「eth」の検索結果

Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Nat Commun 12:1302), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.

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Analyzes CRISPR drug-modifier (chemogenomic) screens with drugZ (Colic et al. 2019 Genome Med), a bidirectional Z-score method that identifies synthetic-lethal sensitizing genes and resistance-conferring suppressor genes from vehicle vs drug comparisons. Covers vehicle-anchored design (not Day-0), the bidirectional Z math giving greater sensitivity to small-effect hits than MAGeCK / STARS / edgeR / RIGER on drug screens, per-gene sumZ and normZ, synth (sensitizer) vs supp (suppressor) FDR, multi-dose handling, integration with control sgRNAs, and comparison with MAGeCK MLE with dose covariate. Use when running a drug-modifier CRISPR screen, identifying sensitizing or resistance genes for a drug candidate, choosing drugZ vs MAGeCK MLE for chemogenomic analysis, troubleshooting low-effect drug screens where MAGeCK lacks sensitivity, or designing a drug-screen layout (vehicle vs drug arms).

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Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

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Decompose total effects into direct and indirect paths through mediators using mediation, CMAverse 4-way, HIMA/HIMA2 high-dimensional, BAMA, two-step / MVMR mediation, or double-ML medDML. Use when testing whether a molecular phenotype (expression, methylation, protein) mediates a treatment-outcome relationship, decomposing exposure-mediator interaction via VanderWeele 4-way, screening high-dimensional EWAS mediators, or running MR-based mediation when sequential ignorability is implausible.

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Detect and adjust for horizontal pleiotropy in two-sample Mendelian randomization by distinguishing uncorrelated (UHP) from correlated (CHP) pleiotropy and choosing among Egger, MR-PRESSO, MR-RAPS, CAUSE, LHC-MR, LCV, MR-Clust, MR-Mix, and contamination-mixture methods. Use when validating an MR causal claim, running the STROBE-MR sensitivity battery, suspecting a shared heritable confounder, working under weak-instrument or polygenic-exposure regimes, or reconciling discordant estimates across robust methods.

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Test whether two or more traits share a causal variant at a locus using Bayesian colocalization (coloc.abf, coloc.susie, HyPrColoc, moloc, eCAVIAR, SMR/HEIDI, PWCoCo, SharePro). Use when integrating GWAS with eQTL/sQTL/pQTL/mQTL, distinguishing shared causal variants from LD-driven coincidence, handling allelic heterogeneity, choosing between single-causal vs multi-causal methods, picking PP.H4 thresholds, running sensitivity over p12, or harmonising summary statistics for colocalization.

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Find out why something in a running web app does not work, when the console is empty and the code looks correct. Reads the click, the request, the store and the console together and returns the file:line to open. Use when a button does nothing, a form will not submit, data will not load, a page renders blank or stale, a modal will not close, or the user says "it's broken" and the code review says it is fine.

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Discover OPC UA servers, connect MATLAB clients, and browse/navigate server nodes using opcuaserverinfo, the Local Discovery Service (LDS), opcua, connect, setSecurityModel, certificate-trust functions, findNodeById, opcuanode, and Namespace/Children traversal. Use when finding OPC UA servers on the network, querying endpoints and security policies, connecting to a server, authenticating with username/password or certificates, configuring security modes, handling certificate-trust or hostname-mismatch errors, troubleshooting failed connections or empty discovery, inspecting an OPC UA certificate (.der/.pem), browsing an address space, finding nodes by name or NodeId, navigating node hierarchies, invoking method nodes, or batch-processing large namespaces. Trigger on: opcuaserverinfo, LDS, opcua, opc.ua.Client, setSecurityModel, opc.ua.trustServerCertificate, OPC UA certificate, findNodeById, opcuanode, browse OPC UA, find node, OPC UA namespace, OPC UA method node, Industrial Communication Toolbox.

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Set up metrics collection and visualization with Prometheus and Grafana. Configure scrape targets, create PromQL queries, build dashboards, and implement alerting. Use when implementing monitoring, metrics collection, or visualization for applications and infrastructure.

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Verify claims, quotations, citations and references against live primary sources, and produce a reproducible audit record instead of an assertion. Use when asked to: check whether a quotation, verse, statistic, date, attribution or citation is accurate; verify references before publishing a report, thesis, article, slide deck or legal filing; confirm a text against an authoritative database; cross-check a claim across independent sources; build a citation register or verification appendix; find out whether two datasets agree; detect a misattributed or fabricated quotation; or audit a document's existing citations. Source-agnostic by design, with working providers for REST/JSON APIs, scraped HTML, bulk corpora, bibliographic catalogues (OpenLibrary), DOI resolution (Crossref) and reference works (Wikipedia) -- including scriptural tiers (Qur'an.com, sunnah.com and an independent hadith corpus) with full diacritic handling for Arabic, Hebrew, Greek and any script with optional marks. Covers content-addressed matching when sources disagree on numbering, graded match verdicts, byte-level integrity checks, published-string verification, and a catalogue of silent failure modes (identifier divergence, mojibake, scraper blocking, false-positive keyword rules, missing glyphs, unreliable PDF text layers).

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Use when evaluating whether a skill belongs in a library. Preview content, check frontmatter, validate structure, and decide whether to keep, curate, or remove.

