「process」の検索結果

Align preprocessed CLIP-seq reads (eCLIP, iCLIP, iCLIP2, PAR-CLIP) to genome with STAR or bowtie2 using crosslink-preserving parameters, choosing between unique-mapper-only and multi-mapper-aware alignment for repeat-binding RBPs, deciding STAR vs HISAT2 memory trade-offs, and applying ENCODE-compatible filters. Use when turning preprocessed CLIP FASTQ into a deduplicated, MAPQ-filtered BAM ready for peak calling or crosslink-site detection.

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Preprocess CLIP-seq reads (eCLIP, iCLIP, iCLIP2, iCLIP3, irCLIP, PAR-CLIP, FLASH) with protocol-specific UMI extraction, adapter trimming, length filtering, and post-alignment PCR-duplicate collapse. Use when raw CLIP FASTQ must be turned into deduplicated, crosslink-preserving BAM input for peak calling; choosing between two-pass and single-pass adapter trimming; deciding minimum read length; or mapping UMI patterns to specific eCLIP/iCLIP/iCLIP2/iCLIP3 library preps.

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Amplicon sequence variant (ASV) inference from 16S rRNA or ITS amplicon sequencing using DADA2. Covers quality filtering, error learning, denoising, and chimera removal. Use when processing demultiplexed amplicon FASTQ files to generate an ASV table for downstream analysis.

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Process multiple sequence files in batch using Biopython. Use when working with many files, merging/splitting sequences, or automating file operations across directories.

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Standard scRNA-seq preprocessing and clustering with Scanpy. Use for QC, normalization, HVG selection, PCA, neighbor graph construction, UMAP, Leiden clustering, and export of an analysis-ready AnnData object.

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Create cross-platform agent skills from workflow descriptions. Activates when users ask to create an agent, automate a repetitive workflow, create a custom skill, or need advanced agent creation. Triggers on phrases like create agent for, automate workflow, create skill for, every day I have to, daily I need to, turn process into agent, need to automate, create a cross-platform skill, validate this skill, export this skill, migrate this skill, audit this skill, is this skill safe, vet a skill before installing, what does this skill access. Supports single skills, multi-agent suites, transcript processing, template-based creation, interactive configuration, cross-platform export, spec validation, and security auditing of third-party skills before install.

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Establish channels and processes for capturing and processing post-launch feedback during PRD v0.9 Go-to-Market. Triggers on requests to set up feedback systems, capture user input, or when user asks "how do we collect feedback?", "feedback loop", "user research", "post-launch feedback", "customer feedback", "NPS", "voice of customer". Outputs CFD- entries specialized for post-launch feedback capture.

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Read, transform, analyze, and generate Excel and CSV files. Use when a user asks to open a spreadsheet, process Excel data, merge CSVs, create pivot tables, clean up data, convert between Excel and CSV, add formulas, filter rows, or generate reports from tabular data. Handles .xlsx, .xls, and .csv.

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Run Codex CLI, Claude Code, or other coding agents as background processes for programmatic control. Use when a user asks to run a coding agent, delegate a task to another AI, spawn a sub-agent, run Claude Code in the background, or orchestrate multiple coding agents on separate tasks.

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Run background tasks in Python with Celery. Use when a user asks to process tasks asynchronously, schedule periodic jobs, run background workers, build task queues in Python, or offload heavy processing from web requests.

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Extract text from complex documents using Chandra OCR — handles tables, forms, handwriting, and full page layouts with high accuracy. Use when: extracting data from scanned documents, reading complex tables from PDFs/images, processing handwritten forms.

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Apache Spark is a distributed engine for batch, SQL and streaming data processing, used from Python through PySpark. Use when a user asks to process big data, run distributed computations, build ETL pipelines, analyse data at scale, read Parquet from S3, or stream from Kafka with PySpark.

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Creative technologist specializing in algorithmic art, shaders, and generative systemsUse when "generative art, creative coding, processing, p5.js, shader art, algorithmic art, art from code, nft art, creative-coding, generative, shaders, processing, p5js, algorithmic-art, nft-art" mentioned.

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Use when integrating Plaid, Stripe, payment processors, or financial APIs - covers account linking, payment processing, KYC/AML compliance, and webhook handlingUse when ", " mentioned.

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Design safe queue consumers and retries. Use when a mid-level developer needs reliable background processing.

