「single」の検索結果

Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

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Normalizes and transforms RNA-seq count matrices for DE, visualization, clustering, and ML. Covers between-sample (TMM, TMMwsp, RLE/median-of-ratios, upper quartile), within-sample (TPM, FPKM/RPKM), variance-stabilizing (VST, rlog, log-CPM), GC-content correction (cqn, EDASeq), and single-cell (scran deconvolution, scanpy normalize_total). Encodes the composition-bias rationale, the "most genes not DE" assumption and its catastrophic failure modes (MYC amplification, apoptosis, viral host shutoff, prokaryotic stress), the "lengthScaledTPM is not TPM" naming trap, the "TPM is not for DE" rule, the blind=TRUE vs FALSE decision, ERCC spike-in normalization (SBN), and the single-cell zero-inflation breakdown of TMM/RLE. Use when choosing or applying normalization, debugging shifted-MA-plot diagnostics, handling zero-heavy single-cell data, or correcting GC bias.

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Stores and operates on sparse expression matrices for single-cell and large bulk RNA-seq, covering dgCMatrix/dgRMatrix/dgTMatrix when-each-is-fast, the dgCMatrix (CSC, R) <-> CSR (Python) implicit transpose, AnnData (cells-rows) <-> SingleCellExperiment (cells-cols) orientation flip, HDF5/h5ad vs Zarr cloud-native shift, HDF5SummarizedExperiment + DelayedArray for out-of-memory bulk, scanpy backed mode for large h5ad, the ~10-15% density crossover where dense beats sparse, 10X format proliferation (MTX vs CellRanger H5 vs h5ad), the dense-conversion memory blow-up, and Dask + Zarr for consortium-scale matrices. Use when choosing sparse format, working with single-cell-sized matrices, importing/exporting 10X, debugging R/Python interop transposes, processing matrices too large for RAM, or building cloud-native pipelines.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

日本語の概要は準備中です。原文の説明を表示しています。

Deep single-page SEO analysis covering on-page elements, content quality, technical meta tags, schema, images, and performance. Use when user says "analyze this page", "check page SEO", "single URL", "check this page", "page analysis", or provides a single URL for review.

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Gemini CLIで質問への回答や文章生成、ファイル内容の要約を単発で実行します。モデルやJSON出力の指定、拡張機能などの管理コマンドも扱います。

• メモファイルを要約したいとき
• 単発の依頼で文章を生成したいとき
• 回答をJSON形式で受け取りたいとき

Regulator-grade feature attribution for any LSTM/Transformer signal — single-entry PageRank ranks the top-K features that drove the prediction (ADR-126 Phase 6, ADR-123 single-entry PR)

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Deliver a runnable local browser app so the user can operate it on the first handoff. This delivery skill does not choose whether a requested local system should be a browser app. On a current turn that explicitly says to implement a brand-new browser project now, load bundled:greenfield-project-scaffolding before project work only after no-peek or permitted top-level placement facts prove that no named or suitable current-folder target resolves the root; never hand off from planning or answer-only turns, existing work, a named or suitable target, or standalone or single-file delivery. Resolve user-blocking product or interface choices with an available interaction surface; resolving those choices alone never authorizes implementation. Always call read_skill for bundled:browser-app-delivery before creating or materially changing a local browser app that owns its start path or a playable browser game. Also load it when the user requests a paste-ready, single-file, offline, or immediately playable browser artifact, even if no local start path should remain, unless the user says they will open or check it themselves or explicitly declines verification. On completed local-server delivery, include exact start commands, a concrete local URL, and explicit browser-open wording. On completed standalone delivery, hand off the exact artifact path or paste URL and smoke result without inventing a server. Do not load it for a self-contained static file that has no owned start path if the user either says they will open or check it themselves or explicitly declines verification. Do not load it for component/style-only edits, deployed sites, backend/API-only work, browser QA, review, explanation, plan-only, or explicit stop/no-tool turns.

日本語の概要は準備中です。原文の説明を表示しています。

Performs Scanpy single-cell RNA-seq QC, normalization, HVG selection, PCA/UMAP/t-SNE, clustering, exploratory marker ranking, pseudobulk preparation, visualization, and Seurat or SingleCellExperiment RDS conversion to h5ad. Applies to established exploratory scRNA-seq workflows with explicit count and expression provenance; complementary skills cover scvi-tools models and AnnData format details.

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Queries the CZ CELLxGENE Census programmatically for versioned public single-cell and spatial transcriptomics data. Use when you need population-scale cell metadata, gene expression slices, Census summary counts, source H5AD URIs/downloads, embeddings, spatial Census data, or reference atlas comparisons across organisms, tissues, diseases, assays, and cell types. For analyzing your own local single-cell data use scanpy, anndata, or scvi-tools.

