「recovery」の検索結果

Designs and analyzes in vivo CRISPR screens in animal tumor models, organoids, and immune-cell adoptive transfers. Covers bottleneck math (250x cells/sgRNA requires ~25M cells implanted; impossible for most syngeneic models, forcing focused libraries), focused library design (Manguso 2017 Nature 547:413 immune screen; Chen 2015 tumor screens), CRISPR-StAR intrinsic-control screening (Uijttewaal 2025 Nat Biotechnol 43:1848), clonal-dynamics-limited detection, tumor-explant DNA recovery, syngeneic vs xenograft vs PDX considerations, and the relationship to downstream MAGeCK / drugZ analysis. Use when designing in vivo CRISPR screens for tumor / immune / metastasis biology, choosing focused vs genome-wide for animal models, addressing bottleneck-induced clonal collapse, picking the syngeneic / xenograft / PDX model, integrating in vivo with in vitro results, or applying CRISPR-StAR for animal experiments.

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Plan, execute, and document validation, verification, and transfer of analytical procedures under the governing framework - ICH Q2(R2) and Q14, USP <1220>/<1225>/<1226>, ICH M10 bioanalytical, CLSI EP, or ISO/IEC 17025. Use for HPLC, LC-MS/MS, GC, CE, ICP-MS, dissolution, qNMR, qPCR, NIR, and ligand binding or cell-based assays whenever the question is whether a procedure is fit for its intended purpose. Triggers include "method validation", "analytical method validation", "AMV", "validation protocol", "acceptance criteria", "linearity", "reportable range", "accuracy and precision", "repeatability", "intermediate precision", "recovery", "LOD", "LOQ", "detection limit", "quantitation limit", "specificity", "robustness", "method transfer", "method comparison", "Deming", "Passing-Bablok", "Bland-Altman", "equivalence testing", "OOS investigation", "ICH Q2", "Q2(R2)", "Q14", "USP 1225", "ICH M10", "incurred sample reanalysis", "ISR", "CLSI EP", and any request to show that an assay works.

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Test a changed user interaction for realistic sequence failures such as double submission, stale responses, navigation, and recovery; use for interaction QA rather than general code review.

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Implementing the `Idempotency-Key` HTTP header pattern (Stripe-pioneered, IETF-standardizing) so retries don't double-charge or duplicate side effects — full Postgres schema with recovery-point state machine, fingerprint check, in-progress lock with 409 response, and 24h retention. Grounded in Stripe blog/docs, the IETF httpapi WG draft, and Brandur Leach's canonical implementation.

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Git branching strategies, conflict resolution, rebase workflows, monorepo patterns, and advanced operations like cherry-pick, bisect, and reflog recovery. Activate on "git", "branching strategy", "merge conflict", "rebase", "cherry-pick", "monorepo git", "git bisect", "git reflog", "squash commits". NOT for GitHub Actions CI/CD (use github-actions-pipeline-builder), GitHub API/webhooks, or PR review processes (use code-review-checklist).

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Design error handling strategies for TypeScript and Python applications — exception hierarchies, Result/Either types, retry patterns, error boundaries, and structured error logging. Use when designing error handling architecture, choosing between exceptions and Result types, implementing retry logic, or building error recovery flows. Activate on "error handling", "exception hierarchy", "Result type", "retry pattern", "circuit breaker", "error boundary", "Pokemon exception". NOT for debugging specific runtime errors, logging infrastructure setup, or monitoring/alerting configuration.

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Benchmark multi-agent coordination systems with experiment design, power analysis, human rating protocols, bootstrap confidence intervals, and reproducible reporting. Use for salvage latency, recovery fidelity, coordination overhead, and protocol comparison studies. NOT for ML model benchmarking, web-product A/B testing, survey design, or general-purpose data science.

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Digital equity and trauma-informed design for marginalized populations. Activate on "accessibility", "offline-first", "trauma-informed", "reentry", "recovery population", "shared device", "unstable phone", "digital equity", "design justice", "low-literacy", "intermittent access". NOT for general UX, marketing optimization, or enterprise SaaS design.

