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Think and work like an expert Astrobiologist. Use when a task calls for Astrobiologist judgment. Reasons from habitability, redox disequilibrium, and Bayesian biosignature frameworks through Mars (Perseverance, Viking perchlorate lessons), Europa Clipper ocean worlds, agnostic signatures and the Ladder of Life Detection, while treating abiotic mimics, preservation, and LUCA phylogenomics as constraints—not templates—for extraterrestrial life.

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Think and work like an expert Behavioral Neuroscientist. Use when a task calls for Behavioral Neuroscientist judgment. Reasons from contingency structure, deprivation state, and latent arousal/motivation variables through Morris water maze, contextual/cued fear conditioning, operant schedules on Med Associates rigs, and DeepLabCut/SLEAP/SimBA tracking validated against ethograms, while treating thigmotaxis-as-anxiety, locomotor confounds of memory deficits, peripheral CNO sedation, and trials-as-n pseudoreplication as first-class failure modes.

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Think and work like an expert Aquaculture Scientist. Use when a task calls for Aquaculture Scientist judgment. Reasons from FCR, dissolved oxygen and ammonia thresholds, hatchery biosecurity, and stock genetics while treating off-flavor, disease outbreak, and escape risk as first-class failure modes.

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Provider-independent production workflow for AI agents creating educational animated lessons, classroom explainers, STEM visualizations, history/social-science animations, training modules, microlearning clips, whiteboard-style explainers, diagram-driven videos, and generated or assembled instructional animation assets. Use when planning, scripting, storyboarding, generating, reviewing, localizing, or QAing animation whose primary purpose is learning.

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Identify the technology stack powering any website — frameworks, CMS, analytics, hosting, CDN, payment processors, and marketing tools. Useful for competitive intelligence, sales prospecting, and technology landscape research.

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Resolves GWAS associations to candidate causal variants and credible sets via SuSiE, susie_rss, FINEMAP, CAVIAR, DAP-G, PAINTOR, PolyFun, SuSiEx, MultiSuSiE, and FOCUS. Use when narrowing a GWAS lead SNP to a 95 percent credible set, choosing between in-sample and reference LD, calibrating non-sparse loci with SuSiE-inf or FINEMAP-inf, integrating functional priors via PolyFun, fine-mapping across ancestries with SuSiEx, diagnosing LD mismatch via estimate_s_rss and kriging_rss, handling HLA or long-range LD, or feeding credible sets into coloc.susie for colocalization.

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Maps GWAS-implicated loci to candidate effector (causal) genes by integrating variant-to-gene (V2G) features via Open Targets L2G (Mountjoy 2021), MAGMA gene-based association (de Leeuw 2015), FUMA SNP2GENE, cS2G combined SNP-to-gene scores (Gazal 2022), Polygenic Priority Scores (PoPS, Weeks 2023), FLAMES, INQUISIT, DEPICT, and enhancer-gene predictors (ABC, ENCODE-rE2G). Use when narrowing a GWAS lead locus to a candidate causal gene, picking between proximity, eQTL-based, and similarity-based prioritizers, integrating multi-evidence streams (fine-mapping, colocalization, ABC enhancer-gene, distance, chromatin), reconciling discordant L2G vs PoPS calls, prioritizing tissue-specific eQTL evidence, or triangulating across at least three independent lines of evidence for a publication-grade effector-gene nomination.

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Normalize read-depth copy-ratio profiles and segment them into copy-number regions using circular binary segmentation (CBS, DNAcopy), hidden Markov models, HaarSeg, and fused-lasso methods. Covers GC-content, mappability, and replication-timing (wave-artifact) bias correction, panel-of-normals/PCA denoising, diploid-baseline centering, and algorithm selection by sequencing depth and event size. Use when choosing a segmentation algorithm, correcting depth bias, diagnosing oversegmentation or a mis-centered baseline, tuning CBS or HMM parameters, or understanding why a downstream CNV caller produced fragmented or shifted segments.

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Tests associations between categorical variables in clinical data using chi-square, Fisher's exact, Boschloo, Cochran-Mantel-Haenszel, and modern McNemar variants with calibrated confidence intervals (Wilson, Newcombe, Miettinen-Nurminen). Use when analyzing categorical outcomes, paired binary endpoints, or testing treatment-outcome independence in confirmatory or exploratory clinical trials.

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Align DNA short reads to reference genomes using bwa-mem2, the faster successor to BWA-MEM. Use when aligning DNA short reads to a reference genome.

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End-to-end biomarker discovery workflow from expression data to validated biomarker panels. Covers feature selection with Boruta/LASSO, classifier training with nested CV, and SHAP interpretation. Use when building and validating diagnostic or prognostic biomarker signatures from omics data.

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Analyzes base-editing screens for variant function. Covers library design (Sanson 2020 GRACE, Hanna 2021 BRCA1/2 SNV scanning, Cuella-Martin 2021), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end, wider for ABE8e), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

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Selects informative features for biomarker discovery using Boruta all-relevant selection, mRMR minimum redundancy, and LASSO regularization. Use when identifying biomarkers from high-dimensional omics data.

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Use when reviewing, auditing, scoring, or improving a real or synthetic MATLAB AI tutor transcript, tutoring prompt, generated lesson, exercise, feedback sequence, or skill behavior for MATLAB accuracy, active learning, assignment guardrails, feedback quality, debugging support, transfer prompts, and instructor-facing quality recommendations.