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Generate a concise 4-5 page equity research earnings preview for a single company. Analyzes the most recent earnings transcript, competitor landscape, valuation, and recent news to produce a professional HTML report.

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Write a publication-grade 8-part deep-dive series on a single company (~120k words total): cognitive reset / moat / profit engine / hidden assets / era variable (e.g. AI) / financials Buffett-style / management / valuation+redlines. The core IP is NOT writing but REVISING — a strict fact-check checklist catches pseudo-precision (probability-weighted expectations, third-party MAU discrepancies, linear extrapolation), absolute language, and cross-article number inconsistencies that most finance long-forms violate. Each piece stands alone but shares one valuation/management/price framework. Use when the user wants textbook-level depth on one company for public publishing (a single research report or earnings note is NOT this — use investment-research / earnings-review instead).

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Use this skill to build a multi-element Excel dashboard — Dashboard sheet on open, multiple formula-driven KPI cards, multiple charts, sparklines, and conditional formatting — from CSV or tabular input. Trigger on: 'dashboard', 'KPI dashboard', 'analytics dashboard', 'executive dashboard', 'metrics dashboard', 'CSV to dashboard', 'data visualization'. Output is a single .xlsx. Scene-layer on officecli-xlsx: inherits every xlsx hard rule. DO NOT invoke for: a single budget tracker / one-sheet CSV-with-formatting (use xlsx), a 3-statement / DCF / LBO financial model (use financial-model), a weekly report with at most 1 chart and fewer than 10 rows (use xlsx).

日本語の概要は準備中です。原文の説明を表示しています。

Deep learning for single-cell analysis using scvi-tools. This skill should be used when users need (1) data integration and batch correction with scVI/scANVI, (2) ATAC-seq analysis with PeakVI, (3) CITE-seq multi-modal analysis with totalVI, (4) multiome RNA+ATAC analysis with MultiVI, (5) spatial transcriptomics deconvolution with DestVI, (6) label transfer and reference mapping with scANVI/scArches, (7) RNA velocity with veloVI, or (8) any deep learning-based single-cell method. Triggers include mentions of scVI, scANVI, totalVI, PeakVI, MultiVI, DestVI, veloVI, sysVI, scArches, variational autoencoder, VAE, batch correction, data integration, multi-modal, CITE-seq, multiome, reference mapping, latent space.

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Converts a markdown deck (slides separated by `---` HR boundaries or by `# ` H1 headings, with optional `<!-- notes: ... -->` presenter notes blocks) into a single-file HTML presentation with arrow-key / space / PgDn / PgUp / Home / End / P / Esc keyboard navigation, presenter mode (split view with current slide + speaker notes + clock + next-slide preview), URL-hash deep linking, and `@media print` page-per-slide for PDF export. Triggers when the markdown-html-orchestrator classifies an input as SLIDES, or when invoked directly via /cs:md-slides. Reuses md-document's markdown parser for slide-body rendering and reads design-system tokens via config_loader.py. Refuses if input has no clear slide boundaries, produces a 1-slide deck, or `--strict-notes` is on with < 50% notes coverage. Use after orchestrator routing.

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Converts a markdown PR writeup or code review (one with ```diff fenced blocks and severity-tagged > [!BLOCKER]/[!MAJOR]/[!MINOR]/[!NIT] callouts) into a single-file 2-column HTML review — unified-diff on the left, severity-tagged annotation cards on the right, top jump-nav listing every finding, mandatory named reviewer footer. Triggers when the markdown-html-orchestrator classifies an input as REVIEW, or when invoked directly via /cs:md-review. Refuses without explicit --reviewer (a code review must name a human), refuses if no diff hunks present (route to md-document instead), and refuses to encode severity in color only (every badge ships color + icon + aria-label per WCAG 1.4.1). Use after orchestrator routing.

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Deploy a production self-hosted n8n end-to-end to a fresh Linux VM over SSH, using Docker Compose behind a Caddy reverse proxy with automatic HTTPS. Use whenever the user wants to self-host, install, set up, provision, or deploy n8n on their own server/VPS/box (Hetzner, DigitalOcean, AWS EC2, bare metal, etc.) — in either single/regular mode or queue mode with workers — or to update, back up, restore, or harden such an instance. This is for SELF-HOSTED n8n (Docker), not n8n Cloud and not building workflows. The skill makes the agent ask single-vs-queue first, collect the domain/SSH/timezone inputs, generate fresh secrets on the box, and bring the stack up with TLS. Trigger on "deploy n8n", "self-host n8n", "install n8n on my server", "n8n docker compose", "n8n queue mode / workers / scaling", "n8n reverse proxy / SSL", "back up / update my n8n", or "we don't want to give every user the OAuth client secret" / "enable the Sign in with Google button" (credential overwrites).

