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Knowledge base from the FAA System Safety Handbook (Dec 30, 2000). Use for system safety as a specialty within systems engineering: the five-step safety risk management process (mandated by FAA Order 8040.4 and the AMS), the severity-by-likelihood risk matrix and acceptance bands, the Safety Order of Precedence, system-description models (5M, SHEL(L)), the hazard-analysis activities (PHL/PHA/RHA/SSHA/SHA/O&SHA/HHA), integrated system hazard analysis, the analysis techniques (FTA, FMEA/FMECA, Fault Hazard, Common Cause, Sneak Circuit, Energy Trace), closed-loop hazard tracking (Safety Action Record), and the specialty domains the handbook covers — acquisition-lifecycle safety and contracting (OSA/CSA/SSPP/ISSPP), software safety (control-capability ranking, SHCM, DO-178B), test & evaluation safety, facilities safety, commercial launch safety (AST licensing, expected casualty, MPL/DAMP), system safety training, and Operational Risk Management (ORM) with human/organizational factors. Scope limits: this is a year-2000 FAA how-to; it predates the modern FAA/ICAO Safety Management System (SMS) and Just Culture regulatory frameworks, and many cited orders, advisory circulars, and launch-licensing rules have since been restructured. It is FAA/NAS-centric, leans on MIL-STD-882C, and does not define the SE life-cycle processes themselves (use nasa-npr-7123 / dau-se-guidebook) or reproduce third-party standard text (MIL-STD-882, DO-178B, ISO/IEC/IEEE).

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Orquestación multimodelo y estrategia de cambio de modelos. Selección de modelos basada en puntuación, delegación entre agentes (Gemini, Codex, Ollama), emparejamiento con advisor, disparadores de escalado, matriz de permisos y optimización de eficiencia de costos.

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Extrem kurzes, substantielles Ausgabe-FORMAT fuer EINE Entscheidung oder Entscheidungsgruppe, wenn die dahinterstehende Analyse bereits existiert (im Chat entstanden, aus `decide`, aus `decision-briefing`, aus einem Ticket oder Dokument): Kontext in 2-3 Zeilen, Pro/Contra je Option als Stichpunkte im paveman/caveman-reduzierten Stil, Talking Points, eine begruendete Empfehlung, und ein Pfad/Link zur Vollanalyse zum Komplett-Nachlesen. Nutze diesen Skill bei "gib mir das kurz mit Pro Contra und Empfehlung", "executive summary zur Entscheidung", "kurzkontext zu <Thema>", "fass die Entscheidung X in einer Kurzform zusammen", /decision-shot, oder wenn ein Ergebnis an jemanden weitergereicht werden soll, der nur die Essenz braucht, nicht die volle Herleitung. NICHT nutzen, um viele offene Entscheidungen zu SAMMELN und per Buchstabe abzufragen -- das macht decision-briefing; NICHT nutzen, um eine Entscheidung erst zu ANALYSIEREN -- das macht decide. decision-shot setzt eine fertige Analyse voraus und komprimiert nur deren Praesentation.

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Use when the user asks to "build our social crisis protocol", "mentions are exploding — what do we do first", or "when do we pause the posting queue"; produces a 1-5 severity ladder with tunable Estimated trigger thresholds (mention-velocity multiples vs the 7-day listening baseline, sentiment flip, journalist/regulator contact, employee-conduct class), the first-mechanical-action rule — pause ALL scheduled posts AND paid amplification, with dated state markers dropped to the channels proposal protocol and reconciled post-incident — a pre-approved holding-statement library with committed update cadences, when-NOT-to-post rules, a spokesperson/approval matrix, and a post-crisis retro template; re-runs the social-quality-auditor pre-publish gate before un-pausing the queue. Not for email deliverability incidents (blocklist, spam-rate spikes) — use deliverability-qa; inside a launch window launch-day-conductor owns incident handling. 社媒危机预案/暂停队列/声明库/发言人矩阵

