Workflow for read alignment, sorting, indexing, mapping statistics, and downstream-ready alignment artifacts.
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概要と使いどころ
Workflow for read alignment, sorting, indexing, mapping statistics, and downstream-ready alignment artifacts.
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Cell-free protein synthesis (CFPS) planning and optimization guidance. Use when: (1) Planning CFPS experiments, (2) Troubleshooting low yield or aggregation, (3) Optimizing DNA template design for CFPS, (4) Expressing difficult proteins (disulfide-rich, toxic, membrane).
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Workflow for orthology, synteny, ancestral reconstruction, and evolutionary comparison across genomes.
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Workflow for RNA modification analysis such as m6A peak calling, differential modification, and transcript-level visualization.
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The craft of designing icons that communicate instantly across cultures, contexts, and scales. Icon design bridges semiotics, cognitive psychology, and visual craft to create symbols that users understand without thinking. Great icons are invisible in the best way - they convey meaning so naturally that users never pause to decode them. This skill covers icon grid systems, optical alignment, stroke consistency, metaphor selection, scalability across sizes, SVG optimization, and icon set coherence. The best icon designers understand that icons are a visual language - each icon must speak the same dialect while carrying its own distinct meaning. Use when "icon, iconography, symbol, glyph, icon set, icon library, pictogram, svg icon, icon grid, icon pack, feather icons, lucide, phosphor, heroicons, icon system, icon style, icons, iconography, svg, symbols, glyphs, pictograms, ui-icons, icon-set, visual-design, design-system" mentioned.
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AMAZON ALEXA — Voz Inteligente com Claude workflow skill. Use this skill when the user needs Integracao completa com Amazon Alexa para criar skills de voz inteligentes, transformar Alexa em assistente com Claude como cerebro (projeto Auri) e integrar com AWS ecosystem (Lambda, DynamoDB, Polly, Transcribe, Lex, Smart Home) and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.
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AMAZON ALEXA — Voz Inteligente com Claude workflow skill. Use this skill when the user needs Integracao completa com Amazon Alexa para criar skills de voz inteligentes, transformar Alexa em assistente com Claude como cerebro (projeto Auri) e integrar com AWS ecosystem (Lambda, DynamoDB, Polly, Transcribe, Lex, Smart Home) and the operator should preserve the upstream workflow, copied support files, and provenance before merging or handing off.
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健身私教 — 为客户设计、指导和推进个性化训练方案的专业工艺, 有别于团课教练、物理治疗师或运动队教练: (a) 训练方案设计与周期化 (线性 / 波动 / 板块周期化 Bompa & Haff; NSCA《力量训练与体能训练精要》行业圣经; Schoenfeld 肌肥大机制; Helms/Valdez/Morgan 肌肉与力量金字塔; 共轭训练法 Simmons/Westside; Starting Strength 线性渐进 Rippetoe; Renaissance Periodization 训练量地标 Israetel); (b) 动作评估与筛查 (FMS 功能性动作筛查 Gray Cook; NASM CEx 纠正性训练连续体 抑制-拉伸-激活-整合; 关节逐级方法 Cook/Boyle; 姿势与代偿模式识别; PAR-Q+ 与 ACSM 运动前筛查指南); (c) 教学与指令 (外部 vs 内部注意焦点 Wulf; 差异化学习 Schöllhorn; 约束导向方法 Davids/Renshaw; RIR/RPE 自动调节 Zourdos/Helms; 动机访谈在健身中的应用 Rollnick/Miller); (d) 营养指导的执业边界 (宏量营养素周期化; Precision Nutrition / ISSN 立场声明的循证建议; 边界: 私教提供一般性指导, 医学营养治疗需 RD/RDN 资质; 能量可用性与 RED-S Mountjoy IOC 共识); (e) 商业与客户管理 (客户留存与方案依从; 咨询与销售伦理; 线上教练平台与规模化 Goodman PTDC; 方案交付工具 TrueCoach/Trainerize/TrainHeroic; 课时定价模型; 独立教练 vs 健身房雇员); (f) 专项方向 (运动表现 CSCS; 纠正性训练 CES; 老年健身 SFN; 产前产后; 青少年 YSCA; 适应性 / 无障碍健身; 健美 / 形体; 力量举; 耐力运动 S&C)。