563 件 ・ 関連度順
概要と使いどころ
CLI toolkit for SAM/BAM/CRAM: sort, index, convert, filter, QC alignments. Core commands: view, sort, index, flagstat, stats, depth, markdup, merge. Required between alignment and variant/peak calling. Use pysam for Python-native BAM access; deeptools for normalized coverage tracks.
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Poisson-model peak caller for ChIP-seq/ATAC-seq BAMs. MACS3 callpeak finds enriched regions (TF sites or histone marks) vs input/IgG; outputs BED narrowPeak/broadPeak for motif analysis, annotation, and differential binding. Use narrow peaks for TF ChIP-seq and ATAC-seq; broad for H3K27me3, H3K9me3, and other broad marks.
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Provides patterns to build declarative AI Services with LangChain4j for LLM integration, chatbot development, AI agent implementation, and conversational AI in Java. Generates type-safe AI services using interface-based patterns, annotations, memory management, and tools integration. Use when creating AI-powered Java applications with minimal boilerplate, implementing conversational AI with memory, or building AI agents with function calling.
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Use when querying or calling pay-per-token foundation models, or when creating, updating, listing, or querying Unity Gateway (also called Unity AI Gateway) services, including model provider services, MCP services, system.ai services, and three-part Unity Catalog service names (catalog.schema.service). Also use for managing spend budgets and usage alerts on Unity Gateway, and for migrating from legacy AI Gateway. Also use when a workload with Model Serving endpoint permissions (for example CAN_QUERY) gets PERMISSION_DENIED querying a three-part model name, since serving-endpoint access does not grant access to a Unity Gateway service. Not for configuring legacy AI Gateway on Model Serving endpoints; use databricks-model-serving for that.
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What-if scenario analyzer for sports. Play-calling recommendations, clock management, substitution patterns, risk/reward calculations.
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Use DeepVariant and DeepTrio for genomics variant-calling command planning, input validation, pipeline-stage troubleshooting, custom training, and report analysis.
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Show per-asset vuln-class coverage gaps for the active engagement so nothing in scope is skipped. Use when asked "coverage", "what haven't we tested", "test gaps", "are we thorough", or before calling an engagement done.
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Agnes AI API 接入支持与问题排查 Skill。帮助新用户完成 Agnes AI API 的接入配置， 诊断和解决接入过程中遇到的认证、参数、响应、图像生成、视频生成等各类问题。 涵盖 Thinking 模式开启、视频长度调节、流式输出、工具调用等高级功能指导。 兼容 OpenClaw、Claude Code、Hermes、Codex 等工具链。 thinking、stream、tool calling、模型选择、Base URL、响应格式、timeout、超时、轮询、 FAQ、问题排查、调试、免费调用、RPM、video_id、task_id、Prompt 模板、提示词、 生图、生视频、图生图、文生图、图生视频、文生视频、灰度自检、1M 上下文、4K 图片、4K 图、4K、灰度、自检、反馈、Bug、issue、问题反馈、功能建议。
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Use when calling external HTTP APIs from Spring Boot 4 / Spring Framework 7 with declarative HttpExchange interfaces. Covers ImportHttpServices, grouped base URLs and timeouts, and RestClient versus WebClient selection.
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Use when calling external HTTP APIs from Spring Boot 3 / Spring Framework 6 with declarative HttpExchange interfaces. Covers manual proxy registration, RestClient versus WebClient, and timeout and error handling conventions.
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OI-3 spike harness — the INLINE (non-forked) readability-guidance variant. Surfaces the shared readability standard into the calling skill's own context. Use only when a spike consumer skill invokes it.
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Surfaces Han's shared Human-Readable Output Standard — the readability rule and the writing-voice profile — into the calling skill's own context, so the caller drafts in voice and runs its self-check against the current standard sourced from one canonical copy. Use when a prose-producing skill needs the shared readability standard available in context before it drafts. Governs the shape of a written deliverable, where explanation-guidance governs what a run says to a person in a turn. Runs in the caller's context and hands control straight back; it does not produce a deliverable of its own, rewrite anything, or judge the caller's work. Does not run the adversarial rewrite pass — dispatch the readability-editor agent for that, or use edit-for-readability to rewrite an existing target. Does not cover explaining technical work to a reader who will not implement it — use explanation-guidance for that.
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Surfaces Han's shared standard for explaining technical work to a reader who will not implement it into the calling skill's own context, so the caller writes its escalations, confirmation turns, and stops as a concrete outcome the reader could observe rather than as a mechanism. Use when a skill is about to ask a person a question, stop for an input, or explain a technical consequence to someone who will not open the code. Governs what a run says to a person in a turn, where readability-guidance governs the shape of a written deliverable. Runs in the caller's context and hands control straight back; it does not produce a deliverable of its own, rewrite anything, or judge the caller's work. Carries guidance only and adds no self-check step.
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Build DSPy evaluation harnesses with rich-feedback metrics that are essential for GEPA optimization. Use when writing a metric function, calling dspy.Evaluate, splitting dev/val sets, debugging "why is my optimizer not improving?", or designing CI-ready DSPy eval suites.