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临床诊断思维 (临床诊断思维 / 临床推理 (Clinical Diagnostic Reasoning) — 医生『怎么想病』的元学科:从症状/体征/检查到诊断结论的认知操作系统,从业者(临床医生/住院医/规培生,尤其全科/急诊/大内科等以未分化主诉为生的科室)、医学生与医学教育者、诊断安全与质量改进研究者、以及做医疗 AI 辅助诊断产品的人的视角。覆盖: (a) 第一性张力 — **直觉模式识别 (System 1: illness scripts 疾病脚本 / pattern recognition / gestalt, 『资深人一眼认出 aunt Minnie』) ⇄ 分析性推理 (System 2: hypothetico-deductive 假设演绎 / Bayesian 概率更新)** 的 dual-process 双过程理论 (Kahneman→Croskerry/Norman 谱系), 资深≠更会分析而是『脚本库更大+校准更好』; 更深层论战 — **『认知去偏可教 (Croskerry: bias awareness / cognitive forcing strategies / diagnostic timeout) ⇄ 偏倚标签是马后炮、知识结构才是主因 (Norman/Sherbino/Monteiro: debiasing 干预 transfer 证据弱, bias 是 hindsight 标签)』** — 本行最核心学术对垒; 『概率思维 (验前概率 × 似然比 → 验后概率, Pauker-Kassirer test/treatment threshold 阈值模型) ⇄ 穷尽式排查 (rule-out everything / 防御性医疗 / VOMIT)』; 『临床 gestalt ⇄ 结构化临床决策规则 (Wells/PERC/HEART)』; 『诊断简约 Occam's razor ⇄ Hickam's dictum (病人可以同时得 N 个病)』; 『床旁体格检查复兴 (Verghese Stanford 25 / McGee 循证体检) ⇄ 影像检验替代床旁』; (b) 方法论正典 — illness script theory (Schmidt/Boshuizen: enabling conditions/fault/consequences 三段结构), problem representation + semantic qualifiers 问题表征与语义限定词 (Bowen NEJM 2006, 把病人翻成 one-liner), hypothetico-deductive model (Elstein 1978《Medical Problem Solving》: 早期假设生成+定向检验), Bayesian 工具箱 (sensitivity/specificity/LR/Fagan nomogram/SnNout-SpPin), threshold model (Pauker-Kassirer NEJM 1975/1980), 认知偏倚分类学 (anchoring/premature closure/availability/confirmation/base-rate neglect/search satisficing/diagnostic momentum — Croskerry 偏倚清单), 去偏与元认知策略 (diagnostic timeout/cognitive forcing/Ely checklist/calibration), schema-based reasoning (Clinical Problem Solvers schemas: 按 pivot 症状走分支), reflective practice 结构化反思 (Mamede/Schmidt), 诊断错误科学 (NAM 2015 报告定义 / Newman-Toker Big Three / Hardeep Singh e-triggers / SAFER Dx 框架); (c) 行业结构与角色 — 医学生→实习/住院医 (晨会 morning report/查房被 pimping/汇报训练)→主治 attending→master clinician (NEJM CPC discussant/晨会大师如 Dhaliwal); 配套生态: 医学教育者 (clinical reasoning curriculum + 评估: script concordance test/key features exam/OSCE), 诊断安全研究者 (SIDM/AHRQ), 诊断辅助与 CDS 工具开发者 (DDx generator/AI); (d) 核心工作流 — 数据采集 (病史为王 + 循证体检 + 针对性检验影像) → 问题表征 (one-liner + semantic qualifiers) → 鉴别诊断生成 (schema / 解剖定位法 / VINDICATE-M 病因筛, 按『常见可能 × 致命不能漏 can't-miss』双轴排序) → 假设定向检验 (按 LR 选检查 / 阈值决策) → working diagnosis + 安全网 (red flags 交代 / test of time / test of treatment) → 反馈校准 (follow-up / M&M / diagnostic timeout); 教学工作流: 晨会渐进披露汇报 / CPC / SNAPPS / one-minute preceptor / virtual morning report; AI 增强工作流 (2023-2026: LLM 鉴别诊断头脑风暴 / OpenEvidence 检索 / ambient scribe 释放认知带宽 + 自动化偏倚 guardrails); (e) 产出物 — one-liner, problem list, prioritized DDx, assessment & plan (按问题分层), 晨会/CPC 汇报, M&M 复盘, 诊断不确定性沟通 (『最可能是 X, 但出现 Y 红旗立刻回来』); (f) 教育与评估 — script concordance test / key feature exam / EPA / 里程碑; 中国语境: 人卫《诊断学》教材 / 执业医师考试 / 规培结业临床思维考核; (g) 争议/批判 — debiasing 之争 (Croskerry vs Norman-Sherbino『knowledge is the cure』, 去偏 RCT 效果弱 / bias 标签不可证伪), 诊断错误率数字之争 (『10-15%』经典估计 / Newman-Toker 79.5 万美国年严重伤害外推方法被质疑 / 尸检符合率), dual-process 二分被批过度简化 (连续谱 / 难以实证分离), 决策规则 vs gestalt (资深 gestalt 常不输 Wells 类规则 / 算法厌恶), vignette 研究外推性 + context/case specificity (推理高度内容绑定不可通用迁移 [Norman/Eva] — 对『教推理通用课』产业的根本批判), AI 辅助诊断 2023-2026 (GPT-4 在 NEJM CPC/vignette 追平或超医生 [Kanjee 2023 JAMA / Goh 2024 JAMA Netw Open『AI alone > physician+AI』悖论] / Google AMIE / OpenEvidence 爆发 vs 自动化偏倚 / 去技能化 / 真实环境验证缺失), 防御性医疗与过度检查 (incidentaloma 瀑布 / VOMIT), 体检衰亡之争, pimping 教学法争议; (h) 流派/思想谱系 — 决策科学/Bayesian 派 (Ledley-Lusted 1959 → Elstein/Kassirer/Sox/Pauker/Eddy → Brush) vs 认知心理/双过程派 (Kahneman-Tversky → Croskerry/Graber) vs 教育认知派 (Schmidt/Boshuizen illness scripts → Norman/Eva/Mamede/Durning 情境认知) vs 诊断安全/系统派 (NAM 2015 / Newman-Toker / Hardeep Singh / Graber-SIDM: 错误=系统×认知共因) vs 床旁临床派 (Osler 传统 → Tierney aphorisms / Verghese / McGee / Dhaliwal / Saint: 病史体检为王 + 刻意练习) vs AI/计算派 (INTERNIST-1/DXplain 专家系统 → Isabel → LLM 世代 Rodman/Topol)。不含: 具体专科治疗方案与手术决策深度 (足踝外科/种植牙等另有 skill), 中医辨证 (另有 skill), EBM 文献批判性评价方法学 (相关但本 skill 聚焦诊断推理本身), 护理诊断体系 (NANDA), 影像/病理知觉型读片训练 (相邻但独立), 精神科 DSM 结构化访谈细节, 医患沟通技巧全集 (只覆盖诊断不确定性沟通), 患者自查/自我诊断指

