Work with FASTQ quality scores using Biopython. Use when analyzing read quality, filtering by quality, trimming low-quality bases, or generating quality reports.
日本語の概要は準備中です。原文の説明を表示しています。
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概要と使いどころ
Work with FASTQ quality scores using Biopython. Use when analyzing read quality, filtering by quality, trimming low-quality bases, or generating quality reports.
日本語の概要は準備中です。原文の説明を表示しています。
Filter and prioritize variants by pathogenicity, population frequency, and clinical evidence for rare disease analysis. Use when identifying candidate disease-causing variants from exome or genome sequencing.
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Process Hi-C read pairs using pairtools. Parse alignments, filter duplicates, classify pairs, and generate contact statistics from Hi-C sequencing data. Use when processing raw Hi-C read pairs.
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Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller. Covers model selection, GPU acceleration, modified base detection, and quality filtering. Use when processing raw Nanopore data before alignment. Guppy is deprecated; use Dorado for all new analyses.
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Preprocesses cell-free DNA sequencing data including adapter trimming, alignment optimized for short fragments, and UMI-aware duplicate removal using fgbio. Applies cfDNA-specific quality thresholds and fragment length filtering. Use when processing plasma cfDNA sequencing data before downstream analysis.
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Query gnomAD for population allele frequencies to assess variant rarity. Use when filtering variants by population frequency for rare disease analysis or determining if a variant is common in the general population.
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Detect and correct for horizontal pleiotropy in Mendelian randomization analyses using MR-PRESSO for outlier removal, MR-Egger regression for directional pleiotropy, and Steiger filtering for variant directionality. Use when validating MR results, detecting pleiotropic instruments, or running sensitivity analyses for causal inference.
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Screen US stocks for high-quality dividend opportunities combining value characteristics (P/E ratio under 20, P/B ratio under 2), attractive yields (3% or higher), and consistent growth (dividend/revenue/EPS trending up over 3 years). Supports two-stage screening using FINVIZ Elite API for efficient pre-filtering followed by FMP API for detailed analysis. Use when user requests dividend stock screening, income portfolio ideas, or quality value stocks with strong fundamentals.
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Screen US stocks for Stockbee-style short-term Momentum Burst setups using 4% breakout, dollar breakout, range expansion, volume expansion, prior range contraction, close-location, failure filters, and risk-distance scoring. Use when the user asks for Stockbee, Pradeep Bonde, momentum burst, 4% breakout, range expansion, dollar breakout, short-term swing momentum candidates, or 3-5 day burst setup review.
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Fetch upcoming economic events and data releases using FMP API. Retrieve scheduled central bank decisions, employment reports, inflation data, GDP releases, and other market-moving economic indicators for specified date ranges (default: next 7 days). The script outputs raw JSON or text; the assistant filters, assesses impact, and generates the Markdown report.
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Fetch official FXMacroData macro release-calendar events for trade planning, macro regime checks, and event-risk filters. Use before CPI, NFP, GDP, PCE, retail sales, PMI, and central-bank decision windows.
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Use when the user asks to "generate subject line variants", "pre-score my subject lines", or "will this subject get truncated / trigger spam filters"; produces a labeled subject + preheader variant set and a per-variant heuristic pre-score card — spam-trigger flags, length/truncation across desktop + mobile, emoji-count, and the inbox preview render (from-name + subject + preheader) — before any test is run. Not for the body copy or CTA — use email-creative-builder; not for the A/B test design or significance read — use send-experiment-designer; not for the profile-weighted EQS or the S1/S2/N1/D1 vetoes — use email-quality-auditor. 邮件主题行生成/主题行预打分/截断与垃圾词检查
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Configures AWS WAF to filter web traffic: creating web access control lists (web ACLs) on CloudFront, Application Load Balancers, API Gateway, and AppSync; AWS Managed Rules tuned in Count mode; rate-based rules for HTTP floods; IP set and geographic match rules; Bot Control (Common and Targeted); turning bot labels into a confidence signal; stripping spoofed inbound x-amzn-waf-* headers; recovering the real client IP behind a CDN; Fraud Control (account takeover and account creation fraud prevention); and logging and request sampling. Use when the user wants to protect a web application or API from common exploits, bots, credential stuffing, fake-account creation, or HTTP floods at the application layer (layer 7). Routes to the right per-task procedure in references. Do NOT use for L3/L4 DDoS protection (shieldadvanced skill), multi-account WAF rollout (firewallmanager skill), CloudFront configuration (cloudfront skill), or Route 53 health checks or records (route53 skill).
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Performs a full AWS Well-Architected Framework review evaluating every framework question across all pillars discovered from the live AWS documentation by analyzing code, IaC, and configurations to produce evidence-backed findings with Eisenhower-prioritized remediation. Supports full reviews (every framework best practice with BP ID citations), quick reviews (question-level), pillar-scoped reviews, score-mode reviews (a maturity scorecard with per-pillar scores and filtered findings), and lens-specific reviews using lenses discovered from the live AWS documentation. Triggers on mentions of Well-Architected review, WA review, WAR, pillar assessment, architecture review across pillars, workload assessment, cloud readiness evaluation, or a Well-Architected score, grade, or scorecard request. Does not apply to single-pillar deep-dives, learning WA concepts, ADRs, or migration readiness assessments.
