「spr」の検索結果

Identifies essential genes from CRISPR-Cas9 fitness screens using BAGEL2 (Kim & Hart 2021 Genome Med), a Bayesian classifier scoring per-gene Bayes Factors via log-likelihood ratios over per-sgRNA fold changes, calibrated against CEGv2 core-essentials (Hart 2017 G3, ~684 genes) and NEGv1 non-essentials (Hart 2014, ~927 genes). Covers the fc + bf + pr workflow, the linear-extrapolation improvement over BAGEL1 truncation, multi-target off-target correction, tumor-suppressor sensitivity (BAGEL2 detects enrichment), and BF calibration (BF >6 ≈ 90% posterior per Hart 2017; ~5% FDR by BAGEL convention). Use when classifying essential vs non-essential genes, calibrating BAGEL2 thresholds against PR curves, identifying tumor suppressors alongside essentials, comparing BAGEL2 hits to MAGeCK / drugZ, or generating publication-quality essentiality calls.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes base-editing screens for variant function. Covers library design (Hanna 2021 ClinVar-scale CBE screen benchmarked on BRCA1/2, Cuella-Martin 2021 DDR saturation), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end; 4-7 for ABE7.10), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

日本語の概要は準備中です。原文の説明を表示しています。

Cross-method decision tree for calling hits in pooled CRISPR screens. Catalogs statistical models (MAGeCK RRA, MAGeCK MLE, BAGEL2, drugZ, JACKS, Chronos, CERES), experimental designs each is built for, failure modes outside design domain, reconciliation when methods disagree, multiple-testing and effect-size thresholds, the order of operations (count -> QC -> CN-correct -> hit-call -> validate), the second-best-sgRNA conservative rule, and consensus-hit strategy. Use when choosing among MAGeCK / BAGEL2 / drugZ / JACKS / Chronos for a given design, reconciling disagreement across two or three methods on the same screen, deciding whether to require consensus, gating downstream validation by hit-confidence tier, or interpreting unstable hit lists across reruns.

日本語の概要は準備中です。原文の説明を表示しています。

Agile product ownership toolkit for Senior Product Owner including INVEST-compliant user story generation, sprint planning, backlog management, and velocity tracking. Use for story writing, sprint planning, stakeholder communication, and agile ceremonies.

日本語の概要は準備中です。原文の説明を表示しています。

Expert-level machine design covering shafts, bearings, gears, fasteners, springs, clutches, brakes, and mechanical power transmission systems.

日本語の概要は準備中です。原文の説明を表示しています。

Expert-level molecular biology knowledge. Use when working with DNA replication, transcription, translation, gene regulation, cloning, PCR, sequencing, CRISPR, epigenetics, or molecular techniques. Also use when the user mentions 'DNA replication', 'transcription', 'translation', 'gene expression', 'PCR', 'cloning', 'restriction enzyme', 'gel electrophoresis', 'Western blot', 'CRISPR', 'epigenetics', 'promoter', 'enhancer', or 'RNA splicing'.

日本語の概要は準備中です。原文の説明を表示しています。

Manage significant changes during sprint execution. Use when the user says "correct course" or "propose sprint change"

日本語の概要は準備中です。原文の説明を表示しています。

Guides continuous improvement and change management for SMEs adopting AI. Applies Marginal Gains philosophy, Kaizen principles, and structured sprint methodologies to organizational transformation. Use when user asks about change management, continuous improvement, AI adoption strategy, team resistance, transformation roadmaps, or improvement sprints.

日本語の概要は準備中です。原文の説明を表示しています。

Run a two-agent proxy approval council for autonomous sprint gates. Use when a sprint needs delegated approval for human gates such as deploy, live mutation, push, cleanup, skip, or shortened watch decisions, while requiring two independent agents to reach consensus that the human principal would approve under written policy and preferences.

日本語の概要は準備中です。原文の説明を表示しています。

Kit de ferramentas de product ownership ágil para Senior Product Owner, incluindo geração de histórias de usuário compatíveis com INVEST, planejamento de sprints, gerenciamento de backlog e rastreamento de velocidade. Use para escrita de histórias, planejamento de sprints, comunicação com stakeholders e cerimônias ágeis.

日本語の概要は準備中です。原文の説明を表示しています。

Use quando o usuário menciona issues do Jira (ex: "PROJ-123"), pergunta sobre tickets, quer criar/visualizar/atualizar issues, verificar status do sprint ou gerenciar seu workflow no Jira. Ativa em palavras-chave como "jira", "issue", "ticket", "sprint", "backlog" ou padrões de chaves de issue.

日本語の概要は準備中です。原文の説明を表示しています。

Validate Commodore 64 VIC-II emulation in EMULATORS, including $D000-$D3FF register behavior, PAL/NTSC timing, raster counters, badlines, BA/AEC bus stealing, video matrix and bitmap fetches, sprites, collisions, borders, IRQs, color RAM usage, and display modes. Use when Codex reviews or edits VICII, Screen, Memory/PLA, raster, sprite, video, or cycle-level behavior for C64 classes.

日本語の概要は準備中です。原文の説明を表示しています。

Detect lateral movement in network traffic using Zeek (formerly Bro) log analysis. Parses conn.log, smb_mapping.log, smb_files.log, dce_rpc.log, kerberos.log, and ntlm.log to identify SMB file transfers, NTLM account spray activity, remote service execution, and anomalous internal connections.