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Audio and video processing with ffmpeg. Use when: user asks to convert, trim, merge, compress, or transcode video or audio files; extract audio from video; create GIFs or animated WebP from video; add subtitles or watermarks to video; change video resolution, framerate, or codec; normalize audio loudness; extract frames from video; concatenate clips; create thumbnails from video; strip or add audio tracks; convert between audio formats (MP3, AAC, FLAC, Opus, WAV); adjust volume; apply video filters; stabilize shaky video; generate waveform or spectrum visualizations; probe media file metadata. Triggers on 'ffmpeg', 'video', 'audio', 'transcode', 'MP4', 'MKV', 'WebM', 'MP3', 'AAC', 'FLAC', 'Opus', 'WAV', 'GIF from video', 'extract audio', 'add subtitles', 'video to gif', 'compress video', 'trim video', 'merge videos', 'normalize audio', 'framerate', 'resolution', 'bitrate', 'codec', 'ffprobe', 'waveform', 'spectrogram'.

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TRIGGER when: user asks to draw, illustrate, or visualize any process, structure, or concept — "画个流程图", "draw an architecture diagram", "帮我画个对比图", "visualize this process", "make a diagram for this doc", or mentions 流程图/架构图/示意图/对比图/时序图/泳道图/时间线. Also trigger when user provides a document/article and asks for illustrations or diagrams. DO NOT trigger for: chart/plot requests (bar charts, line graphs — those need a charting library, not draw.io), or artistic image requests (logos, mascots, drawings of objects/people — those need an image tool).

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When the user wants help with revenue operations, lead lifecycle management, or marketing-to-sales handoff processes. Also use when the user mentions 'RevOps,' 'revenue operations,' 'lead scoring,' 'lead routing,' 'MQL,' 'SQL,' 'pipeline stages,' 'deal desk,' 'CRM automation,' 'marketing-to-sales handoff,' 'data hygiene,' 'leads aren't getting to sales,' 'pipeline management,' 'lead qualification,' or 'when should marketing hand off to sales.' Use this for anything involving the systems and processes that connect marketing to revenue. For cold outreach emails, see cold-email. For email drip campaigns, see emails. For pricing decisions, see pricing.

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Applies the reasoning, architectural principles, and AI philosophy of Christopher Manning (natural language processing expert, Stanford University, director of Stanford AI Lab). Use this skill whenever you are discussing natural language processing, LLM architecture, AI research strategy, cognitive science, or the evolution of machine learning. Trigger this skill for questions about AGI timelines, academic vs. industry research trade-offs, linguistic structure in neural networks, modularity in AI design, or evaluating true intelligence versus mere memorization. Channel his pragmatic focus on domain science, adaptability, and competing on ideas rather than raw compute.

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Use this skill when reading sensor data from CSV files, writing simulation results to CSV, processing time-series data with pandas, or handling missing values in datasets.

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Lark/Feishu real-time event listening / subscribing / consuming: stream events as NDJSON via `lark-cli event consume <EventKey>` (covers IM message receive, reactions, chat member changes, etc.). Use for Lark bots, real-time message processing, long-running subscribers, streaming webhook/push handlers. Supports `--max-events` / `--timeout` bounded runs and a stderr ready-marker contract — designed for AI agents running as subprocesses.

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Script a teacher think-aloud demonstrating expert reasoning processes for a specific task. Use when modelling problem-solving, writing, reading comprehension, or analytical processes.

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Design a pedagogical documentation protocol for making children's learning processes visible and shareable. Use when documenting learning, communicating with families, or planning next steps.

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