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Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.

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Generate alignment statistics using samtools flagstat, stats, depth, coverage, and mosdepth. Use when assessing alignment quality, calculating coverage, or generating QC reports.

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Access Core Motion accelerometer, gyroscope, magnetometer, device-motion, pedometer, activity-recognition, altitude, headphone motion, batched high-frequency workout motion, and water-submersion/depth data. Use when reading device sensors, counting steps, detecting walking/running/driving/cycling, tracking altitude, building motion interactions, handling AirPods head tracking, or implementing watchOS dive/depth features.

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Trace error propagation through a multi-agent session by agent depth — where failures originated, the depth at which they appeared, and how they cascaded up to parent agents — using the Agent Monitor workflow intelligence API and the session event stream. Use when a multi-agent run failed and you need to find the origin and blast radius of the failure.

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Debug a specific session by inspecting its full event chain (PreToolUse, PostToolUse, Stop, SubagentStop, Compaction, APIError, TurnDuration, Notification events), agent hierarchy (recursive parent/child tree with subagent_type and depth), token usage with compaction baselines, workflow intelligence data (orchestration DAG, error propagation by depth), and session metadata (thinking_blocks, turn_count, total_turn_duration_ms).

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Progressive memory recall with 4 scope layers AND 3 depth layers. Scope: identity > project > room > deep. Depth: IDs only > summary > full. 10-50x token savings through fetch-on-confirmation pattern.

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Use when improving internal link structure, anchor text, orphan pages, crawl depth, site architecture, or link equity flow. 内链优化/站内架构

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Comprehensive best practices for robot perception systems covering cameras, LiDARs, depth sensors, IMUs, and multi-sensor setups. Use this skill when working with RGB image processing, depth maps, point clouds, sensor calibration (intrinsic, extrinsic, hand-eye), object detection, semantic segmentation, 3D reconstruction, visual servoing, or perception pipeline optimization. Trigger whenever the user mentions OpenCV, Open3D, PCL, RealSense, ZED, OAK-D, camera calibration, AprilTags, ArUco markers, stereo vision, RGBD, point cloud filtering, ICP registration, coordinate transforms, camera intrinsics, distortion correction, image undistortion, sensor streaming, frame synchronization, or any computer vision task in a robotics context. Also covers multi-camera rigs, time synchronization across sensors, perception latency budgets, and production deployment of perception pipelines.

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Investigate how a concept, code path, application, network flow, system, incident, document, or local artifact actually works, then deliver a two-depth visual HTML explainer with a dead-simple big-picture first layer and source-backed technical depth behind it. Use when the user invokes $xray, asks how something works under the hood, wants a module or app behavior traced across boundaries, requests web, code, network, or safe static artifact research before explanation, or asks for an evidence-backed explainer. Do not use for answer-only definitions, general web design, full codebase audits, or invasive binary reverse engineering without explicit authorization.

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Think and work like an expert Atomic, Molecular & Optical Physicist. Use when a task calls for Atomic, Molecular & Optical Physicist judgment. Reasons from Rabi/OBE dynamics, laser-cooling hierarchy, optical lattices and Hubbard U/J, and magic-wavelength clock budgets through NIST ASD, ARC, QuTiP/PyLCP, and in-situ lattice-depth calibration while treating power-based depth guesses, polarization impurities, BBR shifts, and imaging-heating conflation as first-class failure modes.

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Detect somatic and germline copy number variants from targeted, exome, and whole-genome sequencing with CNVkit, a read-depth caller that combines on-target and off-target (antitarget) coverage. Covers panel-of-normals construction, flat-reference tumor-only calling, hybrid/amplicon/WGS modes, CBS vs HMM segmentation selection, purity-aware integer calling, and reconciliation against GATK and allele-specific callers. Use when calling CNVs from hybrid-capture panels or exomes, deciding whether CNVkit (depth-only) is the right tool versus an allele-specific caller, building a panel of normals, diagnosing flat-reference false positives, or interpreting log2 ratios into copy-number states.

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Generate alignment statistics using samtools flagstat, stats, depth, coverage, and mosdepth. Use when assessing alignment quality, calculating coverage, or generating QC reports.

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Validate that analytics and marketing tracking fire CORRECTLY: GA4/GTM dataLayer events, Meta/TikTok/LinkedIn pixels, and ad-tech tags. Covers building a tracking plan as the contract, intercepting collect-endpoint beacons and dataLayer.push in Playwright, asserting event name + params + values + timing + de-duplication, Consent Mode v2 gating, CI regression gating, and news-media events (article-view, scroll-depth, paywall). Use when: "test analytics tracking," "GA4 event test," "verify the pixel fires," "dataLayer test," "tracking plan," "Meta Pixel dedup," "scroll-depth tracking test," "gate tracking in CI." Not for: whether tracking is ALLOWED to fire under consent law (GDPR/CMP) — that is compliance-testing; this skill checks the data is CORRECT. SEO meta tags / structured data — out of scope. Related: compliance-testing, playwright-automation, api-testing, qa-project-context.

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Read live Hyperliquid market data from the desk computer with curl or the Python SDK - mid, mark and oracle prices, order book depth, funding (current, predicted, historical), open interest, volume, candles, perp and spot metadata, margin tiers, and how to save datasets for the strategy lab. Read-only, no key. Use for any market brief, depth read, funding question or data pull.

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Set up Blender cameras with cinematic intent — focal length, depth of field (f-stop / focus object), composition (rule of thirds, leading lines), animated cameras (orbit, dolly, push-in), tracking constraints. Use whenever the user asks to "set up the camera", "frame the shot", "make it look cinematic / hero / portrait / wide-angle / telephoto", "add depth of field", "orbit the camera", or any composition/framing request. Make sure to use this skill even if the user does not say "camera" — also covers "hero shot", "close-up", "from above", "shallow focus", "85mm portrait look".

