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概要と使いどころ
Analyzes isoform switching events and functional consequences using IsoformSwitchAnalyzeR. Predicts protein domain changes, NMD sensitivity, ORF alterations, and coding potential shifts between conditions. Use when investigating how splicing changes affect protein function.
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Identifies super-enhancers from H3K27ac ChIP-seq data using ROSE and related tools. Use when studying cell identity genes, cancer-associated regulatory elements, or master transcription factor binding regions that cluster into large enhancer domains.
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Apply Martin Fowler-inspired enterprise architecture rules when choosing layers, service layers, transaction scripts, domain models, repositories, mappers, or DTOs.
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Use when the user asks to "analyze backlinks", "analyze my off-site signals", or "track AI traffic / ChatGPT / Perplexity referrals"; profiles referring domains, anchor-text mix, toxic links, and disavow candidates (backlinks mode) or isolates AI-assistant referral sessions in GA4/GSC/logs and reports their trend, landing pages, and AI-vs-organic conversion (ai-referrals mode). Not for internal links — use site-structure-optimizer; not for keyword positions — use rank-tracker; not for multi-metric stakeholder reports — use performance-monitor. 外链分析/反向链接/AI流量/AI引荐/ChatGPT流量/AI转化
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Use when the user asks to "build programmatic SEO pages", "generate pages at scale", "rank on a high-authority third-party site", "borrow domain authority", "build a vs / alternative page", "do local SEO", "optimize a Google Business Profile", or "fix NAP" — one page-build router with four modes: programmatic (template × dataset systems), parasite (barnacle publishing on Medium/Reddit/LinkedIn/GitHub), comparison (vs / alternative pages in four formats), and local (GBP + NAP + citations + location pages), each with its own thin/duplicate, honesty, or reputation-abuse guardrail. Not for finding what to target — use keyword-research or content-gap-analysis; not for drafting one standalone article — use content-writer; not for scoring a page for publish — use content-quality-auditor. 程序化SEO/批量页面/寄生SEO/借势权重/对比页/vs页/替代页/本地SEO/谷歌商家档案/NAP一致性
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Use when the user asks to "build a B2B cold-outbound sequence", "design reply-triage branching", "plan a domain warmup / sending throttle", or "make my outbound CAN-SPAM / opt-in compliant"; produces a multi-step outbound sequence with reply-triage branches (positive / objection / referral / not-now / opt-out), a warmup + send-throttle ramp schedule, jurisdiction opt-in/CAN-SPAM guardrails (guidance, not legal advice), and a SEND S-dimension read. Not for B2C lifecycle flows — use email-sequence-designer; not for the consent record — use consent-registry; not for computing EQS — use email-quality-auditor. B2B冷启动外联序列/回复分流/域名预热
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Use when the user asks to "run a deliverability pre-flight before I send", "check my SPF/DKIM/DMARC/BIMI", "why am I landing in spam / promotions", or "score my sender reputation and list hygiene"; runs the ONE-TIME pre-send SEND S1 authentication pre-flight and builds the SEND S (Sender-integrity / Deliverability) evidence read — DNS + DMARC-RUA auth, domain/IP reputation, inbox placement, content/link/render, and point-in-time bounce/complaint hygiene — using Pass/Partial/Fail/Unknown/N/A states and scoring only at complete applicable coverage. Not for the recurring hygiene trend — use list-hygiene-monitor; not for final EQS or veto verdicts — use email-quality-auditor; not for segments/suppression lists — use list-segment-builder. 邮件送达率预检/SPF DKIM DMARC认证/发件域声誉
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Use when the user asks to "track where my emails are actually landing after I send", "read my seed-list inbox vs spam vs promotions results", "trend my Gmail Postmaster / Microsoft SNDS reputation", or "did placement drop after my last send"; produces a per-provider inbox/spam/promotions placement read, a domain/IP reputation trend from Postmaster + SNDS, a send-over-send delta with named regressions, and a reusable SEND-S placement snapshot on your own exported telemetry. Not for the pre-send SPF/DKIM/DMARC auth pre-flight — use deliverability-qa; not for computing the EQS or running the vetoes — use email-quality-auditor. 邮件收件箱落点监测/收件箱vs垃圾邮件/Postmaster声誉趋势
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Guide for working in the grails-data-hibernate7 module, especially Hibernate 7 domain binding, mapping migration, generators, and integration tests. Use this when changing code or tests under grails-data-hibernate7.
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Run an expert review against Nielsen's heuristics and domain criteria, with severity ratings. Use when you need findings without recruiting participants. For a facilitated team feedback session, use `design-critique` (design-ops).
