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Plan a feature or initiative through a computed route: the conductor derives the canon delta and assembles the document package — from a zero-document null route for small fixes to an umbrella PRD with one spec per capability for large initiatives. Modes, named as the first word: sdd (full package), sources (MRD → BRD → URD) for market research, customer discovery, and business or user requirements, iso (BRS → StRS → SyRS → SRS) for ISO 29148 and regulated work, research for a technical investigation — never market research — that the research instrument closes either by scope coverage (a research document) or by a recommendation (an rnd). Explicit form: plan research <topic>. Use for 'plan the X redesign', 'create a roadmap', 'plan a new feature', 'I need market research before we plan', 'we're regulated — start the ISO requirements cascade', 'investigate X before we plan', 'compare the alternatives for Y' — a new investigation, not a finished report already in hand. Not for recording a decision or documenting existing code — use /archcore:document. Not for checking docs against code — use /archcore:review.

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Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3 (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

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Design, select, and analyze waveforms for radar, sonar, and active sensing using the Phased Array System Toolbox. Covers LFM, NLFM, FMCW, phase-coded, CW, stepped FM, custom IQ, ambiguity functions, sidelobe reduction, and Doppler tolerance. Key objects: phased.LinearFMWaveform, phased.NonlinearFMWaveform, phased.CustomFMWaveform, phased.PhaseCodedWaveform, phased.FMCWWaveform, phased.SteppedFMWaveform, phased.MFSKWaveform, phased.RectangularWaveform, nlfmspec2freq, shapespectrum, ambgfun, pambgfun, sidelobelevel, legendreseq, mlseq, radarWaveformGenerator.

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A platform decision framework for experimentation. When to use Statsig vs PostHog vs GrowthBook vs Optimizely vs Amplitude vs Eppo vs Kameleoon. How to migrate between them. How to coordinate when multi-platform is genuinely warranted. The decisions that compound for years and the ones you can defer. Triggers on which experimentation platform, choose Statsig vs PostHog, evaluate experimentation tools, switch experimentation platform, migrate from Optimizely, consolidate experimentation tools, multi-platform experimentation, experimentation platform decision, ab test platform selection, feature flag platform vs experiment platform, warehouse-native experiments, vendor lock-in experimentation. Also triggers when a team is asking about cost, governance, or migration cost across experimentation tools, or when an evaluation is starting.

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Creates a Check for Understanding (CFU) for a math topic or standard: 1–3 targeted formative items whose distractors come from documented student misconceptions, plus a teacher guide routing each likely response to a specific next instructional step. Load BEFORE asking any clarifying question about grade, standard, or topic. This skill is for math; for other subjects, help as you normally would and don't mention the skill to the user. Triggers on asks to create, write, generate, or design a check for understanding, formative check, quick check, or exit ticket for a math concept or standard, and on implicit signals like "I need to see who got it before I move on." Not a quiz, test, or practice set — a CFU is read for what it reveals, not scored, so a numbered quiz or a bare answer key is a different job. Does not create a lesson (k12-lesson-plan-creation, which brings its own exit ticket), tier one (k12-lesson-differentiation), or prepare a teacher to teach one (k12-lesson-prep).

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Create, modify, debug, or review Windows Forms applications only when the request contains a concrete Windows Forms marker. USE FOR: WinForms, Windows Forms, System.Windows.Forms, WinForms Form or UserControl designer files (*.Designer.cs or *.Designer.vb), Visual Studio WinForms Designer, TableLayoutPanel, BindingSource, DataGridView, Control.InvokeAsync, component-tray ownership, or custom control serialization. DO NOT USE FOR: WPF or WPF XAML, including Window.InputBindings, KeyBinding, commands, or DataContext; .NET MAUI; Avalonia; or any request where none of the listed Windows Forms markers is present.

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Conform is a React form validation library that progressively enhances native HTML forms: it validates FormData against a Zod, Valibot or Yup schema on the server and, optionally, on the client, so a form works before JavaScript loads. Use when a user asks to build a form with Conform, validate a Next.js Server Action or a React Router / Remix action, share one schema between client and server, add or remove rows in a field list, or move to Conform's future API.

