep dive, CORS configuration, or building a WAF.
日本語の概要は準備中です。原文の説明を表示しています。
Identify and counteract cognitive biases in medical decision-making through systematic error analysis and contextual algorithm application. For diagnostic reasoning, treatment decisions, and clinical judgment improvement. NOT for basic medical knowledge, technical procedures, or non-clinical healthcare domains.
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Design and implement systematic checklists that reduce errors by 30-50% in complex, high-stakes domains (medicine, aviation, construction, finance). NOT for simple tasks or when comprehensive instruction is needed.
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Design decisions for when and what sounds to use in web apps, mobile apps, and desktop native apps — plus how to create them with AI. Covers UI sound vocabulary (confirms, alerts, transitions, errors), platform conventions (iOS, macOS, Material), creating sounds with AI tools (ElevenLabs, AudioCraft, Stable Audio), Web Audio API for procedural sounds, haptic-audio pairing, and the psychology of satisfying vs. annoying UI sounds. Activate on 'app sounds', 'UI sounds', 'notification sound', 'sound design app', 'earcons', 'sonic branding', 'interaction sounds', 'feedback sounds', 'app audio', 'satisfying sounds'. NOT for professional audio engineering (use sound-engineer), Windows 3.1 retro sounds specifically (use win31-audio-design), music composition (use sound-engineer).
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Produces a narrow-interval quality signal for a WinDAGs skill that a third party can verify without inspecting model weights or method internals. The mechanism is attribution-kNN over a tamper-evident outcome log (task→skills→accept/reject store), combined with a conformal-prediction calibration certificate that provides a formal coverage guarantee. Thompson/Beta sampling is explicitly rejected as a category error: it produces a selection signal for stationary i.i.d. rewards, not an attestation signal for context-dependent LLM output quality. The resulting bundle — outcome-log Merkle root + conformal threshold + optional TEE guardrail signature — constitutes a "narrow-interval trust" credential: verifiable, bounded, and internals-opaque.
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Design polished terminal UX for CLIs and TUIs. Use for setup flows, dashboards, tables, and actionable errors. NOT for web UIs, desktop windows, API schemas, or shell automation.
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Generates OpenAPI 3.0/3.1 specs, TypeScript client types, and curl examples from existing route handler code. Supports Express, Next.js API routes, Fastify, and Hono. Produces complete documentation including auth requirements, rate limits, error codes, and request/response examples. Activate on: 'generate API docs', 'OpenAPI from code', 'document endpoints', 'API reference', 'swagger generation', 'endpoint documentation'. NOT for: API design from scratch (use api-architect), deployment (use devops-automator), testing (use test-automation-expert).
日本語の概要は準備中です。原文の説明を表示しています。
Fundamental patterns for effective agentic behavior. Teaches decomposition, tool orchestration, error recovery, context management, quality self-assessment, and knowing when to stop. Model-agnostic principles that make any agent more effective regardless of domain. Activate on: "how should I structure this agent", "agentic workflow", "agent patterns", "multi-step task", "tool orchestration", "/agentic-patterns", "decompose this", "agent best practices", "chain of actions", "when should the agent stop", "agent loop design". NOT for: creating agent infrastructure (use agent-creator), building DAGs (use jury_rig-architect), specific tool implementation.
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Data structures and serialization formats for agent-to-agent communication. Covers message envelopes, structured output schemas, capability declarations, task handoff payloads, error/retry signaling, and context windows as data structures. Compares the version-pinned A2A, MCP, and JSON-RPC role boundaries covered by this bundle; OpenAI and LangChain-specific APIs require their own current sources. Teaches when to use rigid schemas versus free-form content with validation, typed versus untyped boundaries, and streaming versus batch. Activate on: "agent message format", "agent communication schema", "agent-to-agent protocol", "A2A protocol", "MCP message format", "structured output for agents", "agent interop", "interchange format", "agent serialization", "task handoff format", "capability declaration". NOT for: what agents say to each other (use agent-conversation-protocols), orchestration topology (use multi-agent-coordination), building agent infrastructure (use agentic-infrastructure-2026).
日本語の概要は準備中です。原文の説明を表示しています。