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Fix Riverpod "Using ref when widget is unmounted is unsafe" error in ConsumerStatefulWidget. Use when: (1) Error thrown in dispose() when calling ref.read(), (2) Need to call a service method during widget cleanup, (3) ConsumerStatefulWidget needs to perform actions on provider when user navigates away. Solution: cache the service/notifier in initState with late final.
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Fix "Requested backend named 'X' does not exist" or TLS alert errors in Fastly Compute@Edge. Use when: (1) Backend works locally but fails in production with "backend does not exist", (2) TLS alert received (alert_id=0) errors when calling external APIs from Fastly, (3) Backends defined in fastly.toml aren't available after deployment. The fastly.toml [[backends]] section only applies during initial service creation - existing services require backends to be added via CLI or API with proper SSL/SNI configuration.
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Fix Dart/Flutter services where calling start() after stop() is a silent no-op because stop() sets a _disposed (or similar) flag that start()'s guard short-circuits on. Use when: (1) A repository/service/controller has startListening/stopListening, subscribe/unsubscribe, open/close, or similar lifecycle methods, (2) Re-opening the service after closing it appears to do nothing — no subscription, no events, no error, (3) Unit tests that only exercise a single mount/open/start cycle pass while the real app breaks on the second visit to a screen, (4) A boolean flag is used both for "in the middle of tearing down this instance forever" AND for "currently stopped, can be re-started". Common in Riverpod/Bloc-driven screens that wire startListening() in initState and stopListening() in dispose — the second time the user visits the screen, nothing happens.
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Find the papers that answer a research query in Firecrawl's research paper index — a corpus of paper abstracts whose largest share is biomedical and life-science literature (PubMed, bioRxiv, medRxiv), alongside arXiv preprints in CS, physics, and math — using semantic search, semantic and structural expansion, and in-body verification. Use this skill for literature-finding and paper-retrieval tasks of any kind, including clinical, biomedical, drug, gene, disease, and other life-science questions, whether the answer is a single paper or a full multi-paper set. The index is reached only through the `firecrawl_research_*` MCP tools or the `firecrawl research` CLI subcommands. Calling `firecrawl_search` with its `categories` option set to `["research"]` is a different feature — it filters ordinary web search to research-affiliated websites (the list includes PubMed, bioRxiv, medRxiv, arXiv, and publisher sites) and returns page results from them, without querying the paper records in this index.
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> Generate, modify, and style React components from natural language using the Thesys SDK. Guides schema-driven UI generation, theme customisation, tool calling integration, and deployment to Vite, Next.js, or Cloudflare Workers. Use when the user says "generate UI", "create a component", "build an interface", "Thesys", "generative UI", or asks to turn a description into a React component.
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Run a one-shot or flat parallel batch of provider LLM subagents (headless `cc-fleet subagent`) that return a result. Use when fanning out N independent tasks, doing bulk per-file work, or calling a specialized provider model (DeepSeek / GLM / Kimi / Qwen / MiniMax). NOT a long-lived collaborator you message back and forth (that is /cc-fleet:team); NOT a multi-phase pipeline with dependencies or resume (that is /cc-fleet:workflow); NOT trivial work the main session should just do.
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当需要为 AI 定义工具接口、设计调用规范、实现工具发现与编排机制时调用此 skill。典型场景包括：设计 AI agent 的工具集、定义 JSON Schema/XML/TypeScript 格式的工具描述、实现工具权限控制与并行调度、设计子代理委托架构。 不适用于：定义 AI 身份（persona-design）、安全规则（safety-guardrails）、记忆存储（memory-system）。 关键 trigger 信号：AI 需要调用外部 API 或服务、工具数量超过 10 个需要发现机制、需要区分工具权限层级、需要子代理并行执行、工具调用涉及敏感操作需要审批。
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Design and validate model-facing tool definitions with clear names, action-oriented descriptions, bounded JSON Schema parameters, explicit side effects, safe defaults, idempotency, errors, and realistic tests. Use when creating function-calling tools, MCP tools, agent actions, structured tool inputs, or when a model selects the wrong tool, invents arguments, or causes unsafe side effects.
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Think and work like an expert Animal Geneticist & Breeder. Use when a task calls for Animal Geneticist & Breeder judgment. Reasons from additive genetic variance, response to selection (R = i h sigma_A), accuracy, and inbreeding depression through REML/BLUP and ssGBLUP pipelines (BLUPF90, ASReml, WOMBAT), economic selection indices, optimum-contribution mate allocation, and Interbull MACE while treating confounded contemporary groups, prediction bias/dispersion, popular-sire inbreeding and rising deleterious haplotypes, GxE reranking, and pedigree or genotype-calling errors as first-class failure modes.
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Build production OpenAI Realtime voice agents and low-latency spoken interactions with live audio sessions, WebRTC or WebSocket transport, voice activity detection, tool/function calling, prompt design, logging, consent, privacy, safety, latency, and cost controls. Use for speech-to-speech agents and live voice UX; do not use for separate request-based transcription, text-to-speech, or offline audio generation work.
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