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数据工程 — 数据平台从业者的认知操作系统, 覆盖把数据从源系统搬运成可靠 / 可查询 / 可信赖形态供分析 / ML / 数据产品消费的全生命周期 (生成 → 摄取 → 存储 → 转换 → 服务 + 安全/数据管理/DataOps/数据架构/编排/软件工程 六条暗流, Reis & Housley 框架): 摄取与集成 (批 + CDC 变更数据捕获 Debezium + EL 工具 Fivetran/Airbyte/Meltano/dlt + Kafka Connect + schema drift) / 存储与文件表格式 (对象存储数据湖 + 列存 Parquet/ORC/Arrow/Avro + 开放表格式 Apache Iceberg/Delta Lake/Apache Hudi + lakehouse + 分区/compaction) / 转换与建模 (ELT dbt/SQLMesh + Spark + 维度建模 Kimball + Inmon + Data Vault + 大宽表 OBT + 渐变维 SCD + 增量模型 + 语义/指标层) / 编排与工作流 (Apache Airflow/Dagster/Prefect/Mage/Kestra/Apache DolphinScheduler + DAG + 幂等 + 回填 backfill + 数据资产调度) / 批流与实时 (Apache Kafka/Apache Flink/Spark Structured Streaming/Kinesis/Pulsar/Redpanda + Lambda vs Kappa + watermark/窗口/exactly-once + 流式 SQL Materialize/RisingWave + 实时 OLAP ClickHouse/Apache Druid/Apache Pinot/StarRocks/Apache Doris) / 数仓与查询引擎 (Snowflake/BigQuery/Redshift/Databricks SQL/Trino/Presto/DuckDB/Polars + 存算分离 + MPP) / 数据质量测试与可观测性 (dbt tests/Great Expectations/Soda + 数据契约 + Monte Carlo data downtime + 新鲜度/量/schema 异常检测) / 数据治理编目与血缘 (DataHub/Amundsen/OpenMetadata/Unity Catalog + 列级血缘 + PII 分类 + 访问控制 + GDPR) / DataOps 与可靠性 (数据 CI/CD + 转换版本控制 + 环境隔离 + 幂等重处理 + 数据 SLA/SLO + 计算存储 FinOps) / 数据架构范式 (现代数据栈 + lakehouse + data mesh + data fabric + 去中心化 vs 中心化所有权) / 分析工程角色 (dbt 时代连接数据工程与分析的桥) — 不含 数据科学/ML 建模本身 (是下游消费者) / BI 仪表盘制作 (serving 下游) / 数据分析报表为终点 / 'data engineer = 跑 Hadoop 的' 过时窄化 / 通用后端应用开发 (平行学科) (Data Engineering — the cognitive operating system of practitioners who design, build, and operate the data platform: moving data from source systems into reliable, queryable, trustworthy form for analytics / ML / products, covering (a) the data engineering lifecycle (generation → ingestion → storage → transformation → serving, with the undercurrents security / data management / DataOps / data architecture / orchestration / software engineering — Reis & Housley framing), (b) ingestion & integration (batch + CDC change-data-capture with Debezium, EL tools Fivetran / Airbyte / Meltano / dlt, Kafka Connect, API + file + database sources, schema drift handling), (c) storage & file/table formats (object storage data lakes, columnar formats Parquet / ORC / Arrow / Avro, open table formats Apache Iceberg / Delta Lake / Apache Hudi, lakehouse architecture, partitioning / compaction / Z-ordering), (d) transformation & modeling (ELT with dbt / SQLMesh, Spark, dimensional modeling Kimball, Inmon CIF, Data Vault, One Big Table / wide tables, normalization vs denormalization, slowly changing dimensions, incremental models, the semantic / metrics layer), (e) orchestration & workflow (Apache Airflow, Dagster, Prefect, Mage, Kestra, Apache DolphinScheduler, DAGs, idempotency, backfills, data-aware / asset-based scheduling), (f) batch vs streaming & real-time (Apache Kafka, Apache Flink, Spark Structured Streaming, Kinesis / Pulsar / Redpanda, the Lambda vs Kappa debate, watermarks / windowing / exactly-once, streaming SQL Materialize / RisingWave, real-time OLAP ClickHouse / Apache Druid / Apache Pinot / StarRocks / Apache Doris), (g) warehouses & query engines (Snowflake, BigQuery, Redshift, Databricks SQL, Trino / Presto, DuckDB, Polars, decoupled storage & compute, MPP), (h) data quality, testing & observability (dbt tests, Great Expectations, Soda, data contracts, Monte Carlo / data downtime, freshness / volume / schema anomaly detection, unit / integration testing of pipelines), (i) data governance, catalog & lineage (DataHub, Amundsen, OpenMetadata, Unity Catalog, column-level lineage, PII / data classification, access control, GDPR / data privacy), (j) DataOps & reliability (CI/CD for data, version control of transformations, environments, idempotent reprocessing, SLAs / SLOs for data, cost / FinOps for compute & storage), (k) data architecture paradigms (modern data stack, data lakehouse, data mesh, data fabric, decentralized vs centralized ownership), (l) the analytics engineering role (the dbt-era bridge between data engineering and analysis); N