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検査器（linter / ガード / CI ゲート / バリデータ / フィルタ）そのものの検出力が差分で静かに落ちていないか、また新設した検査が実際に検出できることが実証されているかを diff-time で検出する。Check 1 unverified detection reduction（検査器の検出ロジック（正規表現・除外パターン・解決集合・allowlist・baseline）を変更して検出件数が減ったのに、減った 1 件ずつが誤検出であった根拠が示されないまま「誤検出を潰した」「N → M 件に減少」と改善として主張している）、Check 2 unproven guard detection power（新しい検査ロジック・CI ゲート・バリデーションを追加・変更したのに、検出すべき入力を注入して検出されること／検出すべきでない入力で黙ることを示す変異注入の証跡が無く、「追加した」「CI が緑」を検出力の証拠として扱っている）、の 2 Check を対象とする report-only。テスト自身のアサーションが実質何も検証していない構造は test-assertion-effectiveness、レビュー基準・品質ゲートの明示的な弱体化（ルールの削除・閾値の引き下げ・suppression entry 追加・lint ルールの無効化）は review-criteria-integrity、リファクタ完了主張一般の検証は refactor-claim-audit、workflow の permissions と action pin は gha-workflow-security、設定ファイルの構文・型の妥当性は config-json へ委譲する

Determine whether to expense or depreciate business assets using the de minimis safe harbor ($2,500), Section 179 ($1,220,000 limit), bonus depreciation (phase-out 2024–2027), or MACRS schedules. Track asset basis, useful life, and accumulated depreciation. Trigger on "depreciate", "Section 179", "bonus depreciation", "capitalize or expense", "asset tracking", "de minimis".

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Civic tech v2 — transparency, open data, citizen participation, e-government

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ALWAYS use this skill whenever you would otherwise ask the user a question in free-form chat -- for clarifications, confirmations (especially destructive actions), missing parameters, multiple-choice decisions, or structured form input. Elicitations are routed through the Unique AI Platform UI via `unique-cli elicit create` + `elicit wait` (the default polling pattern) or a single supervised `unique-cli elicit ask` call (only when your agent instructions say your environment supervises the wait) so the user gets a proper structured prompt and you get a structured answer back. Do NOT ask the user in plain chat when you can use this skill instead.

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Designs and documents structured incident response playbooks that define step-by-step procedures for specific incident types aligned with NIST SP 800-61r3 and SANS PICERL frameworks. Covers playbook structure, decision trees, escalation criteria, RACI matrices, and integration with SOAR platforms. Activates for requests involving IR playbook creation, incident response procedure documentation, response runbook development, or SOAR playbook design.

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End-to-end alternative splicing analysis from FASTQ to differential splicing results for short-read bulk RNA-seq. Aligns with STAR 2-pass cohort-style, performs junction QC (RSeQC, MaxEntScan, SpliceAI), runs rMATS-turbo and leafcutter for concordant differential analysis, optionally MAJIQ V3 for complex events / heterogeneous cohorts, isoform-switching with NMD/ORF/domain consequences (IsoformSwitchAnalyzeR v2 + DRIMSeq+DEXSeq+stageR DTU), and sashimi visualizations. Use when performing comprehensive splicing analysis from raw bulk RNA-seq data; for variant-driven splice prediction see splice-variant-prediction; for rare-disease single-patient outlier detection see outlier-splicing-detection; for full-isoform PacBio/ONT analysis see long-read-splicing.

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Drug and chemical toxicity assessment via adverse outcome pathways (AOPs), real-world FAERS adverse event signals, FDA labels, and toxicogenomic associations. Triangulates molecular initiating event to cellular outcome to organ-level toxicity to clinical adverse event. Use for hepatotoxicity/cardiotoxicity/nephrotoxicity prediction and toxicology reports.

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Deploys infrastructure on Oracle Cloud Infrastructure (OCI) with ARM64 instances (Always Free tier eligible). Handles compartments, VCNs, subnets, security lists, and compute instances. Use when: setting up Oracle Cloud infrastructure, deploying ARM64 instances, troubleshooting OUT_OF_HOST_CAPACITY errors, optimizing for Always Free tier. Keywords: oracle cloud, OCI, ARM64, VM.Standard.A1.Flex, Always Free tier, OUT_OF_HOST_CAPACITY, oci compartment, oci vcn

