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Analyzes base-editing screens for variant function. Covers library design (Sanson 2020 GRACE, Hanna 2021 BRCA1/2 SNV scanning, Cuella-Martin 2021), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end, wider for ABE8e), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

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Build interactive HTML/web visualizations with plotly (Python/R), bokeh (Python), and gganimate/plotly frames for animation, with awareness of current Kaleido static-export model (post-orca-EOL), HTML file-size bloat, and the limits of interactive-only output for journal submission. Use when producing zoomable/hoverable plots for notebook EDA, supplementary HTML, dashboards, or animated time-course / iteration visualizations.

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Call copy number variants with the GATK best-practices workflows — the somatic CNV pipeline (CollectReadCounts, DenoiseReadCounts with tangent normalization, ModelSegments, CallCopyRatioSegments) and the germline GATK-gCNV pipeline (DetermineGermlineContigPloidy, GermlineCNVCaller cohort/case mode, PostprocessGermlineCNVCalls). Covers panel-of-normals construction, AnnotateIntervals/FilterIntervals, allelic-count integration, and QS-based filtering. Use when integrating CNV calling into a GATK variant pipeline, calling rare germline CNVs from an exome cohort, deciding between the somatic and germline GATK workflows, or diagnosing why tangent normalization removed a real event or why gCNV output has low precision.

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The provision for a call site: the exhaustive menu of values that place in the code can realistically produce. Use before creating or fixing a mandate or agent that reasons about more than its own input, when deciding whether an agent can answer at all with what it is sent, or when its output 'looks right' but nobody checked it could be. NOT for building the agent (use create-agent).

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Custom chat display for an MCP or agent tool's result, DB-loaded from tool_ui by default. Use when adding or customizing a tool visualization, making a tool result look good, rendering MCP tool output, or authoring a renderer for a tool that shows the generic view.

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A live demo or admin page shipped with every feature whose results the user would otherwise not see. Use when adding a backend endpoint or feature with no UI to see it, when output only shows in logs, the DB, or an agent run, or when the user asks 'how do I test this'.

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Locate CCSPlayer_MovementServices::Pawn in CS2 server.dll / libserver.so via IDA Pro MCP and emit a fresh, minimal-unique signature or offset for the local gamedata entry "CCSPlayer_MovementServices::Pawn" (symbol CCSPlayer_MovementServices_Pawn). This is a STRUCT MEMBER OFFSET (schema netvar), not a function. Resolve the CCSPlayer_MovementServices class layout via the schema/network system; the member must satisfy natural alignment. Cross-check with functions that dereference the member. Do not switch output schema: emit the structmember fields below even if the class turns out to be polymorphic - a func_*/vfunc_* payload for this symbol is rejected by canonical_symbol_yaml_bytes. Trigger: CCSPlayer_MovementServices_Pawn, CCSPlayer_MovementServices::Pawn

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Locate CCSBot::Profile in CS2 server.dll / libserver.so via IDA Pro MCP and emit a fresh, minimal-unique signature or offset for the local gamedata entry "CCSBot::Profile" (symbol CCSBot_Profile). This is a STRUCT MEMBER OFFSET (schema netvar), not a function. Resolve the CCSBot class layout via the schema/network system; the member must satisfy natural alignment. Cross-check with functions that dereference the member. Do not switch output schema: emit the structmember fields below even if the class turns out to be polymorphic - a func_*/vfunc_* payload for this symbol is rejected by canonical_symbol_yaml_bytes. Trigger: CCSBot_Profile, CCSBot::Profile

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Use when designing or improving prompts, commands, hooks, skills, or sub-agent instructions, including output quality, reliability, and token efficiency.

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Elite frontend image-direction skill for generating premium, conversion-aware website design references. CRITICAL OUTPUT RULE — generate ONE separate horizontal image FOR EVERY section. A landing page with 8 sections produces 8 images. Never compress multiple sections into one image. Enforces composition variety (not always left-text / right-image), background-image freedom, varied CTAs, varied hero scales (giant / mid / mini minimalist), narrative concept spine, second-read moments, and a single consistent palette across all images. Optimized for landing pages, marketing sites, and product comps that developers or coding models can accurately recreate.

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Generates code examples for PHP documentation. Creates minimal, copy-paste ready examples with expected output.

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Proactively validates Claude Code's own generated HTML/JSX/TSX output for accessibility before presenting to users. Use this skill automatically when generating UI code to ensure WCAG 2.1 AA compliance.

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[51] EXECUTE. Commitment to maximum quality work with 150% coverage. Use when you need the highest quality output for critical tasks, complex problems, important decisions, or when standard work isn't enough. Triggers on "maximum quality", "150% mode", "full quality", "critical task", or when you explicitly want AI to work at its best.

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Analyze raw prompts, identify intent and gaps, match ECC components (skills/commands/agents/hooks), and output a ready-to-paste optimized prompt. Advisory role only — never executes the task itself. TRIGGER when: user says "optimize prompt", "improve my prompt", "how to write a prompt for", "help me prompt", "rewrite this prompt", or explicitly asks to enhance prompt quality. Also triggers on Chinese equivalents: "优化prompt", "改进prompt", "怎么写prompt", "帮我优化这个指令". DO NOT TRIGGER when: user wants the task executed directly, or says "just do it" / "直接做". DO NOT TRIGGER when user says "优化代码", "优化性能", "optimize performance", "optimize this code" — those are refactoring/performance tasks, not prompt optimization.

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Engineering operating model for teams where AI agents generate a large share of implementation output.

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Algorithmic philosophies are computational aesthetic movements that are then expressed through code. Output .md files (philosophy), .html files (interactive viewer), and .js files (generative algorithms).

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Actorization converts existing software into reusable serverless applications compatible with the Apify platform. Actors are programs packaged as Docker images that accept well-defined JSON input, perform an action, and optionally produce structured JSON output.

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This skill should be used when the user asks to "implement LLM-as-judge", "compare model outputs", "create evaluation rubrics", "mitigate evaluation bias", or mentions direct scoring, pairwise comparison, position bias, evaluation pipelines, or automated quality assessment.

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Design spec with 98 rules for building CLI tools that AI agents can safely use. Covers structured JSON output, error handling, input contracts, safety guardrails, exit codes, and agent self-description.

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Runs the morning maintainer standup for NemoClaw. Triages the backlog, determines the day's target version, labels selected items, surfaces stragglers from previous versions, and outputs the daily plan. Use at the start of the workday. Trigger keywords - morning, standup, start of day, daily plan, what are we shipping today.

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Use this skill when importing Excel, CSV, or pasted rows with nuxt-ui-tools as a package consumer. Covers defineSpreadsheetSchema (typed context, chained text/number/date/boolean/select columns, columns depending on the columns above (options, defaults, rules per row), groups and dynamic groups built from the context, exact matching, option sources shared with forms, unknown-value policies, rules and levels, validate, row identity with duplicates and stored records, output), useSpreadsheetImport (headless models, onSubmit with batches and rejections), the UiSpreadsheetImport* parts, useSpreadsheetSteps with custom steps, and the ready-made UiSpreadsheetImport wizard.

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Guarantee valid JSON/XML/code structure during generation, use Pydantic models for type-safe outputs, support local models (Transformers, vLLM), and maximize inference speed with Outlines - dottxt.ai's structured generation library

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Remote-control tmux sessions for interactive CLIs by sending keystrokes and scraping pane output.

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Control LLM output with regex and grammars, guarantee valid JSON/XML/code generation, enforce structured formats, and build multi-step workflows with Guidance - Microsoft Research's constrained generation framework

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