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Decides HOW to mutate a DAG when a node fails, quality is below threshold, or new information changes the plan. Selects from mutation strategies (add node, replace agent, fork paths, loop back, downgrade model) based on failure type, cost budget, and execution history. Activate on "DAG failed how to fix", "mutation strategy", "replan on failure", "adaptive DAG", "recovery strategy", "what to do when node fails". NOT for detecting failures (use dag-quality), executing mutations (use dag-planner), or runtime execution (use dag-runtime).

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Detect crisis signals in user content using NLP, mental health sentiment analysis, and safe intervention protocols. Implements suicide ideation detection, automated escalation, and crisis resource integration. Use for mental health apps, recovery platforms, support communities. Activate on "crisis detection", "suicide prevention", "mental health NLP", "intervention protocol". NOT for general sentiment analysis, medical diagnosis, or replacing professional help.

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Fundamental patterns for effective agentic behavior. Teaches decomposition, tool orchestration, error recovery, context management, quality self-assessment, and knowing when to stop. Model-agnostic principles that make any agent more effective regardless of domain. Activate on: "how should I structure this agent", "agentic workflow", "agent patterns", "multi-step task", "tool orchestration", "/agentic-patterns", "decompose this", "agent best practices", "chain of actions", "when should the agent stop", "agent loop design". NOT for: creating agent infrastructure (use agent-creator), building DAGs (use windags-architect), specific tool implementation.

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This skill recommends obtaining serum free T4 and prolactin levels within 1–2 weeks after transsphenoidal surgery (TSS) for Cushing's syndrome to screen for overt hypopituitarism. Use in postoperative Cushing's syndrome patients during the early recovery window when evaluating for new pituitary hormone deficiencies.

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This skill recommends re-evaluating the need for treatment of other pituitary hormone deficiencies in the postoperative period following surgical intervention for Cushing's syndrome. It is triggered when assessing a post-op CS patient for ongoing pituitary function, evaluating for hypopituitarism, or checking HPA axis recovery with morning cortisol/ACTH stimulation tests and pituitary MRI within 1–3 months.

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This skill recommends measuring serum sodium several times during the first 5–14 days after transsphenoidal surgery to detect and manage electrolyte disturbances such as postoperative hyponatremia and diabetes insipidus. Use when managing a post-TSS Cushing’s syndrome patient in the early recovery phase; triggers include postoperative day 5–10 hyponatremia, polyuria, fluid intake‑output imbalance, or suspected diabetes insipidus.

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Guides systematic root-cause debugging. Use when tests fail, builds break, behavior doesn't match expectations, or you encounter any unexpected error. Use when you need a systematic approach to finding and fixing the root cause rather than guessing.

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Expert-level database systems theory covering relational model, query processing and optimization, transaction management, concurrency control, recovery, and NoSQL systems.

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[Utilities] Use when you need to save analysis context to a checkpoint file for recovery (user-facing alias for memory-management Part 1 CHECKPOINT_CREATE).

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Use when designing or implementing states for AI agent tasks, background jobs, local model runs, queues, and notifications — one shared set of states, only legal transitions, and a recovery path out of every failure.

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Use when a macOS app needs a TCC permission or touches private data — the map of which API, Info.plist key, and entitlement each permission needs, pre-prompt explanation patterns, denied-state recovery with System Settings deep links, and honest privacy copy.

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Mid-run failure recovery for disease-market-sizing-orchestration. Inspects .cache/<slug>/ to determine which phase last completed and which phase to run next. Use when orchestration was interrupted and user wants to continue without restarting from phase 1.

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Build incident response timelines and report packs from event logs. Use for detection-to-recovery reporting, phase tracking, and stakeholder-ready incident summaries.

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Structured self-debugging workflow for AI agent failures using capture, diagnosis, contained recovery, and introspection reports.

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Responds to malware infections across enterprise endpoints by identifying the malware family, determining infection vectors, assessing spread, and executing eradication procedures. Covers the full lifecycle from detection through containment, analysis, removal, and recovery. Activates for requests involving malware response, malware eradication, trojan removal, worm containment, malware triage, or infected endpoint remediation.

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Builds a structured ransomware incident response playbook aligned with the CISA StopRansomware Guide and NIST Cybersecurity Framework. Covers preparation, detection, containment, eradication, recovery, and post-incident phases with actionable checklists. Activates for requests involving ransomware response planning, CISA compliance, incident response playbook creation, or ransomware preparedness assessment.

日本語の概要は準備中です。原文の説明を表示しています。