「lia」の検索結果

Design roadside signs and large-format ads for businesses, especially automotive dealers. Use for pylon signs, monument signs, lot banners, highway billboards, digital roadside boards, service-lane signage, used-car event signs, lease or finance campaign concepts, and other driver-at-speed messaging where legibility, offer hierarchy, and dealer ad compliance matter. Activate on "road sign", "billboard", "dealership sign", "car dealer ad", "pylon sign", "monument sign", "service lane signage", "automotive billboard", "roadside ad", "dealer lease offer", or "drive-by signage". NOT for fabrication drawings, permitting or local sign-code review, web or social ad campaigns, or full brand identity systems.

日本語の概要は準備中です。原文の説明を表示しています。

Creates new skills with proper structure, compliance checks, and registration. Enforces mk: prefix, sub-agents.md structure, and context-engineering principles. Use to scaffold a new skill.

日本語の概要は準備中です。原文の説明を表示しています。

Create new skills with proper structure, compliance checks, and registration. Use when asked to create a skill, build a new skill, or scaffold a skill template. Enforces mk: prefix, sub-agents.md structure, and context engineering principles.

日本語の概要は準備中です。原文の説明を表示しています。

Use when designing UI components, creating design systems, reviewing visual design, or checking accessibility compliance. Triggers on "design system", "UI review", "color palette", "font pairing", "accessibility audit", "responsive design", "WCAG check". Auto-activates when ui-ux-designer agent is working. Data-driven: 160 palettes, 73 font pairings, 161 product types, 98 UX guidelines, 25 chart types.

日本語の概要は準備中です。原文の説明を表示しています。

Test-to-requirement coverage mapping. Reads plan acceptance criteria and test files, produces a coverage gap report showing which requirements have no tests. Use during Phase 2 (Test) or Phase 4 (Review) to verify test completeness. Named after the Nyquist sampling theorem — sufficient test coverage prevents aliased (missed) requirements. NOT for running tests (see mk:testing); NOT for structural code review (see mk:review).

日本語の概要は準備中です。原文の説明を表示しています。

Design and implement backend service changes: API contracts, service boundaries, auth integration, reliability and delivery checks. Use for backend/API handlers, services, webhooks, jobs, or backend architecture changes. Routes schema/migrations to mk:database; security review to the existing security workflow.

日本語の概要は準備中です。原文の説明を表示しています。

Plan and implement infrastructure, containers, CI/CD, deployment safety, observability, and rollback changes. Use for Docker, IaC, cloud runtime, Kubernetes, pipeline, deployment, or operational reliability work. Does not read dotenv files or approve/release deployments.

日本語の概要は準備中です。原文の説明を表示しています。

Alias for sdd-dev — Spec-Driven Development workflow

日本語の概要は準備中です。原文の説明を表示しています。

Alias for sdd-dev — Spec-Driven Development workflow

日本語の概要は準備中です。原文の説明を表示しています。

Create interactive AI role-play training activities as self-contained HTML. Use when the user asks to generate a role-play, persona practice, sales simulation, customer conversation drill, compliance scenario, or any learner-vs-AI-character training exercise.

日本語の概要は準備中です。原文の説明を表示しています。

Digital signatures and time stamps through Openapi - eIDAS-compliant electronic signatures (SES with OTP, qualified QES, e-seals, massive automatic signing) and qualified time stamping of documents. Use when the user needs a document signed electronically or time-stamped with legal validity.

日本語の概要は準備中です。原文の説明を表示しています。

Italian electronic invoicing and payments through Openapi - send/receive electronic invoices via SDI (Sistema di Interscambio), digital receipts (scontrino elettronico), legal storage, and bill payments (MAV, RAV, PagoPA, car tax). Use for fatturazione elettronica and bill payment tasks.

日本語の概要は準備中です。原文の説明を表示しています。

Look up company/business data through Openapi - Italian companies by VAT/tax code or name, European and worldwide company search, shareholders, UBO, AML, PEC/SDI codes, company monitoring. Use for questions like "find data about company X", "who owns VAT number Y", "search companies in France/EU/worldwide".

