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Annotate bacterial and archaeal genomes and plasmids with Bakta's Prodigal/HMM/diamond pipeline. Identifies CDS, ncRNA, tRNA, rRNA, tmRNA, sORFs, CRISPR arrays, oriC/oriV/oriT, and gaps against a curated UniRef-derived database. Produces NCBI-compatible GFF3, GenBank, EMBL, JSON, FASTA, TSV, and a circular genome plot. Use Prokka for legacy pipelines or non-bacterial kingdoms; PGAP for NCBI GenBank submission.

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Look up EMDB cryo-EM density maps and fitted atomic models via the entry REST API + EBI Search WS. Fetch entry metadata (resolution, method, organism, sample), map download URLs, fitted PDB IDs, and citations. Keyword search via EBI Search. No auth. For atomic coordinates use pdb-database; for AlphaFold predictions use alphafold-database-access.

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Query UniProt REST API: search by gene/protein name, fetch FASTA, map IDs (Ensembl, PDB, RefSeq), access Swiss-Prot annotations. Use bioservices for multi-DB access; alphafold-database-access for structures.

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Python library for single-particle tracking (SPT) in video microscopy via the Crocker-Grier algorithm. Locate particles (fluorescent spots, colloids, vesicles, cells) per frame, link into trajectories, filter short tracks, and compute MSD for diffusion analysis. 2D/3D with subpixel accuracy; reads TIF stacks, AVI, image series via pims. Use for quantitative SPT and diffusion coefficient extraction from fluorescence or brightfield video.

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Auto-annotate plasmids with features (promoters, terminators, resistance, origins, tags, fluorescent proteins) via BLAST against curated DBs (Addgene, fpbase, SnapGene). FASTA or raw sequence in; annotated GenBank, interactive HTML maps, CSV tables out. Handles circular topology. Use to verify synthetic constructs, prep Addgene submissions, share maps, or batch-annotate cloning libraries.

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Statistical visualization on matplotlib with native pandas support. Auto aggregation, CIs, grouping for distributions (histplot, kdeplot), categorical (boxplot, violinplot), relational (scatterplot, lineplot), regression (regplot, lmplot), matrix (heatmap, clustermap), grids (pairplot, FacetGrid). Use for quick statistical summaries; matplotlib for fine control; plotly for interactive HTML.

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Time-to-event modeling with scikit-survival: Cox PH (elastic net), Random Survival Forests, Boosting, SVMs for censored data. C-index, Brier, time-dependent AUC; Kaplan-Meier, Nelson-Aalen, competing risks. Pipeline/GridSearchCV compatible. Use statsmodels for frequentist, pymc for Bayesian, lifelines for parametric.

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Maintain the canonical TASKS.csv with permanent IDs, provenance and lifecycle history; list, add, update, complete, cancel or import selected actions.

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Get option chain data including calls and puts with strikes, bids, asks, volume, open interest, and implied volatility. Use when user asks about options, option prices, calls, puts, or option chain for a specific expiration date.

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Get option chain data from Interactive Brokers for equities, ETFs, and futures (FOP), including calls and puts with strikes, bids, asks, volume, implied volatility, and model Greeks. Use when user asks about options using IBKR data, futures options (NQ/ES/CL/GC...), or needs real-time option quotes from their broker. Requires TWS or IB Gateway running locally.

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Build automated BIM validation pipelines for IFC/Revit data. Continuous validation against IDS, LOD requirements, COBie, and project-specific BEP standards.

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Compare and analyze contractor bids. Score proposals, identify scope gaps, and recommend selections.

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Analyze contractor bids against CWICR benchmarks. Identify pricing anomalies, compare bid components, and support bid evaluation decisions.

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Analyze and compare subcontractor bids against CWICR benchmarks. Evaluate pricing, identify outliers, and support negotiation.

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Tendering and reporting in OpenConstructionERP: prepare tender BOQs, compare bids, manage risks, and generate reports (weekly, monthly, closeout). Use when a project goes to tender or needs reporting.

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Builds estimates, quotes, and bids for trades and field-service businesses (HVAC, plumbing, electrical, roofing, landscaping, painting, cleaning, remodeling, general contracting, IT/AV installs). Turns a scope into line items with waste factors, burdened labor, materials, equipment, subs, permits, mobilization, contingency, overhead, profit, and tax, then writes a client-ready xlsx (live formulas) and PDF plus an internal cost sheet, with good/better/best options, add-ons, a payment schedule, exclusions, and assumptions. Use whenever someone asks for a quote, estimate, bid, proposal price, or job price, asks "how much should I charge for this job", wants to check a price or margin on a job, or is confused about markup vs margin. Computes deterministically with a script instead of mental math, because models routinely treat a 30% markup as a 30% margin and drop labor burden, waste, and mobilization.

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Runs small and midsize business bookkeeping operations - categorizes bank and card transactions to a chart of accounts, reconciles a bank or card statement against the ledger, works a month-end close checklist, and produces a close package for the accountant. Works from QuickBooks Online, Xero, bank CSV/OFX, or Mercury exports, and from the Xero, QuickBooks, or Mercury connectors when present. Use it whenever the user says "reconcile", "bank rec", "categorize transactions", "month end close", "close the books", "books don't match the bank", "reconciliation is off by", "uncategorized expenses", or shares a QuickBooks or Xero export or a bank statement CSV, even if they do not name the task. Never plugs a difference; every unreconciled dollar is explained or listed.

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Best practices and example-driven guidance for building SwiftUI views and components, including navigation hierarchies, custom view modifiers, and responsive layouts with stacks and grids. Use when creating or refactoring SwiftUI UI, designing tab architecture with TabView, composing screens with VStack/HStack, managing @State or @Binding, building declarative iOS interfaces, or needing component-specific patterns and examples.

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Create distinctive, production-grade frontend interfaces with high design quality. Use this skill when the user asks to build web components, pages, or applications. Generates creative, polished code that avoids generic AI aesthetics.

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Use davidsandberg/facenet for TensorFlow 1.x face recognition workflows: MTCNN alignment, embeddings, classification, LFW evaluation, training, and model export.

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Shared discipline for every hunt-* skill: scope and authorization gating, the two-account rule, the confirmation gate that separates a real finding from a false positive, enumeration limits, stop conditions, marker discipline, wiki-first query and self-heal, FIND output, Deadends, and wiki distillation. ALWAYS LOADED alongside any hunt skill. Also trigger directly on "is this in scope", "is this a real bug", "how do I confirm this", "should I keep going", "how many IDs should I test", "what severity", "how do I report this", "I got someone else's data". Every hunt-* skill assumes this file; without it they run without their safety and quality layer.

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Create distinctive, production-grade frontend interfaces with high design quality. Use this skill when the user asks to build web components, pages, or applications. Generates creative, polished code that avoids generic AI aesthetics.

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Use when generating or refactoring Spring Boot 4 JPA entities, repositories, queries, projections, persistence tests, entity relationships, embeddables, IDs, or Hibernate mappings. Covers Jakarta Persistence 3.2 imports, Hibernate 7 entity modeling, new-state detection, N+1 prevention, projections, keyset pagination, batch writes, and common agent mistakes.

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Create, edit, validate, and run Agent QA tests, suites, and hooks through MCP or CLI while preserving canonical IDs and schema contracts.

日本語の概要は準備中です。原文の説明を表示しています。