诚实处理: 兄弟科学 vs 循证之争, 补剂行业利益冲突, 合成代谢激素使用现状 (诚实但不推广: 匿名调查约 30% 有经验男性健身者使用过 — 业内估, Kanayama/Pope/Hudson 记录健康风险, WADA 禁用清单用于竞技语境), 体型焦虑与进食障碍风险 (男性私教肌肉变形症, 正食症倾向, NEDA 负责任语言指南), 健身房掠夺性销售 (高压推销、误导性对比照、长约锁定), 执业边界违规 (私教诊断伤病或超范围开饮食处方), 线上教练认证造假 (周末速成、未经认可的认证)。不含: 物理治疗 / 康复治疗 (持照医疗职业), 不含: 团课教练 (Les Mills/Zumba/CrossFit 分馆教练 — 相邻但不同), 不含: 精英竞技运动教练 (团队项目周期化, 不同职业路径), 不含: 营养作为主业 (临床营养学需单独资质)。 (Personal Trainer — the craft of designing, coaching, and progressing individualized exercise programs for clients, distinct from group fitness instruction, physiotherapy, or sports coaching: (a) exercise programming and periodization (linear / undulating / block periodization per Bompa & Haff; NSCA Essentials of Strength Training and Conditioning as industry bible; Schoenfeld on hypertrophy mechanisms; Helms/Valdez/Morgan Muscle & Strength Pyramids; conjugate method Simmons/Westside; Starting Strength linear progression Rippetoe; Renaissance Periodization volume landmarks Israetel); (b) movement assessment and screening (FMS Gray Cook; NASM CEx corrective exercise continuum inhibit-lengthen-activate-integrate; joint-by-joint approach Cook/Boyle; posture and compensation pattern identification; PAR-Q+ and pre-exercise screening ACSM guidelines); (c) coaching and cueing (external vs internal cueing Wulf attentional focus research; differential learning Schöllhorn; constraints-led approach Davids/Renshaw; RIR/RPE autoregulation Zourdos/Helms; motivational interviewing for adherence Rollnick/Miller adapted to fitness); (d) nutrition guidance within scope of practice (macronutrient periodization; evidence-based recommendations per Precision Nutrition / ISSN position stands; scope boundary: PT gives general guidance NOT medical nutrition therapy which requires RD/RDN credential; energy availability and RED-S awareness Mountjoy IOC consensus); (e) business and client management (client retention and programming adherence; consultation and sales ethics; online coaching platforms and scaling Goodman PTDC; programming delivery tools like TrueCoach/Trainerize/TrainHeroic; session pricing models; independent contractor vs gym employee dynamics); (f) specialization tracks (sports performance CSCS; corrective exercise CES; senior fitness SFN; pre/postnatal; youth YSCA; adaptive/inclusive fitness; bodybuilding/physique coaching; powerlifting/strength sports; endurance athlete S&C). Evidence-based practice as the core ethos: Schoenfeld/Krieger meta-analyses on volume-response, Helms systematic reviews on protein/resistance training, Brad Schoenfeld's MASS journal bridging research to practice, Menno Henselmans and Eric Helms as practitioner-researchers, Greg Nuckols Stronger By Science as translation layer. Chinese fitness industry context: rapid growth of personal training in commercial gyms (Keep/Lefit/Megafit chains), NSCA-CPT and ACE-CPT as dominant certifications alongside local CSFA (China Sport and Fitness Association) credential, '体适能' (physical fitness) as formal term, online coaching boom post-COVID, PT business models in tier 1-3 cities diverge sharply. Honest treatment of: bro science vs evidence-based d
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Applies the reasoning of Zhong Lin Wang (nanotechnology pioneer, Georgia Tech) to problems involving energy harvesting, IoT power scaling, sensor networks, and fundamental physics applications. Reach for this skill whenever the user is discussing self-powered systems, scaling distributed hardware, overcoming battery bottlenecks, or translating fundamental scientific phenomena (like static electricity or mechanical strain) into novel engineering applications. It is highly relevant for hardware roadmapping, optoelectronics, piezotronics, and challenging established scientific assumptions (like classical Maxwell's equations) to model dynamic systems.