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pen.dev CLI（`pencil` / `pen`コマンド）だけを使って、.penファイル（pen.devで作成されたデザインファイル）の中のNodeのデザインデータ（属性・構造）とスクリーンショット画像を読み取り専用で取得するスキル。Node ID指定に加え、名前の正規表現（例: 「ヘッダー」「.*Button」）、Nodeタイプ（frame / text / image など）、再利用可能コンポーネント、特定Node配下、ドキュメント全体のトップレベルなど、**ID以外の指定方法**にも対応する。ユーザーが「.penのこのNodeの中身を見せて」「特定コンポーネントのデザインデータを取り出して」「Nodeのスクリーンショットだけ欲しい」「ヘッダーの構造を確認したい」「ボタンのスタイルをコピーしたい」「再利用可能コンポーネント一覧を見せて」「ドキュメント全体の構造を覗きたい」「全てのテキストNodeを取得して」のように.pen内の要素の調査・参照・確認・抜き出しを依頼した場合に必ずこのスキルを使う。インタラクティブモード（`pencil interactive`）で `execute` の `Get` / `Print`（読み取り専用関数のみ）を使ってNode属性を取得し、同じ `execute` の `Export` / `TakeScreenshot` で画像を`.pen`と同階層の`snapshots/`にPNG出力する。編集はしない（`save()` も変更系関数も呼ばない）ため、対象ファイルは絶対に書き換わらない。pen.dev MCPには依存せず`pencil`コマンドのみで完結。

Balances Hi-C contact matrices (ICE via cooler.balance_cooler, KR/SCALE/VC context), computes distance-decay expected with cooltools (expected_cis per-diagonal P(s), expected_trans scalar), builds observed/expected (O/E) matrices, and diagnoses polymer state from the P(s) log-derivative. Covers the within-matrix-vs-cross-sample distinction (balancing is NOT a normalizer), the equal-visibility assumption that CNV/aneuploidy violates (use raw counts for copy-number), cis-only balancing, mad_max/blacklist masking before balancing, multiplicative cooler weights vs divisive juicer weights, and the resolution-vs-depth budget. Use when balancing a .cool/.mcool, computing expected or P(s), making O/E matrices for compartments/loops, deciding ICE vs KR vs SCALE, choosing a resolution for a given depth, or troubleshooting NaN/all-NaN balanced matrices; route cross-sample comparison to hic-differential.

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Analyzes species-environment relationships with constrained ordination (CCA, RDA, db-RDA), variance partitioning, indicator species (indicspecies IndVal.g group-equalized), PERMANOVA paired MANDATORILY with PERMDISP (Anderson & Walsh 2013; dispersion confounds centroid tests), Joint Species Distribution Models (HMSC, sjSDM, gjam) with explicit rejection of "residual covariance equals biotic interaction", phylogenetic community ecology (SES_MPD/MNTD), trait-environment via RLQ + fourth-corner with corrected modeltype=6 (Dray 2014), bipartite network metrics (NODF, modularity) with curveball null (Strona 2014), and Mantel-test replacements (dbRDA, GDM) for spatial data. Use when testing how environmental gradients structure communities, identifying habitat indicator taxa, partitioning variance among predictors, deciding whether PERMANOVA significance is location vs dispersion, picking among HMSC/sjSDM/gjam, or replacing Mantel tests for landscape data.

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Assigns pathogen lineages (SARS-CoV-2 Pangolin via UShER mode; Nextclade clade + QC; pango-designation alias_key.json resolution) and tracks variant frequencies over time using Nextstrain (Augur + Auspice), wastewater deconvolution (Freyja, COJAC, alcov, lineagespot), lineage fitness modelling (Wenseleers / Bedford-Figgins multinomial logistic), and recombinant detection (3SEQ, RDP4, Bolotie). Covers Pangolin pangolin-data version pinning (mandatory for reproducibility), Nextclade dataset versioning (lineage-defining mutations change with dataset), Freyja barcode forward-only date constraint, ARTIC primer scheme version churn (V3 / V4 / V4.1 / V5.3.2 / Midnight 1200) with documented dropout regions, recombinant X-prefix Pango designation lag, GISAID vs INSDC dual-deposition tensions, and the Karthikeyan 2022 wastewater early-detection signal with explicit reproducibility caveats. Use when assigning Pango lineages and Nextclade clades to viral consensus sequences, building Nextstrain Augur surveillance pipelines, deconvolving wastewater pooled samples into lineage frequencies with Freyja, tracking lineage frequencies and growth advantages over time, pinning pangolin-data / Nextclade dataset versions for reproducibility, handling ARTIC primer dropouts (V4.1 amplicons 64 / 76 / 88-90), or running variant surveillance for SARS-CoV-2 / influenza / Mpox / RSV / H5N1 / measles.

