「issue」の検索結果

Segment single cells from multiplexed IMC/MIBI tissue images using Mesmer/DeepCell, Cellpose, or ilastik+CellProfiler, covering whole-cell vs nuclear segmentation, the summed-membrane-channel decision, nuclear-expansion bias, lateral spillover, resolution-floor parameters, and downstream-proxy evaluation. Use when delineating cells after preprocessing, choosing a segmentation model, building a cell mask for quantification, diagnosing impossible double-positive populations, or troubleshooting over/under-segmentation.

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Calculates tumor mutational burden from WES/WGS/panel data with Friends of Cancer Research harmonization equations, per-assay calibration (FDA 10/Mb = 7.8 TSO500 = 8.4 OncomineTML), synonymous/indel/germline filtering, hypermutator tiering, blood TMB, and integration with HLA-LOH and neoantigen quality (Luksza 2017 fitness). Use when assessing ICI eligibility under tumor-specific cutoffs (McGrail 2021), comparing tissue vs bTMB, or auditing TMB-H reporting against ESMO 2024 and FDA pembrolizumab pan-tumor 2020.

日本語の概要は準備中です。原文の説明を表示しています。

Estimate SNP heritability and partition it across functional annotations, cell types, and loci from GWAS summary statistics or individual-level genotypes. Implements LDSC, stratified LDSC with the baseline-LD model, Finucane 2018 cell-type prioritization, LDAK SumHer, HDL, HESS local heritability, BOLT-REML, GCTA-GREML, graphREML, and Popcorn cross-population genetic correlation. Use when computing total h2_SNP from summary stats, partitioning heritability across functional categories, prioritizing trait-relevant tissues or cell types from ENCODE/Roadmap chromatin marks, reconciling LDSC vs LDAK enrichment estimates, computing local heritability with HESS, estimating genetic correlation between traits, or producing publication-grade enrichment with calibrated sensitivity to model assumptions.

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Maps GWAS-implicated loci to candidate effector (causal) genes by integrating variant-to-gene (V2G) features via Open Targets L2G (Mountjoy 2021), MAGMA gene-based association (de Leeuw 2015), FUMA SNP2GENE, cS2G combined SNP-to-gene scores (Gazal 2022), Polygenic Priority Scores (PoPS, Weeks 2023), FLAMES, INQUISIT, DEPICT, and enhancer-gene predictors (ABC, ENCODE-rE2G). Use when narrowing a GWAS lead locus to a candidate causal gene, picking between proximity, eQTL-based, and similarity-based prioritizers, integrating multi-evidence streams (fine-mapping, colocalization, ABC enhancer-gene, distance, chromatin), reconciling discordant L2G vs PoPS calls, prioritizing tissue-specific eQTL evidence, or triangulating across at least three independent lines of evidence for a publication-grade effector-gene nomination.

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Detects aberrant splicing in single rare-disease patients vs a control panel using FRASER 2.0 (Bioconductor; Beta-binomial autoencoder on Intron Jaccard Index, default delta cutoff 0.1, q hyperparameter), OUTRIDER (gene-level outlier expression via autoencoder denoising), LeafcutterMD (Dirichlet-multinomial outlier mode of LeafCutter for annotation-free junctions), and DROP (Snakemake pipeline integrating FRASER2 + OUTRIDER + monoallelic expression for clinical diagnostics). The statistical model is fundamentally different from differential splicing — single-sample-vs-cohort outlier detection rather than two-group comparison. Standard tool in EU rare-disease (Solve-RD) and NIH UDN programs. Use when applying RNA-seq to undiagnosed Mendelian disease, validating predicted splice variants in clinical samples, or detecting cryptic splicing in disease tissue.

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Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.

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Run build, deploy, inspect, and fix loops for .NET MAUI apps that already have MAUI DevFlow integrated. USE FOR: launching MAUI apps, selecting devices or emulators, waiting for or recovering agent connections, broker/port/adb connectivity issues, visual tree inspection, screenshots, UI interaction, Blazor WebView CDP debugging, reading DevFlow logs, and iterative app debugging. DO NOT USE FOR: first-time DevFlow package setup (use maui-devflow-onboard), or generic desktop automation unrelated to MAUI. INVOKES: maui devflow CLI, dotnet CLI, Android adb/android tools, and Apple simctl tools.

