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概要と使いどころ
Extract methylation calls from Bismark BAM files using bismark_methylation_extractor. Generates per-cytosine reports for CpG, CHG, and CHH contexts. Use when extracting methylation levels from aligned bisulfite sequencing data for downstream analysis.
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Extract, filter, annotate, and export differential expression results from DESeq2 or edgeR. Use for identifying significant genes, applying multiple testing corrections, adding gene annotations, and preparing results for downstream analysis. Use when filtering and exporting DE analysis results.
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Parse and analyze multiple sequence alignments using Biopython. Extract sequences, identify conserved regions, analyze gaps, work with annotations, and manipulate alignment data for downstream analysis. Use when parsing or manipulating multiple sequence alignments.
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Preprocesses cell-free DNA sequencing data including adapter trimming, alignment optimized for short fragments, and UMI-aware duplicate removal using fgbio. Applies cfDNA-specific quality thresholds and fragment length filtering. Use when processing plasma cfDNA sequencing data before downstream analysis.
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ChIP-seq quality control metrics including FRiP (Fraction of Reads in Peaks), cross-correlation analysis (NSC/RSC), library complexity, and IDR (Irreproducibility Discovery Rate) for replicate concordance. Use to assess experiment quality before downstream analysis. Use when assessing ChIP-seq data quality metrics.
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Build, migrate, and deploy Next.js apps on Cloudflare Workers with vinext. Use when starting a Next.js project on Cloudflare, moving an existing app to Workers, choosing between vinext and OpenNext, or setting up vinext for Workers. For setup, migration, or deployment, install vinext's upstream skills with `npx skills add cloudflare/vinext` if missing, then read and follow the applicable skill and docs.
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Build and troubleshoot Cloudflare Basin analytics workflows with Basin Pipelines, Basin Catalog, and Basin SQL. Use for streaming data into R2 Iceberg tables, managing catalogs, or querying those tables; also use for requests using the former Data Platform, Pipelines, R2 Data Catalog, or R2 SQL names.
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Apply Martin Kleppmann-inspired data-system rules when designing reliability, scalability, consistency, replication, transactions, streams, or schema evolution.
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Extract edge hints from daily market observations and news reactions, with optional LLM ideation, and output canonical hints.yaml for downstream concept synthesis and auto detection.
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Use when the user asks to "codify how our founder sounds", "build a founder voice dossier", or "set our content pillars"; runs an 80%-extraction interview over the user's OWN posts, emails, and decks (never competitor scraping, never an invented persona) and produces the versioned voice record — a per-platform register map (incl. 小红书/微信公众号), banned phrases, per-context disclosure lines (the ECHO C2 upstream), a few-shot bank built exclusively from own posts, and 3-5 content pillars with Estimated %-allocations — submitted via memory/events/channels.ndjson for channel-registry to store as voice-dossier.md, the record every Craft-phase skill reads first. Not for the brand-level voice canon — use brand-language-codifier; not for audience/persona research — use audience-mapper. 声音档案/品牌语气/创始人语气/内容支柱/披露声明
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Use when the user asks to "measure how our narrative is landing", "track echo rate against our canon lexicon", or "check how AI answer engines describe our brand"; produces a resonance report — echo rate (overlap of market language with the narrative-registry canon lexicon, method declared), AI-answer perception via tavily.py --answer (proxy-labeled), share-of-voice on a locked competitor panel (reusing share-of-voice-tracker), and resonance signals from bluesky.py / gdelt.py / pageviews.py — every number labeled Measured / proxy / User-provided, feeding the TALE E dimension and the upstream of the E1 evidence-integrity veto. Not for rebuilding share-of-voice machinery — use share-of-voice-tracker; not for own-site GA4/GSC analytics — use performance-monitor; not for scoring TALE profile result — use narrative-quality-auditor; not for adjudicating claims — use offer-claims-registry. 回声率/AI回答感知/份额之声/共鸣信号
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Use when the user asks to "map our positioning", "name our competitive alternatives", or "pick a beachhead segment for the launch"; produces a Dunford-style positioning canvas — named competitive alternatives (including spreadsheet and status quo), unique attributes (verifiable, or routed to the claims ledger), value themes (attribute→benefit→value chains), a target beachhead segment scored on serviceability / pain intensity / reachability, and a one-sentence onlyness statement — the sole upstream of the message house and the entity-signal source for the canonical entity profile. Not for the message house or per-channel launch copy — use message-house-builder; not for audience/persona profiling itself — use audience-mapper; not for SEO keyword positioning — use keyword-research. 定位画布/竞争替代品/独特价值/滩头细分
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Use when the user asks to "monitor my launch", "track our Product Hunt / Hacker News ranking", or "watch the launch window"; runs the T-0 to T+30 window watch — pre-launch instrumentation verification (UTM/event checks, the upstream of RAMP P1), HN rank/points/comments polling with a comments-over-points flamewar early-warning (Estimated heuristic), PH votes/featured status, store charts and reviews, news echo, D0/W1/M1 KPI snapshots vs targets, spike-vs-sustain and owned-capture reads, and alert thresholds against the launch-tier KPI targets. Not for launch-day go/rollback calls — use launch-day-conductor; not for metric deep-dives — use performance-analyzer; not for SEO rank tracking — use rank-tracker. 发布监控/排名轮询/火焰战比/spike-sustain