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GitHub 解封 — 账号封禁/限制/恢复的领域操作系统。覆盖：(a) GitHub 官方政策体系 (TOS / 可接受使用政策 / Trust & Safety 执行模式 / 公开申诉路径 / 受制裁地区解读)；(b) 封号原因诊断学 (spam / abuse / 制裁误标 / 2FA 丢失 / ToS 违规 / 账号劫持误判 / ban evasion 等子类型 + 症状→原因映射 + 自我分诊压测)；(c) 申诉实操 craft (写一封能让 T&S 快速判误伤的英文申诉信 + 升级阶梯 + 法务介入边界 + Don'ts)；(d) 制裁与合规硬边界 (OFAC SDN 名单 / 哪些国家或地区真不可达 / VPN 误判路径 / 中国大陆不是美国制裁地区的反复重申)；(e) 中国大陆开发者特别处境 (sanctioned region 误标 + SMS 验证不支持 +86/+852 + Gitee/GitLab/Codeberg/JihuLab 备用迁移 + 私有自托管 Gitea/Forgejo)；(f) 真实案例库 (~30+ 公开案例分类拆解：误伤 / 真违规 / 制裁误标三类各自赔率与申诉成功路径)。伦理锚绝不软化：不教 ban evasion / 不教撒谎式申诉 / 真违规承认改进 / 丢 2FA 无恢复因子则诚实告知 / 中国制裁误标靠申诉不靠换 VPN / 申诉 6 个月窗口必算清 / 约 3% (业内估) 真违规恢复 vs 约 79% (业内估) 误伤申诉成功率分层标。 (GitHub Account Reinstatement — the domain operating system for GitHub (and analogous platform) account suspension, restriction, appeal, and recovery. Covers: (a) GitHub official policy architecture (TOS / Acceptable Use Policies / Trust & Safety enforcement patterns / public appeals pathways / sanctioned-region interpretation); (b) suspension-cause diagnostics (spam / abuse / sanctions mis-flag / 2FA loss / ToS violation / account hijack false-positive / ban evasion taxonomy + symptom→cause mapping + self-triage pressure test); (c) appeal craft (writing an English appeal letter that gets T&S to fast-track a false-positive ruling + escalation ladder + legal-engagement boundary + Don'ts); (d) sanctions and compliance hard boundaries (OFAC SDN list / which countries/regions are truly unreachable / VPN false-positive paths / China is NOT a US-sanctioned region — repeated emphasis); (e) mainland China developer special circumstances (sanctioned-region mis-flag + SMS verification does not support +86/+852 + Gitee/GitLab/Codeberg/JihuLab fallback migration + self-hosted Gitea/Forgejo); (f) real-case library (~30+ public cases with categorized analysis: false-positive / genuine violation / sanctions mis-flag, each with win-rate and appeal success path). Ethical anchors absolutely not softened: do NOT teach ban evasion (creating new accounts turns a temp ban into a permanent one — the single biggest mistake); do NOT teach social engineering or lying in appeals (misuse + actually confirms violation); genuine violations: admit + improve beats denial; lost 2FA + no recovery factor → do not pretend recovery is possible; China sanctioned-region mis-flag → fix via appeal, not repeated VPN switching; appeal form 6-month window must be counted carefully; historical 约 3% (业内估) genuine-violation recovery vs 约 79% (业内估) false-positive appeal success rate stratification must be honestly labeled. Anti-samples (explicitly describe harm, never endorse): developer permabanned for ban evasion / open-source team org-banned for personal attacks in public issues / cases where repeated VPN switching escalated risk-control severity.) Master OS — automated mastery of GitHub Account Reinstatement — the domain operating system for GitHub (and analogous platform) account suspension, restriction, appeal, and recovery. Covers: (a) GitHub official policy architecture (TOS / Acceptable Use Policies / Trust & Safety enforcement patterns / public appeals pathways / sanctioned-region interpretation); (b) suspension-cause diagnostics (spam / abuse / sanctions mis-flag / 2FA loss / ToS violation / account hijack false-positive / ban evasion taxonomy + symptom→cause mapping + self-triage pressure test); (c) appeal craft (writing an English appeal letter that gets T&S to fast-track a false-positive ruling + escalation ladder + legal-engagement boundary + Don'ts); (d) sanctions and compliance hard boundaries (OFAC SDN list / which countries/regions are truly unreachable / VPN false-positive paths / China is NOT a US-sanctioned region — repeated emphasis); (e) mainland China developer special circumstances (sanctioned-region mis-flag + SMS verification does not support +86/+852 + Gitee/GitLab/Codeberg/JihuLab fallback migration + self-hosted Gitea/Forgejo); (f) real-case library (~30+ public cases with categorized analysis: fa