日本語の概要は準備中です。原文の説明を表示しています。

Assess the reliability of companies and individuals through Openapi Risk - credit scores, CRIF reports, patrimonial reports, negative events, heirs reports, worldwide KYC (PEP, sanction lists, adverse media). Use for due diligence, creditworthiness, AML/KYC questions.

日本語の概要は準備中です。原文の説明を表示しています。

Map unfamiliar code or research an approach before planning, read-only and fast.

日本語の概要は準備中です。原文の説明を表示しています。

Calls m6A peaks from MeRIP-seq / m6A-seq paired IP-vs-input data using exomePeak2 (transcript-aware, GC-bias-corrected Poisson GLM), MeTPeak (HMM over sliding windows), MACS3/MACS2 with --nomodel --broad --keep-dup all (genome-wide broad alternative), and DRACH motif enrichment via HOMER or ggseqlogo as a sanity check (NOT a filter). Covers BED12 vs narrowPeak output, exonic vs intronic peak handling, multi-tool reconciliation (intersection vs union), the m6A-vs-m6Am ambiguity at 5'UTR peaks that antibody methods cannot resolve, and orthogonal validation (miCLIP/GLORI/m6A-SAC-seq/m6Anet). Use when calling peaks from paired IP/input genome BAMs, choosing exomePeak2 (transcript-aware default) vs MACS3 (broad genomic) vs MeTPeak (HMM-smoothed low-coverage), confirming DRACH enrichment as a sanity check on the peak set, reconciling differing peak sets across tools, validating MeRIP peaks against single-base methods, interpreting 5' peaks where m6Am contamination is possible, or recommending a consensus strategy.

日本語の概要は準備中です。原文の説明を表示しています。

Maps between gene identifier systems (Ensembl, Entrez, HGNC symbol, UniProt, RefSeq, MANE) using AnnotationDbi, biomaRt, mygene, pyensembl, and Ensembl REST. Encodes Ensembl version stripping with GENCODE _PAR_Y preservation, the Ziemann 2016 Excel autocorrect debacle and Bruford 2020 HGNC renames (SEPT*->SEPTIN*, MARCH*->MARCHF*, MARC*->MTARC*, DEC1->DELEC1), OCT4/POU5F1 alias resolution, biomaRt archive endpoints for release pinning, the `filters` (plural) gotcha, MANE Select for clinical reporting, cross-species orthology via Ensembl Compara / OMA / OrthoDB, and tx2gene construction for tximport. Use when converting gene IDs across systems, handling renamed symbols, building tx2gene, pinning to a specific Ensembl release for reproducibility, or mapping cross-species orthologs.

日本語の概要は準備中です。原文の説明を表示しています。

Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.

日本語の概要は準備中です。原文の説明を表示しています。

Assigns pathogen lineages (SARS-CoV-2 Pangolin UShER mode; Nextclade clade + QC; pango-designation alias resolution) and tracks variant frequencies over time using Nextstrain (Augur + Auspice), wastewater deconvolution (Freyja, COJAC, alcov, lineagespot), lineage-fitness modelling (multinomial logistic), and recombinant detection (3SEQ, RDP4, Bolotie). Covers Pangolin pangolin-data and Nextclade dataset version pinning (mandatory; lineage-defining mutations change with dataset), Freyja barcode forward-only date constraint, ARTIC primer scheme churn (V3/V4/V4.1/V5.3.2/Midnight) with dropout regions, and recombinant X-prefix designation lag. Use when assigning Pango lineages and Nextclade clades to viral consensus sequences, building Nextstrain Augur surveillance pipelines, deconvolving wastewater into lineage frequencies with Freyja, tracking lineage frequencies over time, handling ARTIC primer dropouts, or running surveillance for SARS-CoV-2/influenza/Mpox/RSV/H5N1/measles.

