「guide」の検索結果

Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Compose multi-panel publication figures with patchwork, cowplot, gridExtra (R), or matplotlib GridSpec/subfigures (Python) including shared axes/legends/guides collection, panel labels in Nature/Cell convention, and journal-spec sizing. Covers patchwork ≥1.2.0 axes='collect' feature, Type-42 font embedding, and the cairo_pdf save path. Use when composing 2+ subpanels into a single figure for journal submission.

日本語の概要は準備中です。原文の説明を表示しています。

Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Cell Reports 36:109597), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.

日本語の概要は準備中です。原文の説明を表示しています。

Queries PharmGKB / CPIC / DPWG for drug-gene interactions; calls CYP2D6/CYP2C9/CYP2C19/DPYD/TPMT/NUDT15/UGT1A1/SLCO1B1 star alleles and phenotype with PharmCAT, Cyrius (CYP2D6 structural variants), Aldy, Stargazer; applies Caudle 2020 activity-score translation. Use when implementing pharmacogenomic-guided prescribing, applying CPIC vs DPWG guidance, screening HLA risk alleles for ICI / antiepileptics / abacavir, or interpreting compound TPMT+NUDT15 thiopurine risk.

日本語の概要は準備中です。原文の説明を表示しています。

Perform multiple sequence alignment using MAFFT, MUSCLE5, ClustalOmega, or T-Coffee. Guides tool and algorithm selection based on dataset size, sequence divergence, and downstream application. Use when aligning three or more homologous sequences for phylogenetics, conservation analysis, or evolutionary studies.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

日本語の概要は準備中です。原文の説明を表示しています。

Guides agents through Red-Green-Refactor with runner-captured evidence. Use when running /cc-tdd-cycle, writing tests before implementation, or Global TDD required is yes.

日本語の概要は準備中です。原文の説明を表示しています。

Full-stack Convex development guidelines covering React, Vite, TypeScript, mutations, auth, design system, and documentation practices. Use when building features, writing Convex functions, or making code changes in this project.

日本語の概要は準備中です。原文の説明を表示しています。

Writing style guide for technical content, social media, blog posts, READMEs, git commits, and developer documentation. Optimized to avoid AI detection patterns. Use when writing any content beyond code.

日本語の概要は準備中です。原文の説明を表示しています。

One-time setup that gathers design context for your project and saves it to your AI config file. Run once to establish persistent design guidelines.

日本語の概要は準備中です。原文の説明を表示しています。

Guide for when to use and when not to use return validators in Convex functions. Use this skill whenever the user is writing Convex queries, mutations, or actions and needs guidance on return value validation. Also trigger when the user asks about Convex type safety, runtime validation, AI-generated Convex code, Convex AI rules, Convex security best practices, or when they're debugging return type issues in Convex functions. Trigger this skill when users mention "validators", "returns", "return type", or "exact types" in the context of Convex development. Also trigger when writing or reviewing Convex AI rules or prompts that instruct LLMs how to write Convex code.

日本語の概要は準備中です。原文の説明を表示しています。

Guide for building and refactoring a FastAPI + Prefect + PostgreSQL/pgvector analytics pipeline that extracts insights from chat data using LLM classification, embeddings, UMAP/HDBSCAN clustering, and narrative generation. Use this skill whenever working on this project's codebase — including adding features, refactoring, debugging, creating new endpoints, modifying pipeline stages, or reviewing architecture decisions. Also trigger when the user asks about project structure, stack decisions, async migration, or best practices for this specific system.

日本語の概要は準備中です。原文の説明を表示しています。

To access Anthropic's official brand identity and style resources, use this skill.

日本語の概要は準備中です。原文の説明を表示しています。

You are a senior backend engineer operating production-grade services under strict architectural and reliability constraints. Use when routes, controllers, services, repositories, express middleware, or prisma database access.

日本語の概要は準備中です。原文の説明を表示しています。

This skill enforces Sentry's blog writing standards across every post — whether you're helping an engineer write their first blog post or a marketer draft a product announcement.

日本語の概要は準備中です。原文の説明を表示しています。

To access Anthropic's official brand identity and style resources, use this skill.

日本語の概要は準備中です。原文の説明を表示しています。

Guidelines for modern Avalonia UI layout using Zafiro.Avalonia, emphasizing shared styles, generic components, and avoiding XAML redundancy.

日本語の概要は準備中です。原文の説明を表示しています。

Production-grade Android app development guide covering native (Kotlin/Java), cross-platform (Flutter, RN, KMM), and hybrid architectures.

日本語の概要は準備中です。原文の説明を表示しています。

Orchestrate multiple Antigravity skills through guided workflows for SaaS MVP delivery, security audits, AI agent builds, and browser QA.

日本語の概要は準備中です。原文の説明を表示しています。

Angular performance optimization and best practices guide. Use when writing, reviewing, or refactoring Angular code for optimal performance, bundle size, and rendering efficiency.

日本語の概要は準備中です。原文の説明を表示しています。

API documentation workflow for generating OpenAPI specs, creating developer guides, and maintaining comprehensive API documentation.

日本語の概要は準備中です。原文の説明を表示しています。

Structured guide for setting up A/B tests with mandatory gates for hypothesis, metrics, and execution readiness.

日本語の概要は準備中です。原文の説明を表示しています。

Interactive Flutter Widget implementation assistant that guides you through widget implementation decisions by asking structured questions about state management, widget type (screen/component), and state sharing. Use this skill when you need help determining the appropriate Flutter widget architecture for your implementation.

日本語の概要は準備中です。原文の説明を表示しています。

Jetpack ComposeでUIを実装する時に参照するコーディングガイドライン。 「Android UI」「Compose」「Jetpack」「画面実装」「UIコンポーネント」「レイアウト」と言われたら使う。 Android/Kotlin UI実装のベストプラクティス、アンチパターン、実装パターンを提供する。