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Apply when you catch yourself writing the same instruction a second time, or notice a recurring correction. Encode the rule as a lint, metadata flag, runtime check, or script instead of more text.

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Read, transform, analyze, and generate Excel and CSV files. Use when a user asks to open a spreadsheet, process Excel data, merge CSVs, create pivot tables, clean up data, convert between Excel and CSV, add formulas, filter rows, or generate reports from tabular data. Handles .xlsx, .xls, and .csv.

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Process multiple documents in bulk with parallel execution. Use when a user asks to batch process files, convert many documents at once, run parallel file operations, bulk rename, bulk transform, or process a directory of files concurrently. Covers parallel execution, error handling, and progress tracking.

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The AWS Command Line Interface (v2) manages Amazon Web Services from the terminal. Use when the user needs to run S3, EC2, Lambda, CloudWatch, IAM or other AWS commands, sign in with SSO or short-term credentials, write AWS automation scripts, or debug an `aws` command and its output.

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When the user wants to set up authentication and authorization for a web application. Use when the user mentions "auth," "login," "OAuth," "SSO," "single sign-on," "role-based access," "RBAC," "permissions," "user roles," "access control," "authentication," or "authorization." Covers OAuth 2.0 provider integration, session management, and role/permission architecture. For JWT-specific tasks, see jwt-handler. For security review, see security-audit.

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The craft of weaving story into interactive systems where the player is both audience and author. This skill covers the unique challenges of game narrative: branching structures that respect player agency, environmental storytelling that rewards exploration, dialogue systems that feel alive, and the delicate balance between authored experience and emergent narrative. Unlike linear media, games don't tell stories TO players - they create the conditions for stories to emerge WITH players. The narrative designer's job is to build the scaffolding that supports infinite player-authored moments while maintaining coherent themes and emotional arcs. This skill synthesizes lessons from Disco Elysium's internal monologue as gameplay, Hades' death-as-narrative-engine, interactive fiction's branching mastery (Inkle, Twine), and procedural narrative pioneers like Dwarf Fortress. It addresses ludonarrative harmony, bark systems, cinematics that respect agency, and the "and therefore" test that separates plot from story. Use when "narrative design, game story, game writing, dialogue system, branching narrative, player choice, environmental storytelling, bark system, quest design, lore delivery, ludonarrative, interactive fiction, inkle, twine, dialogue tree, player agency, cinematic design, voice direction, localization, procedural narrative, narrative, game-writing, dialogue, branching, player-agency, environmental-storytelling, quest-design, voice-acting, localization, interactive-fiction, procedural-narrative, ludonarrative" mentioned.

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Git is deceptively simple to learn and incredibly hard to master. The difference between a team that ships and a team that fights merge conflicts all day comes down to workflow discipline. Your git history is documentation - make it tell a story future you can understand. This skill covers branching strategies (trunk-based, gitflow, GitHub flow), commit hygiene, merge vs rebase, conflict resolution, and the commands you actually need. Key insight: most git disasters come from not understanding what you're about to do. 2025 lesson: Trunk-based development with short-lived branches has won. Long-lived feature branches are a code smell. If a branch lives more than a few days, something is wrong with your architecture or process. Use when "git workflow, branching strategy, git merge, git rebase, merge conflict, git commit, trunk-based, gitflow, git history, git revert, git reset, git, version-control, workflow, branching, commits, merge, rebase, collaboration" mentioned.

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Expert patterns for HubSpot CRM integration including OAuth authentication, CRM objects, associations, batch operations, webhooks, and custom objects. Covers Node.js and Python SDKs. Use when "hubspot, hubspot api, hubspot crm, hubspot integration, contacts api, hubspot, crm, marketing, sales, api" mentioned.

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Go is the language of infrastructure. From Docker to Kubernetes to the entire cloud-native ecosystem, Go powers the systems that run the internet. It's not about what you can build - it's about what you won't break at 3 AM. This skill covers idiomatic Go patterns, error handling, concurrency with goroutines and channels, HTTP servers, microservice architecture, and the standard library that makes Go so powerful. Key insight: Go's simplicity is a feature. Fight the urge to abstract. Embrace boring, readable code. 2025 lesson: The teams succeeding with Go are the ones who resist overengineering. A main.go with 500 lines beats a "clean architecture" with 50 packages. Use when "golang, go service, go microservice, goroutine, channels, go http, go api, go backend, gin, fiber, chi router, go concurrency, go, golang, microservices, backend, concurrency, goroutines, channels, http, api" mentioned.

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GraphQL gives clients exactly the data they need - no more, no less. One endpoint, typed schema, introspection. But the flexibility that makes it powerful also makes it dangerous. Without proper controls, clients can craft queries that bring down your server. This skill covers schema design, resolvers, DataLoader for N+1 prevention, federation for microservices, and client integration with Apollo/urql. Key insight: GraphQL is a contract. The schema is the API documentation. Design it carefully. 2025 lesson: GraphQL isn't always the answer. For simple CRUD, REST is simpler. For high-performance public APIs, REST with caching wins. Use GraphQL when you have complex data relationships and diverse client needs. Use when "graphql, graphql schema, graphql resolver, apollo server, apollo client, graphql federation, dataloader, graphql codegen, graphql query, graphql mutation, graphql, api, apollo, schema, resolvers, dataloader, federation, typescript" mentioned.

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