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Make an original animated music video (MV / PV) for a song from its audio file plus lyrics, in the Vocaloid / J-pop lyric-video tradition: 手書き/tegaki (V家手书, 手描きMV, ボカロPV), 文字PV and kinetic typography (リリックモーション, リリックビデオ, 歌詞動画, 歌词视频, 动态歌词), anime-illustration MVs with an original character — for any mood, from dark ballads to bright upbeat summer pop. Renders to MP4 with a deterministic Canvas2D/WebGL2 engine in a headless browser: JIZURA lyric motion, AI-generated art, optional Blender toon 3D, beat-locked editing, 9:16 vertical/Shorts version. Use it whenever the user wants a music video, MV, PV, lyric video or beat-synced animation for a song, e.g. "make an MV for my song", "turn this track into a lyric video", "给这首歌做个MV/PV", "做个V家手书", "この曲のMVを作って", "ボカロPV作って", "縦型ショートも" — even if they only give a .wav/.mp3 and say "make a cool video for it". Not for editing existing footage or clip compilations.

Analyze and optimize X (Twitter) posts for viral potential and reach using heuristics inspired by X's published open-source recommendation architecture. Use when user wants to: (1) Check if a post will go viral, (2) Score a tweet for engagement potential, (3) Optimize or rewrite a tweet for algorithmic reach, (4) Understand why a tweet underperformed, (5) Build audience in a specific niche. Triggers: "Check if this will go viral", "Make this post buzz", "Will this tweet perform well?", "Optimize my tweet", "How can I make this viral?", "rewrite this tweet", "improve my tweet engagement", "twitter algorithm", "tweet reach", "debug underperforming tweet", "バズるかチェックして", "Xでバズる投稿にして", "伸びるかチェックして", "この投稿を伸ばして", "投稿を改善して", "ツイートを最適化して"

Tested output format specifications for Claude prompts. Use when the user wants a specific deliverable structure — retrieving, reviewing, customizing, or building output formatting. Trigger on: "give me the Executive Brief format," "show me the output template for," "I need the Decision Matrix structure," "show me available output formats," "build a custom output format for." Provides 10 ready-to-use structures (executive briefs, technical designs, research summaries, implementation plans, decision matrices, competitive analyses, post-mortems, stakeholder updates, strategic memos, process documentation) plus a custom template, with per-section length guidance and format constraints tested against Claude formatting defaults. If unsure which format fits, use rootnode-block-selection first if available. Do NOT use for evaluating existing prompts (use rootnode-prompt-validation if available) or choosing reasoning/identity approaches (use rootnode-block-selection if available).