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Author or revise a cloud-api-operations recipe (config/source/skills/cloud-api-operations/references/recipes/). Encodes why the long tail of control-plane capabilities is deliberately NOT wrapped as MCP/CLI tools, the API-first admission test for whether a capability deserves a recipe, the negative and positive lists, the fixed recipe structure, the writing rules distilled from writing-for-agents, the user-facing copy red lines, and the product regeneration/verification pipeline. Also routes contributors (and their agents) to the contribution flow and the pre-PR self-test protocol in references/contributing.md. Use when adding a recipe, judging whether a scenario belongs in the recipe set, updating the recipes index, re-checking existing recipes against the admission test, or onboarding someone who wants to submit one.

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Inspect CCAM dashboard-run history and relate live run handles to persisted Claude Code or Codex sessions. Use when finding a prior launched task, checking whether a run is still attached, reviewing start/end status, or deciding whether to resume, view, or relaunch work.

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Benchmark one session (or a small recent set) against the rolling average using Agent Monitor data — cost, total tokens, tool count, and workflow complexity score — and report where each metric lands as a percentile of the population. Tells you whether a session was normal, cheap, or an outlier. Use when judging whether a session was typical or out of band.

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Collect and analyze relevant papers, reports, and reference methods to inform method selection without fabricating references or copying models blindly.

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Extract and maintain global and method-specific mathematical-model assumptions from the problem frame, active method cards, data profile, and risk probes, while leaving necessity and impact judgments to the human modeler.

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Build and risk-screen a compact role-based method shortlist for a mathematical-modeling subquestion. Use after problem framing and data profiling, before model code generation, to propose a main candidate, a usable baseline, and at most one conditional fallback without padding the pool.

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Reduce defensive writing in drafts and revisions by removing unnecessary caveats, disclaimers, hedges, apology-like framing, negative self-limiting statements, and over-explanations while preserving necessary scope, accuracy, safety, legal, ethical, and methodological limits. Use when drafting or revising academic writing, papers, essays, abstracts, introductions, contribution statements, discussions, conclusions, technical explanations, grant writing, product writing, policy writing, professional reports, or any text the user wants to be clearer, stronger, more direct, more concise, more confident, less verbose, less caveated, less apologetic, or less defensive.

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Turn books, PDFs, slides, documents, web pages and text into a source-grounded knowledge base, interactive learning HTML and Markdown in English or Chinese. Use for learning, summaries, explanations, review, quizzes, relationship maps, saving reusable methodology files, retrieving or comparing accumulated methods, and applying them to a real problem. 将材料转为可追溯知识库与交互学习页，支持中英文、关系图、方法论保存/检索/比较及应用；区分材料依据、推断与外部核验。

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Use when a Scenario output must be checked and improved rather than accepted first roll: iterating a generation until it matches the brief, fixing a batch that came back off-brief, retrying failed shots methodically, wiring an automated generate, review, revise loop, or deciding whether to re-prompt, swap references, inpaint, post-process, or change model. Keywords: refine, iterate, critique, review loop, QA, self-correction, verify, acceptance criteria, retry, drift.

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How humans and AI compose in content workflows. Where AI legitimately participates, where humans must own, hybrid workflow patterns, voice ownership preservation, the AI slop problem, disclosure and transparency, team calibration, and the ethics of intellectually honest AI-assisted content production. Triggers on AI content workflow, AI-assisted writing, hybrid content production, AI in editorial, AI slop, AI disclosure, AI usage policy, AI content ethics, voice preservation with AI, team AI calibration. Also triggers when content feels generic despite quality tools, when team AI usage has drifted into inconsistency, or when a regulated or trust-sensitive context requires explicit AI policy.

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A discipline for designing experiments (A/B tests, multivariate, holdouts) so the results actually answer the question you asked. Hypothesis writing, sample size, duration, segment analysis, running discipline, matching a result to a pre-committed decision rule, and the common failure modes that produce confidently wrong shipping decisions. Use this skill whenever the user is planning a test that has not run yet: framing a hypothesis, sizing the sample, setting duration, choosing guardrails, or deciding whether something is worth testing at all. Triggers on design an experiment, experiment plan, A/B test, split test, multivariate test, holdout, experiment hypothesis, sample size, minimum detectable effect, MDE, test duration, guardrail metric, no peeking, pre-committed decision rule, is this worth testing. Use `experimentation-analytics` instead when the test has already run and the question is how to read the result panel.

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Cavekit specification-driven development methodology — the Hunt lifecycle (Draft → Architect → Build → Inspect → Monitor) and how to apply it. Use when starting a Cavekit project, structuring an existing codebase as kits/plans, or routing between sub-skills.

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When the user wants multiple expert perspectives on a marketing question — a simulated board of advisors staffed by legendary marketers (Seth Godin, David Ogilvy, Eugene Schwartz, April Dunford, Rory Sutherland, Alex Hormozi, Byron Sharp, and more). Also use when the user mentions 'marketing council,' 'board of advisors,' 'advisory board,' 'what would Seth Godin say,' 'what would Ogilvy think,' 'channel Hormozi,' 'get multiple perspectives,' 'debate this,' 'have the council review,' 'marketing mentors,' or asks how a famous marketer would approach their problem. The council gives each advisor's take through their documented frameworks, surfaces where they disagree, and synthesizes a recommendation. For executing the winning direction, hand off to positioning, offers, copywriting, ads, or the relevant skill.

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