「calling」の検索結果

Detects focal chromatin loops (point interactions / corner-dots) in balanced Hi-C and Micro-C contact maps and aggregates/validates a loop set. Covers de-novo calling with cooltools dots (HiCCUPS-style 4-background local enrichment with lambda-chunked FDR), chromosight (template-correlation), and Mustache (scale-space blob detection); aggregate peak analysis (APA) via cooltools pileup for confirmation; the depth/resolution prerequisite (de-novo needs ~5-10kb resolution = hundreds of millions to billions of valid pairs); consensus across callers and convergent-CTCF support as validation; and differential loops via union anchors plus chromosight quantify. Use when calling chromatin loops or dots from a cooler, deciding whether a map is deep enough to call de-novo vs running APA on known CTCF/cohesin anchors, building an aggregate peak pileup, comparing loops across conditions, or validating loop calls. For HiChIP/PLAC-seq/PCHi-C protein-anchored data use FitHiChIP/MAPS, not dots.

日本語の概要は準備中です。原文の説明を表示しています。

Detects m6A modifications from Oxford Nanopore direct-RNA-sequencing (ONT DRS) signal data using m6Anet (Hendra 2022 *Nat Methods* 19:1590; multiple-instance-learning neural network over DRACH 5-mer signal). Covers the required upstream pipeline (Dorado / Guppy basecalling -> minimap2 transcriptome alignment with `-ax map-ont -uf -k14 --secondary=no` -> nanopolish eventalign with `--scale-events --signal-index` (m6Anet-required) plus `--summary` / `--threads` housekeeping -> m6anet dataprep -> m6anet inference), per-site vs per-read probability interpretation including the `mod_ratio` per-site stoichiometry column, the DRACH-only modeling constraint, minimum-coverage thresholds (20-50 reads per site for stable probability estimates), multi-condition comparison via xPore (Pratanwanich 2021 *Nat Biotechnol* 39:1394), Nanocompore (Leger 2021 *Nat Commun* 12:7198), ELIGOS (Jenjaroenpun 2021 *NAR* 49:e7), and Dorado native modification calling (RNA004 chemistry, 2024+), reference-transcriptome version pinning, the cDNA-vs-DRS chemistry distinction (cDNA-Nanopore CANNOT be used for modification detection), and orthogonal validation against MeRIP / GLORI. Use when calling m6A from ONT DRS without immunoprecipitation, choosing m6Anet vs xPore vs Nanocompore vs ELIGOS vs Dorado native, interpreting per-site `probability_modified` vs `mod_ratio` vs per-read modification probabilities, comparing methylation between conditions from ONT data, deciding between m6Anet for known DRACH sites and Dorado/Remora for genome-wide screening, pinning RNA002 vs RNA004 chemistry and basecaller model versions, or troubleshooting eventalign / dataprep failures.

日本語の概要は準備中です。原文の説明を表示しています。

Detect somatic and germline copy number variants from targeted, exome, and whole-genome sequencing with CNVkit, a read-depth caller that combines on-target and off-target (antitarget) coverage. Covers panel-of-normals construction, flat-reference tumor-only calling, hybrid/amplicon/WGS modes, CBS vs HMM segmentation selection, purity-aware integer calling, and reconciliation against GATK and allele-specific callers. Use when calling CNVs from hybrid-capture panels or exomes, deciding whether CNVkit (depth-only) is the right tool versus an allele-specific caller, building a panel of normals, diagnosing flat-reference false positives, or interpreting log2 ratios into copy-number states.

日本語の概要は準備中です。原文の説明を表示しています。

iOS 26以降のアプリに、端末内で動く言語モデルを組み込むスキル。文章生成・要約、入力からのデータ抽出、独自処理の呼び出し、生成中の画面更新を扱います。

• オフラインの文章生成・要約
• 入力文から項目を抽出したいとき
• AIからアプリ独自の処理を呼ぶ

Automate Callingly tasks via Rube MCP (Composio). Always search tools first for current schemas.

日本語の概要は準備中です。原文の説明を表示しています。

Framework for building LLM-powered applications with agents, chains, and RAG. Supports multiple providers (OpenAI, Anthropic, Google), 500+ integrations, ReAct agents, tool calling, memory management, and vector store retrieval. Use for building chatbots, question-answering systems, autonomous agents, or RAG applications. Best for rapid prototyping and production deployments.