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Routes AWS networking requests to the correct service skill for implementation. Covers Route 53 (DNS, health checks, routing policies, Resolver, DNS Firewall), CloudFront (caching, edge, OAC, mTLS, signed URLs), Transit Gateway (multi-VPC hub, segmentation, centralized egress), Direct Connect (hybrid link, DX Gateway, MACsec), Site-to-Site VPN (IPsec tunnels, static or BGP), WAF (web ACLs, AWS Managed Rules, rate-based rules, Bot and Fraud Control), and Shield Advanced (L3/L4 DDoS). Applicable when creating, configuring, troubleshooting, or designing across these services, choosing between them, or diagnosing connectivity or traffic-filtering issues. Not for VPC subnets and route tables, load balancers, VPC endpoints, PrivateLink, API Gateway, IAM policy logic, container or serverless networking, or IaC authoring.
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Implement or review a single-index Algolia search contract with filters, facets, pagination, and highlighting. Use when building product or content search against the JavaScript v5 client. Trigger with "Algolia search workflow", "add facets", or "searchSingleIndex".
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Org-grade attack-surface mapping: given a company's legal identity, discover its ENTIRE owned internet footprint — corporate family -> owned domains -> owned netblocks/ASN -> live assets — with attribution discipline, not just DNS breadth. The org-first attribution pyramid (legal entity -> LEI/registration -> corporate family -> owned domains -> owned netblocks/ASN -> live assets). Corporate-identity resolution via the GLEIF LEI API (legal name -> LEI, exact-LEI direct-children expansion, downward-only depth-capped BFS, NEVER a name re-resolution), SEC-EDGAR full-text search + Exhibit-21 subsidiary entity names, OpenCorporates entity corroboration, Wikidata SPARQL corporate graph (P856/P355/P749/P1830). Domain attribution via reverse-WHOIS (WhoisXML preview-then-purchase quota guard, SecurityTrails associated-domains — both paid), crt.sh O= certificate-transparency organization pivot (keyless), infrastructure correlation (shared NS/MX/SaaS-TXT, netblock membership, reverse-DNS PTR), and the independent-evidence combiner (1-prod(1-w_i), rule of three, OwnerTier NONE/WEAK/MODERATE/STRONG/CONFIRMED) — discover-only `related:` candidates are NEVER auto-scanned. Netblock/ASN attribution via org-first RIR queries (ARIN Whois-RWS org-handle search, RIPE DB organisation + inverse-org search) that recover 'dark netblocks' with no DNS link to the seed, ASN discovery (RIPEstat + BGPView union), the HYPERSCALER-SCOPE GUARD (never attribute a whole AWS/GCP/Azure/Cloudflare announced range to a tenant — keep only the seed-containing block, tag shared_hosting_cdn), and org-identity-seeded internet-scan-index queries (Shodan/Censys/ZoomEye/FOFA/BinaryEdge org: filters + an always-on keyless crt.sh fallback). Promote-to-scan triage ranks forgotten discover-only netblocks by remote-access exposure (gateway-vendor/KEV/control-plane/datastore port scoring) into an operator queue. Attribution confidence rubric + anti-patterns (namesake grafting via GLEIF name re-resolution, single-signal ownership, hyperscaler over-attribution, privacy-WHOIS pivot poisoning, RIR org-name collisions). Passive/keyless-first OSINT only — every paid-key dependency is enrichment on top of a keyless core, never a hard requirement. Use when mapping an organization's full corporate-family internet footprint, resolving a legal entity to its LEI/subsidiaries, discovering domains/netblocks/ASNs an org owns beyond its one seed domain, auditing M&A/shadow-IT sprawl, or scoping an engagement that starts from a company NAME rather than a domain.
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Traffic analysis and PCAP forensics playbook. Use when analyzing network captures including Wireshark filters, protocol analysis (HTTP/DNS/FTP/SMTP/USB/WiFi), data extraction, covert channel detection, PCAP repair, TLS decryption, and tshark command-line analysis.
日本語の概要は準備中です。原文の説明を表示しています。
Linux security mechanism bypass playbook. Use when facing restricted bash/rbash, read-only or noexec filesystems, AppArmor, SELinux, seccomp filters, or audit logging that must be evaded during post-exploitation.
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Sandbox escape playbook. Use when breaking out of Python sandbox, Lua sandbox, seccomp filter, chroot jail, container/Docker, browser sandbox, or namespace isolation to achieve unrestricted code execution or file access.
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SQL injection playbook. Use when input reaches SQL queries, authentication logic, sorting, filtering, reporting, or DB-specific blind and out-of-band execution paths.
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Advanced prototype pollution playbook — server-side RCE, client-side gadgets, filter bypasses, and detection techniques. Companion to ../prototype-pollution/ for basics. Use when you've confirmed pollution and need to escalate to code execution or find framework-specific gadgets.
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NoSQL injection playbook. Use when MongoDB-style operators, JSON query objects, flexible search filters, or backend query DSLs may allow data or logic abuse.
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HTTP Parameter Pollution (HPP): duplicate query/body keys parsed differently by servers, proxies, WAFs, and app frameworks. Use when filters and application layers disagree on which value wins, enabling bypass, SSRF second URL, logic abuse, or CSRF token confusion.
日本語の概要は準備中です。原文の説明を表示しています。