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State management decision tree for Vue 3 SPAs: ref/reactive locally, composables for shared logic, Pinia for app-wide state, provide/inject for DI, TanStack Query Vue for server state. Vuex pointer for Vue 2 legacy. Use this skill to: - Pick the right state tool for the data shape (local / shared / server / form). - Implement a Pinia store (Setup syntax preferred). - Use provide/inject correctly with InjectionKey<T>. - Set up TanStack Query Vue for server state. - Migrate from Vuex (with caution; only if BA asks). Do NOT use this skill for: - General SFC conventions (see vue-conventions). - Form state (see vue-forms — vee-validate handles that). - Routing state (see vue-routing — use vue-router useRoute). - Testing stores (see vue-testing).

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Full-funnel paid advertising operations for B2B SaaS. Use this skill for PPC strategy, Google Ads optimization, LinkedIn Ads, social media advertising, programmatic buying, creative strategy, attribution modeling, budget allocation, budget pacing and planned-vs-delivered reconciliation, audience overlap and campaign self-competition audits, suppression lists, ROAS improvement, media spend optimization, and the media no auction sells — newsletter and podcast sponsorships, community and industry-publication placements, paid review-site listings on G2 and Capterra, and pay-per-lead content syndication. It also covers buying on the AI answer surface — ads inside Google AI Overviews and AI Mode that existing Search, Shopping and Performance Max campaigns already serve into with no opt-out, no placement targeting and no segmented reporting, and ChatGPT Ads and Microsoft Copilot as separate opt-in platforms with their own plan-tier reach limits, no negative keywords, and a landing-page crawler gate. It also owns the conversion signal the bidding algorithms learn from — CRM pipeline stages (SQL, opportunity, closed-won) sent back to Google Ads, LinkedIn, Meta and Microsoft Ads through offline conversion import, enhanced conversions for leads, the Google Data Manager API and the LinkedIn Conversions API, with click-ID capture, stage values, dedupe, EEA consent signals and retractions. It also gates connected TV (CTV) and streaming video for B2B — whether a reach medium belongs in the plan, how a vendor ties a TV household to a target account (member data, IP-to-account graph or contextual), the server-side ad insertion (SSAI) fraud questions, Programmatic Guaranteed deals, and a holdout lift test because CTV has no click. It also runs person-led paid social — LinkedIn Thought Leader Ads and Meta partnership ads that put spend behind an employee's or creator's post — on a paid-usage term, an in-post disclosure check and an end-date register, because the platform approval carries no term. Also triggers on: CTV, connected TV, OTT, streaming TV ads, should we run CTV, LinkedIn CTV, B2B CTV, account-based CTV, SSAI fraud, thought leader ads, boost an employee's post, sponsor a creator's post, partnership ads, whitelisting, creator usage rights, PPC, Google Ads, LinkedIn Ads, social ads, programmatic, ad creative, attribution, media budget, paid campaigns, ROAS, CPA, ad spend, newsletter sponsorship, podcast sponsorship, community sponsorship, sponsor a newsletter, content syndication, pay-per-lead, cost per lead vendor, media kit, rate card, insertion order, direct buy, publisher partnership, make-good, G2 paid listing, Capterra ads, review site advertising, sponsorship ROI, budget pacing, we underspent our budget, are our campaigns competing with each other, audience overlap, self-competition, campaign cannibalization, suppression list, exclusion list, ad frequency across channels, how many ad accounts do we have, ads in AI Overviews, AI Mode ads, ChatGPT Ads, OpenAI Ads Manager, Copilot ads, conversational ads, should we advertise on ChatGPT, can we buy our way into the AI answer, our ads are showing in AI Overviews and we cannot report on it, context hints, OAI-AdsBot, our ad landing page fails review, robots.txt is blocking our ads, our paid leads are junk, Google Ads is optimizing for the wrong leads, offline conversion import, OCI, enhanced conversions for leads, send CRM stages back to Google Ads, upload SQLs to LinkedIn, LinkedIn Conversions API, LinkedIn CAPI, Meta conversion leads, CRM to ad platform, gclid, li_fat_id, msclkid, value-based bidding, optimize for pipeline not form fills, low match rate, conversion lag.

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Use when designing or reasoning about an Agentforce Grid worksheet — the spreadsheet-like Agentforce workspace where rows are jobs and columns are sequential Data / AI / Action steps that process left to right — to run bulk record updates, generate insights, or test multi-turn AI conversations over CRM or Data Cloud (Data 360) data without Apex or Flow. Trigger keywords: Agentforce Grid, workbook, worksheet, grid column, data column, AI column, action column, bulk AI update, Flex Credits per row, metered compute. NOT for building the agent, its topics, or its actions themselves (use agentforce/* action and topic skills), NOT for single-record prompt design (use the prompt-builder skills), NOT for deployable metadata/Apex (Grid is a no-code Studio tool, not a metadata type), and NOT for AI governance/ethics policy (use admin/ai-ethics-and-governance-requirements).