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Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

日本語の概要は準備中です。原文の説明を表示しています。

CI環境で安定動作するPythonテスト（特にstructlogのログ検証）を書く際に使用。「CIで失敗する」「capture_logsが不安定」「ログアサーションがCIだけ落ちる」「caplogフォールバック」「ANSI混入」「xdistでログが取れない」という課題で起動。capture_logs→caplogフォールバック→ANSI正規化→アサーション設計の流れで堅牢なテストを書く。

When the user wants to apply psychological principles, mental models, or behavioral science to marketing. Also use when the user mentions 'psychology,' 'mental models,' 'cognitive bias,' 'persuasion,' 'behavioral science,' 'why people buy,' 'decision-making,' 'consumer behavior,' 'anchoring,' 'social proof,' 'scarcity,' 'loss aversion,' 'framing,' or 'nudge.' Use this whenever someone wants to understand or leverage how people think and make decisions in a marketing context.

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Ground framework-specific implementation in official documentation — detect stack versions, fetch authoritative docs, implement cited patterns, cite sources. Load when building with a library or framework where API correctness matters, when the user wants verified or documented code, or before writing framework code from memory. Also triggers on "source driven development", "cite the docs", "check official documentation", "verify against docs", "don't hallucinate APIs". Not for pure logic or renames. Pairs with feature-spec and test-driven-development.

日本語の概要は準備中です。原文の説明を表示しています。

Balances Hi-C contact matrices (ICE via cooler.balance_cooler, KR/SCALE/VC context), computes distance-decay expected with cooltools (expected_cis per-diagonal P(s), expected_trans scalar), builds observed/expected (O/E) matrices, and diagnoses polymer state from the P(s) log-derivative. Covers the within-matrix-vs-cross-sample distinction (balancing is NOT a normalizer), the equal-visibility assumption that CNV/aneuploidy violates (use raw counts for copy-number), cis-only balancing, mad_max/blacklist masking before balancing, multiplicative cooler weights vs divisive juicer weights, and the resolution-vs-depth budget. Use when balancing a .cool/.mcool, computing expected or P(s), making O/E matrices for compartments/loops, deciding ICE vs KR vs SCALE, choosing a resolution for a given depth, or troubleshooting NaN/all-NaN balanced matrices; route cross-sample comparison to hic-differential.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes species-environment relationships with constrained ordination (CCA, RDA, db-RDA), variance partitioning, indicator species (indicspecies IndVal.g group-equalized), PERMANOVA paired MANDATORILY with PERMDISP (Anderson & Walsh 2013; dispersion confounds centroid tests), Joint Species Distribution Models (HMSC, sjSDM, gjam) with explicit rejection of "residual covariance equals biotic interaction", phylogenetic community ecology (SES_MPD/MNTD), trait-environment via RLQ + fourth-corner with corrected modeltype=6 (Dray 2014), bipartite network metrics (NODF, modularity) with curveball null (Strona 2014), and Mantel-test replacements (dbRDA, GDM) for spatial data. Use when testing how environmental gradients structure communities, identifying habitat indicator taxa, partitioning variance among predictors, deciding whether PERMANOVA significance is location vs dispersion, picking among HMSC/sjSDM/gjam, or replacing Mantel tests for landscape data.

日本語の概要は準備中です。原文の説明を表示しています。

Assigns pathogen lineages (SARS-CoV-2 Pangolin via UShER mode; Nextclade clade + QC; pango-designation alias_key.json resolution) and tracks variant frequencies over time using Nextstrain (Augur + Auspice), wastewater deconvolution (Freyja, COJAC, alcov, lineagespot), lineage fitness modelling (Wenseleers / Bedford-Figgins multinomial logistic), and recombinant detection (3SEQ, RDP4, Bolotie). Covers Pangolin pangolin-data version pinning (mandatory for reproducibility), Nextclade dataset versioning (lineage-defining mutations change with dataset), Freyja barcode forward-only date constraint, ARTIC primer scheme version churn (V3 / V4 / V4.1 / V5.3.2 / Midnight 1200) with documented dropout regions, recombinant X-prefix Pango designation lag, GISAID vs INSDC dual-deposition tensions, and the Karthikeyan 2022 wastewater early-detection signal with explicit reproducibility caveats. Use when assigning Pango lineages and Nextclade clades to viral consensus sequences, building Nextstrain Augur surveillance pipelines, deconvolving wastewater pooled samples into lineage frequencies with Freyja, tracking lineage frequencies and growth advantages over time, pinning pangolin-data / Nextclade dataset versions for reproducibility, handling ARTIC primer dropouts (V4.1 amplicons 64 / 76 / 88-90), or running variant surveillance for SARS-CoV-2 / influenza / Mpox / RSV / H5N1 / measles.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

日本語の概要は準備中です。原文の説明を表示しています。