429 件 ・ 関連度順
概要と使いどころ
Specialized skill for building production-ready serverless applications on AWS. Covers Lambda functions, API Gateway, DynamoDB, SQS/SNS event-driven patterns, SAM/CDK deployment, and cold start optimization.
日本語の概要は準備中です。原文の説明を表示しています。
Integracao completa com Amazon Alexa para criar skills de voz inteligentes, transformar Alexa em assistente com Claude como cerebro (projeto Auri) e integrar com AWS ecosystem (Lambda, DynamoDB, Polly, Transcribe, Lex, Smart Home).
日本語の概要は準備中です。原文の説明を表示しています。
Define, documenta e aplica a voz da marca — formalidade, personalidade, vocabulário preferido, palavras proibidas e tratamento do leitor. Use quando o usuário disser "voz da marca", "tom de voz", "brand voice", "guia de estilo", "padronizar o tom", "não parece nossa marca", "manual de redação", ou quando precisar auditar se um texto está aderente. Extrai a voz de amostras reais em vez de inventar adjetivos, e grava o resultado na seção 5 de .claude/pwdev-copy-context.md para todas as demais skills consumirem.
日本語の概要は準備中です。原文の説明を表示しています。
Organize, categorize, track, and manage invoices systematically with automated extraction of invoice data, payment tracking, and financial organization. Use when processing invoice uploads, extracting invoice details (date, amount, vendor), categorizing expenses, tracking payment status, organizing receipts, generating financial reports, or building accounting and bookkeeping systems.
日本語の概要は準備中です。原文の説明を表示しています。
Integracao completa com Amazon Alexa para criar skills de voz inteligentes, transformar Alexa em assistente com Claude como cerebro (projeto Auri) e integrar com AWS ecosystem (Lambda, DynamoDB, Polly, Transcribe, Lex, Smart Home).
日本語の概要は準備中です。原文の説明を表示しています。
Detects TAD boundaries from balanced Hi-C contact matrices via the diamond-window insulation score (cooltools insulation) and HiCExplorer hicFindTADs, returning a continuous log2 insulation track, valley-prominence boundary_strength, and Li/Otsu-thresholded is_boundary flags across a list of window sizes. Covers the multi-scale window sweep (sub-TAD to compartment-domain), why the boundary is reproducible but the domain partition is not, cross-condition comparison via differential SCORE not differential partition, and the insulation-vs-compartment orthogonality. Use when calling TADs or domain boundaries, computing insulation scores, choosing a window size, ranking boundary strength, comparing boundaries across conditions, or annotating CTCF-backed boundaries; route domain rendering to hic-visualization and boundary-feature overlap to genome-intervals.
日本語の概要は準備中です。原文の説明を表示しています。
Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.
日本語の概要は準備中です。原文の説明を表示しています。
Simulate transcription factor perturbation effects on cell state in silico with CellOracle and Dynamo, and predict transcriptional responses to genetic perturbations with GEARS, scGen, and CPA. Covers the direction-not-magnitude principle, local-linear validity, the GRN/velocity error it inherits, baseline discipline (mean and additive baselines), and the validation gap. Use when predicting TF knockout or overexpression effects, ranking driver TFs for fate transitions, or planning perturbation experiments. For GRN construction see multiomics-grn; for experimental Perturb-seq see single-cell/perturb-seq.
日本語の概要は準備中です。原文の説明を表示しています。
Tests genotype-environment associations and identifies adaptive loci while correcting for the four-confound landscape (structure, demography, background selection, sampling design) using LFMM2 with mandatory K via sNMF cross-entropy elbow (LEA 3), BayPass Core/AUX/C2/IS with Omega covariance matrix, RDA / pRDA for polygenic adaptation (Forester 2018; requires imputed genotypes), OutFLANK with trimmed FST null, pcadapt, gradient forests (Ellis-Smith-Pitcher 2012, NOT mis-cited Ellis-Manel), Capblancq & Forester 2021 RDA Swiss-army-knife, genomic-offset prediction with Lind & Lotterhos 2025 three-regime caveat, Lotterhos-Whitlock sampling optima, Wang & Bradburd 2014 IBD vs IBE, and Circuitscape + ResistanceGA. Use when identifying adaptive loci across gradients, choosing K for LFMM2, deciding among GEA methods, predicting maladaptation with the novel-environment caveat, distinguishing IBD vs IBE, or optimizing sampling design.
日本語の概要は準備中です。原文の説明を表示しています。
Delimits putative species boundaries from molecular data within the de Queiroz 2007 unified-lineage framework using ASAP (Puillandre 2021 successor to ABGD), mPTP C++ (Kapli 2017 successor to bPTP; bPTP is Python NOT R), GMYC single/multi-threshold (Pons 2006; Fujisawa 2013), multilocus BPP v4 with prior calibration from data (NOT defaults; Yang 2015), SNAPP + BFD* for SNP delimitation, DELINEATE (Sukumaran 2021) speciation-process modeling to address Sukumaran & Knowles 2017 PNAS critique that MSC delimits structure not species, integrative-taxonomy congruence (Padial 2010; Carstens 2013), Dsuite for introgression testing before sister claims (Malinsky 2021), and Meyer & Paulay 2005 barcoding-gap-absence caveat. Use when delineating species from DNA barcoding data, resolving cryptic complexes, choosing among ASAP/mPTP/BPP/DELINEATE, calibrating BPP priors, distinguishing introgression from ILS, or applying the Sukumaran-Knowles oversplitting correction.