日本語の概要は準備中です。原文の説明を表示しています。

Builds today's call list. Runs lead-triage to rank the top-5 leads most worth calling, supplies talking points from email history, blocks time on the calendar, and drafts follow-up messages. Trigger on "call list," "who am I calling today," "top five to call today," or "block time for calls." An owner who wants leads scored or ranked with no calendar blocks routes to lead-triage directly. Accepts optional count and date arguments.

日本語の概要は準備中です。原文の説明を表示しています。

Automate Callingly tasks via Rube MCP (Composio). Always search tools first for current schemas.

日本語の概要は準備中です。原文の説明を表示しています。

Framework for building LLM-powered applications with agents, chains, and RAG. Supports multiple providers (OpenAI, Anthropic, Google), 500+ integrations, ReAct agents, tool calling, memory management, and vector store retrieval. Use for building chatbots, question-answering systems, autonomous agents, or RAG applications. Best for rapid prototyping and production deployments.

日本語の概要は準備中です。原文の説明を表示しています。

Use when a tool-calling agent does not call a tool, sends wrong arguments, loops without stopping, or needs a function schema. Guides a four-branch diagnosis and five-step schema repair. Do not use for framework-specific, MCP-server, or production-observability questions.

日本語の概要は準備中です。原文の説明を表示しています。

Run or plan deep germline WGS, WES, targeted-panel, cohort, or trio variant-calling workflows with reference-build, known-sites, QC, joint-calling, and annotation checks.

日本語の概要は準備中です。原文の説明を表示しています。

Answer questions about the AI SDK and help build AI-powered features. Use when developers: (1) Ask about AI SDK functions like generateText, streamText, ToolLoopAgent, embed, or tools, (2) Want to build AI agents, chatbots, RAG systems, or text generation features, (3) Have questions about AI providers (OpenAI, Anthropic, Google, etc.), streaming, tool calling, structured output, or embeddings, (4) Use React hooks like useChat or useCompletion. Triggers on: "AI SDK", "Vercel AI SDK", "generateText", "streamText", "add AI to my app", "build an agent", "tool calling", "structured output", "useChat".

日本語の概要は準備中です。原文の説明を表示しています。

Add, review, or fix JavaScript interop in Blazor components. USE FOR: calling JavaScript from Blazor, calling .NET from JavaScript, collocated .razor.js modules, IJSRuntime, IJSObjectReference lifecycle, DotNetObjectReference, ElementReference, timing rules for when JS is available, IAsyncDisposable disposal of JS references, server-side JS interop safety. DO NOT USE FOR: general Blazor component authoring without JS interop needs (use author-component), forms (use collect-user-input).

日本語の概要は準備中です。原文の説明を表示しています。

Customize NVIDIA Nemotron Voice Agent's Generic Pipecat example for healthcare appointment, five-field patient intake, or custom tool-calling workflows without a separate backend.

日本語の概要は準備中です。原文の説明を表示しています。

Azure Communication Services CallingServer (legacy) Java SDK. Note - This SDK is deprecated. Use azure-communication-callautomation instead for new projects. Only use this skill when maintaining legacy code.

日本語の概要は準備中です。原文の説明を表示しています。

Azure AI Agents Persistent SDK for .NET. Low-level SDK for creating and managing AI agents with threads, messages, runs, and tools. Use for agent CRUD, conversation threads, streaming responses, function calling, file search, and code interpreter. Triggers: "PersistentAgentsClient", "persistent agents", "agent threads", "agent runs", "streaming agents", "function calling agents .NET".

日本語の概要は準備中です。原文の説明を表示しています。

Deep learning-based variant calling from long reads using Clair3 for SNPs and small indels. Use when calling germline variants from ONT or PacBio alignments, particularly when high accuracy is needed for clinical or research applications.

日本語の概要は準備中です。原文の説明を表示しています。

Statistical methods for calling hits in CRISPR screens. Covers MAGeCK, BAGEL2, drugZ, and custom approaches for identifying essential and resistance genes. Use when identifying significant genes from screen count data after QC passes.

日本語の概要は準備中です。原文の説明を表示しています。

Call accessible chromatin regions from ATAC-seq data using MACS3 with ATAC-specific parameters. Use when identifying open chromatin regions from aligned ATAC-seq BAM files, different from ChIP-seq peak calling.