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Orchestrates the dedicated Kinect wall harp implementation team. Use whenever the user asks to build, implement, continue, fix, QA, document, or ship the Kinect wall harp, Kinect v2 wall-depth harp, FreenectTD depth-blob hand tracking, projected wall strings, pluck synth harp, or this feature's Layer 1 tool/recipe/docs. Use this before the generic tdmcp-pipeline for this feature.

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Eight tested identity approaches for Claude prompts, each shaping depth, vocabulary, reasoning style, and what Claude treats as obvious vs. requiring explanation. Use when the user wants a specific identity template — retrieving, reviewing, customizing, or building a role definition. Trigger on: "give me the Strategic Advisor identity," "show me the identity template for," "I need a Technical Architect role," "build a custom role for," "show me all available identities." Also use when reviewing a prompt's identity layer or when output lacks domain-appropriate depth. Provides 8 tested approaches across strategy, technical, research, communications, and operations domains plus a template for building custom identities. If the user is unsure which identity fits their task, use rootnode-block-selection first if available. Do NOT use for evaluating complete prompts — use rootnode-prompt-validation if available. Do NOT use for full prompt assembly — use rootnode-prompt-compilation if available.

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Exploit Active Directory environments using Pass-the-Hash (PtH). This skill details how to authenticate to remote systems using the NTLM hash of a user's password instead of the plaintext password, enabling lateral movement and privilege escalation without cracking hashes.

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Calculates statistical power for high-dimensional genomics experiments (bulk RNA-seq, scRNA-seq, ATAC-seq, ChIP-seq, methylation, proteomics) under negative-binomial count models using RNASeqPower, PROPER, and simulation via powsimR, distinguishing per-gene from marginal (transcriptome-wide) power, the role of mean expression and dispersion, and the sequencing-depth-versus-replicate tradeoff. Covers simulation as the honest default for overdispersed counts, FDR-aware average power versus single-test power, observed/post-hoc power as an anti-pattern, and the winner's-curse / Type-S / Type-M consequences of underpowering. Use when planning replicate number for a sequencing experiment, deciding whether to add depth or samples, choosing closed-form versus simulation power, estimating power from pilot dispersions, or justifying replication in a grant. For clinical-trial power see clinical-biostatistics/power-and-sample-size; for the inverse sample-size question see experimental-design/sample-size.

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Estimates the minimum biological replicates (or cells/events) for a target power at a target FDR in genomics experiments using ssizeRNA, PROPER, powsimR for scRNA-seq, and pilot-data dispersion estimation from DESeq2/edgeR. Covers the biological-versus-technical replication distinction (technical replicates do not add degrees of freedom for biological inference), replicate-number-versus-sequencing-depth budgeting, scRNA-seq sample-versus-cell allocation under a pseudobulk model, and the critique that "n=3" is a publication convention rather than a power calculation. Use when budgeting a sequencing experiment, writing the sample-size justification in a grant, estimating replicates from pilot data, allocating a fixed budget between samples and depth, or planning scRNA-seq cohort size. For clinical-trial sample size see clinical-biostatistics/power-and-sample-size; for the power-given-n direction see experimental-design/power-analysis.

日本語の概要は準備中です。原文の説明を表示しています。

Detect somatic and germline copy number variants from targeted, exome, and whole-genome sequencing with CNVkit, a read-depth caller that combines on-target and off-target (antitarget) coverage. Covers panel-of-normals construction, flat-reference tumor-only calling, hybrid/amplicon/WGS modes, CBS vs HMM segmentation selection, purity-aware integer calling, and reconciliation against GATK and allele-specific callers. Use when calling CNVs from hybrid-capture panels or exomes, deciding whether CNVkit (depth-only) is the right tool versus an allele-specific caller, building a panel of normals, diagnosing flat-reference false positives, or interpreting log2 ratios into copy-number states.

日本語の概要は準備中です。原文の説明を表示しています。

Generate alignment statistics using samtools flagstat, stats, depth, coverage, and mosdepth. Use when assessing alignment quality, calculating coverage, or generating QC reports.

日本語の概要は準備中です。原文の説明を表示しています。

Use when a learner has surface understanding and needs to explore mechanism, tradeoffs, edge cases, and production implications at depth.

日本語の概要は準備中です。原文の説明を表示しています。

Knowledge base from the Expanded Guidance for NASA Systems Engineering (NASA/SP-2016-6105-SUPPL, Volume 1, March 2016) — the practitioner-depth supplement to the NASA SE Handbook. Use for the load-bearing mechanics the handbook compresses: the SE Engine's per-phase cadence and the iterative-vs-recursive distinction, the AS9100 crosswalk, life-cycle tailoring vs. customization and the Compliance Matrix, the recursive system-design consistency loop (ConOps vs. Operations Concept, requirement flow/type/ownership, successive refinement), product realization (verify/qualify/accept/certify cardinality, protoflight, 'test the way we fly' coverage, two-form transition), and the crosscutting technical-management procedures (six-step scheduling, cost-estimating method selection, JCL, interface document family, RIDM/CRM, CM baselines, EVM arithmetic, MOE/MOP/KPP/TPM, the three required leading indicators, and the decision-analysis method catalog). This is the DEPTH layer — it deliberately does NOT restate the base definitions; pair it with the nasa-se-handbook pack. Scope: NASA space-flight SE practice (Vol 1 Practices); thin on the Vol 2 process-appendix detail, on non-NASA/commercial life cycles, and on software-engineering methodology.

日本語の概要は準備中です。原文の説明を表示しています。