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Capture a user-reported defect as a durable GitHub issue written in the project's own domain language. Explores the codebase in parallel for context but never leaks file paths or line numbers into the issue. Use when the user reports a bug conversationally, runs a QA pass, or says "file an issue", "log this as a bug", "capture this".
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Sets up CloudWatch observability for the first time - Omni (CloudWatch Application Observability) and classic CloudWatch. Omni: creating a Space or Domain; access grants (who has access, at what level) and access profiles bounding async alerts, integrations, or agents; instrumenting an app or AI agent with the plain ADOT SDK so traces reach Omni (Python/Node/Java/.NET on EC2/ECS/EKS/Lambda), incl. no-image-rebuild and .NET CoreCLR vars; ingesting Azure telemetry via the CloudWatch agent on an Azure VM or AKS; connecting Slack to a Space; whether GitHub or a custom MCP tool server (HTTP/stdio; API key, bearer, OAuth2) can be connected. CloudWatch: onboarding a service to Application Signals - ADOT auto-instrumentation, the amazon-cloudwatch-observability add-on, monitored service, reporting telemetry, ServiceEvents, CI/CD git/deployment metadata, Terraform/manifest edits. For using what is set up - queries, dashboards, alarms, Omni alerts, X-Ray, synthetics, Dynamic Instrumentation - use aws-observability.
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Configures Amazon Route 53 DNS: public and private records, traffic-steering routing policies, health checks, DNS Firewall, Route 53 Profiles, VPC Resolver (also known as Route 53 Resolver) for hybrid and Outposts networks, and Global Resolver. Applicable when the customer wants to point a hostname at a target, split or fail over traffic across endpoints, monitor an endpoint, block malicious domains, centralize DNS across accounts, or resolve private DNS across a hybrid network. Routes to the right per-task procedure in references. Does not cover CloudFront-specific setup (see the route53-cloudfront skill) or non-DNS networking.
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Guides Amazon SES onboarding for domain-based email sending. Covers identity configuration, production access, an optional first test send, and troubleshooting setup, authentication, or sending failures. Use when setting up SES, resuming incomplete onboarding, or leaving the sandbox. Does not cover inbound email or Mail Manager, SMS/voice, WhatsApp, SNS, Pinpoint, or WorkMail.
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Connects AI agents to remote Windows desktop applications on Amazon WorkSpaces Applications (AppStream 2.0) through the managed Agent Access MCP server, and guides reliable desktop automation. Covers connecting an agent to the MCP endpoint (SigV4, streaming URL, and Active Directory SAML/Domain Join), BLOCKING vs POLLING connect modes, the computer-use tools (screenshot, click, type, key, scroll), screenshot-budget and action-batching discipline, MCP tool forwarding (forwarded___ tools), session lifecycle and expire-on-delete, and troubleshooting connection errors. Use when building or debugging an agent that drives a remote Windows desktop or GUI application via WorkSpaces Applications / AppStream — including "dcv session not ready", "client_disconnected", 400 signing-region, POLLING/connection_status, SAML assertion, or forwarded tool questions. Not for Amazon WorkSpaces Personal/Core virtual desktops or general AppStream fleet administration unrelated to agent access.
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Guides migration, provisioning, search, log-analytics, trace-analytics, and Agentic AI Assistant workflows for Amazon OpenSearch Service and Serverless across six capabilities — migration (Solr/ES/self-managed into AOS/AOSS, schema/query translation, sizing, cutover); provisioning (domain + AOSS lifecycle, upgrades, FGAC, monitoring); search (vector / semantic / hybrid / RAG with Bedrock); log-analytics (PPL, OSI, anomaly detection, Dashboards); trace-analytics (OTel spans, service maps, Data Prepper); ai-assistant (natural language data exploration, incident investigation, root cause analysis). Triggers on OpenSearch, AOS, AOSS, Elasticsearch, Solr, vector/k-NN/semantic/hybrid search, RAG, log analytics, PPL, trace analytics, ISM, FAISS, HNSW, Migration Assistant, UltraWarm, OR1, query my data, analyze logs, investigate errors, root cause analysis.
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Audit a target's CORS posture — Access-Control-Allow-Origin handling, reflected-origin bypass, credentials+wildcard mismatch, preflight OPTIONS behavior, Vary header correctness. Use when: a third-party integration is failing CORS preflight and someone proposes "just set Allow-Origin to *" as the fix, OR your bug-bounty inbox has a credential-reuse exploit chain. Threshold: any reflection of arbitrary Origin into Allow-Origin, Allow-Credentials:true with wildcard origin (browser-rejected combo but server config wrong), missing Vary:Origin on per-origin responses, preflight cached over 86400s, OR Allow-Origin trust of attacker- controlled subdomain pattern. Trigger with: "audit cors", "check cors policy", "cors bypass", "preflight check".