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Aurora Smart Home orchestrator — routing layer for all smart home skills. Use this skill when the user asks ANY smart home question and you need to decide which skill to invoke, or when a task spans multiple skills (e.g., "build a sensor that shows on a dashboard and triggers automations"). Invoke aurora FIRST before reaching for a specific skill — it will route to the right specialist(s) and recommend the correct Claude model to keep token usage efficient. Trigger on: smart home, Home Assistant, ESPHome, automation, IoT, dashboard, ESP32, Node-RED, or any request about controlling or monitoring devices at home.
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ESPHome device configuration, firmware, and IoT product development. Covers ESP32, ESP32-S3, ESP32-C3, ESP32-C6, ESP32-H2, ESP32-P4, ESP8266, RP2040, RP2350, nRF52, LibreTiny, Shelly, Sonoff, Tuya, BLE proxy, Matter firmware, Thread, Zigbee, GPIO, sensor YAML, LVGL displays, LED strips, voice assistant hardware, device flashing, Arduino conversion, alarm_control_panel, lock, valve, media_player, microphone, speaker, audio DAC, event entities, datetime entities, Z-Wave proxy, MIPI DSI displays, and DLMS smart meters. Also covers designing new ESPHome-based products: hardware selection, component sourcing, PCB design (KiCad), enclosures, 3D printing, CE/FCC certification, BOM optimization, and manufacturing from prototype to production scale.
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Operate the Ryuumonbuchi MCP server for headless Ghidra reverse engineering. Use when driving decompilation, disassembly, patching, type and symbol recovery, memory edits, transactions, and Ghidra project analysis through a Ryuumonbuchi server (stdio or streamable HTTP). Covers the safe read-only-first workflow, session and address discipline, mutation and transaction rules, headless.run, task and error recovery, raw ghidra.call/ghidra.eval/ghidra.script escape hatches, and a sleep/timing-gate bypass recipe.
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OpenAPI 3.1 spec authoring, Swagger UI, automatic client/server code generation (openapi-typescript, Orval, Kiota), contract testing / Penulisan spesifikasi OpenAPI 3.1, Swagger UI, pembuatan kode klien/server otomatis, dan pengujian kontrak.
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IP networking fundamentals for Zephyr RTOS. Covers IoT protocol selection (LwM2M, CoAP, MQTT), IP stack configuration and trimming (IPv4/IPv6, UDP/TCP), and professional SDK integration as Zephyr modules using West manifests. Trigger when building cloud-connected applications, optimizing network memory usage, or integrating external cloud SDKs.
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Go-based security techniques from "Black Hat Go" extended with macOS, Cloud, Mobile, IoT, Supply Chain, API, Web3, AI/ML, Red Team, ATT&CK, and LLM chapters. 186 techniques, 36 tools, 33 defenses across 37 chapters. Includes adversarial bisimulation games with Ungar (order-dependent) and join-semilattice structures. AAIF-compatible multiplayer agent games for human-agent security exercises.
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Author, compile, inspect, and revise WAM low-poly 3D characters, creatures, props, rigs, and animations with ChatGPT Codex. Use for WAM or .wam requests, WoW-style or game-ready models, and one or more reference images or requested render views.
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Author low-poly WoW-style 3D characters — mesh, skeleton, and animations — in the WAM text language, compiled to glTF plus multi-view PNG renders. Use when the user wants to create or edit a stylized/low-poly 3D character, creature, or prop with a rig and animations, supplies one or more reference images or views, mentions WAM or .wam files, or asks for "WoW-style" / game-ready models.