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Use this skill for wheel-end maintenance and lubrication. Covers oil-bath vs grease packs, wheel-end loss issues, and inspection intervals.

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Use this skill when buying a used tractor or trailer. Covers verifying compliance status, hidden issues, and what to inspect before signing.

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Use this skill when a driver holds CDLs in multiple states or has resided in multiple states. Covers what MVRs to pull, federal recordkeeping requirements, and state-specific issues.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to verify that a driver's medical certificate was issued by a certified examiner on the FMCSA National Registry. Covers the search process, what to verify, and how to handle examiner removal.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about CDL driver wellness, retention strategies, what causes driver turnover, common health issues (obesity, hypertension, sleep apnea, mental health), benefits packages, home-time policies, and how to build a culture that keeps drivers. Reference ATA Workforce Development + FMCSA Wellness Initiative.

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Technical SEO audit across 9 categories: crawlability, indexability, security, URL structure, mobile, Core Web Vitals, structured data, JavaScript rendering, and IndexNow protocol. Use when user says "technical SEO", "crawl issues", "robots.txt", "Core Web Vitals", "site speed", or "security headers".

日本語の概要は準備中です。原文の説明を表示しています。

Analyze existing XML sitemaps or generate new ones with industry templates. Validates format, URLs, and structure. Use when user says "sitemap", "generate sitemap", "sitemap issues", or "XML sitemap".

日本語の概要は準備中です。原文の説明を表示しています。

iOS-native mobile web UX rules. Use when building any UI component, fixing mobile issues, reviewing responsive code, or touching viewport heights (dvh vs h-screen), safe areas, Dialog vs Drawer, tabs on mobile, touch targets, or input zoom.

日本語の概要は準備中です。原文の説明を表示しています。

The re-measure and standing report of the canonical data model's live DB conformance. Use when asked 'where are we at' on DB drift or conformance, to update the drift numbers, when a drift-audit brief is re-issued, or on /drift-audit. NOT the per-table fix recipe (use matrx-frontend's db-canonicalize-table).

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Create a new find-XXXX preprocessor Python script from scratch (no existing SKILL.md), add configs/<GAMEVER>.yaml skill and symbol entries. Covers xref-string-based and LLM_DECOMPILE-based discovery patterns. Use when a GitHub issue or user instruction specifies a new function to find.

日本語の概要は準備中です。原文の説明を表示しています。

One-click contribution flow for Open Design (nexu-io/open-design) — even for non-coders. Pick one of four cards (ship a Skill or Design System you made with OD; translate docs; fix a typo / write a blog; report a bug), the agent validates and opens a PR (or issue) for you. Trigger words contribute to open design, ship my OD skill, ship my OD design system, translate OD docs, report an OD bug, od-contribute.

日本語の概要は準備中です。原文の説明を表示しています。

Use when the user asks to inspect Sentry issues or events, summarize recent production errors, or pull basic Sentry health data via the Sentry API; perform read-only queries with the bundled script and require `SENTRY_AUTH_TOKEN`.

日本語の概要は準備中です。原文の説明を表示しています。

CSS and UI animation patterns for responsive, polished interfaces. Use when implementing hover effects, tooltips, button feedback, transitions, or fixing animation issues like flicker and shakiness.

日本語の概要は準備中です。原文の説明を表示しています。

Manage issues, projects & team workflows in Linear. Use when the user wants to read, create or updates tickets in Linear.

日本語の概要は準備中です。原文の説明を表示しています。

Access Google Search Console for SEO data such as queries, clicks, impressions, rankings, and indexing status across sites. Use when diagnosing search performance or indexing issues.

日本語の概要は準備中です。原文の説明を表示しています。

When the user wants to add, fix, or optimize schema markup and structured data on their site. Also use when the user mentions "schema markup," "structured data," "JSON-LD," "rich snippets," "schema.org," "FAQ schema," "product schema," "review schema," or "breadcrumb schema." For broader SEO issues, see seo-audit.

日本語の概要は準備中です。原文の説明を表示しています。

Run after making React changes to catch issues early. Use when reviewing code, finishing a feature, or fixing bugs in a React project.

日本語の概要は準備中です。原文の説明を表示しています。