日本語の概要は準備中です。原文の説明を表示しています。

Tiefe Pflegerunde für ein veröffentlichtes GitHub-Repository (Stufe 2): enthält den vollständigen surface-after-care-Durchlauf und ergänzt ihn um drei teure Schritte — rechtliche Ersteinschätzung über die Law-Checker mit Wiedervorlage nach einem Jahr (Gutachten bleibt gitignored im Repo), Querverweise zu verwandten Repos über ALLE Organisationen hinweg sowie das Nachziehen aller Sprachen auf App-Ebene, nicht nur in der Doku. Nutze diesen Skill bei "full after care", "deep after care", "tiefe Repo-Pflege", "große Runde", "Repo grundlegend durchgehen", wenn ein Repo länger nicht geprüft wurde, vor größeren Releases oder wenn rechtliche Relevanz, Querverweise oder Mehrsprachigkeit ausdrücklich Thema sind. Für die günstige, oft wiederholte Runde stattdessen surface-after-care; für die Erstveröffentlichung github-repo-care.

日本語の概要は準備中です。原文の説明を表示しています。

Plant Sprachaufnahme, Transkription und Sprachausgabe mit optionalen, austauschbaren Werkzeugen.

日本語の概要は準備中です。原文の説明を表示しています。

Turns a schedule extract (milestone list or planning tool export) and the period's status notes into a DRAFT plain-language slip explanation: which milestones and activities slipped and by how many working days, arithmetic shown, why per the notes as verbatim fragments with source and date, what each slip pushes next per the extract, and the decision each slip needs framed as a question with the options the notes propose and the owner the governance documents name, every missing fact marked UNKNOWN. Use when the user asks to "explain the schedule slip", "what slipped this month and why", "write the schedule commentary for the steering pack", "put the delay in plain language for the sponsor" or "what decisions do these slips need". Do not use for the weekly status report, use project-status-tracker instead; for cost variances, use budget-variance-explainer; for one sprint's carry-over, use sprint-review-summary. Drafts for human review; never approves, authorises or signs off.

日本語の概要は準備中です。原文の説明を表示しています。

Synthesises retrospective notes, closing reports and post-implementation reviews into a DRAFT lessons-learned document: themed lessons with source counts, anonymised quoted evidence graded by strength, repeats against a prior register, contradictions, and a recommended owner with a stated basis for each follow-up. Names no individual or vendor and assigns no blame. Use when the user asks to "compile the lessons learned", "write up the retrospective", "theme the close-out notes", "what did we learn across these projects" or "refresh the lessons register". Do not use for a single sprint's review, use sprint-review-summary instead; for an incident investigation, use incident-postmortem-drafter; for customer feedback themes, use customer-feedback-theme-synthesis. Drafts for human review; never approves, authorises or signs off.

日本語の概要は準備中です。原文の説明を表示しています。

Reads one spreadsheet, CSV export or pasted table together with the user's question and drafts an insight pack: a data profile, observations cited to named columns and values and framed as leads, caveats, and suggested charts and follow-up analyses. Every figure carries the label "as read, verify in source"; correlation is never stated as causation. Use when the user asks to "profile this spreadsheet", "what does this data say", "find anomalies in this extract", "give me a first read on this CSV" or "summarise this table". Do not use for a purchase order log or spend extract, use purchase-order-anomaly-review instead; for actuals versus budget, use budget-variance-explainer instead; for a report that arrives by email on a schedule, use report-attachment-analyzer instead. Drafts for human review; never approves, authorises or signs off.

日本語の概要は準備中です。原文の説明を表示しています。

Marketing project management and execution excellence for B2B SaaS campaigns. Use this skill when coordinating multi-channel campaigns, allocating team resources, planning agile marketing sprints, running QBRs with clients, managing project timelines, balancing marketing workload, or implementing marketing operations scrum. Also triggers on: campaign coordination, resource allocation, agile marketing, sprint planning, client success, QBR, project timeline, marketing ops scrum, workload balancing.

日本語の概要は準備中です。原文の説明を表示しています。

スプリントレビューを実行する。GitHub Issues から完了機能サマリー・未完了分析・レポート生成・Slack通知を自動化する

Use when working with Azure Boards Deep — deep Azure Boards management covering work items, sprints, backlogs, queries, and team velocity analytics. Use when performing deep audits of Azure DevOps Boards, analyzing sprint health, reviewing backlog grooming, or assessing team capacity and velocity across Azure DevOps projects.

日本語の概要は準備中です。原文の説明を表示しています。

Excel (.xlsx) manipulation via MCP server. Use for creating workbooks, formatting cells, writing formulas, building charts, pivot tables, data analysis, or any task involving Excel spreadsheets.

日本語の概要は準備中です。原文の説明を表示しています。

Expert en biotechnologie (bioinformatics, genomics, CRISPR, clinical trials, FDA)

日本語の概要は準備中です。原文の説明を表示しています。

Expert en biotechnologies avancées (bioinformatics, genomics, CRISPR, drug discovery, DZ research)

日本語の概要は準備中です。原文の説明を表示しています。