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Use when deploying an Agentforce agent to a channel surface: Embedded Service web chat, Slack, Experience Cloud, Agent REST API, or Salesforce Mobile. Triggers: 'deploy agent to website', 'add Agentforce to Slack', 'enable agent for mobile app', 'configure embedded service for agent', 'agent API for custom integration', 'multi-channel agent deployment'. NOT for agent logic design — use agentforce/agent-topic-design or agentforce/agent-actions for topics, instructions, and action contracts.

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Authoring and managing Agentforce agent definitions using the declarative Agent Script DSL (.agent files) and associated metadata types, including the Agent Script language for the new Agentforce Builder (hybrid-reasoning -> logic vs | prompt instructions, @actions/@subagent/@variables references, subagents). Use when creating agents in source control, writing or debugging Agent Script, debugging agent metadata, or understanding the metadata lifecycle of GenAiPlugin/GenAiPlanner/BotVersion types. Triggers: 'how do I deploy an Agentforce agent using source control', 'convert an agent topic to a subagent', 'what metadata types make up an Agentforce agent', 'agent test run command failing in CI pipeline', 'GenAiPlugin vs GenAiPlanner metadata relationship', 'Agent Script logic vs prompt instructions'. NOT for Apex-based agent actions — use agentforce/custom-agent-actions-apex. NOT for clicking the agent together in the Builder UI — use agentforce/agentforce-agent-creation.

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Identify, score, and prioritize Salesforce AI use cases before any implementation begins — opportunity identification, Impact-Effort matrix scoring, feasibility evaluation across Technical/Operational/Data/Risk dimensions, and ROI framing. NOT for the license checks and enablement steps once a use case is approved — use agentforce/agentforce-service-ai-setup or agentforce/agentforce-sales-ai-setup. NOT for making the data model AI-ready — use architect/ai-ready-data-architecture.

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Route ordinary public-web information tasks to the correct Tavily CLI operation. Use when the task concerns publicly accessible webpages and needs web discovery, clean extraction, URL mapping, same-site crawling, or public-web-only research, but the exact Tavily operation is not yet clear or several operations must be sequenced. Prefer the specific leaf skill when intent is clear: tavily-search for discovery without a URL, tavily-extract for known URLs, tavily-map for URL/site-structure discovery, tavily-crawl for content from many pages on one site, tavily-research for cited multi-source synthesis, and tavily-dynamic-search for programmatic filtering with context isolation. Do not use for named social/community/media/career/code platforms, login or cookie-dependent content, platform-native discussions, or cross-platform collection; use Agent Reach there. Do not use for local files, Git operations, deployments, or ordinary code editing.

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Serverless form handling on Netlify-hosted sites — detects HTML forms at deploy time, stores submissions, filters spam, and sends notifications. Use when adding a contact form, lead-capture form, file-upload form, or newsletter signup to a Netlify site; wiring AJAX form submission; setting up a custom thank-you page; adding a honeypot or reCAPTCHA to a form; getting forms working in Next.js, Nuxt, SvelteKit, Astro, or Gatsby; reading form submissions via the Netlify API; or debugging missing submissions and forms that silently fail to register.

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Write or edit Japanese articles, reports, guides, and public-facing drafts so the argument is clear and the prose reads naturally. Preserve the author's meaning, evidence, and voice when revising. Use for requests to write from a topic or notes, remove AI臭 / AIっぽさ, improve awkward wording or structure, or format notes as 箇条書き, チーム共有, 社内共有, a decision note, handoff, Slack post, or Teams post. For existing prose, ask Gemini through Antigravity CLI to rewrite naturally by default, then audit against the source; use the current model if unavailable. Do NOT use for ordinary Japanese answers, progress updates, technical explanations, code review, English-only prose that is not a bullet-formatting request, or an explicitly incompatible format.

Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.

日本語の概要は準備中です。原文の説明を表示しています。

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