日本語の概要は準備中です。原文の説明を表示しています。

Designs pooled sgRNA libraries for CRISPR knockout, interference (CRISPRi), activation (CRISPRa), Cas12a multiplex, base-editor, and prime-editor screens. Covers on-target scoring (Rule Set 2, Azimuth, DeepSpCas9, CRISPRon), off-target scoring (CFD, MIT), TSS-relative positioning for CRISPRi/a (Horlbeck, Dolcetto, Calabrese), PAM-variant chemistries, control-guide composition, oligo cloning architecture, and library QC. Use when choosing a genome-wide library (GeCKOv2 vs Avana vs Brunello vs TKOv3 vs Inzolia), designing a focused or paralog-focused custom library, picking CRISPRi vs CRISPRa TSS windows, deciding control-guide proportions, or diagnosing library skew and dropout in a freshly cloned pool.

日本語の概要は準備中です。原文の説明を表示しています。

Model gene-family birth-death dynamics across a species tree using CAFE5 (Mendes et al 2020 Bioinformatics 36:5516 gamma-distributed rate categories), CAFE5-error (annotation-error-aware), Count (Csurös 2010 ancestral state reconstruction), BadiRate (Librado 2012 likelihood + parsimony), DupliPHY-Family, and ALE/AleRax (for per-family DTL; see [[gene-tree-species-tree-reconciliation]]). Test lineage-specific gene-family expansions and contractions, distinguish biological dynamics from annotation artifacts, account for assembly fragmentation, identify functional enrichment in expanded / contracted families. Use when correlating gene-family changes with phenotype evolution, ranking lineages by adaptive gene-family-rate shifts, post-WGD dosage-balance analysis, or building Birth-death models from OrthoFinder presence/absence matrices.

日本語の概要は準備中です。原文の説明を表示しています。

Infer integer allele-specific copy number, tumor purity, and ploidy from tumor sequencing by jointly modeling read depth (logR) and B-allele frequency (BAF) with ASCAT, Sequenza, FACETS, PURPLE, and PureCN (tumor-only). Covers the purity-ploidy identifiability problem, the diploid-baseline (dipLogR) anchor, major/minor copy number, loss of heterozygosity, sunrise/contour fit diagnostics, and reconciliation of conflicting fits. Use when tumor analysis needs absolute copy number rather than relative log2, when estimating purity and ploidy, calling LOH or copy-neutral LOH, resolving whole-genome doubling, running tumor-only allele-specific calling, or choosing among ASCAT, Sequenza, FACETS, and PureCN.

日本語の概要は準備中です。原文の説明を表示しています。

Prioritizes rare-disease variants from trio/quad WES/WGS with de novo (DeNovoGear, Triodenovo), compound-heterozygous phasing (WhatsHap), mosaic VAF tiering, phenotype-driven ranking (Exomiser, Phen2Gene, AMELIE), ClinGen gene-disease validity gating, and ACMG SF v3.2 secondary findings reporting. Use when running diagnostic exome / genome pipelines, identifying candidate Mendelian disease genes, screening for incidental findings, or auditing VUS reclassification cycles. The ACMG/AMP classification framework (PVS1 decision tree, Pejaver PP3/BP4 calibration, Tavtigian point system) is in clinical-databases/acmg-classification.

日本語の概要は準備中です。原文の説明を表示しています。

Comprehensive quality control for CLIP-seq libraries (eCLIP, iCLIP, iCLIP2, PAR-CLIP) covering library complexity (preseq), FRiP, IDR replicate reproducibility, read-distribution metagene, SMInput vs IgG control rationale, rRNA / snoRNA contamination, fragment-length distribution, and ENCODE-compliance thresholds. Use when assessing whether a CLIP library passed, deciding lenient vs stringent peak thresholds, comparing replicates with IDR rescue and self-consistency ratios, or distinguishing failed IP from over-amplified library.

日本語の概要は準備中です。原文の説明を表示しています。