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Deploy machine learning models to edge devices using Google AI Edge Gallery, TensorFlow Lite, ONNX Runtime, and MediaPipe. Covers model quantization (INT8/INT4), on-device inference with Gemma 4 models, Android/iOS deployment via AI Edge Gallery, hardware delegate selection (GPU/NPU/DSP), and performance benchmarking on constrained devices. Use when deploying models to mobile phones, IoT devices, or embedded systems where cloud inference is impractical due to latency, cost, or connectivity constraints.
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Use when you need to classify an IP as internet scanner noise vs. targeted activity. Returns noise/riot flags, classification (benign/malicious/unknown), actor name if known. IP-only. Commonly invoked by /ip-investigation to filter out mass-scanning noise. Retrieval only.
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"Apply Actor-Network Theory (Latour, Callon) to trace how human and non-human actors (actants) form networks through translation processes. Use this skill when the user needs to map sociotechnical assemblages, analyze how innovations stabilize or fail through network-building, trace the four moments of translation (problematization, interessement, enrollment, mobilization), or when they ask 'how did this technology become accepted', 'who and what holds this network together', or 'why did this innovation fail to gain traction'.".
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Maakt regelrecht-scenario's vindbaar en hun norm-ketens leesbaar/geassert. Aan de invoerkant — vertaal een platte lijst leaf-parameters naar benoemde casus-assen + een herbruikbare persona-bibliotheek, met een reverse-lookup casus-matrix. Aan de uitvoerkant — maak de norm-keten zichtbaar via keten-checkpoints (assert elke knoop op het kritieke pad, niet alleen de endpoint) en golden-trace-snapshots + branch-coverage. Gebruik dit bij het schrijven of opschonen van scenario-/feature-files, het terugvinden of ontwerpen van een specifieke casus in een grote variabelenlijst, het reviewen van engine-traces, of het regressie-bestendig maken van cross-law-ketens zodat een ketenfout niet stil achter een correcte endpoint verdwijnt. Dossier-agnostisch; de regelrecht-methode is de vaste taal. Haakt aan op regelrecht-stelselanalyse (engine-tests / meta-check) en regelrecht-audit-products (testcase-scenario's).
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Utilize Shodan, the search engine for Internet-connected devices, to discover exposed assets, vulnerable ports, default credentials, and specific infrastructure configurations using advanced search queries (dorks).
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Use when a task needs the judgment of an allergist/immunologist — interpreting a skin-prick or specific-IgE result against pretest probability, working up a "penicillin allergy" label for delabeling before a needed antibiotic, managing anaphylaxis and biphasic-reaction risk, stepping asthma therapy up or down, or evaluating suspected primary immunodeficiency.
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Firmware development for the Waveshare ESP32-S3-RLCD-4.2 (SKU 33298 / 33507, and the -EN variant) — an ESP32-S3-WROOM-1-N16R8 AIoT board built around a 4.2" fully reflective 300x400 monochrome LCD on a Sitronix ST7305/ST7306, with no backlight, plus an ES8311 speaker codec, an ES7210 dual-microphone ADC, an SHTC3 temperature/humidity sensor, a PCF85063A RTC, a microSD/TF slot on SDMMC, an 18650 holder with battery sensing, BOOT/KEY/PWR buttons and a 2x8 2.54 mm expansion header. Use when working on this board: project setup, platformio.ini, the qio_opi PSRAM memory type, the pin map and which header pins are traps, U8g2 and the ST7305 constructor, a blank or shifted panel, ST7305 low-power mode HPM/LPM, the shared I2C bus and its four slaves, I2S audio directions and the speaker amplifier enable, mounting the TF card, battery voltage, USB CDC console with no UART bridge, flashing over Type-C, deep sleep, or debugging why something on the board does not work.
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