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Analyze HTTP security headers of web domains to identify vulnerabilities and misconfigurations. Use when you need to audit website security headers, assess header compliance, or get security recommendations for web applications. Trigger with phrases like "analyze security headers", "check HTTP headers", "audit website security headers", or "evaluate CSP and HSTS configuration".
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Organization-grade cloud and supply-chain attack-surface discovery: S3/GCS/Azure Blob bucket discovery via observed-name mining (CNAME/cert-SAN/Wayback) and bounded two-class permutation (6 prefixes x 15 suffixes on trusted tokens, bounded target-bound expansion on subdomain stems), existence (HEAD/GET) vs public-listing confirmation, object-key triage into 9 value tiers (database dumps, credentials, IaC state, kubeconfig, VCS dirs, config, archives, PII, logs), dangling-CNAME bucket-takeover detection, and the ownership-gated severity model that stops an unattributable public bucket from becoming a false CRITICAL; the fully offline AWS-account-ID recovery from a leaked AKIA/ASIA/AROA access key (base32 decode, runnable stdlib Python, canonical test vector, AWS-documentation-example-ID screening); dependency-confusion confirmation for npm/PyPI (internal-signal classifier -- private-registry binding vs org-namespace match -- paired with a read-only public-registry 404 check and the npm scope-claimability nuance the public search API misses); and passive cloud-native/container/Kubernetes/CI control-plane fingerprinting (Lambda URLs, API Gateway, Cloud Run, App Service, kubelet/etcd/K8s API/dashboard, Jenkins/GitLab/Argo CD) as an org-attribution and exposure surface. Passive/discovery only -- no exploitation, no credential submission, no active control-plane confirmation (a stage-6 validate_cloud active tier is described but out of scope). Use when enumerating a target's cloud storage footprint, recovering an AWS account ID from a leaked key, confirming a supply-chain dependency-confusion vector, or fingerprinting cloud-native/K8s/CI infrastructure for an authorized external recon engagement.
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End-to-end external OSINT engagement autopilot. Run the FULL osint-methodology pipeline to completion in ONE go for an authorized domain — engagement folder, Stages 1-5 (seed, expansion, enrichment, exposure, convergence), multi-agent per-host content+JS fan-out, headline verification, auto-generated findings, and a consolidated multi-tab .xlsx deliverable. Stops ONLY for Stage 6 (active exploitation) arming and out-of-scope sibling assets. Use whenever asked to "run OSINT / recon / attack-surface" on a target — do NOT deliver a thin passive first pass.
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Doctrine for drafting and keeping working-group docs under `docs/wg/**` — RFC/RFD specs and findings/research/glossary. A WG doc is a language-agnostic, code-agnostic study of a domain: it argues *why* and defines *what*, never *how in our code*. Use when writing or editing anything under `docs/wg/`, an RFC/RFD, a spec, a design note, a glossary, or research findings — including "write up the design", "document the spec", or "capture what we learned". Not for plans/TODOs (untracked `*.plan.md`), user docs, or SDK API refs — use `docs` to route those.
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Names and rebrands an app clone so it is the user's own: name candidates with the trademark, domain, store and handle checks to run, a new palette checked for contrast, a logo brief, a voice guide, and a sweep tool that finds anything of the original left in the codebase. Use when the user says "name my app", "rebrand the clone", "make it mine", "pick a name and colours", "logo brief", "brand voice", "check for leftovers", or after /replica-entrepreneur. Always runs before /replica-launch.
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Comprehensive guide for creating software diagrams using Mermaid syntax. Use when users need to create, visualize, or document software through diagrams including class diagrams (domain modeling, object-oriented design), sequence diagrams (application flows, API interactions, code execution), flowcharts (processes, algorithms, user journeys), entity relationship diagrams (database schemas), C4 architecture diagrams (system context, containers, components), state diagrams, git graphs, pie charts, gantt charts, or any other diagram type. Triggers include requests to "diagram", "visualize", "model", "map out", "show the flow", or when explaining system architecture, database design, code structure, or user/application flows.
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Conduct comprehensive, systematic literature reviews using multiple academic databases (PubMed, arXiv, bioRxiv, Semantic Scholar, etc.). This skill should be used when conducting systematic literature reviews, meta-analyses, research synthesis, or comprehensive literature searches across biomedical, scientific, and technical domains. Creates professionally formatted markdown documents and PDFs with verified citations in multiple citation styles (APA, Nature, Vancouver, etc.).
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