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Cross-method decision tree for calling hits in pooled CRISPR screens. Catalogs statistical models (MAGeCK RRA, MAGeCK MLE, BAGEL2, drugZ, JACKS, Chronos, CERES), experimental designs each is built for, failure modes outside design domain, reconciliation when methods disagree, multiple-testing and effect-size thresholds, the order of operations (count -> QC -> CN-correct -> hit-call -> validate), the second-best-sgRNA conservative rule, and consensus-hit strategy. Use when choosing among MAGeCK / BAGEL2 / drugZ / JACKS / Chronos for a given design, reconciling disagreement across two or three methods on the same screen, deciding whether to require consensus, gating downstream validation by hit-confidence tier, or interpreting unstable hit lists across reruns.
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Build forest plots (HR, OR, RR, beta-coefficient summaries with CIs) and funnel plots (meta-analysis publication-bias diagnostics) using forestplot, metafor, ggforest, and MendelianRandomization with proper axis-scaling, summary-diamond placement, subgroup nesting, and Egger / trim-and-fill asymmetry tests. Use when summarizing effects across subgroups, trials, or instruments — meta-analysis, Mendelian randomization, subgroup HRs.
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Infer integer allele-specific copy number, tumor purity, and ploidy from tumor sequencing by jointly modeling read depth (logR) and B-allele frequency (BAF) with ASCAT, Sequenza, FACETS, PURPLE, and PureCN (tumor-only). Covers the purity-ploidy identifiability problem, the diploid-baseline (dipLogR) anchor, major/minor copy number, loss of heterozygosity, sunrise/contour fit diagnostics, and reconciliation of conflicting fits. Use when tumor analysis needs absolute copy number rather than relative log2, when estimating purity and ploidy, calling LOH or copy-neutral LOH, resolving whole-genome doubling, running tumor-only allele-specific calling, or choosing among ASCAT, Sequenza, FACETS, and PureCN.
日本語の概要は準備中です。原文の説明を表示しています。
Detect and adjust for horizontal pleiotropy in two-sample Mendelian randomization by distinguishing uncorrelated (UHP) from correlated (CHP) pleiotropy and choosing among Egger, MR-PRESSO, MR-RAPS, CAUSE, LHC-MR, LCV, MR-Clust, MR-Mix, and contamination-mixture methods. Use when validating an MR causal claim, running the STROBE-MR sensitivity battery, suspecting a shared heritable confounder, working under weak-instrument or polygenic-exposure regimes, or reconciling discordant estimates across robust methods.
日本語の概要は準備中です。原文の説明を表示しています。
Generate A2UI 0.8 protocol compliant UI code. Use when building agent-driven interfaces, generating JSONL messages for surfaceUpdate/dataModelUpdate/beginRendering, creating forms, lists, cards, or any UI components that render across web/mobile/desktop platforms.
日本語の概要は準備中です。原文の説明を表示しています。
Use when you need to review, improve, or refactor Java code for generics quality — including avoiding raw types, applying the PECS (Producer Extends Consumer Super) principle for wildcards, using bounded type parameters, designing effective generic methods, leveraging the diamond operator, understanding type erasure implications, handling generic inheritance correctly, preventing heap pollution with @SafeVarargs, and integrating generics with modern Java features like Records, sealed types, and pattern matching. Part of the skills-for-java project
日本語の概要は準備中です。原文の説明を表示しています。
パフォーマンス改善作業の開発ドキュメントを自動生成します。YYYYMMDD_要件名の形式でディレクトリを作成し、requirements.md、design.md、tasklist.md、testing.mdを生成します。「パフォーマンス改善のドキュメント作って」「最適化作業のドキュメント」「高速化のドキュメント生成」などで呼び出されます。
開発作業のタスクリスト（tasklist.md）を生成します。このスキルは単独で使用することも、generate-working-docsから呼び出されることもあります。
開発作業の設計書（design.md）を生成します。このスキルは単独で使用することも、generate-working-docsから呼び出されることもあります。
開発作業の要件定義書（requirements.md）を生成します。このスキルは単独で使用することも、generate-working-docsから呼び出されることもあります。
開発作業のテスト手順書（testing.md）を生成します。このスキルは単独で使用することも、generate-working-docsから呼び出されることもあります。
新規機能開発の作業ドキュメントを自動生成します。YYYYMMDD_要件名の形式でディレクトリを作成し、requirements.md、design.md、tasklist.md、testing.mdを生成します。「開発作業ドキュメント作成」「新規開発のドキュメント作って」「ドキュメント生成」などで呼び出されます。パフォーマンス改善作業には generate-performance-docs を使用してください。
開発作業の設計書（design.md）を単体で生成・再生成します。全ドキュメントの一括生成には generate-working-docs（新規機能開発）または generate-performance-docs（パフォーマンス改善）を使用してください。
開発作業のテスト手順書（testing.md）を単体で生成・再生成します。全ドキュメントの一括生成には generate-working-docs（新規機能開発）または generate-performance-docs（パフォーマンス改善）を使用してください。