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Spawns vehicles on a running CARLA server and destroys them — scattered across the map's spawn points on Traffic Manager autopilot, a row queued in a single lane at a fixed spacing, or a single hero/ego vehicle (optionally named for native ROS 2 publishing with --ros-name). Covers blueprint filtering, the atomic autopilot hand-off, and cleanup. Use when the user asks to "spawn vehicles/cars/traffic", "add N cars driving around", "put cars in a lane 15 m apart", "spawn the ego/hero vehicle", or "remove the vehicles".

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Comprehensive guide for writing Apache Cassandra in-JVM distributed tests (dtests). Use when creating tests that simulate multi-node Cassandra clusters within a single JVM for faster integration testing. Covers cluster creation (single-node, multi-node, multi-datacenter), configuration (all cassandra.yaml parameters, features, network topology), instance lifecycle (startup/shutdown/restart), query execution, message filtering for failure scenarios, running code on instances, ClusterUtils utilities, and debugging classloader-related issues (serialization failures, same-class-different-classloader problems).

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Process, quality-control, annotate, and visualize single-cell or single-nucleus RNA-seq datasets across tissues and species. Use when Codex needs to build, adapt, or review a general scRNA-seq QC pipeline; choose dataset-appropriate cell-level filters from QC distributions; run required scDblFinder-based doublet and ambient-RNA filtering; annotate cells with matched references or marker-based fallbacks; or generate global and per-group UMAP visualizations for large scRNA-seq datasets.

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Route single-cell or single-nucleus RNA-seq FASTQs to public count-generation workflows and defer post-count matrix QC, annotation, clustering, and UMAP analysis to the embedded scrna-seq-qc skill.

日本語の概要は準備中です。原文の説明を表示しています。

Comprehensive web quality audit covering performance, accessibility, SEO, and best practices in a single review. Use when asked to "audit my site", "review web quality", "run lighthouse audit", "check page quality", or "optimize my website" across multiple areas at once. Orchestrates specialized skills for depth. Do NOT use for single-area audits — prefer core-web-vitals, web-accessibility, seo, or web-best-practices for focused work.

日本語の概要は準備中です。原文の説明を表示しています。

Deploy a production self-hosted n8n end-to-end to a fresh Linux VM over SSH, using Docker Compose behind a Caddy reverse proxy with automatic HTTPS. Use whenever the user wants to self-host, install, provision, or deploy n8n on their own server/VPS (Hetzner, DigitalOcean, AWS EC2, bare metal) — single/regular mode or queue mode with workers — or to update, back up, restore, or harden such an instance, or make Python Code nodes run on it (task runners). For SELF-HOSTED n8n (Docker), not n8n Cloud and not building workflows. The skill makes the agent ask single-vs-queue first, collect domain/SSH/timezone inputs, generate fresh secrets on the box, and bring the stack up with TLS. Trigger on "deploy n8n", "self-host n8n", "n8n docker compose", "n8n queue mode / workers", "n8n reverse proxy / SSL", "back up / update my n8n", "Python runner unavailable" / "n8nio/runners sidecar", or "we don't want to give every user the OAuth client secret" / "enable Sign in with Google" (credential overwrites).

日本語の概要は準備中です。原文の説明を表示しています。

Scrapes tweets from any X (Twitter) URL — search results, user profile, single tweet detail, or list timeline — and returns normalized per-tweet data with text, author, engagement counts, media, hashtags, mentions, and cursor for pagination. Use when user mentions X URL scraper, Twitter URL scraper, scrape from URL, given an X link, given a Twitter link, accept mixed X URLs, accept mixed Twitter URLs, search URL, profile URL, list URL, status URL, tweet detail URL, scrape startUrls Twitter, X startUrls, mixed batch of Twitter links, paste links and scrape, mass URL extraction X, mass URL extraction Twitter, scrape from a list of links, x.com URLs, twitter.com URLs, classify Twitter URLs, auto-detect Twitter URL type, scrape lists on X, scrape Twitter lists, list timeline scraper, list tweet scraper, scrape conversation by URL. Also applies to processing seed URL files, dispatching mixed X URLs through a single workflow, list-based KOL monitoring, batch-collecting tweets from a curated list of profiles, lists, and individual posts.

日本語の概要は準備中です。原文の説明を表示しています。