日本語の概要は準備中です。原文の説明を表示しています。

Run inference on Fireworks AI — direct, through the Leanmcp AI Gateway proxy (aigateway.leanmcp.com) for observability, or via LiteLLM / the OpenAI SDK / raw HTTP. Covers the endpoint-and-key matrix for each route, serverless vs dedicated deployments and their cold starts, browsing and filtering the model catalogue, reasoning models that return content:null with the answer in reasoning_content, function calling, concurrency and load testing, and runner scripts where many models share one protocol with a --use-proxy toggle. Use this skill whenever the user mentions Fireworks, FIREWORKS_API_KEY, api.fireworks.ai, fireworks_ai/ model ids, accounts/fireworks/models/..., a Fireworks deployment id, the Leanmcp gateway or LEANMCP_API_KEY, app.leanmcp.com observability, or gpt-oss / kimi / glm / qwen / deepseek served on Fireworks; wants to route LLM calls through a proxy for logging or cost tracking; is debugging empty responses, 404s, scope errors, cold starts, or "the gateway isn't working"; wants to pick a Fireworks model with tool calling; or wants to sweep several models over one benchmark. Reach for it even on vague asks like "why is this model returning nothing" or "set up inference for these models" when Fireworks is in play.

日本語の概要は準備中です。原文の説明を表示しています。

Runs voice and meeting infrastructure — phone systems and numbers, emergency calling obligations, conference rooms and their AV, call recording and its retention consequences, and the porting that makes provider changes go badly. Use this to replace a phone system, fix rooms nobody can start a meeting in, meet emergency-calling requirements, port numbers without losing service, or work out why voice quality degrades only sometimes.

日本語の概要は準備中です。原文の説明を表示しています。

Calls m6A peaks from MeRIP-seq / m6A-seq paired IP-vs-input data using exomePeak2 (transcript-aware, GC-bias-corrected Poisson GLM), MeTPeak (HMM over sliding windows), MACS3/MACS2 with --nomodel --broad --keep-dup all (genome-wide broad alternative), and DRACH motif enrichment via HOMER or ggseqlogo as a sanity check (NOT a filter). Covers BED12 vs narrowPeak output, exonic vs intronic peak handling, multi-tool reconciliation (intersection vs union), the m6A-vs-m6Am ambiguity at 5'UTR peaks that antibody methods cannot resolve, and orthogonal validation (miCLIP/GLORI/m6A-SAC-seq/m6Anet). Use when calling peaks from paired IP/input genome BAMs, choosing exomePeak2 (transcript-aware default) vs MACS3 (broad genomic) vs MeTPeak (HMM-smoothed low-coverage), confirming DRACH enrichment as a sanity check on the peak set, reconciling differing peak sets across tools, validating MeRIP peaks against single-base methods, interpreting 5' peaks where m6Am contamination is possible, or recommending a consensus strategy.

日本語の概要は準備中です。原文の説明を表示しています。

Build and analyze pangenomes for prokaryotes (Panaroo, PPanGGOLiN, PEPPAN, GET_HOMOLOGUES, anvi'o pangenomics) and eukaryotes (Minigraph-Cactus, PGGB, vg pangenome graphs). Implement Tettelin core/accessory/cloud genome decomposition (Tettelin 2005), Heap's law open/closed pangenome modeling, gene presence/absence GWAS (Scoary, pyseer), pangenome graph variant calling (vg, PanGenie), and structural-variation graph indexing. Use when assembling species- or genus-level pan-gene catalogs, separating core from accessory/shell/cloud genes, testing gene-content associations with phenotypes, building pangenome graphs from haplotype-resolved assemblies, calling SVs from pangenome graphs, or selecting between bacterial-pangenome and eukaryotic-pangenome workflows.

日本語の概要は準備中です。原文の説明を表示しています。

Cross-method decision tree for calling hits in pooled CRISPR screens. Catalogs statistical models (MAGeCK RRA, MAGeCK MLE, BAGEL2, drugZ, JACKS, Chronos, CERES), experimental designs each is built for, failure modes outside design domain, reconciliation when methods disagree, multiple-testing and effect-size thresholds, the order of operations (count -> QC -> CN-correct -> hit-call -> validate), the second-best-sgRNA conservative rule, and consensus-hit strategy. Use when choosing among MAGeCK / BAGEL2 / drugZ / JACKS / Chronos for a given design, reconciling disagreement across two or three methods on the same screen, deciding whether to require consensus, gating downstream validation by hit-confidence tier, or interpreting unstable hit lists across reruns.

日本語の概要は準備中です。原文の説明を表示しています。