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Use when the user asks to register/query a social channel, record channel state, cadence, governance, voice adaptation, UGC permission, or advocacy facts; curates them through the append-only channels event stream and derived views. Not for ECHO scoring — use social-quality-auditor; not for channel selection — use channel-portfolio-planner. 渠道台账/账号档案/UGC授权记录
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Use when the user asks "what did we pay this creator last time" or to "update the creator roster"; curates creator identity, rate, rights, exclusivity, compliance-event, and performance facts through the append-only creators event stream. Not for scoring fit — use fit-scorer; not for reviewing content — use creator-content-auditor. 创作者档案/达人名册
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Use when the user asks to record/query the brand narrative canon, tagline, message hierarchy, voice/naming rules, or a canon re-version; curates complete versioned canon events through the append-only narrative stream and derived views. Not for TALE scoring — use narrative-quality-auditor; not for authoring the system — use message-system-architect. 品牌叙事台账/canon 记录/语气与命名规范
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Use when the user asks to "log this subscriber's opt-in", record unsubscribes/complaints, or query lawful basis; curates pseudonymous consent facts through the append-only consent stream and applies suppression/erasure tombstones immediately. Not for SEND scoring — use email-quality-auditor; not for building segments — use list-segment-builder. 订阅同意台账/实时退订抑制/合法性依据登记
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Use when the user asks to "register this claim", "log our current offers", or "where is the proof for this figure"; curates claim wording, evidence, disclosures, terms, review dates, and live offers through the append-only claims event stream. Not for scoring claim vetoes — use the relevant auditor; not for writing ad copy — use ad-creative-builder. 广告声明台账/优惠信息登记/证据溯源
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Use when the user asks to "log this launch", query a launch date/embargo, record a stage transition, or update submissions/outcomes; curates launch facts through the append-only launches event stream with optimistic revisions and derived dossier/calendar views. Not for RAMP scoring — use launch-readiness-auditor; not for planning tier/window — use launch-tier-planner. 发布台账/发布日历/阶段与禁运期记录
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Debugs AWS Lambda function timeout failures by systematically analyzing function configuration, CloudWatch logs and metrics, VPC/networking, cold starts, memory constraints, and downstream dependencies to identify root causes with actionable fixes. Use when a Lambda function is timing out or approaching its timeout limit.
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Use to extend an existing agent project with memory, app integration, VPC, multi-agent, migration, model, browser, code interpreter, payments, or resource removal. Triggers: "add memory", "remember across sessions", "call agent from app", "invoke agent from code", "agent auth", "streaming", "VPC", "VPC connectivity", "can't reach from VPC", "multi-agent", "A2A", "A2A auth", "orchestrator not delegating", "specialist not called", "migrate Bedrock Agent", "migration issue", "change model", "browser tool", "code interpreter", "delete agent", "tear down", "agentcore remove", "cross-account memory", "add payments capability to my agent", "wire payments plugin", "integrate x402 payments with the agent I'm building", "add MPP payments", "Machine Payments Protocol". External APIs via Gateway: use agents-connect. New project: use agents-get-started. CLI/dev-server errors: use agents-debug. Runtime x402/MPP payments: use agents-pay. Migration-specific Strands vs LangGraph routes here.
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Run a deep root-cause investigation on the AWS DevOps Agent. Use when the user describes an incident, alarm, outage, or unexplained behavior — keywords like "5xx", "503", "OOM", "latency spike", "deployment failure", "rollback", "sev1", "investigate", "root cause", "debug", "alarm fired", "service down". Polls and streams progress, then surfaces recommendations.
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Evaluates, configures, and migrates workloads to AWS Lambda Managed Instances (LMI). Runs Lambda Event Functions (standard Lambda) on EC2 instances in the user's account while AWS manages provisioning, patching, scaling, routing, and load balancing. Triggers when queries mention Lambda Managed Instances, LMI, capacity providers, multi-concurrent execution environments, EC2-backed Lambda, persistent Lambda instances, PerExecutionEnvironmentMaxConcurrency, CapacityProviderConfig, cold start elimination via dedicated instances, migrating Lambda Event Functions to managed instances, or cost comparison between Lambda Event Functions and LMI with Savings Plans or Reserved Instances. Also covers long-running and asynchronous workloads and the 90-minute (5400s) function timeout for asynchronous and event-source-mapping (SQS, Kinesis, DynamoDB Streams / ESM) invocations, including how to raise the function timeout up to 90 minutes / 5400s and the related duration limits.
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Builds, runs, debugs, and operates event-driven applications using EventBridge Event Bus - a managed, centrally governed publish/subscribe event bus that an organization can share across many teams and accounts. Applicable when workloads need event-driven architectures, decoupling, choreography, asynchronous integration, pub/sub, fan-out, event ordering, delivered in sequence, event router, event broker, event bus, event store, event retention, event replay, deduplication, avro, protobuf, cloudevents, uses events or messages. Designed as a centrally managed event bus platform allowing governance and control - such as subscriber control, revocation, per-account throttling - while giving application owners flexibility across an organization spanning multiple accounts, with end-to-end open-standards observability and cost allocation. Also helps reduce costs in high fan-out scenarios where multi-account forwarding adds cost. A serverless, managed alternative to self-managed event streaming platforms.
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